Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,618,765 T→C 25.3% Y60H (TAT→CAT)  nikR → transcriptional repressor, Ni‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,7650TC25.3% 246.3 / 95.6 142Y60H (TAT→CAT) nikRtranscriptional repressor, Ni‑binding
Reads supporting (aligned to +/- strand):  ref base T (42/64);  new base C (15/21);  total (57/85)
Fisher's exact test for biased strand distribution p-value = 8.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 148 total aligned reads displayed.

ACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACG  >  NC_000913/3618667‑3618864
                                                                                                  |                                                                                                   
acCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTa                                                                                                    <  1:2478132/100‑1 (MQ=255)
 cagTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCACAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTAt                                                                                                   >  2:2337601/3‑100 (MQ=255)
  cGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATg                                                                                                  <  2:1284593/100‑1 (MQ=255)
       cGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAacac                                                                                             >  2:449047/1‑100 (MQ=255)
       cGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAacac                                                                                             <  2:2119747/100‑1 (MQ=255)
          aGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaa                                                                                          <  2:948942/100‑1 (MQ=255)
          aGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaa                                                                                          <  2:3071389/100‑1 (MQ=255)
            cTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaata                                                                                        <  2:2341930/100‑3 (MQ=255)
            cTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaaaa                                                                                        <  2:793575/100‑1 (MQ=255)
            cTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGaaaa                                                                                        <  2:2832643/100‑1 (MQ=255)
             tATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaaaaa                                                                                       >  1:1559474/1‑100 (MQ=255)
             tATCCGCGACATTCTGCGTAGCGCCCGGGCGGAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGaaaa                                                                                        <  1:674541/99‑1 (MQ=255)
               tCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcg                                                                                     <  2:2079265/100‑1 (MQ=255)
                ccGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgc                                                                                    <  2:2121888/100‑1 (MQ=255)
                ccGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgc                                                                                    >  1:1468420/1‑100 (MQ=255)
                ccGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgc                                                                                    <  2:1694094/100‑1 (MQ=255)
                 cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgcg                                                                                   <  2:2717786/100‑1 (MQ=255)
                 cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgcg                                                                                   >  2:1170452/1‑100 (MQ=255)
                 cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAAcgcg                                                                                   >  2:38726/1‑100 (MQ=255)
                 cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAAcgcg                                                                                   >  1:3077126/1‑100 (MQ=255)
                   cgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGAc                                                                                 >  2:1855401/1‑100 (MQ=255)
                       aTTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTag                                                                             >  2:2233094/1‑100 (MQ=255)
                        ttCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagc                                                                            <  1:2631229/100‑1 (MQ=255)
                        ttCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagc                                                                            >  2:1546378/1‑100 (MQ=255)
                         tCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcc                                                                           <  2:2614627/100‑1 (MQ=255)
                          cTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagcca                                                                          <  1:1947564/100‑1 (MQ=255)
                          cTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTagcca                                                                          >  2:130016/1‑100 (MQ=255)
                           tGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagccag                                                                         >  1:1059877/1‑100 (MQ=255)
                            gCGTAGCGCCCTGGCGCAAGAGGCCCCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagccagc                                                                        <  2:2760257/100‑1 (MQ=255)
                             cGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagccagcc                                                                       <  2:672092/100‑1 (MQ=255)
                             cGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTagccagcc                                                                       >  1:376788/1‑100 (MQ=255)
                              gTAGCGGCCTGGCGCAAGAGGCCACGCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATAAACACGAAAAACGCGACTTAGCCAGCCg                                                                      <  1:3169759/100‑1 (MQ=255)
                              gTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCg                                                                      >  2:772324/1‑100 (MQ=255)
                               tAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGc                                                                     <  1:1220730/100‑1 (MQ=255)
                                aGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCa                                                                    >  2:2293721/1‑100 (MQ=255)
                                 gcgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCACGGTTTCGCGGGGCTGTGGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCAt                                                                   >  2:3370811/1‑100 (MQ=255)
                                 gcgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCAt                                                                   >  1:186091/1‑100 (MQ=255)
                                  cgcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCAtt                                                                  <  1:2879101/100‑1 (MQ=255)
                                   gcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTg                                                                 <  1:1095764/100‑1 (MQ=255)
                                   gcCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTg                                                                 <  1:2764417/100‑1 (MQ=255)
                                    cccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGt                                                                >  1:2851468/1‑100 (MQ=255)
                                     ccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtc                                                               >  1:1366000/1‑100 (MQ=255)
                                      cTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtct                                                              <  1:1081972/100‑1 (MQ=255)
                                      cTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtct                                                              >  2:2358481/1‑100 (MQ=255)
                                      cTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGtct                                                              >  2:1684732/1‑100 (MQ=255)
                                        ggCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTcc                                                            >  2:3065929/1‑100 (MQ=255)
                                        ggCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTcc                                                            <  1:3308038/100‑1 (MQ=255)
                                         gcgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCa                                                           <  2:2224179/100‑1 (MQ=255)
                                          cgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAc                                                          >  2:1797711/1‑100 (MQ=255)
