Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,021 G→A 100% A247V (GCG→GTG)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,0210GA96.9% 359.2 / 0.1 119A247V (GCG→GTG) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (51/62);  total (53/66)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 125 total aligned reads displayed.

TAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278924‑2279117
                                                                                                 |                                                                                                
tAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGcacg                                                                                                >  1:791570/1‑100 (MQ=255)
tAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACAAAAGTTTTCCGGcacg                                                                                                >  1:2563203/1‑100 (MQ=255)
 aaCGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGa                                                                                               >  1:2287220/1‑100 (MQ=255)
   cgcgCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCacgacg                                                                                             >  1:805341/1‑100 (MQ=255)
   cgcgCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCacgacg                                                                                             >  1:914471/1‑100 (MQ=255)
   cgcgCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCacgacg                                                                                             >  1:854871/1‑100 (MQ=255)
       ccAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGt                                                                                         <  2:2223693/100‑1 (MQ=255)
       ccAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGt                                                                                         <  1:2275891/100‑1 (MQ=255)
       ccAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGt                                                                                         >  1:1653134/1‑100 (MQ=255)
        cAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGtt                                                                                        <  1:2162222/100‑1 (MQ=255)
          aTGCTGCGGACGAAGCGGTTGTTAATTATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTgg                                                                                          <  1:1490766/96‑1 (MQ=255)
          aTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAACGTTTAGCGCCAAGTAGTAACCAAAATTTTCCGGCACGACGTGGTTaa                                                                                      >  2:2050301/1‑100 (MQ=255)
             cggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAAttt                                                                                   >  2:1159026/1‑100 (MQ=255)
                cggACGAAGCGGCTGTTAATGATGGTCATTACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCaa                                                                                <  1:1785548/100‑1 (MQ=255)
                cggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCaa                                                                                <  1:1741645/100‑1 (MQ=255)
                cggACGAAGCGGCTGTTAAAGATGGTCATCACGAACACAAAAACAATGTTTAGCGCAAAGTAATAACCAAATTTTTCCGGCGCGACGGGGTTAAttt                                                                                   <  2:1229253/97‑1 (MQ=255)
                 ggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCaa                                                                                <  1:404255/99‑1 (MQ=255)
                 ggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAat                                                                               <  1:2593330/100‑1 (MQ=255)
                  gACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCTCGACGTGGTTAATTTCAata                                                                              <  2:1846309/100‑1 (MQ=255)
                   aCGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAatat                                                                             >  2:1560820/1‑100 (MQ=255)
                    cGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAAtata                                                                            >  1:1528994/1‑100 (MQ=255)
                     gAAGCGGCTTTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaa                                                                           <  1:1036711/100‑1 (MQ=255)
                     gAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaa                                                                           <  2:700943/100‑1 (MQ=255)
                       aGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaac                                                                         <  1:1759247/100‑1 (MQ=255)
                        gCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGAGGTTAATTTCAATATaaaca                                                                        <  1:2637554/100‑1 (MQ=255)
                          ggCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaacaaa                                                                      >  1:2402376/1‑100 (MQ=255)
                          ggCTGTTAAAAATGGTCATCACGAACAGAAAAACAAAGTTTAACGCAAAGTAATAACCAAAGTATTCCGGCACGAAGTGCTTAATTTCAATATaaacaa                                                                       >  2:2373890/1‑99 (MQ=255)
                             tGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACg                                                                    <  2:472083/99‑1 (MQ=255)
                                tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                >  2:2014693/1‑100 (MQ=255)
                                tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                >  1:2780887/1‑100 (MQ=255)
                                tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                >  1:2561781/1‑100 (MQ=255)
                                tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                >  1:2700338/1‑100 (MQ=255)
                                  aaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCgg                                                              >  2:1529277/1‑100 (MQ=255)
                                  aaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCgg                                                              >  2:18332/1‑100 (MQ=255)
                                   aGATGGTTATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             <  2:1627116/100‑1 (MQ=255)
                                   aGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             <  1:1273641/100‑1 (MQ=255)
                                    gATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgcg                                                            >  2:2417455/1‑100 (MQ=255)
                                        gTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCAAGACGTGGTTAATAACAATATAAACAAACGGTCCGGCGCTTa                                                        >  1:1856607/1‑100 (MQ=255)
                                         tcatcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTaa                                                       >  1:1040459/1‑100 (MQ=255)
                                          catcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAg                                                      <  2:65825/100‑1 (MQ=255)
                                           atcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGa                                                     <  2:2870031/100‑1 (MQ=255)
                                            tcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGaa                                                    >  2:2161690/1‑100 (MQ=255)
                                            tcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGaa                                                    <  1:884249/100‑1 (MQ=255)
                                            tcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGaa                                                    >  2:1756742/1‑100 (MQ=255)
                                              aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  <  2:805337/100‑1 (MQ=255)
                                               cGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTAGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATga                                                 >  1:217334/1‑100 (MQ=255)
                                                gAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAATTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgag                                                <  1:341866/100‑1 (MQ=255)
                                                gAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgag                                                <  1:2018569/100‑1 (MQ=255)
