Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,751,923 T→C 42.3% G43G (GGT→GGC)  recN → recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,751,9230TC42.3% 65.8 / 164.5 149G43G (GGT→GGC) recNrecombination and repair protein
Reads supporting (aligned to +/- strand):  ref base T (33/53);  new base C (15/48);  total (48/101)
Fisher's exact test for biased strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 152 total aligned reads displayed.

TGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGC  >  NC_000913/2751824‑2752021
                                                                                                   |                                                                                                  
tGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGt                                                                                                    <  1:3923819/100‑1 (MQ=255)
  cTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCt                                                                                                  >  2:1520570/1‑100 (MQ=255)
  cTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCt                                                                                                  >  1:2022353/1‑100 (MQ=255)
  cTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCt                                                                                                  <  1:4004744/100‑1 (MQ=255)
   tATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCtt                                                                                                 <  2:843043/100‑1 (MQ=255)
   tATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCtt                                                                                                 <  1:470925/100‑1 (MQ=255)
   tATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCtt                                                                                                 <  2:3731870/100‑1 (MQ=255)
   tATCGTTCGTGAGCTTGAGATTGATTTGCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCt                                                                                                  <  1:4004794/99‑1 (MQ=255)
     tcgttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGTCGAGACCGGCTCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTg                                                                                               <  1:988325/100‑1 (MQ=255)
     tcgttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTg                                                                                               <  2:3647629/100‑1 (MQ=255)
     tcgttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTg                                                                                               <  1:3245522/100‑1 (MQ=255)
       gttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGtc                                                                                             >  2:2796938/1‑100 (MQ=255)
       gttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGtc                                                                                             <  1:3145887/100‑1 (MQ=255)
            tGAGCTTGAGATTGATTTTCTTAGCGGCATTACCGTAAGAACTAGCGACACCGGCGCGGGTAAATCTATTGCAATTGATGCCCTCGGCCTTTGTCTCggt                                                                                        <  1:692374/100‑1 (MQ=255)
            tGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCggt                                                                                        >  1:544969/1‑100 (MQ=255)
             gAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCggtg                                                                                       <  2:3155609/100‑1 (MQ=255)
              aGCTTGAGATTGATTTTCATAGCGGCATGATCTTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCggtgg                                                                                      <  1:1740566/100‑1 (MQ=255)
              aGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCggtgg                                                                                      >  1:788408/1‑100 (MQ=255)
               gCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGCTGCCCTCGGCCTTTGTCTCggttg                                                                                      >  1:4156811/1‑97 (MQ=255)
                 ttGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGGGCGGGTAAATCTATTGCAATGGATGCCCTCGGCCTTTGTCTCGGTGGTcg                                                                                   <  1:1919197/100‑1 (MQ=255)
                  tGAGATTGATTTTCATAGCGGTATGACCGTAATAACTGGCGACCCCGGCACCGGTCAATCCAGTGCAATAGATGCCCTCGGTCTTTGTCTCggtg                                                                                       <  1:2448820/95‑1 (MQ=255)
                    agaTTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTcgctc                                                                                <  2:2881677/100‑3 (MQ=255)
                    agaTTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCgcgc                                                                                <  2:3305397/100‑1 (MQ=255)
                     gattgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCt                                                                               <  2:3202694/100‑1 (MQ=255)
                     gattgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCt                                                                               >  2:2973785/1‑100 (MQ=255)
                     gattgatcTTCGTAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAAAAGATGCCCTCGGCCTTTGTCTCGGTGGTcgccgt                                                                               >  1:2248101/1‑97 (MQ=255)
                      attgaatTTCATAGGGGCATGAGAGTAATAACGTGAGAGGCCGGCGTGGGTAAATGTATTGCAATGGATGACCTCGGTCTTTGTCTCGGTGGTCgcgc                                                                                <  1:1970008/98‑1 (MQ=255)
                       ttgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTcgc                                                                                  >  1:2165507/1‑95 (MQ=255)
                        tgattTTCGTAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTAGTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTg                                                                              <  1:3569757/98‑1 (MQ=255)
                         gattTTCATAGCGGTATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAg                                                                           <  1:2749955/100‑1 (MQ=255)
                         gattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAg                                                                           >  2:1430913/1‑100 (MQ=255)
