Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,688,184	G→T	43.6%	A65E (GCG→GAG)	fumA ←	fumarate hydratase (fumarase A), aerobic Class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,688,184	0	G	T	43.6%	69.3 / 149.0	117	A65E (GCG→GAG)	fumA	fumarate hydratase (fumarase A), aerobic Class I
Reads supporting (aligned to +/- strand): ref base G (28/37); new base T (29/22); total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 124 total aligned reads displayed.

GCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAG  >  NC_000913/1688085‑1688283

gccgcGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg                                                                                                     >  2:1098496/1‑100 (MQ=255)

 ccgcGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcgc                                                                                                    >  2:354623/1‑100 (MQ=255)

  cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTcc                                                                                                   >  1:933590/1‑100 (MQ=255)

     gATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGa                                                                                                >  1:440075/1‑100 (MQ=255)

      aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGAc                                                                                               <  2:3211396/100‑1 (MQ=255)

        gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACgc                                                                                             >  1:4136622/1‑100 (MQ=255)

          cGGAGTTACGCAGGAATTGCAGCGCCACTTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgca                                                                                            <  2:1133639/99‑1 (MQ=255)

          cGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag                                                                                           <  1:1320879/100‑1 (MQ=255)

           ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagc                                                                                          <  2:2251373/100‑1 (MQ=255)

            gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACgcagca                                                                                         >  2:3221591/1‑100 (MQ=255)

            gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagca                                                                                         <  2:2446852/100‑1 (MQ=255)

              gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATg                                                                                       >  2:3073439/1‑100 (MQ=255)

              gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATg                                                                                       >  2:3814364/1‑100 (MQ=255)

              gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGTATg                                                                                       <  1:277924/100‑1 (MQ=255)

               ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATg                                                                                       >  1:2787113/1‑99 (MQ=255)

               ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGa                                                                                      <  1:3221579/100‑1 (MQ=255)

               ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGa                                                                                      <  2:1467329/100‑1 (MQ=255)

               ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGa                                                                                      <  2:1467506/100‑1 (MQ=255)

                  cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacg                                                                                   >  1:1087988/1‑100 (MQ=255)

                  cGCAGGAATTGCAGCGCAACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAAATGTTGTAGGTGCGCCGGACGCAGCATGAacg                                                                                   >  1:807853/1‑100 (MQ=255)

                   gCAGGCATTGCAGCGCCAGATATTTATCGTTTTCGCTGGCCTCAGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTACTCTGGACGCAGCATg                                                                                       >  1:3750094/1‑95 (MQ=255)

                   gCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAacga                                                                                  >  1:4277452/1‑100 (MQ=255)

                   gCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAacga                                                                                  >  1:1499101/1‑100 (MQ=255)

                   gCAGGAATTGCAGCGCCACATATTTATCATTTACGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacga                                                                                  >  2:4060551/1‑100 (MQ=255)

                     aGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg                                                                                <  1:3701611/100‑1 (MQ=255)

                       gAATTGCAGCGCCACATATTTTTCATTTTCGCTCGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca                                                                              <  1:725417/100‑1 (MQ=255)

                       gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGca                                                                              <  2:800701/100‑1 (MQ=255)

                        aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGcat                                                                             <  2:4277476/100‑1 (MQ=255)

                        aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGcat                                                                             <  2:1499105/100‑1 (MQ=255)

                           tGCAGCGCCCCATATGTATCATTTTCGCGGGCCGCCGGGTCACGCAGACTGTCGGCCACCTGTTGTTGGTGCACTGGACGCAGCATGCACGACGcatcat                                                                          <  1:4196659/100‑1 (MQ=255)

                           tGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatcat                                                                          >  2:448986/1‑100 (MQ=255)

                           tGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatcat                                                                          <  1:4197154/100‑1 (MQ=255)

                            gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATg                                                                         >  1:1778033/1‑100 (MQ=255)

                            gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATg                                                                         <  2:279905/100‑1 (MQ=255)

                            gCAGCGCCACATATTTATCAAGTTGGTTGGGCTCCGGGTCACGCCGAATGTCGGCCACATGTTGTTCATGGGCTGGACGCAAGGTCAACGACGcatc                                                                            >  1:766029/1‑97 (MQ=255)

                                cgcCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAAc                                                                     <  1:1912210/100‑1 (MQ=255)

                                  ccACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGc                                                                   >  2:2146862/1‑100 (MQ=255)

                                    acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCt                                                                 <  2:4030027/100‑1 (MQ=255)

                                    acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCt                                                                 >  2:3039423/1‑100 (MQ=255)

                                    acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCt                                                                 >  2:2792888/1‑100 (MQ=255)

                                     caTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTg                                                                >  1:2827144/1‑100 (MQ=255)

                                          ttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGgcgcg                                                           <  2:4184589/100‑1 (MQ=255)

                                          ttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGgcgcg                                                           >  1:2794875/1‑100 (MQ=255)

                                           tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCCGCATGAACGACGCATCATGAAACGCCTGgcgcgc                                                          >  1:1309913/1‑100 (MQ=255)

                                           tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGgcgcgc                                                          >  1:3979217/1‑100 (MQ=255)

                                            aTCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGcc                                                         >  2:2293139/1‑100 (MQ=255)

