Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,386,365 T→A 76.5% F169Y (TTC→TAC)  yfbL → putative M28A family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,3650TA76.5% 257.3 / 81.9 132F169Y (TTC→TAC) yfbLputative M28A family peptidase
Reads supporting (aligned to +/- strand):  ref base T (17/14);  new base A (54/46);  total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 134 total aligned reads displayed.

TGTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTC  >  NC_000913/2386270‑2386464
                                                                                               |                                                                                                   
tgtgGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACttt                                                                                                 <  2:849263/100‑1 (MQ=255)
 gtgGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTc                                                                                                <  1:2318223/100‑1 (MQ=255)
  tgGCAGTATTACTCGAACTGGCACTTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCCGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCg                                                                                               <  2:2385415/100‑1 (MQ=255)
   ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTCCTTTCGt                                                                                              >  1:1645164/1‑100 (MQ=255)
   ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGt                                                                                              >  2:3120532/1‑100 (MQ=255)
      aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTTGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                           <  1:668648/100‑1 (MQ=255)
      aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGc                                                                                           <  2:3038435/100‑1 (MQ=255)
       ggATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCg                                                                                          <  1:1659593/100‑1 (MQ=255)
         aTTACTCGAACTGGCGCGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTGCGTAGCGAt                                                                                        <  1:2804620/100‑1 (MQ=255)
             cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGaaa                                                                                    >  2:59313/1‑100 (MQ=255)
             cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGaaa                                                                                    >  1:2012092/1‑100 (MQ=255)
               cGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATg                                                                                  >  1:1135908/1‑100 (MQ=255)
                   cTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGa                                                                              <  2:1008976/100‑1 (MQ=255)
                    tGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAg                                                                             <  2:1507642/100‑1 (MQ=255)
                     ggCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgc                                                                            >  2:2844803/1‑100 (MQ=255)
                     ggCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgc                                                                            >  1:708351/1‑100 (MQ=255)
                       cACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgcgc                                                                          >  2:2189204/1‑100 (MQ=255)
                          gTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGt                                                                       >  2:1009762/1‑100 (MQ=255)
                           tttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtg                                                                      <  1:1047653/100‑1 (MQ=255)
                           tttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtg                                                                      >  1:962286/1‑100 (MQ=255)
                            ttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgc                                                                     <  2:618450/100‑1 (MQ=255)
                            ttGTTACATCAGCAAGTACCGAAAACACGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGCAGCGATGAAATCGGGAGCGCGGtgc                                                                     <  1:2278457/100‑1 (MQ=255)
                             tGTTACATCAGCAAGTACCGAAAACCGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtgca                                                                    <  2:672264/100‑1 (MQ=255)
                              gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                                   <  1:626639/100‑1 (MQ=255)
                              gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                                   >  1:473889/1‑100 (MQ=255)
                              gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                                   >  2:2175042/1‑100 (MQ=255)
                              gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                                   >  2:1055946/1‑100 (MQ=255)
                              gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGAGCGGtgcat                                                                   >  1:2616687/1‑100 (MQ=255)
                               ttACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatg                                                                  >  1:1748687/1‑100 (MQ=255)
                                tACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgc                                                                 <  1:2503126/100‑1 (MQ=255)
                                tACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgc                                                                 >  2:1256007/1‑100 (MQ=255)
                                tACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgc                                                                 <  2:1072504/100‑1 (MQ=255)
                                  cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                               <  1:2176689/100‑1 (MQ=255)
                                  cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                               >  1:1105904/1‑100 (MQ=255)
                                  cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                               >  1:2142203/1‑100 (MQ=255)
                                  cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                               >  1:2938518/1‑100 (MQ=255)
                                   aTCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGc                                                              >  2:2373240/1‑100 (MQ=255)
                                    tCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCt                                                             <  1:33216/100‑1 (MQ=255)
                                       gCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCg                                                          >  1:2067642/1‑100 (MQ=255)
                                       gCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCg                                                          <  2:763675/100‑1 (MQ=255)
                                       gCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCg                                                          >  2:1871623/1‑100 (MQ=255)
                                          aGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCtt                                                       >  1:363189/1‑100 (MQ=255)
                                          aGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCtt                                                       <  2:1908897/100‑1 (MQ=255)
                                           gTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTg                                                      <  1:1610563/100‑1 (MQ=255)
                                             aCCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                    >  1:518457/1‑100 (MQ=255)
                                             aCCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                    <  2:2872939/100‑1 (MQ=255)
                                             aCCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                    <  2:1020465/100‑1 (MQ=255)
                                              ccGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGc                                                   >  2:958890/1‑100 (MQ=255)
                                              ccGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGc                                                   <  2:1451108/100‑1 (MQ=255)
                                               cGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTGGCGCTGAAATGGGGAGCGCGGTGCATGCAGCATCGCTTGAGCg                                                  >  2:2490531/1‑100 (MQ=255)