                         gattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAg                                                                           <  2:788410/100‑1 (MQ=255)
                         gattTTCATAGCGGCATGACCGTAATAACTGGCGAAACCGGCGCGGGTAAATCTAATGCCATAGATGCCCTCGGTCTTTGTATCGGTGGTAGCGCTGAAg                                                                           >  2:2488830/1‑100 (MQ=255)
                         gatgtggttAGGGGCATGACCGTTATAAATGGCGGCGGCGGCGGGGGTAGATCTAGTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCt                                                                               <  1:2075077/88‑1 (MQ=255)
                          attTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAg                                                                           <  1:3196055/99‑1 (MQ=255)
                           ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCACTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGcc                                                                         >  2:45705/1‑100 (MQ=255)
                           ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGcc                                                                         <  2:1074662/100‑1 (MQ=255)
                            tttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCg                                                                        <  2:2140000/100‑1 (MQ=255)
                               cATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACa                                                                     >  2:3666955/1‑100 (MQ=255)
                                 tAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATg                                                                   <  2:3454730/100‑1 (MQ=255)
                                  aGCTGCATTATCGTAATAACTGGAGATACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTTTCGGTGGTCGCGCCGAAGCCGACATgg                                                                  <  1:302149/100‑1 (MQ=255)
                                   gCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGt                                                                 >  2:749660/1‑100 (MQ=255)
                                    cGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTg                                                                >  2:557445/1‑100 (MQ=255)
                                      gCATGACTGTAATAACTGGCTTGACCGGCGCGGGTAATTCTATTGCATTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCg                                                                        <  1:2133930/90‑1 (MQ=255)
                                      gCATGACCGTAATAGCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCg                                                              <  1:1042664/100‑1 (MQ=255)
                                          gACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTAcc                                                          <  2:2957888/100‑1 (MQ=255)
                                            ccGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCgg                                                        <  2:4076760/100‑1 (MQ=255)
                                             cGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgc                                                       <  2:2412425/100‑1 (MQ=255)
                                              gTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgcg                                                      <  2:520772/100‑1 (MQ=255)
                                               taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgcgc                                                     <  1:2211486/100‑1 (MQ=255)
                                               taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgcgc                                                     <  2:2405406/100‑1 (MQ=255)
                                               taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgcgc                                                     >  2:2426656/1‑100 (MQ=255)
                                                       ggCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTcgcg                                             >  2:1982299/1‑100 (MQ=255)
                                                         ctagaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCGCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCt                                           <  1:368475/98‑1 (MQ=255)
                                                         cGAGGCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCt                                           <  1:1277560/100‑1 (MQ=255)
                                                         cGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCt                                           >  2:2115213/1‑100 (MQ=255)
                                                          gagaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTg                                          >  2:3396782/1‑100 (MQ=255)
                                                             aCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAcc                                       <  2:1238727/100‑1 (MQ=255)
                                                             aCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAcc                                       <  2:1608664/100‑1 (MQ=255)
                                                               cGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtg                                     <  2:1218480/100‑1 (MQ=255)
                                                                 gcgcGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgtg                                   <  2:3064204/100‑1 (MQ=255)
                                                                 gcgcGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgtg                                   <  2:500020/100‑1 (MQ=255)
                                                                 gcgcGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgtg                                   <  2:1810072/100‑1 (MQ=255)
                                                                 gcgcGGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgtg                                   <  2:1172901/100‑1 (MQ=255)
                                                                   gcgGGTAAATGTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTgcg                                 <  1:4109889/100‑1 (MQ=255)
                                                                    cgGGTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgt                                    <  1:2073025/96‑1 (MQ=255)
