Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,520,513 T→C D64G (GAT→GGT)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,520,5130TC97.9% 336.8 / ‑2.3 94D64G (GAT→GGT) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (41/51);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACC  >  NC_000913/3520417‑3520608
                                                                                                |                                                                                               
cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                              >  2:254873/1‑100 (MQ=255)
cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                              <  1:2075111/100‑1 (MQ=255)
 aGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGt                                                                                             <  1:1164117/100‑1 (MQ=255)
   gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTa                                                                                           <  2:199012/100‑1 (MQ=255)
   gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTa                                                                                           <  1:1529200/100‑1 (MQ=255)
   gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTa                                                                                           >  2:2316246/1‑100 (MQ=255)
   gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTa                                                                                           <  2:1205390/100‑1 (MQ=255)
    cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                          <  2:1802505/100‑1 (MQ=255)
    cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                          >  1:1358840/1‑100 (MQ=255)
    cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                          <  1:481127/100‑1 (MQ=255)
    cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                          >  2:1448149/1‑100 (MQ=255)
    cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                          <  2:47298/100‑1 (MQ=255)
     aaTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGa                                                                                         <  2:2132208/100‑1 (MQ=255)
     aaTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGa                                                                                         <  2:1251494/100‑1 (MQ=255)
      aTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGAt                                                                                        <  1:502579/100‑1 (MQ=255)
      aTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGATATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGAt                                                                                        >  2:1487490/1‑100 (MQ=255)
       tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATg                                                                                       <  1:1925478/100‑1 (MQ=255)
       tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATg                                                                                       <  1:1422983/100‑1 (MQ=255)
       tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATg                                                                                       <  2:2381668/100‑1 (MQ=255)
       tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCGTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATg                                                                                       <  2:2616110/100‑1 (MQ=255)
        ggAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGt                                                                                      >  1:587129/1‑100 (MQ=255)
        ggAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGt                                                                                      <  2:715179/100‑1 (MQ=255)
         gAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTa                                                                                     <  1:2492199/100‑1 (MQ=255)
         gAATGCACTGAGAGAATGTTGCCTTCCGGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTa                                                                                     <  2:830914/100‑1 (MQ=255)
           aTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAAc                                                                                   <  1:1487501/100‑1 (MQ=255)
           aTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAAc                                                                                   <  1:734043/100‑1 (MQ=255)
           aTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAAc                                                                                   <  1:2605355/100‑1 (MQ=255)
                 tGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACAcc                                                                             <  1:2498452/100‑1 (MQ=255)
                  gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCg                                                                            >  1:1337261/1‑100 (MQ=255)
                   agagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGt                                                                           <  1:1235587/100‑1 (MQ=255)
                    gagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTa                                                                          >  2:2216981/1‑100 (MQ=255)
                        aTGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGc                                                                      >  2:1349541/1‑100 (MQ=255)
                          gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTg                                                                    <  2:1315166/100‑1 (MQ=255)
                          gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTg                                                                    >  1:1033599/1‑100 (MQ=255)
                            tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                  <  1:2296448/100‑1 (MQ=255)
                            tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                  <  2:1358831/100‑1 (MQ=255)
                            tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                  >  2:1169471/1‑100 (MQ=255)
                            tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                  <  1:655352/100‑1 (MQ=255)
                            tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                  >  2:2001745/1‑100 (MQ=255)
                             gCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACg                                                                 <  1:1085734/100‑1 (MQ=255)
                              ccTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGt                                                                >  1:1191037/1‑100 (MQ=255)
                               cTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTc                                                               <  1:1706750/100‑1 (MQ=255)
                                 tCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTg                                                             <  2:2503364/100‑1 (MQ=255)
                                   cTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGc                                                           >  2:853095/1‑100 (MQ=255)
                                    tGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCg                                                          <  1:678867/100‑1 (MQ=255)
                                     gCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGa                                                         >  1:1073625/1‑100 (MQ=255)
                                        gcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGacac                                                      <  1:1359689/100‑1 (MQ=255)
                                        gcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGacac                                                      <  2:562624/100‑1 (MQ=255)
