Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,594,073 G→T 51.2% T45N (ACC→AAC)  livG ← branched‑chain amino acid ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,594,0730GT51.2% 1.3 / 276.2 170T45N (ACC→AAC) livGbranched‑chain amino acid ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (34/49);  new base T (44/43);  total (78/92)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 176 total aligned reads displayed.

GCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATC  >  NC_000913/3593974‑3594171
                                                                                                   |                                                                                                  
gCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTgg                                                                                                    <  1:2344717/100‑1 (MQ=255)
gCAATTTGCTGCCCCGGTAAACCTACCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTgg                                                                                                    <  2:2165455/100‑1 (MQ=255)
 cAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGt                                                                                                   <  1:2932278/100‑1 (MQ=255)
 cAATTTGCTGCCCCGGTAAACCCTCCAGGTGGTGATCCCGCAGTAAACTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGt                                                                                                   <  1:2886471/100‑1 (MQ=255)
  aaTTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGttt                                                                                                  <  1:1187882/100‑1 (MQ=255)
   aTTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGttt                                                                                                 >  2:3850211/1‑100 (MQ=255)
        ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCg                                                                                            >  1:3803408/1‑100 (MQ=255)
        ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCg                                                                                            <  2:4235226/100‑1 (MQ=255)
          gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGc                                                                                          <  2:778866/100‑1 (MQ=255)
          gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAAGGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGc                                                                                          <  1:1675955/100‑1 (MQ=255)
           ccccGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCa                                                                                         >  2:2796667/1‑100 (MQ=255)
               ggTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGt                                                                                     <  1:2975556/100‑1 (MQ=255)
                gTAACCCTTCCAGGTGCTGAACGCGCAGTAAAATGGTGCCACCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAATAACCGTGTTTTTTCCGGc                                                                                          <  1:4173500/94‑1 (MQ=255)
                gTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGtt                                                                                    >  1:2620015/1‑100 (MQ=255)
                 tAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTa                                                                                   <  2:3255727/100‑1 (MQ=255)
                  aaaCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                  >  1:255507/1‑100 (MQ=255)
                   aaCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAACACCGTGGTTTTTCCGGCACCGTTAgg                                                                                 >  2:1850542/1‑100 (MQ=255)
                    aCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAggg                                                                                >  1:130946/1‑100 (MQ=255)
                     ccTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCCCCGTTAGGGc                                                                               >  1:2019318/1‑100 (MQ=255)
                      cTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGcc                                                                              <  1:1588760/100‑1 (MQ=255)
                         ccAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGAt                                                                           <  2:716444/100‑1 (MQ=255)
                          cAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGAtt                                                                          >  2:3957780/1‑100 (MQ=255)
                          cAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGAtt                                                                          <  2:3488859/100‑1 (MQ=255)
                           aGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAtca                                                                         >  2:2207367/1‑98 (MQ=255)
                            ggTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTaa                                                                        >  2:2551015/1‑100 (MQ=255)
                              tGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACg                                                                      >  2:348642/1‑100 (MQ=255)
                                 tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                   <  1:105557/100‑1 (MQ=255)
                                 tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                   >  1:1104001/1‑100 (MQ=255)
                                 tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                   <  1:3966570/100‑1 (MQ=255)
                                 tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgcga                                                                   >  2:533441/1‑100 (MQ=255)
                                  gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                   >  1:2442003/1‑99 (MQ=255)
                                  gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc                                                                  <  2:3540951/100‑1 (MQ=255)
                                  gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc                                                                  <  2:2921589/100‑1 (MQ=255)
                                   aTCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACg                                                                 >  2:2176161/1‑100 (MQ=255)
                                     cgcgCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAt                                                               <  1:3593152/100‑1 (MQ=255)
                                       cgatctAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtct                                                             <  2:2252844/95‑1 (MQ=255)
                                         cAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTcc                                                           >  2:1338812/1‑100 (MQ=255)
                                         cAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTcc                                                           <  1:3908152/100‑1 (MQ=255)
                                           gTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCt                                                          >  2:501/1‑99 (MQ=255)
                                           gTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTg                                                         >  2:3303440/1‑100 (MQ=255)
                                             aaaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtctagatcg                                                       >  1:2252838/1‑94 (MQ=255)
                                             aaaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCg                                                       >  1:1423757/1‑100 (MQ=255)
                                              aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCgg                                                      >  2:38465/1‑100 (MQ=255)
                                               aaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCggg                                                     <  2:405180/100‑1 (MQ=255)
                                                  ggTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTAc                                                  <  1:1665903/100‑1 (MQ=255)
                                                  ggTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTAc                                                  <  2:1453718/100‑1 (MQ=255)
                                                   gTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACa                                                 >  1:3268204/1‑100 (MQ=255)
                                                    tGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAg                                                >  1:1477198/1‑100 (MQ=255)
                                                     gccgccGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGt                                               <  2:4000943/100‑1 (MQ=255)
                                                     gccgccGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGt                                               <  1:645991/100‑1 (MQ=255)
