Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,851,014 C→T 41.9% G27S (GGC→AGC)  ilvN ← acetolactate synthase 1 small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,851,0140CT41.9% 88.5 / 175.8 147G27S (GGC→AGC) ilvNacetolactate synthase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base C (44/41);  new base T (38/23);  total (82/65)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 156 total aligned reads displayed.

GGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTC  >  NC_000913/3850915‑3851113
                                                                                                   |                                                                                                   
ggTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGcc                                                                                                     <  2:2652825/100‑1 (MQ=255)
  tcgtcATTGACCAGTAGCCAGATATGGCTTTTTTCGCTGTCCTGAATCGGCCGACAAAGAATGCCTTCAACGATAAAAGCGCGGCGGGCAAAAAGGCaac                                                                                                   >  1:1658695/1‑100 (MQ=255)
   cgtcATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCcaca                                                                                                  >  1:2782333/1‑100 (MQ=255)
   cgtcATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCcaca                                                                                                  >  1:1531610/1‑100 (MQ=255)
       aTTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAAGGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCGACAAACg                                                                                              <  1:1760337/100‑1 (MQ=255)
         tGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTg                                                                                            <  2:1204109/100‑1 (MQ=255)
         tGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTg                                                                                            <  1:124717/100‑1 (MQ=255)
          gACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTgg                                                                                           <  1:288272/100‑1 (MQ=255)
          gACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTgg                                                                                           <  2:1321093/100‑1 (MQ=255)
           aCCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTggg                                                                                          <  2:542371/100‑1 (MQ=255)
             cAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTc                                                                                        >  1:2568779/1‑100 (MQ=255)
              aGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCa                                                                                       <  2:1623866/100‑1 (MQ=255)
              aGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCa                                                                                       <  2:412011/100‑1 (MQ=255)
               gTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCAt                                                                                      >  1:100015/1‑100 (MQ=255)
               gTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCAt                                                                                      >  2:2856580/1‑100 (MQ=255)
                tAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCAtt                                                                                     <  1:2709213/100‑1 (MQ=255)
                  gCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTAc                                                                                   <  1:2439857/100‑1 (MQ=255)
                  gCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTAc                                                                                   >  2:2620942/1‑100 (MQ=255)
                     aGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGcc                                                                                <  1:3417900/100‑1 (MQ=255)
                      gATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGccc                                                                               <  2:224169/100‑1 (MQ=255)
                      gATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGccc                                                                               <  1:2923389/100‑1 (MQ=255)
                        tatGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCgg                                                                             <  1:3302957/100‑1 (MQ=255)
                        tatGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCgg                                                                             >  1:2102409/1‑100 (MQ=255)
                        tatGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCg                                                                              <  2:1594606/99‑1 (MQ=255)
                         atGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGa                                                                            >  1:2727360/1‑100 (MQ=255)
                              ttttGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGtt                                                                       >  1:187912/1‑100 (MQ=255)
                                ttGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGc                                                                     <  2:146177/100‑1 (MQ=255)
                                 tGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCg                                                                    >  1:1514728/1‑100 (MQ=255)
                                  gTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAACGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCAGGATGGTTGCg                                                                    <  2:1506760/99‑1 (MQ=255)
                                  gTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCg                                                                    <  1:1694232/99‑1 (MQ=255)
                                    cGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAAc                                                                 <  2:3160506/100‑1 (MQ=255)
                                    cGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAAc                                                                 >  1:676743/1‑100 (MQ=255)
                                       tGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGt                                                              <  1:1559807/100‑1 (MQ=255)
                                        gTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACg                                                                >  1:320786/1‑97 (MQ=255)
                                        gTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTg                                                             <  1:606799/100‑1 (MQ=255)
                                        gTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTg                                                             <  2:2140421/100‑1 (MQ=255)
                                         tCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGa                                                            <  2:3091597/100‑1 (MQ=255)
                                          ccTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAg                                                           >  1:1479731/1‑100 (MQ=255)
                                           cTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGc                                                          <  1:896307/100‑1 (MQ=255)
                                           cTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGc                                                          <  2:2378423/100‑1 (MQ=255)
                                            tGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAgat                                                         >  2:677100/1‑98 (MQ=255)
                                            tGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCt                                                         >  2:1009709/1‑100 (MQ=255)
                                              aaTCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTcc                                                       >  2:2534825/1‑100 (MQ=255)
                                               aTCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCa                                                      <  1:228867/100‑1 (MQ=255)
                                                 cGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGa                                                    >  2:2030288/1‑100 (MQ=255)
                                                  ggCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGaa                                                   <  1:571958/100‑1 (MQ=255)
                                                  ggCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGaa                                                   >  2:1053077/1‑100 (MQ=255)
                                                  ggCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGaa                                                   >  1:2227808/1‑100 (MQ=255)
                                                  ggCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGaa                                                   >  1:1179535/1‑100 (MQ=255)
