Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,281,279 A→T 24.9% A216A (GCA→GCT)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,2790AT24.9% 197.8 / 71.9 111A216A (GCA→GCT) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (44/37);  new base T (18/9);  total (64/47)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

Only 100 of 118 total aligned reads displayed.

GAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGC  >  NC_000913/2281181‑2281377
                                                                                                  |                                                                                                  
gAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGcac                                                                                                   <  1:2790896/100‑1 (MQ=255)
 ngCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGcaca                                                                                                  >  1:2348564/2‑100 (MQ=255)
 aGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGcaca                                                                                                  >  1:1010004/1‑100 (MQ=255)
 aGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGcaca                                                                                                  >  1:1173759/1‑100 (MQ=255)
 aGCTGCCGCTGCGTTATATGATTAAACACGGCTACCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGca                                                                                                    >  2:765981/1‑98 (MQ=255)
    tGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCagag                                                                                               <  1:1664768/100‑1 (MQ=255)
     gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGt                                                                                              >  2:3178779/1‑100 (MQ=255)
     gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGt                                                                                              >  1:2469639/1‑100 (MQ=255)
        gCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAc                                                                                           >  1:2561499/1‑100 (MQ=255)
        gCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAAAGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCTGGCACAGAGTaa                                                                                            >  2:425629/1‑99 (MQ=255)
           gCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACggg                                                                                        >  2:1716632/1‑100 (MQ=255)
           gCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACggg                                                                                        <  1:1935845/100‑1 (MQ=255)
            cGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGTCGCTTGCAGGCACAGAGTAACGGGc                                                                                       <  1:3873004/100‑1 (MQ=255)
            cGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGc                                                                                       <  1:2011355/100‑1 (MQ=255)
              ttATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTg                                                                                     <  1:3144935/100‑1 (MQ=255)
                atatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGt                                                                                    >  2:1415043/1‑99 (MQ=255)
                 tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTc                                                                                  >  1:3181777/1‑100 (MQ=255)
                  atGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCa                                                                                 >  1:2099808/1‑100 (MQ=255)
                  atGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCa                                                                                 >  2:940136/1‑100 (MQ=255)
                  atGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCa                                                                                 <  1:2982095/100‑1 (MQ=255)
                    gATTAAACACGGCTATCTGACGCCGCCAGAACGACTGCATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGc                                                                               <  2:2811604/100‑1 (MQ=255)
                     aTTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCg                                                                              >  1:4072472/1‑100 (MQ=255)
                       tAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGaa                                                                            >  1:1971075/1‑100 (MQ=255)
                        aaaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGTCTGTTCAGCGAAg                                                                           <  1:2804485/100‑1 (MQ=255)
                         aaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGc                                                                          <  1:3397960/100‑1 (MQ=255)
                          acacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGcc                                                                         <  2:1153058/100‑1 (MQ=255)
                          acacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGcc                                                                         >  2:3163446/1‑100 (MQ=255)
                           cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCg                                                                        >  1:3829682/1‑100 (MQ=255)
                           cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCg                                                                        >  1:3927434/1‑100 (MQ=255)
                           cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCg                                                                        >  2:801680/1‑100 (MQ=255)
                              ggCTATCTGACGCCGCCAGAACGACTGGATATACCAGTAGTGCAATACTATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtc                                                                     <  1:608465/100‑1 (MQ=255)
                              ggCTATCTGACGCCGCCAGAACGACTGGATAGGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtc                                                                     >  1:836165/1‑100 (MQ=255)
                               gCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtct                                                                    <  2:3109857/100‑1 (MQ=255)
                               gCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtcn                                                                    <  2:2348549/100‑2 (MQ=255)
                               gCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTGCAGCGAAGCCGAtct                                                                    >  1:4117596/1‑100 (MQ=255)
                                 tATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCCATACGATTCCACCCGCTTGCAGGCACAGAGGAACGGGCGGTTCAGCGAAGCCGATCTCa                                                                  >  2:424331/1‑100 (MQ=255)
                                 tATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCa                                                                  <  2:4072441/100‑1 (MQ=255)
                                 tATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCa                                                                  >  2:835331/1‑100 (MQ=255)
                                 tATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCa                                                                  >  1:437340/1‑100 (MQ=255)
                                   tCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAAc                                                                <  1:759364/100‑1 (MQ=255)
                                    cTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAAcc                                                               <  2:3424258/100‑1 (MQ=255)
                                     tGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCg                                                              >  1:66956/1‑100 (MQ=255)
                                       aCGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTg                                                            >  2:810531/1‑100 (MQ=255)
                                        cgccgcCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGa                                                           <  1:409399/100‑1 (MQ=255)
                                           cgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCt                                                        >  1:2075485/1‑100 (MQ=255)
                                            gccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTg                                                       >  1:1206298/1‑100 (MQ=255)
                                            gccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTg                                                       >  2:3105117/1‑100 (MQ=255)
                                            gccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTg                                                       >  1:2955083/1‑100 (MQ=255)
                                               aGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaa                                                    <  2:3830094/100‑1 (MQ=255)