                                                          cgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAg                                          <  2:3339981/100‑1 (MQ=255)
                                                          cgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAg                                          <  1:2727391/100‑1 (MQ=255)
                                                            gTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGAt                                        <  2:2496379/100‑1 (MQ=255)
                                                            gTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGAt                                        <  2:2532043/100‑1 (MQ=255)
                                                               ggTTTGTAGAATCCGGTCAGACAGTTAAACACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGa                                     >  1:312727/1‑100 (MQ=255)
                                                               ggTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGa                                     <  2:573933/100‑1 (MQ=255)
                                                                gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc                                    >  2:3197129/1‑100 (MQ=255)
                                                                gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTACGGCACCGTTAGAGCCGATTAACGAGGCGATCTCCTGCGGGTACAGTTCAAGATTGAc                                    >  1:1485473/1‑100 (MQ=255)
                                                                gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc                                    >  2:1175323/1‑100 (MQ=255)
                                                                 tttGTAGAGTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACg                                   <  1:1920683/100‑1 (MQ=255)
                                                                 tttGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACg                                   <  1:843161/100‑1 (MQ=255)
                                                                   tGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                 >  1:3499857/1‑100 (MQ=255)
                                                                   tGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                 <  2:1527490/100‑1 (MQ=255)
                                                                   tGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                 >  1:2371914/1‑100 (MQ=255)
                                                                     tAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt                               <  1:2061963/100‑1 (MQ=255)
                                                                       gAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTc                             <  2:2571277/100‑1 (MQ=255)
                                                                         aTCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                           >  1:912860/1‑100 (MQ=255)
                                                                         aTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                           >  2:817881/1‑100 (MQ=255)
                                                                         aTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                           <  2:1049057/100‑1 (MQ=255)
                                                                          tCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAcc                          <  1:1251156/100‑1 (MQ=255)
                                                                           ccGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCg                         <  1:4012519/100‑1 (MQ=255)
                                                                            cGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGATGATCTCCTGAGGGTACAGTTCAAGATTGACGTTGTTCACCGc                        >  1:2997682/1‑100 (MQ=255)
                                                                            cGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGc                        >  1:105343/1‑100 (MQ=255)
                                                                             tctCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGcc                       <  2:1736110/98‑1 (MQ=255)
                                                                              gTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCca                      >  2:3684056/1‑100 (MQ=255)
                                                                                cAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagc                    <  2:466872/100‑1 (MQ=255)
                                                                                 aGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca                   >  2:3048835/1‑100 (MQ=255)
                                                                                 aGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca                   <  2:126023/100‑1 (MQ=255)
                                                                                 aGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca                   <  1:1738946/100‑1 (MQ=255)
                                                                                  gACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCAGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagcag                  >  1:4258358/1‑100 (MQ=255)
                                                                                  gACAGTTAAAAACCGTGGCTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagcaa                  >  1:1983913/1‑99 (MQ=255)
                                                                                   aCAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAgg                 <  2:3089826/100‑1 (MQ=255)
                                                                                     aGTTGATAACCGTGTTTTTTCCCGAACCGTTAGGGCCGATTAACGAGATAAGCTAATTCTGGTACTGTTCAAGCTTGCTCTTGTACTCCGCCAGCAgg                 <  1:256783/98‑1 (MQ=255)
                                                                                     aGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgcc               <  1:2177710/100‑1 (MQ=255)
                                                                                      gTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTAAAGTTCAAGATTGACGTTGTTCACCGCCAGCAgg                 >  1:4163545/1‑97 (MQ=255)
                                                                                      gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg              >  2:1889468/1‑100 (MQ=255)
                                                                                      gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg              >  2:1834685/1‑100 (MQ=255)
                                                                                      cggaaaaaCCACGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg              >  2:3918082/4‑100 (MQ=255)
                                                                                       gtaagcaCCGTGGTTTTTCCGGTACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACGGTTCAAGATTGACGTTGTTCACCGCCAGCAGgc                <  1:3710835/91‑1 (MQ=255)
                                                                                        tAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccgcc            <  2:1137792/100‑1 (MQ=255)
                                                                                        tAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccgcc            >  2:2411692/1‑100 (MQ=255)
                                                                                           aaaCCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGa          <  1:2202939/99‑1 (MQ=255)
                                                                                            aaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGTTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAg        >  1:671283/1‑100 (MQ=255)
                                                                                            aaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAg        <  2:261045/100‑1 (MQ=255)
                                                                                            aaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAg        <  1:1214279/100‑1 (MQ=255)
                                                                                              ccacggTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgcg      >  2:718892/5‑100 (MQ=255)
                                                                                              ccGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACCGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgcg      >  1:1556970/1‑100 (MQ=255)
                                                                                              ccGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgcg      >  2:74514/1‑100 (MQ=255)
                                                                                                  ggTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGcatc  <  2:4130511/100‑1 (MQ=255)
                                                                                                  ggTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGcatc  >  2:1791038/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATC  >  NC_000913/3593974‑3594171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A