Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,594,073 G→T 16.6% T45N (ACC→AAC)  livG ← branched‑chain amino acid ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,594,0730GT16.6% 465.7 / 73.9 179T45N (ACC→AAC) livGbranched‑chain amino acid ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (68/81);  new base T (11/19);  total (79/100)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 182 total aligned reads displayed.

CAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCA  >  NC_000913/3593975‑3594172
                                                                                                  |                                                                                                   
cAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGt                                                                                                    <  1:1264155/100‑1 (MQ=255)
     tgctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGttttt                                                                                                <  2:2770280/99‑1 (MQ=255)
     tgctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTc                                                                                               >  2:418450/1‑100 (MQ=255)
     tgctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTc                                                                                               >  1:1828427/1‑100 (MQ=255)
      gctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTcc                                                                                              <  1:192551/100‑1 (MQ=255)
      gctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTcc                                                                                              <  1:3032426/100‑1 (MQ=255)
      gctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTcc                                                                                              >  1:2711950/1‑100 (MQ=255)
       ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCg                                                                                             <  1:102220/100‑1 (MQ=255)
       ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCg                                                                                             <  1:101546/100‑1 (MQ=255)
       ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCg                                                                                             <  1:2363899/100‑1 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTcn                                                                                              <  2:727431/98‑2 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCgg                                                                                            <  1:7752/100‑1 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCgg                                                                                            >  1:2073986/1‑100 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCg                                                                                             <  2:3857260/99‑1 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaact                                                                                            <  2:1796731/100‑5 (MQ=255)
        tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCAGTCAGACAGTTAAAAACCGTGGTTTTTCCgg                                                                                            >  2:547856/1‑100 (MQ=255)
        taccccGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCgg                                                                                            <  1:709793/98‑1 (MQ=255)
         ggcccGGTAAACCATCCAGGTGCTGATCGCGAAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGc                                                                                           >  1:2808210/3‑100 (MQ=255)
         gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAACATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGc                                                                                           >  2:3259562/1‑100 (MQ=255)
         gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGc                                                                                           >  2:264487/1‑100 (MQ=255)
         gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaactg                                                                                           <  1:1167739/100‑6 (MQ=255)
         gcCCCGGTAAACCTTCCAGGTGCTGATAGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaactg                                                                                           <  1:3887284/100‑6 (MQ=255)
          ccccGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCa                                                                                          >  1:1345462/1‑100 (MQ=255)
          ccccGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCCGCGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGCTTTTCCGGc                                                                                           >  2:1123195/1‑99 (MQ=255)
          ccccGGTAAACCGTCCAGGTGCTGTTCGGGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAACCCACGGtttttaact                                                                                            <  2:300965/98‑5 (MQ=255)
          ccccGGGAAACCTTCCAGGGGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCa                                                                                          <  1:600307/100‑1 (MQ=255)
                tAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTa                                                                                    <  1:2410531/100‑1 (MQ=255)
                 aaaCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                   >  2:457130/1‑100 (MQ=255)
                 aaaCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGGCAGCCAGTTAAAAACCGTGGTTTTTCCGGCAcc                                                                                        >  2:1636158/1‑95 (MQ=255)
                 aaaCCTTCCAGGTCCTGATCGCGCAGTAAAATGGTGCCGCCAGTGGGTTTGTAGAATCCGATCACACAGTTAAAAACCGTGGTTTTTCCGGCACCTTTAg                                                                                   >  2:2574654/1‑100 (MQ=255)
                   aCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAggg                                                                                 <  2:1283612/100‑1 (MQ=255)
                    ccTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAggg                                                                                 <  1:409363/99‑1 (MQ=255)
                    ccTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGc                                                                                <  2:2300989/100‑1 (MQ=255)
                     gatCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGGATCCGGTCAGAAAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                   >  2:240737/3‑96 (MQ=255)
                      ttCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCg                                                                              <  2:1740146/100‑1 (MQ=255)
                       tCCGGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAAACCGGTCAGACAGGTAAAAACCGTGGTTTTTCCGGCGCCGTTAAGGc                                                                                <  2:3209213/97‑1 (MQ=255)
                         cAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAtt                                                                           <  1:4160348/100‑1 (MQ=255)
                         cAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAtt                                                                           >  1:3321343/1‑100 (MQ=255)
                          aGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGCGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTa                                                                          <  1:2932034/100‑1 (MQ=255)
                             tGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACg                                                                       <  1:3029282/100‑1 (MQ=255)
                             tGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACg                                                                       <  1:1753016/100‑1 (MQ=255)
                              gCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACga                                                                      <  2:51830/100‑1 (MQ=255)
                               cTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgag                                                                     <  1:2481117/100‑1 (MQ=255)
                               cTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgag                                                                     <  1:3448244/100‑1 (MQ=255)
                                tgctCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                    >  2:2854991/4‑100 (MQ=255)
                                tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACGGTTAGGGCCGATTAACgaga                                                                    >  2:3099255/1‑100 (MQ=255)
                                tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                    >  2:905528/1‑100 (MQ=255)
                                tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                    >  1:2405382/1‑100 (MQ=255)
                                tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgaga                                                                    >  1:2155770/1‑100 (MQ=255)
