Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F200 I1 R1
|
100 |
75.0 |
3643689 |
96.7% |
3523447 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,967,707 |
T→A |
D243V (GAT→GTT) |
ptsP ← |
PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,967,707 | 0 | T | A | 97.1%
| 232.3
/ ‑1.2
| 69 | D243V (GAT→GTT) | ptsP | PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (39/28); total (40/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 |
CCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATCGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTC > NC_000913/2967612‑2967803
|
ccGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCAACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGaa > 2:1374019/1‑100 (MQ=255)
cGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGaaa > 2:261942/1‑100 (MQ=255)
gTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGaaaa > 2:1573763/1‑100 (MQ=255)
tACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgcc > 1:1286982/1‑100 (MQ=255)
tACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgc > 2:667606/1‑99 (MQ=255)
aCCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgccg > 2:518773/1‑100 (MQ=255)
ccGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgccgc < 1:1654843/100‑1 (MQ=255)
ccGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgccgc > 2:713714/1‑100 (MQ=255)
ccGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGACCGAAAGCAGGTGCGAGTAAAGAACGAAAATacac > 1:1463009/1‑95 (MQ=255)
cGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAgccgcc > 2:591762/1‑100 (MQ=255)
ccACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTtg < 1:627978/100‑1 (MQ=255)
ccACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTtg > 2:1686616/1‑100 (MQ=255)
cACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTtgt < 1:775386/100‑1 (MQ=255)
cACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTtgt > 2:355897/1‑100 (MQ=255)
aCCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTtgtg < 1:531198/100‑1 (MQ=255)
gCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcg > 2:553578/1‑100 (MQ=255)
ccTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcgg < 1:268630/100‑1 (MQ=255)
cTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcggc > 2:1652020/1‑100 (MQ=255)
ttATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcggcgg < 2:562565/100‑1 (MQ=255)
ttATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcggcgg > 1:782179/1‑100 (MQ=255)
tATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCggcggc < 1:348393/100‑1 (MQ=255)
aTCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGACAGCAGGTGCGAGTAACGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACcgg > 1:1706877/1‑95 (MQ=255)
aTCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCa > 1:311952/1‑100 (MQ=255)
aCCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATCGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGc > 2:837003/1‑100 (MQ=255)
aCCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGACGGCAAAGc > 2:743982/1‑100 (MQ=255)
ccTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCg > 2:1095754/1‑100 (MQ=255)
ccTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCg < 2:202138/100‑1 (MQ=255)
ccTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCg < 1:1480469/100‑1 (MQ=255)
cTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGt > 1:1414058/1‑100 (MQ=255)
cTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGt < 2:1189645/100‑1 (MQ=255)
cGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGttt < 1:1014610/100‑1 (MQ=255)
cGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCAGCGGCAAAGCGttt > 2:1467004/1‑100 (MQ=255)
gCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGc < 2:1015309/100‑1 (MQ=255)
aaaCAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTg < 1:1283852/100‑1 (MQ=255)
aaCAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGt > 2:924142/1‑100 (MQ=255)
aCAATTCGCGACGCAGCCGGTTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTa < 2:1010779/100‑1 (MQ=255)
aCAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTa > 1:1519139/1‑100 (MQ=255)
cAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAg > 1:492070/1‑100 (MQ=255)
aaTTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAgc > 1:1113138/1‑100 (MQ=255)
aaTTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAgc > 1:1348469/1‑100 (MQ=255)
aTTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAgcg > 1:748462/1‑100 (MQ=255)
ttCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAgcgg < 2:1436784/100‑1 (MQ=255)
tCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTCgcggc > 1:1605573/1‑100 (MQ=255)
cgACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGaa < 2:392936/100‑1 (MQ=255)
cgACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGaa > 2:319430/1‑100 (MQ=255)
cGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGAGTTTGCTGTAGCGGCGAAAc > 1:1656718/1‑99 (MQ=255)
gCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTc < 1:850798/100‑1 (MQ=255)
cAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGaaa > 1:1740062/1‑96 (MQ=255)
ccGGGTGTCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGttt < 1:324423/100‑1 (MQ=255)
ggTGTCCGAAAGCAGGTGCGAGTAAAGAACGCAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGcc < 1:82140/100‑1 (MQ=255)
gtgtCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCg < 2:1739859/100‑1 (MQ=255)
tgtCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGc > 2:1354121/1‑100 (MQ=255)
gtCCGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGct < 2:336747/100‑1 (MQ=255)
ccGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGcttc > 1:314572/1‑100 (MQ=255)
cGAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGcttct > 1:154217/1‑100 (MQ=255)
gAAAGCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGcttctt > 2:1511736/1‑100 (MQ=255)
aGCATGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCa < 1:1722046/100‑1 (MQ=255)
gCAGGTGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAg < 1:1652009/100‑1 (MQ=255)
tGCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCAAAACTCGTTTGCCGCTTCTTCCAGCGccc > 2:39611/1‑100 (MQ=255)
gCGAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGcccc < 1:1275943/100‑1 (MQ=255)
gAGTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCgg < 1:261941/100‑1 (MQ=255)
gTAAATAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTGGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTc < 1:1672763/100‑1 (MQ=255)
gTAAAGATCGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTc < 1:836998/100‑1 (MQ=255)
gTAAAGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTc > 2:643927/1‑100 (MQ=255)
aaaGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAg > 2:1063000/1‑100 (MQ=255)
aaaGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTACCGCTTCTTCCAGCGCCCCGGTCAg > 2:140570/1‑100 (MQ=255)
aaGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGt < 1:1245964/100‑1 (MQ=255)
aaGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGt < 2:770133/100‑1 (MQ=255)
aGAACGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGAGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTc > 2:103563/1‑100 (MQ=255)
|
CCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATCGAAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTC > NC_000913/2967612‑2967803
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
