Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I0 R1 270 114.4 5840133 93.3% 5448844 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,520,513 T→C 100% D64G (GAT→GGT)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,520,5130TC92.5% 331.2 / 11.1 110D64G (GAT→GGT) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base C (47/54);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 110 total aligned reads displayed.

CCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAC  >  NC_000913/3520416‑3520607
                                                                                                 |                                                                                              
ccAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAAGATcg                                                                                              >  2:2274111/1‑99 (MQ=255)
 cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                             >  2:1640545/1‑100 (MQ=255)
 cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                             <  1:414696/100‑1 (MQ=255)
 cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                             >  2:1829272/1‑100 (MQ=255)
  aGGCAATGGAATGCACGGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGt                                                                                            <  1:640271/100‑1 (MQ=255)
   ggCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGGTATTAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGtt                                                                                           >  1:198245/1‑100 (MQ=255)
    gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTa                                                                                          <  1:1939651/100‑1 (MQ=255)
     cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                         <  1:1466697/100‑1 (MQ=255)
     cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                         >  2:1820584/1‑100 (MQ=255)
       aTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGAt                                                                                       <  1:2869800/100‑1 (MQ=255)
        tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATg                                                                                      <  2:2402546/100‑1 (MQ=255)
           aaTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTaa                                                                                   >  2:321024/1‑100 (MQ=255)
            aTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAGCTGTTAGATGTAAc                                                                                  <  2:1836687/100‑1 (MQ=255)
              gCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGa                                                                                >  1:95721/1‑100 (MQ=255)
              gCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGa                                                                                >  2:1998392/1‑100 (MQ=255)
              gCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGa                                                                                <  2:2341806/100‑1 (MQ=255)
               cACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGac                                                                               <  1:954762/100‑1 (MQ=255)
               cACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGac                                                                               >  2:1637611/1‑100 (MQ=255)
                aCTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGaca                                                                              >  2:462999/1‑100 (MQ=255)
                 cTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGacac                                                                             >  2:1092230/1‑100 (MQ=255)
                  tGAGAGAATTTTGCCTGCCTGCCGCGTTATCAGGCCGTCGCTTTTCACTACATTTTCTAGTGCCTGCTTCCAGGTAACACCTGTTAGATGTAACGACAcc                                                                            <  1:2293717/100‑1 (MQ=255)
                  tGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACAcc                                                                            <  1:1527138/100‑1 (MQ=255)
                   gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTTGATGTAACGACAcc                                                                            >  2:2870748/1‑99 (MQ=255)
                   gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCg                                                                           >  1:821046/1‑100 (MQ=255)
                   gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCg                                                                           >  2:1252460/1‑100 (MQ=255)
                   gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCg                                                                           >  1:1718926/1‑100 (MQ=255)
                   gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGACTGCTTCCAGGGAACACCTGTTAGATGTAACGCCACCg                                                                           >  2:2226845/1‑100 (MQ=255)
                     gagaATGTTGCCTTCCTGCCGCTTTATCGGTCCTGCGCTTTTCACTACAGTTTGTAGTGCCTGCGTCCAGTGAACACCTGATAGATGTAACGACACCg                                                                           <  1:2897216/98‑1 (MQ=255)
                     gagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTa                                                                         >  2:2633549/1‑100 (MQ=255)
                       gaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTAcc                                                                       <  1:1980051/100‑1 (MQ=255)
                       gaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTAcc                                                                       <  2:715099/100‑1 (MQ=255)
                       gaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTAcc                                                                       <  2:1156848/100‑1 (MQ=255)
                              gCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACg                                                                >  1:377228/1‑100 (MQ=255)
                              gCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACg                                                                <  1:467720/100‑1 (MQ=255)
                               ccTTCCTGCCGCGTTATCAGTCCTGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGt                                                               >  1:365265/1‑100 (MQ=255)
                               ccTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGt                                                               >  1:1391995/1‑100 (MQ=255)
                               ccTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGt                                                               >  1:1704571/1‑100 (MQ=255)
                                cTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGGTGACGTc                                                              <  2:1527374/100‑1 (MQ=255)
                                  tCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTg                                                            <  1:1637605/100‑1 (MQ=255)
                                    cTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGTAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGc                                                          >  1:1867355/1‑100 (MQ=255)
                                    cTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGc                                                          <  2:3332/100‑1 (MQ=255)
                                     tGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCg                                                         <  2:888293/100‑1 (MQ=255)
                                     tGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCg                                                         <  2:2436263/100‑1 (MQ=255)
                                     tGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCg                                                         >  2:2844056/1‑100 (MQ=255)
                                        cgcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                      <  1:999552/100‑1 (MQ=255)
                                             tATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGAcc                                                 >  2:1398065/1‑100 (MQ=255)
                                             tATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGAcc                                                 >  1:1485441/1‑100 (MQ=255)
                                              aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCa                                                <  2:1234863/100‑1 (MQ=255)
                                                cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                              <  2:77611/100‑1 (MQ=255)
                                                  gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                            >  2:7061/1‑100 (MQ=255)