                                            aTCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGcc                                                         <  1:1185019/100‑1 (MQ=255)

                                             tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCa                                                        <  2:1906449/100‑1 (MQ=255)

                                             tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCa                                                        >  2:2588691/1‑100 (MQ=255)

                                                 tttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACag                                                    <  1:1994689/100‑1 (MQ=255)

                                                   tCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACagag                                                  >  2:2010735/1‑100 (MQ=255)

                                                     gCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGt                                                 >  1:3313615/1‑99 (MQ=255)

                                                        ggCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAAc                                             <  2:2399188/100‑1 (MQ=255)

                                                         gCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACg                                            <  2:3157196/100‑1 (MQ=255)

                                                          ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGc                                           >  2:1349935/1‑100 (MQ=255)

                                                             ccGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTc                                        >  1:3097127/1‑100 (MQ=255)

                                                              cGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCg                                       <  2:1038870/100‑1 (MQ=255)

                                                                ggTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCggg                                     <  1:3160013/100‑1 (MQ=255)

                                                                  tCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTg                                   <  1:3199048/100‑1 (MQ=255)

                                                                  tCACGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTg                                   <  1:137896/100‑1 (MQ=255)

                                                                   cACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGTAACGCCTGGCGCGCCAACAGAGTTTACGCTTCGGGTGc                                  <  1:2639890/100‑1 (MQ=255)

                                                                   cACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGc                                  >  2:894138/1‑100 (MQ=255)

                                                                     cGCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGa                                >  2:714555/1‑100 (MQ=255)

                                                                     cGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCACCAGAGTTAACGCTTCGGGTGCGa                                <  1:1909708/100‑1 (MQ=255)

                                                                      gCAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGAc                               <  2:3620789/100‑1 (MQ=255)

                                                                      gCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGAc                               <  1:3490353/100‑1 (MQ=255)

                                                                       cAGAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACt                              >  1:1378059/1‑100 (MQ=255)

                                                                        aGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACtt                             >  2:1622074/1‑100 (MQ=255)

                                                                         gAATGTCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACttt                            >  1:2243851/1‑100 (MQ=255)

                                                                          aaTGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTc                           >  2:3817797/1‑100 (MQ=255)

                                                                           aTGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCa                          >  1:1409219/1‑100 (MQ=255)

                                                                              tCGGCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaaa                       <  2:4276986/100‑1 (MQ=255)

                                                                              tCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaaa                       <  2:3934498/100‑1 (MQ=255)

                                                                              tCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaaa                       <  2:4043494/100‑1 (MQ=255)

                                                                                ggCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtc                     >  2:2771477/1‑100 (MQ=255)

                                                                                ggCCACCTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtc                     >  1:450737/1‑100 (MQ=255)

                                                                                ggCCACCTGTTGTTGGTGCGCCGGACGCAGCATTAACGACGCATCATGAAACGCCTTGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtc                     <  1:4256157/100‑1 (MQ=255)

                                                                                ggCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtc                     <  2:3822883/100‑1 (MQ=255)

                                                                                ggCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAt                      >  2:173336/1‑99 (MQ=255)

                                                                                ggCCACCCGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtc                     >  2:2234546/1‑100 (MQ=255)

                                                                                  ccACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAAAGATTCGGGTGCGAGTTTCAAAAtctc                   >  1:3351032/1‑100 (MQ=255)

                                                                                   cACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTcc                  <  2:793852/100‑1 (MQ=255)

                                                                                      cTGTTGTTGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGc               <  2:4031315/100‑1 (MQ=255)

                                                                                      cTGTTGTTGGTGCGCCGGACGCAGCATCAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGc               <  2:1357967/100‑1 (MQ=255)

                                                                                       tgttgtTGGTGCGCCGGACGCAGCATGATCGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGc               <  1:852758/99‑1 (MQ=255)

                                                                                        gttgttGGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGccc             <  2:157690/100‑1 (MQ=255)

                                                                                         ttgttgGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCt            <  2:1954758/100‑1 (MQ=255)

                                                                                          tgttgGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCtt           >  1:2973225/1‑100 (MQ=255)

                                                                                           gttgGTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTc          >  2:3370794/1‑100 (MQ=255)

                                                                                           gttgGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTc          >  2:1892114/1‑100 (MQ=255)

                                                                                           gttgGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTc          <  1:3768432/100‑1 (MQ=255)

                                                                                             tgGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCa         >  1:3628793/1‑99 (MQ=255)

                                                                                              ggTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCaaa       >  1:119013/1‑100 (MQ=255)

                                                                                               gTGCTCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAAt      <  2:3049994/100‑1 (MQ=255)

                                                                                               gTGCGCCGGACGCAGCATGAACGACTCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAAt      <  1:3075375/100‑1 (MQ=255)

                                                                                                tGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAAtt     >  2:279229/1‑100 (MQ=255)

                                                                                                  cgcCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCa   <  2:2464944/100‑1 (MQ=255)

                                                                                                   gcCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAg  >  1:2100293/1‑100 (MQ=255)

                                                                                                   gcCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAg  >  1:491298/1‑100 (MQ=255)

                                                                                                   gcCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAg  <  2:1261720/100‑1 (MQ=255)

GCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAG  >  NC_000913/1688085‑1688283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A