Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,281,279 |
A→T |
A216A (GCA→GCT) |
yejH → |
putative ATP‑dependent DNA or RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,281,279 | 0 | A | T | 98.4%
| 214.3
/ ‑3.7
| 62 | A216A (GCA→GCT) | yejH | putative ATP‑dependent DNA or RNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (40/21); total (41/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 |
GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGT > NC_000913/2281183‑2281373
|
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag < 1:434518/100‑1 (MQ=255)
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag > 1:589037/1‑100 (MQ=255)
cTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCaga < 1:1585577/100‑1 (MQ=255)
gCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAc > 1:499113/1‑100 (MQ=255)
cTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑g > 1:101866/1‑100 (MQ=255)
tGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑gg < 1:192192/100‑1 (MQ=255)
ccgatcttatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑gg < 2:1730590/93‑1 (MQ=255)
ttATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTg < 2:202834/100‑1 (MQ=255)
tataTGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGt > 1:817774/1‑100 (MQ=255)
tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑ggagatcgg > 1:1730603/1‑93 (MQ=255)
tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTc < 1:1499566/100‑1 (MQ=255)
gATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGc < 2:541577/100‑1 (MQ=255)
aaaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAg > 2:1394717/1‑100 (MQ=255)
cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCg > 1:1745269/1‑100 (MQ=255)
cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCg > 2:29286/1‑100 (MQ=255)
cTATCTGACGCCGCCAGAACGACTGTATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGAtct < 2:1307943/99‑1 (MQ=255)
cTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGAtctc > 1:119559/1‑100 (MQ=255)
tGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCg > 1:1443226/1‑100 (MQ=255)
tGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCg < 2:499112/100‑1 (MQ=255)
gccgccAGAACGGCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGa < 2:817771/99‑1 (MQ=255)
ccgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAg > 1:1315051/1‑100 (MQ=255)
cAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaa > 2:1017472/1‑100 (MQ=255)
aGAACGCCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaa < 1:1322446/100‑1 (MQ=255)
aGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaa > 1:1349640/1‑100 (MQ=255)
cGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac > 2:615801/1‑100 (MQ=255)
ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa > 1:163052/1‑100 (MQ=255)
ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa > 2:1346752/1‑100 (MQ=255)
ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca > 1:825598/1‑99 (MQ=255)
atatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACg < 1:1909499/100‑1 (MQ=255)
tatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGt < 1:1346761/100‑1 (MQ=255)
tatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGt > 1:1683151/1‑100 (MQ=255)
atGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTa < 2:1745255/100‑1 (MQ=255)
tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt > 1:484412/1‑100 (MQ=255)
tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt > 2:588835/1‑100 (MQ=255)
gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAtt > 1:748917/1‑100 (MQ=255)
gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAtt > 1:2043321/1‑100 (MQ=255)
ccAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTa > 1:1145298/1‑100 (MQ=255)
cAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAc > 2:145959/1‑100 (MQ=255)
gtagtGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGTTCTCAACCGTGAGCTGAAAAAACAACAACGTATTAccc > 2:454853/1‑100 (MQ=255)
tGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGca > 2:1956879/1‑100 (MQ=255)
gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 1:1662590/1‑100 (MQ=255)
gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 1:448012/1‑100 (MQ=255)
gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 2:440929/1‑100 (MQ=255)
gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 2:410868/1‑100 (MQ=255)
cAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcaca > 2:1951042/1‑100 (MQ=255)
aaTACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat > 2:549568/1‑100 (MQ=255)
aaTACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat < 1:1280798/100‑1 (MQ=255)
tACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcatca > 1:1234244/1‑100 (MQ=255)
tACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcatca < 1:925589/100‑1 (MQ=255)
cGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACatcatc < 1:253866/100‑1 (MQ=255)
tttCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGc > 2:754685/1‑100 (MQ=255)
tCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCa < 1:1394726/100‑1 (MQ=255)
cAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg > 2:1181898/1‑100 (MQ=255)
cAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGAGAGCAGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg < 2:589035/100‑1 (MQ=255)
gCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACataatctggctgat > 1:1531293/1‑92 (MQ=255)
cGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa > 1:1553394/1‑100 (MQ=255)
gCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTAt > 2:680119/1‑100 (MQ=255)
cTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATg < 2:1371838/100‑1 (MQ=255)
ttGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGTTCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATgg < 1:454854/100‑1 (MQ=255)
gCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg > 2:1292498/1‑100 (MQ=255)
gCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg > 2:430414/1‑100 (MQ=255)
cAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGt > 2:1539657/1‑100 (MQ=255)
|
GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGT > NC_000913/2281183‑2281373
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