                                                 aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               >  1:102161/1‑100 (MQ=255)
                                                 aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               <  2:1452524/100‑1 (MQ=255)
                                                 aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               >  1:177253/1‑100 (MQ=255)
                                                  aCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGaa                                              <  2:2512053/100‑1 (MQ=255)
                                                   cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             <  2:2857841/100‑1 (MQ=255)
                                                   cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             <  1:659114/100‑1 (MQ=255)
                                                   cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             <  2:2636325/100‑1 (MQ=255)
                                                   cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGATCCGGCGCTTAAGAATGAGAAc                                             >  2:2092633/1‑100 (MQ=255)
                                                   cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGAACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             >  2:1951618/1‑100 (MQ=255)
                                                    aGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACa                                            <  2:1736703/100‑1 (MQ=255)
                                                    aGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTACGGCGCTAAAGAATGAGAACa                                            >  1:743340/1‑100 (MQ=255)
                                                     gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  2:1091175/1‑100 (MQ=255)
                                                     gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  1:126058/1‑100 (MQ=255)
                                                     gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  2:1688777/1‑100 (MQ=255)
                                                     gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  1:323042/1‑100 (MQ=255)
                                                          aCAATGTTTAGCGCAAAGTAATAATCATCGTTTTCGGGCATTACGTGGTTAATTTCAATATAAACAAACGGGCCGGCGCTTAAGAATGAGACCATCCCgg                                      <  2:1699762/100‑1 (MQ=255)
                                                          aCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCATGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCgg                                      <  1:1357022/100‑1 (MQ=255)
                                                            aaTGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGATCCGGCGCTTAAGAATGAGAACATCCCGGCa                                    >  1:652178/1‑100 (MQ=255)
                                                               gTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAg                                 <  1:2620278/100‑1 (MQ=255)
                                                                tttAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGc                                <  1:1993064/100‑1 (MQ=255)
                                                                 ttAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACATGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGc                                >  2:1916189/1‑99 (MQ=255)
                                                                  tAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTg                              <  2:1528976/100‑1 (MQ=255)
                                                                    gcgcAAAGTAATAACCAAAGTTTTCCGGCCCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATACCGGCAAAGCTGaa                            >  1:1540565/1‑100 (MQ=255)
                                                                    gcgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaa                            >  1:311471/1‑100 (MQ=255)
                                                                     cgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaaa                           <  2:1661897/100‑1 (MQ=255)
                                                                     cgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaaa                           <  1:3029025/100‑1 (MQ=255)
                                                                     cgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaaa                           <  2:1473185/100‑1 (MQ=255)
                                                                      gcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAAc                          <  1:168113/100‑1 (MQ=255)
                                                                      gcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAAc                          >  1:1788621/1‑100 (MQ=255)
                                                                      gcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAAc                          >  1:3047015/1‑100 (MQ=255)
                                                                       cAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATAAGAACATCACGGCAAAGCTGaaa                           >  1:1716609/1‑98 (MQ=255)
                                                                        aaaGTAATTTCTAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaaa                           <  2:698335/97‑1 (MQ=255)
                                                                          aGTAATATCCAATGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGTTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACt                      <  2:1622558/100‑1 (MQ=255)
                                                                           gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  2:179502/1‑100 (MQ=255)
                                                                            taataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTgg                    <  2:645840/100‑1 (MQ=255)
                                                                              ataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCa                  <  1:400025/100‑1 (MQ=255)
                                                                               taaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCaa                 >  2:1170406/1‑100 (MQ=255)
                                                                                 aCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGc               >  2:2066838/1‑100 (MQ=255)
                                                                                 aCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAACCTGAAACCACTGGCAAg                >  2:2685690/1‑99 (MQ=255)
                                                                                  ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  1:1900517/100‑1 (MQ=255)
                                                                                  ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  2:1182060/100‑1 (MQ=255)
                                                                                  ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              >  1:1330984/1‑100 (MQ=255)
                                                                                  ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  1:1529295/100‑1 (MQ=255)
                                                                                  ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  1:2417484/100‑1 (MQ=255)
                                                                                    aaaGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATg            >  1:1179012/1‑100 (MQ=255)
                                                                                       gTTTTCCGGCACGTCGTGGTTAATTTCAATATAAACAAACGGTCCGTCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa          <  2:170493/99‑1 (MQ=255)
                                                                                       gTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGt           <  1:274437/98‑1 (MQ=255)
                                                                                        ttttCCGGTACGGCGTTTTTTATTTCAATATAAACAAACGGTCCGGCGCTTATGAATTAGAACATTCCTGTAATGTTGAAACCTCTGGCAAGCATGTAGc        <  2:2462828/100‑1 (MQ=255)
                                                                                         tttCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCt       >  1:2532028/1‑100 (MQ=255)
                                                                                           tCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCa     <  2:126057/100‑1 (MQ=255)
                                                                                            ccGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCATAGCTGAAACCACTGGCAAGCATGTAGCTCAg    <  2:485079/100‑1 (MQ=255)
                                                                                              ggCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGa  >  2:633583/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278924‑2279117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A