                                               cGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCg                                                  >  2:2490734/1‑100 (MQ=255)
                                               cGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATACAGCTTCGCTTGAGCg                                                  <  1:1654693/100‑1 (MQ=255)
                                                gAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGt                                                 >  1:2707130/1‑100 (MQ=255)
                                                gAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGt                                                 >  1:1751179/1‑100 (MQ=255)
                                                  aaaCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGTCCTACTGTCGTAGCGATGAAAGGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTcc                                               <  1:3213256/100‑1 (MQ=255)
                                                  aaaCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTcc                                               <  1:2149043/100‑1 (MQ=255)
                                                  aaaCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTcc                                               <  1:2674198/100‑1 (MQ=255)
                                                     cAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGAGCGGTGCATGCAGCTTCGCTTGAGCGTCGAg                                             >  1:556700/1‑99 (MQ=255)
                                                       ggCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGa                                          >  2:328236/1‑100 (MQ=255)
                                                       ggCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGa                                          >  1:2435963/1‑100 (MQ=255)
                                                        gCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGaa                                         >  2:1694165/1‑100 (MQ=255)
                                                          gTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAAt                                       >  2:3048860/1‑100 (MQ=255)
                                                          gTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAAt                                       <  2:286931/100‑1 (MQ=255)
                                                            gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTa                                     >  1:1563642/1‑100 (MQ=255)
                                                            gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTa                                     <  2:758806/100‑1 (MQ=255)
                                                             cAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTaa                                    <  2:2153941/100‑1 (MQ=255)
                                                              aGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAAt                                   <  1:1426816/100‑1 (MQ=255)
                                                               gCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATg                                  >  2:1946673/1‑100 (MQ=255)
                                                               gCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATg                                  <  1:2736335/100‑1 (MQ=255)
                                                                cTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGa                                 >  2:537141/1‑100 (MQ=255)
                                                                  ggTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATa                               <  2:1980221/100‑1 (MQ=255)
                                                                    tCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGc                             <  1:603153/100‑1 (MQ=255)
                                                                     cGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCa                            >  2:3125296/1‑100 (MQ=255)
                                                                      gCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCAc                           >  2:1897514/1‑100 (MQ=255)
                                                                       ccTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGCGGAGCGCGGTGCATGCAGCTTCGCTTGACCGTCCAGTGAAATTAATGATAGCACt                          >  1:1234269/1‑100 (MQ=255)
                                                                       ccTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACt                          >  2:299315/1‑100 (MQ=255)
                                                                        cTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTg                         >  2:2085531/1‑100 (MQ=255)
                                                                        cTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTg                         <  2:1795797/100‑1 (MQ=255)
                                                                         tATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTgg                        <  2:491518/100‑1 (MQ=255)
                                                                         tATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTgg                        >  1:1188988/1‑100 (MQ=255)
                                                                          aTGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGga                       >  1:2917254/1‑100 (MQ=255)
                                                                             cGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgat                    >  2:2448975/1‑100 (MQ=255)
                                                                              gTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatg                   >  2:3203960/1‑100 (MQ=255)
                                                                                cGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatgat                 <  1:1571629/100‑1 (MQ=255)
                                                                                 ggAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGAtt                >  1:324024/1‑100 (MQ=255)
                                                                                  gaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTg               <  2:3262824/100‑1 (MQ=255)
                                                                                  gaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTg               >  2:1692725/1‑100 (MQ=255)
                                                                                  gaagaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTg               <  2:458795/100‑1 (MQ=255)
                                                                                  gaagaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTg               >  2:1660972/1‑100 (MQ=255)
                                                                                  gaagaaCCGCCCTACTTTCGTAGCGAAGAAATGGGGAGCACGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAGATTAATGATAGCACTGGAGATCAtt                >  1:226947/1‑99 (MQ=255)
                                                                                    agaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTACATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGc             <  1:2373223/100‑1 (MQ=255)
                                                                                       aCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAt          >  2:864666/1‑100 (MQ=255)
                                                                                       aCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAt          <  1:2509379/100‑1 (MQ=255)
                                                                                       aCCGCCCGACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAt          <  2:179481/100‑1 (MQ=255)
                                                                                        ccGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAtt         >  2:1150484/1‑100 (MQ=255)
                                                                                         cGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTa        >  2:113373/1‑100 (MQ=255)
                                                                                         cGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTa        >  2:177485/1‑100 (MQ=255)
                                                                                          gCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAc       <  2:3273293/100‑1 (MQ=255)
                                                                                          gCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAc       <  2:2435981/100‑1 (MQ=255)
                                                                                           cccTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACg      >  2:1493631/1‑100 (MQ=255)
                                                                                               |                                                                                                   
TGTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTC  >  NC_000913/2386270‑2386464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A