Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F200 I1 R1
|
100 |
75.0 |
3643689 |
96.7% |
3523447 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,834,428 |
(G)6→5 |
coding (110/2319 nt) |
yicI ← |
putative alpha‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,834,423 | 0 | G | . | 98.6%
| 316.2
/ ‑2.7
| 70 | coding (115/2319 nt) | yicI | putative alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (38/31); minor base A (1/0); total (39/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGT > NC_000913/3834327‑3834518
|
gCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACgggg < 1:263952/100‑1 (MQ=255)
cTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTCCGTTCACGCACATCACggggg > 1:747795/1‑100 (MQ=255)
cTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACggggg > 1:995807/1‑100 (MQ=255)
cTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACggggg > 1:305514/1‑100 (MQ=255)
cTCAATCCGCACACCGACAATACCTTACTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACggggg > 1:546584/1‑100 (MQ=255)
cAATCCCCACACCGACAATAACTACCTGTGGGGAGAAAAAGCGCAACGTAAATAACAGCGTATCAATATGCCAGGTACGTTCACACACACCACggaggc > 1:366303/1‑98 (MQ=255)
aTCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACgggggcagc < 1:945264/100‑5 (MQ=255)
aTCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACgggggcagc < 1:986423/100‑5 (MQ=255)
tCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGgcagca < 2:1487930/100‑1 (MQ=255)
ccGCACACCGACAATACCTTCCTGTGGGGGGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGGCAGGTACGTTCACGCACATCAC‑GGGGgcagca > 1:825716/1‑99 (MQ=255)
cGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATa > 1:180314/1‑100 (MQ=255)
cGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATa > 2:1045240/1‑100 (MQ=255)
cGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑‑GGGGCAGCATAg > 2:1134354/1‑100 (MQ=255)
acacCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGac > 1:819818/1‑100 (MQ=255)
cacCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGacc < 1:256325/100‑1 (MQ=255)
ccGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGaccac > 1:381071/1‑100 (MQ=255)
cGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGaccacc > 1:1705911/1‑100 (MQ=255)
aCAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCAt < 2:1599187/100‑1 (MQ=255)
aCAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCAt < 2:161047/100‑1 (MQ=255)
aaTACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCAttt < 1:610364/100‑1 (MQ=255)
aTACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTc > 2:1430615/1‑100 (MQ=255)
tACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCAttt > 2:412514/1‑98 (MQ=255)
cccctacTGTAGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATGAAGCTGCCAGGTACGTTCACGCACATGAC‑GGGGGCAGCAGAGACGACCATTTCAt > 1:1517171/7‑100 (MQ=255)
ccTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCAtt > 1:855724/1‑100 (MQ=255)
cTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTa < 2:1455704/100‑1 (MQ=255)
cTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCct < 1:390078/100‑1 (MQ=255)
gtgGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCctgc < 2:1799708/100‑1 (MQ=255)
gtgGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCctgc < 2:625911/100‑1 (MQ=255)
ggggAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCctgctg < 1:176924/100‑1 (MQ=255)
gggAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGt > 1:1778130/1‑100 (MQ=255)
agaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCa < 2:747907/100‑1 (MQ=255)
agaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCa < 1:92590/100‑1 (MQ=255)
agaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGACAACATAAAACACCATTTCATTATCCTGCTGttaa > 2:306333/1‑98 (MQ=255)
aaaaaGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCA‑AGGGGGCAGCATAGACCACCATTTCATTATACTGCTGTTCaa > 1:227334/1‑99 (MQ=255)
aaaaGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAAcc > 1:820127/1‑100 (MQ=255)
aaaaGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAAc > 2:1537737/1‑99 (MQ=255)
aGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCg < 1:94355/100‑1 (MQ=255)
gcgcAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGa > 2:510844/1‑100 (MQ=255)
cgcAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGaa > 2:271912/1‑100 (MQ=255)
gcAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAac < 2:414787/100‑1 (MQ=255)
cAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAaca > 1:660643/1‑100 (MQ=255)
aCGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACAcc < 2:600572/100‑1 (MQ=255)
aCGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACAcc < 1:1593672/100‑1 (MQ=255)
gTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTg < 2:1655797/100‑1 (MQ=255)
taaataaaGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGa > 2:286975/1‑100 (MQ=255)
taaataaaGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGa < 1:1230318/100‑1 (MQ=255)
taaataaaGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGa > 2:1472082/1‑100 (MQ=255)
taaaGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCg > 2:191273/1‑100 (MQ=255)
aaaGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCgg < 1:164448/100‑1 (MQ=255)
aGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGt > 2:1470284/1‑100 (MQ=255)
aGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGt < 1:1430604/100‑1 (MQ=255)
ggCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTg < 2:723233/100‑1 (MQ=255)
ggCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTg > 1:29600/1‑100 (MQ=255)
gCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACAACTGAAGCAGgaga > 2:769786/1‑100 (MQ=255)
gTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAAt < 2:1669260/100‑1 (MQ=255)
tATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATc > 1:574643/1‑100 (MQ=255)
aTCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCa > 1:1426570/1‑100 (MQ=255)
tCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCaa > 1:933405/1‑100 (MQ=255)
aaGCTGCCAGGTACTTTCACGTACATCAC‑GGGGGTAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCaaa < 2:652083/99‑1 (MQ=255)
aaGCTGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAAt > 2:1489046/1‑100 (MQ=255)
tGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAg < 2:1426581/100‑1 (MQ=255)
tGCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAg < 2:381073/100‑1 (MQ=255)
gCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAgg < 2:1478781/100‑1 (MQ=255)
gCCAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAgg < 1:1622245/100‑1 (MQ=255)
ccAGGTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGc > 1:1343115/1‑100 (MQ=255)
gTACGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCACGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAgg > 2:114409/1‑100 (MQ=255)
cGTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATGTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTg > 1:971891/1‑100 (MQ=255)
gTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGa > 2:458931/1‑100 (MQ=255)
gTTCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGAGGGTGAATCAAATTGAGGCCAGGTTGa > 2:405805/1‑100 (MQ=255)
ttCACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACTTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGaa < 1:271911/100‑1 (MQ=255)
cACGCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAACCGGGTGAATCAAATTGAGACCAGGTTGa > 1:552179/1‑97 (MQ=255)
gCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAAc < 2:350325/100‑1 (MQ=255)
gCACATCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAAc < 2:1053925/100‑1 (MQ=255)
cacaTCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAAcc > 1:699011/1‑100 (MQ=255)
cacaTCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAAcc > 1:879070/1‑100 (MQ=255)
caTCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAg > 2:469382/1‑100 (MQ=255)
caTCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAg > 2:298784/1‑100 (MQ=255)
aTCAC‑GGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGt < 1:484471/100‑1 (MQ=255)
|
GCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGT > NC_000913/3834327‑3834518
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
