Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I1 R1 118 120.6 6073099 93.5% 5678347 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,618,765 T→C Y60H (TAT→CAT)  nikR → transcriptional repressor, Ni‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,7650TC97.8% 473.3 / ‑6.5 134Y60H (TAT→CAT) nikRtranscriptional repressor, Ni‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (58/73);  minor base A (0/3);  total (58/76)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCA  >  NC_000913/3618669‑3618858
                                                                                                |                                                                                             
cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATg                                                                                            <  2:829651/100‑1 (MQ=255)
cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATg                                                                                            <  1:804044/100‑1 (MQ=255)
cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATg                                                                                            <  2:1385552/100‑1 (MQ=255)
cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATg                                                                                            <  1:2412346/100‑1 (MQ=255)
cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATg                                                                                            <  2:2732667/100‑1 (MQ=255)
 gTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGa                                                                                           <  1:2902464/100‑1 (MQ=255)
   tCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAac                                                                                         <  2:1423703/100‑1 (MQ=255)
     cGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAacac                                                                                       >  1:1615993/1‑100 (MQ=255)
     cGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAacac                                                                                       >  1:1149493/1‑100 (MQ=255)
     cGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAacac                                                                                       >  2:2557939/1‑100 (MQ=255)
      gAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACg                                                                                      >  1:2818809/1‑100 (MQ=255)
      gAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACg                                                                                      >  1:1997625/1‑100 (MQ=255)
      gAAGCTATCCGCAACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACg                                                                                      >  1:1995453/1‑100 (MQ=255)
       aaGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGa                                                                                     <  1:262567/100‑1 (MQ=255)
         gCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGaaa                                                                                   <  2:79257/100‑1 (MQ=255)
            aTCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGCGGCCACCCAGCCGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCCCGAACACGaaaa                                                                                  >  2:25693/1‑98 (MQ=255)
             tCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAAcg                                                                               <  2:2965395/100‑1 (MQ=255)
              ccGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAAcgc                                                                              >  2:1238967/1‑100 (MQ=255)
               cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGTACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAAcgcg                                                                             <  2:229944/100‑1 (MQ=255)
               cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAAcgcg                                                                             >  2:1533749/1‑100 (MQ=255)
                gcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGa                                                                            >  1:2229919/1‑100 (MQ=255)
                 cgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGAc                                                                           <  2:1517094/100‑1 (MQ=255)
                 cgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGAc                                                                           <  2:37587/100‑1 (MQ=255)
                 cgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGAc                                                                           <  1:1187374/100‑1 (MQ=255)
                  gACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACt                                                                          >  2:214101/1‑100 (MQ=255)
                     aTTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTag                                                                       <  1:54698/100‑1 (MQ=255)
                      ttCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGTAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGAAAAAAAAAGAAAAACGCGACTTagc                                                                      <  2:1671915/100‑1 (MQ=255)
                      ttCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagc                                                                      >  2:501227/1‑100 (MQ=255)
                      ttCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagc                                                                      <  2:1573999/100‑1 (MQ=255)
                       tCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcc                                                                     >  2:1493687/1‑100 (MQ=255)
                       tCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcc                                                                     >  2:1322732/1‑100 (MQ=255)
                       tCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcc                                                                     <  2:1510331/100‑1 (MQ=255)
                        cTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcca                                                                    >  1:2232609/1‑100 (MQ=255)
                        cTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcca                                                                    >  1:350312/1‑100 (MQ=255)
                            gtcgcgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCg                                                                <  2:2996550/97‑1 (MQ=255)
                            gTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCg                                                                <  1:1166736/100‑1 (MQ=255)
                              aGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCa                                                              >  1:3022391/1‑100 (MQ=255)
                              aGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCa                                                              >  1:355643/1‑100 (MQ=255)
                               gcgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCAt                                                             >  1:770955/1‑100 (MQ=255)
                                cgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCAtt                                                            <  2:2207891/100‑1 (MQ=255)
                                  cccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTg                                                           >  2:3034967/1‑99 (MQ=255)
                                  cccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGt                                                          >  2:1541552/1‑100 (MQ=255)
                                   ccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtc                                                         >  2:1119401/1‑100 (MQ=255)
                                   ccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtc                                                         >  1:1785503/1‑100 (MQ=255)
                                   catGGCGTCAGATGCCAGACAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTGGTATGTGCATGAACACGAAAAACGCGACTTAGCCATCCGCATTGtc                                                         <  2:2091094/98‑1 (MQ=255)
                                    cTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtct                                                        <  1:2229915/100‑1 (MQ=255)
                                     tGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAACCGCGACTTAGCCAGCCGCATTGtctc                                                       <  2:2737622/100‑1 (MQ=255)
                                     tGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtctc                                                       <  1:111258/100‑1 (MQ=255)
                                     tGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtctc                                                       <  1:1533762/100‑1 (MQ=255)