                                                  gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAGCGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                            <  2:184929/100‑1 (MQ=255)
                                                  gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                            >  2:2762165/1‑100 (MQ=255)
                                                    ccGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACGCGACCAGGTTCa                                          <  2:1911901/100‑1 (MQ=255)
                                                     cGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCaa                                         >  1:1605577/1‑100 (MQ=255)
                                                     cGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCaa                                         >  1:2088262/1‑100 (MQ=255)
                                                       gcgcTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACt                                       <  2:55515/100‑1 (MQ=255)
                                                            tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                  >  2:1367015/1‑100 (MQ=255)
                                                            tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                  <  2:914173/100‑1 (MQ=255)
                                                            tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                  <  1:2212745/100‑1 (MQ=255)
                                                            cggcACTACAAACTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGCCACCGTCCCGCTGACGTCTGCCGACACGACCAGGATCAACTTcccc                                  >  1:791136/4‑100 (MQ=255)
                                                               cACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                               <  1:1281077/100‑1 (MQ=255)
                                                                aCTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTCCCGCTGACGTCTGGCGACACGACCAGGCTCAACTTCTCCTGt                               >  1:2736190/1‑99 (MQ=255)
                                                                 cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                             >  2:2302589/1‑100 (MQ=255)
                                                                 cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                             >  1:247687/1‑100 (MQ=255)
                                                                 cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                             <  2:803728/100‑1 (MQ=255)
                                                                 cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                             >  1:307188/1‑100 (MQ=255)
                                                                  tACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTca                            <  2:2523263/100‑1 (MQ=255)
                                                                  tACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTca                            >  1:1640467/1‑100 (MQ=255)
                                                                  tACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTca                            <  2:2705975/100‑1 (MQ=255)
                                                                   aCAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                           <  2:2435010/100‑1 (MQ=255)
                                                                   aCAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                           <  2:1573311/100‑1 (MQ=255)
                                                                   aCAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                           <  2:2581510/100‑1 (MQ=255)
                                                                     aGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                         >  2:2143206/1‑100 (MQ=255)
                                                                     aGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                         <  1:1998381/100‑1 (MQ=255)
                                                                     aGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                         >  1:2664297/1‑100 (MQ=255)
                                                                      gTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                        >  1:2522082/1‑100 (MQ=255)
                                                                        ttGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTAGCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                      <  1:2610302/100‑1 (MQ=255)
                                                                        ttGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                      >  1:1931457/1‑100 (MQ=255)
                                                                        ttGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                      >  1:1932802/1‑100 (MQ=255)
                                                                            aGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCt                  >  2:1869760/1‑100 (MQ=255)
                                                                              tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGTCAGCGCCTGc                <  2:2862799/100‑1 (MQ=255)
                                                                              tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                <  1:1544821/100‑1 (MQ=255)
                                                                               gCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa               >  2:1867670/1‑100 (MQ=255)
                                                                               gCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa               <  2:1068563/100‑1 (MQ=255)
                                                                                ccTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCaa              <  1:2239153/100‑1 (MQ=255)
                                                                                 cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac             <  1:993074/100‑1 (MQ=255)
                                                                                 cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGAAACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac             <  1:1950318/100‑1 (MQ=255)
                                                                                   gCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAacac           >  1:273334/1‑100 (MQ=255)
                                                                                    cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc          <  1:2675613/100‑1 (MQ=255)
                                                                                    cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc          >  2:2276292/1‑100 (MQ=255)
                                                                                    cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc          >  2:2053887/1‑100 (MQ=255)
                                                                                    cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc          <  1:552214/100‑1 (MQ=255)
                                                                                       ccAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGa       >  2:1355543/1‑100 (MQ=255)
                                                                                       ccAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGa       >  2:603655/1‑100 (MQ=255)
                                                                                        cAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg      <  2:198246/100‑1 (MQ=255)
                                                                                          gggAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg      <  1:2202577/98‑1 (MQ=255)
                                                                                          gggAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCt    >  2:531731/1‑100 (MQ=255)
                                                                                          gggAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCt    <  2:286740/100‑1 (MQ=255)
                                                                                           ggAACTCCTGTTACATGTAACTACACCGTACCGATGATATCGTGCGACACGACCAGGTTCAACTTCTCCTGTTCATCCAGCGCCTGCAACATCTg        <  1:850538/95‑1 (MQ=255)
                                                                                            gAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAc  <  1:1138290/100‑1 (MQ=255)
                                                                                                 |                                                                                              
CCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAC  >  NC_000913/3520416‑3520607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A