                                                                     gggTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGcc                               <  1:3015104/100‑1 (MQ=255)
                                                                      ggTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGccc                              >  2:540465/1‑100 (MQ=255)
                                                                      ggTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGccc                              <  1:1069689/100‑1 (MQ=255)
                                                                       gTAAATCTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCg                             <  2:1608666/100‑1 (MQ=255)
                                                                        tAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCACCAAAAAAATACGTACCGGCGCTGCCCGCGCTGAACTGTgcg                                 >  1:3756161/1‑95 (MQ=255)
                                                                        ggcaTCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt                            >  2:2010077/4‑100 (MQ=255)
                                                                          aaTCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGttt                          >  2:2518124/1‑100 (MQ=255)
                                                                           aTCTATGGCAATAGATGCCCTCGGTCTTTTTCTCGGTGGTCGCGCTCAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGttt                          <  1:162777/99‑1 (MQ=255)
                                                                             cTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                       >  2:922124/1‑100 (MQ=255)
                                                                             cTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                       <  2:525889/100‑1 (MQ=255)
                                                                             cTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                       <  1:549369/100‑1 (MQ=255)
                                                                             cTATTGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                       <  2:105480/100‑1 (MQ=255)
                                                                              tATTGTAATAGATGTCCTCGGTCTTTGTCTCTGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                       <  1:4251014/99‑1 (MQ=255)
                                                                              tATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct                      >  2:2299588/1‑100 (MQ=255)
                                                                                ttGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTctct                    <  1:2649134/100‑1 (MQ=255)
                                                                                ttGCAATAGATGCCCGCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTctct                    <  1:1815646/100‑1 (MQ=255)
                                                                                 tGCAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTg                   >  2:4124787/1‑100 (MQ=255)
                                                                                 tGCAATAGATGCCCGCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTg                   <  1:951581/100‑1 (MQ=255)
                                                                                   cAATAGATGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGaa                 <  2:756404/100‑1 (MQ=255)
                                                                                    aaTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGaaa                <  2:2790246/100‑1 (MQ=255)
                                                                                    aaTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGaaa                >  2:2179035/1‑100 (MQ=255)
                                                                                     aTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAg               <  1:2428716/100‑1 (MQ=255)
                                                                                     aTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAg               <  2:3794299/100‑1 (MQ=255)
                                                                                         aTGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATAc           <  2:2922693/100‑1 (MQ=255)
                                                                                          tGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATa            <  1:3953157/98‑1 (MQ=255)
                                                                                          tGCCCTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACg          <  1:1390425/100‑1 (MQ=255)
                                                                                            ccttCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGATCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGcc        <  1:3484445/97‑1 (MQ=255)
                                                                                            cccTCGTTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCAGTTTTTCTCTGAAAGATACGcc        <  1:333060/100‑1 (MQ=255)
                                                                                            cccTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGcc        <  2:2220145/100‑1 (MQ=255)
                                                                                            cccTCGGCCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGACAGATACGcc        >  2:3479954/1‑100 (MQ=255)
                                                                                              cTCGGCCTTTGTCTCGGTGGTCGCGGTATCATTAAAAAAGTACGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAg      >  1:1763950/1‑100 (MQ=255)
                                                                                                cGGTCTTTGTCTCGGTGGTCGCCCTGTCACTAACATAATGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCg    >  1:1714155/1‑100 (MQ=255)
                                                                                                  gTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGc  <  2:1659033/100‑1 (MQ=255)
                                                                                                  gTCGTTGTCTCTGTGGTCGCGCTGAAGCCAACATCTTGCGTCAAGGTGCGTCACGCCCCGACCGTTGCGCCCGTTTGTCTCTGAAAGATACGCCAGcctc  <  1:2997071/100‑4 (MQ=255)
                                                                                                   |                                                                                                  
TGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGC  >  NC_000913/2751824‑2752021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A