                                 gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc                                                                   >  1:4173351/1‑100 (MQ=255)
                                  aTCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACg                                                                  >  2:731433/1‑100 (MQ=255)
                                  aTCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACg                                                                  <  2:3415172/100‑1 (MQ=255)
                                    cgcgCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAt                                                                >  1:1505946/1‑100 (MQ=255)
                                     gcgcAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtc                                                               <  1:457133/100‑1 (MQ=255)
                                         aGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCt                                                           <  2:1673565/100‑1 (MQ=255)
                                         aGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCt                                                           >  1:1336287/1‑100 (MQ=255)
                                             aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCgg                                                       <  2:540362/100‑1 (MQ=255)
                                             aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCgg                                                       >  2:3895088/1‑100 (MQ=255)
                                              aaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTAAGACAGTTAAAAACCGCGGTTTTTCCGGCACCGCTAGGGCCGATTAGCGAGACGCTCTGCTGCgg                                                       >  2:1184419/1‑99 (MQ=255)
                                                tGGTGCCGCCGGTGGGATTCTAGAATACGGTCAGACAGTTAAAAACCGTTGTTTATCCGGCAACGATAGGGACGAATAACGAGACGATCCCCGGCgcg                                                      >  2:390361/1‑96 (MQ=255)
                                                 ggTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTAc                                                   <  2:182624/100‑1 (MQ=255)
                                                  gTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACa                                                  <  2:3552297/100‑1 (MQ=255)
                                                   tGCCGCCGGTGTGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAg                                                 <  2:360516/100‑1 (MQ=255)
                                                   tGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGTCCGATTAACGAGACGATCTCCTGCGGGTACAg                                                 >  2:3326458/1‑100 (MQ=255)
                                                       gccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGGACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGtt                                               <  2:1216873/98‑1 (MQ=255)
                                                       gccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCa                                             <  1:223085/100‑1 (MQ=255)
                                                       gccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCa                                             >  1:1695581/1‑100 (MQ=255)
                                                        ccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                            <  2:1941295/100‑1 (MQ=255)
                                                        ccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                            <  1:3580399/100‑1 (MQ=255)
                                                          ggTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAGCGAGACGATCTCCTGCGGGTACAGTTCAAg                                           <  2:2072520/99‑1 (MQ=255)
                                                          ggTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGa                                          <  1:1578077/100‑1 (MQ=255)
                                                             gggTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTg                                       <  2:567466/100‑1 (MQ=255)
                                                             gggTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTg                                       <  2:3268208/100‑1 (MQ=255)
                                                               gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc                                     >  1:3226110/1‑100 (MQ=255)
                                                               gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc                                     >  1:836790/1‑100 (MQ=255)
                                                                tttGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTGCCGGCACCGTTAGGGTCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACg                                    <  2:1428524/100‑1 (MQ=255)
                                                                  tGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                  >  1:2669984/1‑100 (MQ=255)
                                                                   gTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttg                                 <  1:2027740/100‑1 (MQ=255)
                                                                      gAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTc                              <  2:2692333/100‑1 (MQ=255)
                                                                      gAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTc                              <  1:2459661/100‑1 (MQ=255)
                                                                       aaTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCa                             <  2:1920796/100‑1 (MQ=255)
                                                                        aTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                            >  1:3595724/1‑100 (MQ=255)
                                                                        aTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                            >  1:1368692/1‑100 (MQ=255)
                                                                         tCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAcc                           <  1:340043/100‑1 (MQ=255)
                                                                         tCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAcc                           >  2:3977424/1‑100 (MQ=255)
                                                                          ccGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCaaga                          >  2:2235330/1‑97 (MQ=255)
                                                                           cGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGc                         >  1:3139332/1‑100 (MQ=255)
                                                                           cGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGc                         >  1:687593/1‑100 (MQ=255)
                                                                            ggTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGcc                        <  2:2779250/100‑1 (MQ=255)
                                                                            ggTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGcc                        >  1:545489/1‑100 (MQ=255)
                                                                             gTCAGACAGTTAAACACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTCCAGTTCAAGATTGACGTTGTTCACCGCca                       <  2:2669968/100‑1 (MQ=255)
                                                                               cAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagc                     <  1:3067378/100‑1 (MQ=255)
                                                                               cAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagc                     <  2:3065368/100‑1 (MQ=255)
                                                                               cAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagc                     >  1:1405292/1‑100 (MQ=255)
                                                                                  aCAGTGAAGAGCCGTGGTTTTGCCGGTACCGTTAGGGCCGATTAGCGAAACGATCTCCTGCTGGTACAGTTCAACATTGACGCTGTTCACCGCcagc                     <  2:2479262/97‑1 (MQ=255)
                                                                                   gccggaaaaaCCACGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgc                 >  1:2186254/6‑100 (MQ=255)
                                                                                   cAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgc                 <  1:3860845/100‑1 (MQ=255)
                                                                                     gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg               >  1:2332702/1‑100 (MQ=255)
                                                                                     gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg               >  1:1154502/1‑100 (MQ=255)
                                                                                      ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccgc              <  2:100203/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCA  >  NC_000913/3593975‑3594172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A