                                         cgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACg                                                     >  1:903878/1‑100 (MQ=255)
                                           ttATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGAc                                                   <  1:2028355/100‑1 (MQ=255)
                                           ttAGCAGTCCGGCTCTGGTCACTACAGTTTGTAGTGCCTGGTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGcc                                                          <  2:2623025/93‑2 (MQ=255)
                                            tATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGAcc                                                  >  2:1297267/1‑100 (MQ=255)
                                             aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCa                                                 >  2:322870/1‑100 (MQ=255)
                                             aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCa                                                 >  2:2287622/1‑100 (MQ=255)
                                             aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCa                                                 >  1:1565587/1‑100 (MQ=255)
                                              tCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAg                                                <  1:2459770/100‑1 (MQ=255)
                                               cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                               <  2:2276015/100‑1 (MQ=255)
                                                 gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                             >  2:2425912/1‑100 (MQ=255)
                                                 gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                             >  1:2151084/1‑100 (MQ=255)
                                                  tCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTc                                            <  2:1078542/100‑1 (MQ=255)
                                                  tCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTc                                            <  2:1933115/100‑1 (MQ=255)
                                                   ccGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCa                                           >  1:1356457/1‑100 (MQ=255)
                                                          ttttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTtctc                                    <  1:166486/100‑1 (MQ=255)
                                                           tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                   <  1:2192244/100‑1 (MQ=255)
                                                           tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                   <  1:1390317/100‑1 (MQ=255)
                                                            ttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCt                                  <  1:254876/100‑1 (MQ=255)
                                                              cACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                                >  1:778329/1‑100 (MQ=255)
                                                              cACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                                >  2:2464687/1‑100 (MQ=255)
                                                               aCTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGtt                               >  1:335414/1‑100 (MQ=255)
                                                                cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                              >  2:850798/1‑100 (MQ=255)
                                                                 tACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTca                             <  2:924210/100‑1 (MQ=255)
                                                                   cAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagc                           <  1:484575/100‑1 (MQ=255)
                                                                    aGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                          >  1:2246263/1‑100 (MQ=255)
                                                                     gTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                         >  1:1073441/1‑100 (MQ=255)
                                                                       ttGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                       <  1:912318/100‑1 (MQ=255)
                                                                        tggaGTGCCTGCTTCCGGGGGACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcaggc                          <  2:2464184/93‑3 (MQ=255)
                                                                        tGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcg                      >  2:1078242/1‑100 (MQ=255)
                                                                         gTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcgc                     >  2:735423/1‑100 (MQ=255)
                                                                           aGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCt                   >  2:2053898/1‑100 (MQ=255)
                                                                             tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                 <  2:1191030/100‑1 (MQ=255)
                                                                              gCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa                >  1:2108432/1‑100 (MQ=255)
                                                                              gCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa                <  2:856968/100‑1 (MQ=255)
                                                                                cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac              >  2:1726015/1‑100 (MQ=255)
                                                                                cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac              <  1:2001762/100‑1 (MQ=255)
                                                                                cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac              <  1:1448159/100‑1 (MQ=255)
                                                                                  gCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAacac            >  2:2756197/1‑100 (MQ=255)
                                                                                   cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc           <  1:2216999/100‑1 (MQ=255)
                                                                                    ttCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCt          >  1:2612823/1‑100 (MQ=255)
                                                                                     tCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTGCTGTTCAGCCAGCGCCTGCAACACCTg         >  1:1094228/1‑100 (MQ=255)
                                                                                       cAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg       <  2:2563941/100‑1 (MQ=255)
                                                                                       cAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg       >  1:1251474/1‑100 (MQ=255)
                                                                                           gAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAc   >  2:2709601/1‑100 (MQ=255)
                                                                                           gAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAc   >  2:317607/1‑100 (MQ=255)
                                                                                            aaCACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAcc  >  2:1440295/1‑100 (MQ=255)
                                                                                                |                                                                                               
CAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACC  >  NC_000913/3520417‑3520608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A