                                     tGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtctc                                                       <  1:308305/100‑1 (MQ=255)
                                      ggCGCAAGAGGCCACCCTGCAGCACGGCACGCAAGGTTTCGCGGGTCTGTCGTATGTGCATGAACACGATAAACGCGACTTAGCCAGCCGCATTgn                                                          <  2:1477487/96‑2 (MQ=255)
                                      ggCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTcc                                                      >  2:74550/1‑100 (MQ=255)
                                         gcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAcc                                                   <  1:1949779/100‑1 (MQ=255)
                                           aaGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCa                                                 <  1:2911842/100‑1 (MQ=255)
                                            agagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAg                                                >  2:1109414/1‑100 (MQ=255)
                                            agagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAg                                                >  2:295459/1‑100 (MQ=255)
                                             gagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGc                                               <  1:2462840/100‑1 (MQ=255)
                                             gagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGAAAAAAAAAAAAAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGc                                               <  2:474943/100‑1 (MQ=255)
                                              agGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGca                                              >  2:1052510/1‑100 (MQ=255)
                                               ggCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcat                                             >  2:366423/1‑100 (MQ=255)
                                                 ccACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcatca                                           >  1:523689/1‑100 (MQ=255)
                                                 ccACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcatca                                           <  1:1454836/100‑1 (MQ=255)
                                                 ccACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcatca                                           <  2:819583/100‑1 (MQ=255)
                                                  cACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcatcat                                          >  2:879040/1‑100 (MQ=255)
                                                    cccAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCAtcatca                                        >  1:2549976/1‑100 (MQ=255)
                                                    cccAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCAtcatca                                        >  1:2082860/1‑100 (MQ=255)
                                                     cctgcagcgCGGCACGCAAGGTTCCGCGGGGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCAtcatca                                        <  2:1804469/96‑1 (MQ=255)
                                                     ccAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGCGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATcac                                       >  1:1707188/1‑100 (MQ=255)
                                                         cagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACg                                   >  2:914319/1‑100 (MQ=255)
                                                         cagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACg                                   <  2:2283158/100‑1 (MQ=255)
                                                            cacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGAcc                                >  2:759504/1‑100 (MQ=255)
                                                            cacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGAcc                                <  1:702548/100‑1 (MQ=255)
                                                             acggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACct                               >  2:659237/1‑100 (MQ=255)
                                                               ggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACctct                             >  1:2290508/1‑100 (MQ=255)
                                                                     cAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCg                       <  2:1366079/100‑1 (MQ=255)
                                                                      aaGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGc                      <  1:1101730/100‑1 (MQ=255)
                                                                       aGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGcc                     <  1:302554/100‑1 (MQ=255)
                                                                        tccgatctggTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                    <  2:247735/92‑1 (MQ=255)
                                                                        ggTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGTCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                    >  2:174019/1‑100 (MQ=255)
                                                                        ggTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                    <  1:173933/100‑1 (MQ=255)
                                                                          tttCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACg                  >  2:2512241/1‑100 (MQ=255)
                                                                          tttCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACg                  >  2:221169/1‑100 (MQ=255)
                                                                            tCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCt                <  1:1571153/100‑1 (MQ=255)
                                                                            tCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCt                <  1:1878961/100‑1 (MQ=255)
                                                                            cttccgatcTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCt                <  1:1119133/92‑1 (MQ=255)
                                                                              gcgGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCt                <  2:1840895/98‑1 (MQ=255)
                                                                              gcgGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGc              >  1:637784/1‑100 (MQ=255)
                                                                               cgGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCa             <  1:2290463/100‑1 (MQ=255)
                                                                                ggTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCaagatcgga            >  1:247738/1‑92 (MQ=255)
                                                                                 gTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCAtg           <  2:2477792/100‑1 (MQ=255)
                                                                                   gCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCAtgtg         >  2:2612044/1‑100 (MQ=255)
                                                                                    cTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGc        <  1:759511/100‑1 (MQ=255)
                                                                                    cTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGGCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGc        <  2:2894262/100‑1 (MQ=255)
                                                                                     tGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGca       <  1:936489/100‑1 (MQ=255)
                                                                                     tGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGca       >  1:1486320/1‑100 (MQ=255)
                                                                                     tGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGca       <  1:2716230/100‑1 (MQ=255)
                                                                                      gTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcac      >  2:557815/1‑100 (MQ=255)
                                                                                      gTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcac      <  2:2319002/100‑1 (MQ=255)
                                                                                          tATGTGCATGGACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACTACTTCTCCGTCGCCACGCTGCATGTGCACATCa  <  2:1995435/100‑1 (MQ=255)
                                                                                          tATGTGCATGCACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCa  <  2:1997607/100‑1 (MQ=255)
                                                                                                |                                                                                             
CGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCA  >  NC_000913/3618669‑3618858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A