                                               aGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaa                                                     <  2:450098/99‑1 (MQ=255)
                                                gAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaaa                                                   <  1:3709/100‑1 (MQ=255)
                                                 aaCGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaaaa                                                  <  1:112035/100‑1 (MQ=255)
                                                   cGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac                                                <  1:1869553/100‑1 (MQ=255)
                                                   cGACTGGATATGCCAGTAGTGCAATACGATCTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaaaca                                                 >  2:3590158/1‑97 (MQ=255)
                                                    gACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca                                               <  1:2603251/100‑1 (MQ=255)
                                                    gACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca                                               >  1:3181704/1‑100 (MQ=255)
                                                    gACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca                                               <  2:2994995/100‑1 (MQ=255)
                                                     aCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaa                                              >  1:87847/1‑100 (MQ=255)
                                                     aCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaa                                              <  2:3203486/100‑1 (MQ=255)
                                                       tGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca                                            >  2:3224594/1‑100 (MQ=255)
                                                       tGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca                                            >  2:3609177/1‑100 (MQ=255)
                                                       tGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca                                            <  1:3586340/100‑1 (MQ=255)
                                                        ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa                                           >  1:1347673/1‑100 (MQ=255)
                                                         gATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaac                                          <  1:801684/100‑1 (MQ=255)
                                                          atatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCTAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACg                                         <  2:2217755/100‑1 (MQ=255)
                                                          atatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACg                                         <  2:1173754/100‑1 (MQ=255)
                                                           tatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAATCCGATCTCAACCGTGAGCTGAAAAAAAAACAACGt                                        >  2:1817338/1‑100 (MQ=255)
                                                           tatGCCAGTAGTGCAATACGATGTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGACAAAACAACAACGt                                        >  2:2409952/1‑100 (MQ=255)
                                                            atGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGCCACAGAGCAACGGGCTGTTCAGCGAAGCCGATCTCAACAGTGAGCTGAAAAAACAACAACGTa                                       >  2:1202776/1‑100 (MQ=255)
                                                               ccAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTa                                    >  2:3115683/1‑100 (MQ=255)
                                                                 agtagtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcc                                  >  1:582506/1‑100 (MQ=255)
                                                                  gtagtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAccc                                 <  1:1622331/100‑1 (MQ=255)
                                                                   tagtGCAATACTATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                >  2:2701651/1‑100 (MQ=255)
                                                                    agtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCg                               >  1:3106551/1‑100 (MQ=255)
                                                                     gtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGc                              <  1:397155/100‑1 (MQ=255)
                                                                       gCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac                            >  2:1587734/1‑100 (MQ=255)
                                                                         tctACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat                          <  1:3028394/98‑1 (MQ=255)
                                                                          aTACGATTTCAGCCGCTAGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTCCCCCGCAcatc                         >  2:384996/1‑100 (MQ=255)
                                                                           tACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcatca                        >  1:3239242/1‑100 (MQ=255)
                                                                              gATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACAtcatca                     >  2:212502/1‑100 (MQ=255)
                                                                              gATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACAtcatca                     <  2:307382/100‑1 (MQ=255)
                                                                               aTTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAg                    >  2:3110656/1‑100 (MQ=255)
                                                                                   cAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg                >  2:2067377/1‑100 (MQ=255)
                                                                                    aGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGa               >  2:3324955/1‑100 (MQ=255)
                                                                                    aGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGa               >  1:1728756/1‑100 (MQ=255)
                                                                                     gtcGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt              >  2:3966829/3‑100 (MQ=255)
                                                                                     gCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt              >  2:517835/1‑100 (MQ=255)
                                                                                       cGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCGGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa            <  2:1658498/100‑1 (MQ=255)
                                                                                        gCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTAt           >  1:2333972/1‑100 (MQ=255)
                                                                                         cTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATg          <  1:1448225/100‑1 (MQ=255)
                                                                                          ttGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATgg         <  2:2438432/100‑1 (MQ=255)
                                                                                          ttGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATg          <  2:1558268/99‑1 (MQ=255)
                                                                                           tGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGa        <  2:2168671/100‑1 (MQ=255)
                                                                                           tGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGa        >  1:2514509/1‑100 (MQ=255)
                                                                                            gCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGAACATCATCAGCCAGATTATGGAg       <  1:835335/100‑1 (MQ=255)
                                                                                             cAGGCTCAGAGTAACGGACTGTTCAGCGAAGCCAATCTCAACCGTGAGATGAAAAAACAACAACGTATTACCCCAAACATCATAAGCCATATAAAGGa        >  2:1803916/1‑98 (MQ=255)
                                                                                             cAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg       >  2:1368647/1‑99 (MQ=255)
                                                                                             cAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGt      >  2:2100215/1‑100 (MQ=255)
                                                                                                gCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtg   >  1:3966073/1‑100 (MQ=255)
                                                                                                 cacaGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtgc  <  2:3690682/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGC  >  NC_000913/2281181‑2281377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A