Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,795,591 G→A 58.6% S221S (TCG→TCA)  waaF → ADP‑heptose:LPS heptosyltransferase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,795,5910GA58.6% 93.2 / 203.1 179S221S (TCG→TCA) waaFADP‑heptose:LPS heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base G (30/43);  new base A (53/50);  total (85/94)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAT  >  NC_000913/3795495‑3795687
                                                                                                |                                                                                                 
ggAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCg                                                                                                >  2:3833166/1‑100 (MQ=255)
   gTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGaaa                                                                                             <  2:2059429/100‑1 (MQ=255)
   gTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGaaa                                                                                             <  1:2411240/100‑1 (MQ=255)
   gTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGaaa                                                                                             >  2:2329708/1‑100 (MQ=255)
     tttgTCCTGCAACACGCTGGCCACACGACCACTATGCGGAGCTGGCAAAGCAGCTGATTACTTAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAg                                                                                            <  2:241481/96‑1 (MQ=255)
      tGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGAt                                                                                          <  1:3606689/100‑1 (MQ=255)
       ggTCCTGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATc                                                                                         >  1:3839818/1‑100 (MQ=255)
       ggTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATc                                                                                         >  2:1057051/1‑100 (MQ=255)
        gTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCa                                                                                        <  1:263677/100‑1 (MQ=255)
        gTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCCGCGAAAGATCa                                                                                        >  2:1963019/1‑100 (MQ=255)
         tCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCa                                                                                        <  1:3929595/99‑1 (MQ=255)
         tCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCAt                                                                                       <  1:65278/100‑1 (MQ=255)
          ccGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATg                                                                                      >  1:3085348/1‑100 (MQ=255)
           cGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGa                                                                                     <  2:3339607/100‑1 (MQ=255)
               aaaaCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCg                                                                                 <  1:1653066/100‑1 (MQ=255)
                aaaCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCgg                                                                                <  2:556530/100‑1 (MQ=255)
                  aCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGc                                                                              >  2:3923983/1‑100 (MQ=255)
                    gCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCaa                                                                            >  1:1223665/1‑100 (MQ=255)
                     cTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAAt                                                                           >  1:3811424/1‑100 (MQ=255)
                       ggCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATga                                                                         >  1:1657152/1‑100 (MQ=255)
                       ggCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATga                                                                         >  1:2018011/1‑100 (MQ=255)
                       ggCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATga                                                                         >  1:3142166/1‑100 (MQ=255)
                       ggCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATga                                                                         <  1:606257/100‑1 (MQ=255)
                         ccACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATgaga                                                                       >  2:5248/1‑100 (MQ=255)
                         ccACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATgaga                                                                       >  2:3586722/1‑100 (MQ=255)
                          cacaCTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGAt                                                                      <  1:2786335/100‑1 (MQ=255)
                          cacaCTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGAt                                                                      <  1:2940472/100‑1 (MQ=255)
                           acacTACCACTATGCGGAGCTGGCAAAGGAGCTGATTGATGAAGGTTATCAGGTGGTTCGGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGAtt                                                                     <  1:1243057/100‑1 (MQ=255)
                            cacTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTc                                                                    <  2:2316795/100‑1 (MQ=255)
                             acTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGAt                                                                      <  1:3338611/97‑1 (MQ=255)
                               tACCACTATGCGGAGCTGGCAAAGCAGCTTATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGTCGAAAGCTCATGAAGCGGGCAATGAGATTCttt                                                                 <  1:2571390/100‑2 (MQ=255)
                               tACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTg                                                                 >  1:1160740/1‑100 (MQ=255)
                                aCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGc                                                                >  1:2079391/1‑100 (MQ=255)
                                aCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGc                                                                <  2:3320994/100‑1 (MQ=255)
                                   aCTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGc                                                             >  2:1256933/1‑100 (MQ=255)
                                      aTGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCttt                                                          <  2:3966511/100‑1 (MQ=255)
                                       tGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTg                                                         <  1:926749/100‑1 (MQ=255)
                                       tGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCtttn                                                         <  1:3720714/100‑2 (MQ=255)
                                       tGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTg                                                         <  1:3449125/100‑1 (MQ=255)
                                         cGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGaa                                                       <  2:1192689/100‑1 (MQ=255)
                                          gaaGCTGGCAAAGCAGCTGATTGAGGAAGGTTATCAGGTGGTTCTGTTTGTCTCAGCGAAAGATCTTGAAGCGGGCAATGAGATGCTTGCCGCTTTGAAt                                                      <  1:1335684/98‑1 (MQ=255)
                                            aGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATAc                                                    <  1:1554875/100‑1 (MQ=255)
                                            aGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATAc                                                    >  2:2990153/1‑100 (MQ=255)
                                              cTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCg                                                  <  2:284683/100‑1 (MQ=255)
                                              cTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCg                                                  <  2:2794819/100‑1 (MQ=255)
                                               tGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGa                                                 >  1:3193809/1‑100 (MQ=255)
                                                ggCCAAGCAGCTGATTGATGAACGTTATCATGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGTGATTCTTGCCGCTCTGAATACCGag                                                >  1:170540/1‑100 (MQ=255)
                                                ggCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGag                                                >  2:292765/1‑100 (MQ=255)
                                                ggCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGag                                                >  2:1038635/1‑100 (MQ=255)
