Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,370,670 C→A 50.3% intergenic (‑258/‑18) yjeH ← / → groS putative transporter/Cpn10 chaperonin GroES, small subunit of GroESL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,370,6700CA50.3% 9.3 / 284.1 171intergenic (‑258/‑18)yjeH/groSputative transporter/Cpn10 chaperonin GroES, small subunit of GroESL
Reads supporting (aligned to +/- strand):  ref base C (43/41);  new base A (46/39);  total (89/82)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 173 total aligned reads displayed.

TCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTGA  >  NC_000913/4370574‑4370769
                                                                                                |                                                                                                   
 gccccTTCAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCaa                                                                                                 >  1:3220564/2‑100 (MQ=255)
 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATTACAGATACGGACTTTATCaa                                                                                                 <  1:2607289/100‑1 (MQ=255)
 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCaa                                                                                                 >  2:1778951/1‑100 (MQ=255)
 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCa                                                                                                  <  1:3766575/99‑1 (MQ=255)
  ccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaaa                                                                                                >  2:78697/1‑100 (MQ=255)
  ccccTTGAAGGGGCGAAGCCTCATCCCCATCTCACTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGGCTTTCTCaaa                                                                                                >  1:2804633/1‑100 (MQ=255)
       tGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGgaga                                                                                           >  2:2097431/1‑100 (MQ=255)
       tGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGgaga                                                                                           >  2:3516197/1‑100 (MQ=255)
        tacgggtCGAAGCCTCATCACCATTTCTCTGATCACCAGCCGGCAAACCACGTAACCACCGCAGTCACCCATAACAGCTACGGACTTTATCAAAGgag                                                                                            >  1:1271463/4‑98 (MQ=255)
        gAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGagag                                                                                          <  1:1808561/100‑1 (MQ=255)
          aGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGACACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGtt                                                                                        <  1:2732088/100‑1 (MQ=255)
          aGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAATCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGtt                                                                                        <  1:2375209/100‑1 (MQ=255)
           gcggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTa                                                                                       <  1:2588236/98‑1 (MQ=255)
             ggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                                                                                     >  1:3195401/1‑100 (MQ=255)
             ggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                                                                                     <  2:4049490/100‑1 (MQ=255)
             ggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATc                                                                                     >  2:31799/1‑100 (MQ=255)
             ggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATc                                                                                     >  2:215353/1‑100 (MQ=255)
             ggCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCAATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                                                                                     >  2:150588/1‑100 (MQ=255)
               cGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCaa                                                                                   <  2:2431601/100‑1 (MQ=255)
                gAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAAt                                                                                  >  2:1528494/1‑100 (MQ=255)
                gAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAAt                                                                                  <  2:2208107/100‑1 (MQ=255)
                  aGCCTCATCCCTATTTCTCTGGTCATCAGCCGGTAAACCACGTAAGCTCCGGTGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAAt                                                                                  <  1:1579801/98‑1 (MQ=255)
                    ccTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAat                                                                              >  2:4155931/1‑100 (MQ=255)
                     cTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAata                                                                             >  1:216681/1‑100 (MQ=255)
                     cTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAata                                                                             >  2:489184/1‑100 (MQ=255)
                     cTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTACCAATGAata                                                                             >  2:2748105/1‑100 (MQ=255)
                       cATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATAtt                                                                           >  2:3694925/1‑100 (MQ=255)
                         tCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCg                                                                         <  2:2588952/100‑1 (MQ=255)
                           cccATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTc                                                                       <  1:932950/100‑1 (MQ=255)
                            ccATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTcc                                                                      >  1:4100852/1‑100 (MQ=255)
                            ccATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTc                                                                       <  1:1467148/99‑1 (MQ=255)
                             cATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCa                                                                     >  2:3277492/1‑100 (MQ=255)
                             cATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCa                                                                     <  2:3614232/100‑1 (MQ=255)
                              aTTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACATATACGGACTTTCTCAAAGGAGAGTTAACAATGAATATTCGTCCAt                                                                    >  2:589731/1‑100 (MQ=255)
                              aTTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCAt                                                                    >  2:2841927/1‑100 (MQ=255)
                                 tctcTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGc                                                                 >  1:1993812/1‑100 (MQ=255)
                                  ctctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCa                                                                <  1:3663699/100‑1 (MQ=255)
                                  ctctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCa                                                                >  2:2534578/1‑100 (MQ=255)
                                    ctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATg                                                              >  1:3714974/1‑100 (MQ=255)
                                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAt                                                            >  2:3986725/1‑100 (MQ=255)
                                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAt                                                            >  2:3557227/1‑100 (MQ=255)
                                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAt                                                            >  1:779217/1‑100 (MQ=255)
                                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAt                                                            >  1:1062837/1‑100 (MQ=255)
                                       gTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATc                                                           <  2:2069055/100‑1 (MQ=255)
                                       gTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATc                                                           >  1:1717219/1‑100 (MQ=255)
                                          aCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATcgcg                                                        <  2:854179/100‑1 (MQ=255)
                                          aCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATcgcg                                                        <  1:3870006/100‑1 (MQ=255)