                                          cgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAc                                                          >  2:309728/1‑100 (MQ=255)
                                          cgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAc                                                          <  2:2872003/100‑1 (MQ=255)
                                          cgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTACTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAc                                                          <  1:2214010/100‑1 (MQ=255)
                                          cgcAAGAGCCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAc                                                          <  1:3287863/100‑1 (MQ=255)
                                           gcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAcc                                                         <  1:2567480/100‑1 (MQ=255)
                                            cAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAccc                                                        >  2:1971512/1‑100 (MQ=255)
                                              agagGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAg                                                      <  2:1239272/100‑1 (MQ=255)
                                               gagGCCACCCAGCAGCGCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCAccc                                                        <  1:1445897/97‑1 (MQ=255)
                                                agGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGca                                                    <  1:2786303/100‑1 (MQ=255)
                                                agGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGACAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGca                                                    <  1:2126916/100‑1 (MQ=255)
                                                 ggCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcat                                                   >  1:1189200/1‑100 (MQ=255)
                                                   ccACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGcatca                                                 >  1:3187404/1‑100 (MQ=255)
                                                        cagcagCGCGGCACGGAAGGTTGAGCGGGGATGTCGTATCTGCATGAACACCAAACACGCGACTTAGCCAGCCGCATTGTCTCCCCCCCGca                                                    <  1:2064881/92‑1 (MQ=255)
                                                          gcagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATcaccac                                          <  1:1695591/100‑1 (MQ=255)
                                                            agcaCGGCACACAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATcaccac                                          <  1:237416/98‑1 (MQ=255)
                                                             gcacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGTACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGAc                                       <  2:483213/100‑1 (MQ=255)
                                                             gcacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGAc                                       <  2:114241/100‑1 (MQ=255)
                                                             gcacggcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCAAGCCGCATTGTCTCCACCCAGCATCATCACCACGAc                                       >  1:1132055/1‑100 (MQ=255)
                                                                  gcacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCtctc                                  <  2:2523948/100‑1 (MQ=255)
                                                                  gcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCtctc                                  <  1:2358462/100‑1 (MQ=255)
                                                                  gcacgCAAGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCtctc                                  <  1:130016/100‑1 (MQ=255)
                                                                        aaGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGc                            <  1:752765/100‑1 (MQ=255)
                                                                        aaGGTTTCGCGGTGCTGTCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGc                            <  1:2606537/100‑1 (MQ=255)
                                                                         aGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGcc                           >  2:2838220/1‑100 (MQ=255)
                                                                         aGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGcc                           >  2:2716261/1‑100 (MQ=255)
                                                                          ggTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                          >  2:925802/1‑100 (MQ=255)
                                                                          ggTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCa                          >  1:1518543/1‑100 (MQ=255)
                                                                           gTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCAc                         >  2:1521218/1‑100 (MQ=255)
                                                                           gTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCAc                         >  2:3350209/1‑100 (MQ=255)
                                                                              tCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCt                      <  1:968720/100‑1 (MQ=255)
                                                                               cgcgGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTg                     <  1:1040086/100‑1 (MQ=255)
                                                                                 cgGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCa                   <  1:3014049/100‑1 (MQ=255)
                                                                                 cgGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCa                   <  2:3313562/100‑1 (MQ=255)
                                                                                      cTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGc              <  2:3432984/100‑1 (MQ=255)
                                                                                       tGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGca             <  2:2832068/100‑1 (MQ=255)
                                                                                       tGTCGTATGCGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATTACCACGACCTCTCCGTCGCCACGCTGCATGTGc              <  1:2268242/99‑1 (MQ=255)
                                                                                        ntCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcac            >  1:1119373/2‑100 (MQ=255)
                                                                                         tCGTATGTGCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcaca           <  2:2766853/100‑1 (MQ=255)
                                                                                          cGTATGTGTATGAACACGAAAAGCGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACAt          <  1:2337582/100‑1 (MQ=255)
                                                                                          cGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGcaca           <  2:586373/99‑1 (MQ=255)
                                                                                          cGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACAt          <  2:2064333/100‑1 (MQ=255)
                                                                                            tATGTGTATGAACACG‑AAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCaa       <  1:151785/100‑1 (MQ=255)
                                                                                             aTGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGTATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCaa       >  1:1857525/1‑100 (MQ=255)
                                                                                             aTGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATcga       >  1:2100861/1‑98 (MQ=255)
                                                                                              tgtgTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAc      >  1:3418232/1‑100 (MQ=255)
                                                                                              tgtgCATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAc      >  2:1201247/1‑100 (MQ=255)
                                                                                               gtgtATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAcc     <  1:614344/100‑1 (MQ=255)
                                                                                               gtgcATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAAcc     >  1:595628/1‑100 (MQ=255)
                                                                                                tgtATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCa    >  1:1155992/1‑100 (MQ=255)
                                                                                                tgtATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCa    >  2:2667251/1‑100 (MQ=255)
                                                                                                  tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCacg  >  2:197998/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
ACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACG  >  NC_000913/3618667‑3618864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A