                                                ggCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGag                                                >  2:4112035/1‑100 (MQ=255)
                                                ggCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGGTCTGTGTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGag                                                <  1:3575823/100‑1 (MQ=255)
                                                 gCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGagc                                               <  2:2077553/100‑1 (MQ=255)
                                                  cAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGagca                                              >  2:731597/1‑100 (MQ=255)
                                                  cAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGagca                                              <  1:822969/100‑1 (MQ=255)
                                                  cAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGagca                                              <  2:1560895/100‑1 (MQ=255)
                                                     agcagcTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAgcagca                                           <  2:3626476/100‑1 (MQ=255)
                                                      gcagcTGATTGGTGAAGGTTATCAGGTGGTTCGGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAgcagca                                           >  1:2866251/1‑99 (MQ=255)
                                                           tgattgatGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATg                                     >  2:2544840/1‑100 (MQ=255)
                                                            nattgatGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATgg                                    >  1:3880511/2‑100 (MQ=255)
                                                             attgatGAAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGgt                                   >  1:1243141/1‑100 (MQ=255)
                                                               tgatgaAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGgtgt                                 <  2:808003/100‑1 (MQ=255)
                                                                gatgaAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAAGCATGGTGTc                                <  1:2343405/100‑1 (MQ=255)
                                                                gatgaAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTc                                >  1:4226987/1‑100 (MQ=255)
                                                                gatgaAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTc                                <  2:2159917/100‑1 (MQ=255)
                                                                gatgaAGGTTATCAGGTGGTTCTGTTTGGCTCAGCCAAAGATGATGAAGCGGGCAATGAAATTCTTGCCGCTATGAATACCGAGCTGCAGGCAtgttgt                                 >  1:3763211/1‑99 (MQ=255)
                                                                 atgaAGGTTAGCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCg                               <  1:2790615/100‑1 (MQ=255)
                                                                  tgaAGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATGATGAGGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCgg                              <  1:1848276/100‑1 (MQ=255)
                                                                   gaAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGa                             >  2:3544384/1‑100 (MQ=255)
                                                                    aaGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGaa                            <  2:373355/100‑1 (MQ=255)
                                                                    aaGGTTATCAGGTGGGTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGGGATTCTGGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGaa                            <  1:776230/100‑1 (MQ=255)
                                                                     aGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAAc                           >  1:561176/1‑100 (MQ=255)
                                                                     aGGTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAAc                           >  1:2028078/1‑100 (MQ=255)
                                                                      ggTTATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAAcc                          >  2:4035074/1‑100 (MQ=255)
                                                                        ttATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTg                        <  1:1070608/100‑1 (MQ=255)
                                                                         tATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTgg                       <  1:1468375/100‑1 (MQ=255)
                                                                         tATCAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTgg                       <  1:1963015/100‑1 (MQ=255)
                                                                            cAGGTGGTTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑gg                   >  2:3927953/1‑100 (MQ=255)
                                                                             aGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑ggg                  >  2:325625/1‑100 (MQ=255)
                                                                              ggtggtTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑gggg                 >  2:1491429/1‑100 (MQ=255)
                                                                              ggtggtTCTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑gggg                 <  2:2843430/100‑1 (MQ=255)
                                                                               gtggtTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑ggggg                <  2:1002800/100‑1 (MQ=255)
                                                                                tggtTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGa               >  1:2041126/1‑100 (MQ=255)
                                                                                tggtTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGa               <  2:1722177/100‑1 (MQ=255)
                                                                                   ttCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAac            <  2:919521/100‑1 (MQ=255)
                                                                                   ttCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAac            <  1:797749/100‑1 (MQ=255)
                                                                                   ttCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAac            <  1:738962/100‑1 (MQ=255)
                                                                                     cTGTTTGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAacac          >  2:2016737/1‑100 (MQ=255)
                                                                                      tGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAAcaca         >  1:2691045/1‑100 (MQ=255)
                                                                                         ttGGCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCt      <  2:3472213/100‑1 (MQ=255)
                                                                                           ggCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTg    >  1:3527665/1‑100 (MQ=255)
                                                                                           ggCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTg    >  2:3302626/1‑100 (MQ=255)
                                                                                           ggCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTg    >  1:2242932/1‑100 (MQ=255)
                                                                                           ggCTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTg    >  1:105586/1‑100 (MQ=255)
                                                                                            gCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGGAAACACAGCTTg    <  2:1363317/100‑1 (MQ=255)
                                                                                            gCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGa   <  2:1612683/100‑1 (MQ=255)
                                                                                             cTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAt  >  2:1438408/1‑100 (MQ=255)
                                                                                             cTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAt  >  1:3016326/1‑100 (MQ=255)
                                                                                             cTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAt  >  2:3372578/1‑100 (MQ=255)
                                                                                             cTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAt  >  2:2751358/1‑100 (MQ=255)
                                                                                             cTCAGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAt  >  1:1032704/1‑100 (MQ=255)
                                                                                                |                                                                                                 
GGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGC‑GGGGGAAACACAGCTTGAT  >  NC_000913/3795495‑3795687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A