                                                   gCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAAt                                                  >  1:2770903/1‑100 (MQ=255)
                                                    cAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAAtt                                                 >  1:576374/1‑100 (MQ=255)
                                                    cAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAAtt                                                 >  2:2170839/1‑100 (MQ=255)
                                                     aGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTa                                                >  1:619110/1‑100 (MQ=255)
                                                     aGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTa                                                <  1:623833/100‑1 (MQ=255)
                                                     aGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTa                                                >  1:2958895/1‑100 (MQ=255)
                                                      gACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTAc                                               >  2:1421502/1‑100 (MQ=255)
                                                            gAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTc                                         <  1:3280241/100‑1 (MQ=255)
                                                             aaTGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTc                                         >  1:1104158/1‑99 (MQ=255)
                                                               tGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATg                                      <  1:307125/100‑1 (MQ=255)
                                                                 ccTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAg                                    <  2:576378/100‑1 (MQ=255)
                                                                 ccTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAg                                    >  2:3380045/1‑100 (MQ=255)
                                                                  cTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGt                                   >  1:2241005/1‑100 (MQ=255)
                                                                  cTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGt                                   >  1:1851806/1‑100 (MQ=255)
                                                                   ttCAACGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGt                                   >  2:875905/1‑99 (MQ=255)
                                                                    tCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGCCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTtg                                 >  2:457538/1‑100 (MQ=255)
                                                                      naCGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCGCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTtgtg                               >  1:3398275/2‑100 (MQ=255)
                                                                      aaCGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTtgtg                               >  2:2323387/1‑100 (MQ=255)
                                                                       aCGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTgtgt                              >  1:1003837/1‑100 (MQ=255)
                                                                        cGTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGtt                             >  1:2705072/1‑100 (MQ=255)
                                                                         gTTAAAAGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGttt                            <  1:403177/100‑1 (MQ=255)
                                                                         gTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGttt                            >  1:1094266/1‑100 (MQ=255)
                                                                            aaaaGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGc                         <  2:58691/100‑1 (MQ=255)
                                                                             aaaGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                        >  2:1964250/1‑100 (MQ=255)
                                                                             aaaGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                        >  2:1563239/1‑100 (MQ=255)
                                                                              aaGCGCGGCGGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCAt                       <  1:1589316/100‑1 (MQ=255)
                                                                               aGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATg                      >  2:816268/1‑100 (MQ=255)
                                                                                  gcggcgGGCAAAAAGTCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGc                    <  1:2416677/99‑1 (MQ=255)
                                                                                  gcggcgGGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCt                   <  2:3298336/100‑1 (MQ=255)
                                                                                  gcggcgGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCt                   <  2:1882543/100‑1 (MQ=255)
                                                                                   cggcggGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCtt                  >  2:3070391/1‑100 (MQ=255)
                                                                                    ggcggGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTa                 >  1:1016409/1‑100 (MQ=255)
                                                                                    ggcggGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTa                 <  1:1197063/100‑1 (MQ=255)
                                                                                     gcggGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTAt                >  2:912831/1‑100 (MQ=255)
                                                                                     gcggGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTAt                <  1:2039537/100‑1 (MQ=255)
                                                                                      cggGCAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTAtt               >  1:354303/1‑100 (MQ=255)
                                                                                        ggCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcc             <  2:2102426/100‑1 (MQ=255)
                                                                                        ggCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcc             >  2:1113638/1‑100 (MQ=255)
                                                                                         gCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccc            >  2:246631/1‑100 (MQ=255)
                                                                                         gCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccc            <  2:3355587/100‑1 (MQ=255)
                                                                                          cAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcccc           >  2:1605070/1‑100 (MQ=255)
                                                                                          cAAAAAGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcccc           >  1:3199786/1‑100 (MQ=255)
                                                                                          cAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcccc           >  2:726456/1‑100 (MQ=255)
                                                                                           aaaaaGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccccc          <  2:2820612/100‑1 (MQ=255)
                                                                                            aaaaGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCcca         >  2:1063302/1‑100 (MQ=255)
                                                                                            aaaaGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCcca         <  1:2620923/100‑1 (MQ=255)
                                                                                              aaGGCTACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCccacc       >  2:3401521/1‑100 (MQ=255)
                                                                                                ggCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTCCGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCAt     >  1:1015434/1‑100 (MQ=255)
                                                                                                ggCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTTATGAGTTGTGTTTTGCATGGCTTATTCCCcca         <  1:677096/96‑1 (MQ=255)
                                                                                                ggCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCAt     >  1:1903777/1‑100 (MQ=255)
                                                                                                   cacaAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTc  <  1:3380020/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTC  >  NC_000913/3850915‑3851113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A