Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 460,326 C→T P480L (CCG→CTG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913460,3260CT98.1% 355.7 / ‑2.6 103P480L (CCG→CTG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (42/59);  total (43/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 106 total aligned reads displayed.

CAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGA  >  NC_000913/460229‑460422
                                                                                                 |                                                                                                
cAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTgc                                                                                                <  2:1280301/100‑1 (MQ=255)
 aGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTgct                                                                                               >  1:2593219/1‑100 (MQ=255)
   aaCGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACtgctgc                                                                                             <  2:2209795/100‑1 (MQ=255)
     cGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCActgctgctg                                                                                           <  1:2176020/100‑1 (MQ=255)
     cGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCActgctgctg                                                                                           <  1:696675/100‑1 (MQ=255)
      gTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTgg                                                                                          >  2:625970/1‑100 (MQ=255)
       tAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGa                                                                                         >  1:2502005/1‑100 (MQ=255)
       tAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGa                                                                                         <  1:572831/100‑1 (MQ=255)
          cGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCg                                                                                      <  1:715811/100‑1 (MQ=255)
            ttCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTa                                                                                    <  2:1380883/100‑1 (MQ=255)
            ttCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTa                                                                                    <  2:6730/100‑1 (MQ=255)
             tCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTAt                                                                                   <  2:395139/100‑1 (MQ=255)
              cAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATg                                                                                  <  1:887996/100‑1 (MQ=255)
               aGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATgg                                                                                 >  2:2509042/1‑100 (MQ=255)
                gCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGa                                                                                >  1:1743435/1‑100 (MQ=255)
                  gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGTTGCTGGATCGTATGGAAg                                                                              >  1:2537917/1‑100 (MQ=255)
                  gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAg                                                                              <  2:1827127/100‑1 (MQ=255)
                  gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAg                                                                              <  2:787323/100‑1 (MQ=255)
                  gACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAg                                                                              >  2:1621203/1‑100 (MQ=255)
                   aCCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGt                                                                             <  2:1437169/100‑1 (MQ=255)
                   aCCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGt                                                                             <  1:1637546/100‑1 (MQ=255)
                    ccACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGt                                                                             <  2:523214/99‑1 (MQ=255)
                    ccACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTg                                                                            >  2:1725040/1‑100 (MQ=255)
                      aCTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGAt                                                                          >  2:891049/1‑100 (MQ=255)
                      aCTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGAt                                                                          <  1:2410986/100‑1 (MQ=255)
                       cTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGAtt                                                                         <  2:1596719/100‑1 (MQ=255)
                        tACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTc                                                                        <  2:1775276/100‑1 (MQ=255)
                           cTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGcc                                                                     <  1:582374/100‑1 (MQ=255)
                           cTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGcc                                                                     <  1:2743157/100‑1 (MQ=255)
                           cTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGcc                                                                     >  1:2607557/1‑100 (MQ=255)
                           cTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGcc                                                                     <  1:177108/100‑1 (MQ=255)
                           cTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGcc                                                                     <  1:1074913/100‑1 (MQ=255)
                             ggAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCctc                                                                   <  2:1285988/100‑1 (MQ=255)
                                aGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTcc                                                                <  1:2551201/100‑1 (MQ=255)
                                aGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTcc                                                                <  1:1189118/100‑1 (MQ=255)
                                  tGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCgg                                                              >  2:875515/1‑100 (MQ=255)
                                   ggATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGt                                                             <  1:634301/100‑1 (MQ=255)
                                   cttccgatctctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGt                                                             <  2:518443/91‑1 (MQ=255)
                                    gATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGtt                                                            <  2:937888/100‑1 (MQ=255)
                                     aTTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTta                                                           <  1:1598420/100‑1 (MQ=255)
                                       tACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTtata                                                         >  2:159852/1‑100 (MQ=255)
                                         cGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATAcc                                                       >  2:5886/1‑100 (MQ=255)
                                         cGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATAcc                                                       <  2:1712352/100‑1 (MQ=255)
                                         cGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATAcc                                                       >  1:1798910/1‑100 (MQ=255)
                                           aTCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGa                                                     <  2:1799428/100‑1 (MQ=255)
                                           aTCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGa                                                     <  1:1812055/100‑1 (MQ=255)
                                           aTCCCAGCGACGTGATGTTTGTCGCGCCGTCGAACTCCATGAACATTGCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTgata                                                         <  2:1212764/96‑1 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGtagatcggaag                                                   >  1:518447/1‑90 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGa                                                     <  2:1693938/98‑1 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAg                                                   <  1:1734447/100‑1 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAg                                                   <  2:408726/100‑1 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAg                                                   <  2:653777/100‑1 (MQ=255)