                                           ccAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGt                                                       >  1:2035096/1‑100 (MQ=255)
                                           ccAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGt                                                       >  2:2970391/1‑100 (MQ=255)
                                           ccAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGt                                                       >  2:2686830/1‑100 (MQ=255)
                                            cAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTg                                                      >  2:1131051/1‑100 (MQ=255)
                                            cAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTg                                                      >  1:267143/1‑100 (MQ=255)
                                            cAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTg                                                      >  2:2855992/1‑100 (MQ=255)
                                             aGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGa                                                     >  1:3434582/1‑100 (MQ=255)
                                              gCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGAt                                                    <  2:52698/100‑1 (MQ=255)
                                               ccGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGCCTTTAACAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATc                                                   >  2:1190810/1‑100 (MQ=255)
                                                cGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCg                                                  <  2:782224/100‑1 (MQ=255)
                                                cGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTCATCAATGAATATTCGTCCATTGCATGATCGCGTGATCg                                                  <  1:2627270/100‑1 (MQ=255)
                                                  ggAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTc                                                <  1:1137815/100‑1 (MQ=255)
                                                  ggAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTc                                                >  1:2841010/1‑100 (MQ=255)
                                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAg                                             <  1:580795/100‑1 (MQ=255)
                                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAg                                             <  2:3693212/100‑1 (MQ=255)
                                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAg                                             >  2:1649523/1‑100 (MQ=255)
                                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAg                                             <  1:31799/100‑1 (MQ=255)
                                                      aCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGc                                            >  2:1786008/1‑100 (MQ=255)
                                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           <  2:1320352/100‑1 (MQ=255)
                                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           <  1:3516184/100‑1 (MQ=255)
                                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           <  1:3504422/100‑1 (MQ=255)
                                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           >  1:473550/1‑100 (MQ=255)
                                                        cACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGt                                          <  2:3446405/100‑1 (MQ=255)
                                                        cACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGt                                          >  2:4125435/1‑100 (MQ=255)
                                                            tAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAaag                                      >  2:2197664/1‑100 (MQ=255)
                                                                cTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGt                                  <  2:3397900/100‑1 (MQ=255)
                                                                  ccGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTg                                <  2:809935/100‑1 (MQ=255)
                                                                   cGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTg                                <  2:1810588/99‑1 (MQ=255)
                                                                   cGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGa                               <  2:3063990/100‑1 (MQ=255)
                                                                      cGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAAc                            <  2:1834398/100‑1 (MQ=255)
                                                                         cACCCATATCAGATACGGACTTTTTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACt                           <  2:152260/98‑1 (MQ=255)
                                                                          aCCCATAACAGATGCGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaaa                        <  1:181524/100‑1 (MQ=255)
                                                                          aCCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaaa                        >  2:649012/1‑100 (MQ=255)
                                                                           cccATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAAt                       >  1:3864970/1‑100 (MQ=255)
                                                                           cccATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAAt                       >  1:1934810/1‑100 (MQ=255)
                                                                            ccATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      >  1:3610918/1‑100 (MQ=255)
                                                                            ccATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      >  1:3105005/1‑100 (MQ=255)
                                                                             cATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATct                     >  1:4216459/1‑100 (MQ=255)
                                                                              aTAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctg                    <  2:3391209/100‑1 (MQ=255)
                                                                              aTAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctg                    >  2:3204415/1‑100 (MQ=255)
                                                                                 aCAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgctg                 >  2:608393/1‑100 (MQ=255)
                                                                                  cAGATACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTgg                >  2:626121/1‑100 (MQ=255)
                                                                                     aTACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTggcgg             >  1:2985386/1‑100 (MQ=255)
                                                                                      tACGGACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTggcggc            >  1:2048287/1‑100 (MQ=255)
                                                                                       aCGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCa           <  2:1393207/100‑1 (MQ=255)
                                                                                         ggACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATc         >  2:2340256/1‑100 (MQ=255)
                                                                                         ggACTTTATCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATc         >  1:879531/1‑100 (MQ=255)
                                                                                          gACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATc         <  1:1514008/99‑1 (MQ=255)
                                                                                          gACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCg        >  1:460939/1‑100 (MQ=255)
                                                                                           aCTTTGTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGt       <  1:4080682/100‑1 (MQ=255)
                                                                                            cTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGtt      >  1:2439247/1‑100 (MQ=255)
                                                                                             tttCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTc     <  2:336667/100‑1 (MQ=255)
                                                                                                ctcAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTGa  >  2:1579558/1‑100 (MQ=255)
                                                                                                |                                                                                                   
TCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTGA  >  NC_000913/4370574‑4370769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A