                                             ctcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAg                                                   <  1:1109098/100‑1 (MQ=255)
                                              tcAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGa                                                  <  1:1793252/100‑1 (MQ=255)
                                               cAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGAt                                                 <  1:332056/100‑1 (MQ=255)
                                               cAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGAt                                                 >  2:2720598/1‑100 (MQ=255)
                                                    aCGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGTAAGTGATTCGCCTCTCCGGTTATACCGAAGATGaaaa                                            <  2:1486778/100‑1 (MQ=255)
                                                    aCGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGaaaa                                            <  1:1271751/100‑1 (MQ=255)
                                                    aCGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGaaa                                             >  2:1775828/1‑99 (MQ=255)
                                                     cGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGaaaaa                                           >  2:1207982/1‑100 (MQ=255)
                                                      gTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAAc                                          <  1:2082489/100‑1 (MQ=255)
                                                       tGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACt                                         >  2:2225724/1‑100 (MQ=255)
                                                        gATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTg                                        >  2:54077/1‑100 (MQ=255)
                                                        gATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTg                                        >  2:1895334/1‑100 (MQ=255)
                                                        gATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTg                                        <  1:1459467/100‑1 (MQ=255)
                                                        gATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTg                                        >  2:838731/1‑100 (MQ=255)
                                                        gATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTg                                        <  1:2747261/100‑1 (MQ=255)
                                                          tGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTAGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGaa                                      >  2:1533537/1‑100 (MQ=255)
                                                          tGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCCGGCTCGTATGGAAGTGATTCGGCTCTCCGGTTATACCGTAGTTGTAAGACTgta                                      >  2:1037996/1‑98 (MQ=255)
                                                           gTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAAc                                     <  1:1109102/100‑1 (MQ=255)
                                                           gTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAAc                                     <  1:5887/100‑1 (MQ=255)
                                                               gTCGCGACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATc                                  >  2:1527144/1‑99 (MQ=255)
                                                                 cgcgACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGcn                               <  1:841462/100‑2 (MQ=255)
                                                                 cgcgACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGcc                               >  2:1631509/1‑100 (MQ=255)
                                                                 cgcgACGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGcc                               <  1:2509061/100‑1 (MQ=255)
                                                                     aCGTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAAc                           <  1:2751773/100‑1 (MQ=255)
                                                                      ttccgatcTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACg                          <  1:2101872/93‑1 (MQ=255)
                                                                       gTCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGt                         >  1:939953/1‑100 (MQ=255)
                                                                        tCGAACTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTc                        <  2:1909313/100‑1 (MQ=255)
                                                                           aaCTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCAcc                     >  1:1407089/1‑100 (MQ=255)
                                                                           aaCTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCAcc                     <  2:1312935/100‑1 (MQ=255)
                                                                           aaCTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCAcc                     >  1:2573404/1‑100 (MQ=255)
                                                                             cTCCATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACctg                   <  2:2593200/100‑1 (MQ=255)
                                                                               ccATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACgagatcggaa                 >  2:2101855/1‑91 (MQ=255)
                                                                               ccATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACctgct                 >  1:1910860/1‑100 (MQ=255)
                                                                                cATGAACATTCCGGCACTGTTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACctgctg                <  2:2537898/100‑1 (MQ=255)
                                                                                cATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACctgctg                >  1:2326494/1‑100 (MQ=255)
                                                                                cATGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACctgctg                >  2:206874/1‑100 (MQ=255)
                                                                                  tGAACATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGcc              >  2:1033093/1‑100 (MQ=255)
                                                                                      cATTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAg          >  1:1089970/1‑100 (MQ=255)
                                                                                       aTTCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGc         <  2:289772/100‑1 (MQ=255)
                                                                                        ttCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCa        >  2:1218502/1‑100 (MQ=255)
                                                                                        ttCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCa        <  1:2357197/100‑1 (MQ=255)
                                                                                         tCCGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAg       <  2:2689990/100‑1 (MQ=255)
                                                                                           cGGCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGAt     >  1:1986411/1‑100 (MQ=255)
                                                                                            ggCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGAtt    <  2:939947/100‑1 (MQ=255)
                                                                                             gCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTg   <  2:507650/100‑1 (MQ=255)
                                                                                             gCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTg   <  1:2259161/100‑1 (MQ=255)
                                                                                             gCACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTg   <  1:1936789/100‑1 (MQ=255)
                                                                                              cACTGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGa  >  1:1474654/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGA  >  NC_000913/460229‑460422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A