Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,917 G→A 60.8% A648V (GCG→GTG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,9170GA60.8% 80.2 / 156.5 141A648V (GCG→GTG) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (26/29);  new base A (49/36);  total (76/65)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

Only 100 of 144 total aligned reads displayed.

ATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTAAAC  >  NC_000913/2593818‑2594016
                                                                                                   |                                                                                                   
aTGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAgcg                                                                                                     <  1:1215496/100‑1 (MQ=255)
  gACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCAcc                                                                                                   <  2:1833782/100‑1 (MQ=255)
  gACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCAcc                                                                                                   <  2:490844/100‑1 (MQ=255)
   aCCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGcc                                                                                                   <  1:1516294/99‑1 (MQ=255)
    ccATGAGATAAAATTGAAGGAATCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGcc                                                                                                   <  1:1672801/98‑1 (MQ=255)
        gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGcgcg                                                                                             >  2:2104166/1‑100 (MQ=255)
           aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGcc                                                                                          <  2:1103823/100‑1 (MQ=255)
           aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                          >  2:1967241/1‑100 (MQ=255)
            tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCct                                                                                         >  2:2626655/1‑100 (MQ=255)
            tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCct                                                                                         >  2:3083601/1‑100 (MQ=255)
            tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCct                                                                                         >  1:1454658/1‑100 (MQ=255)
              aaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctct                                                                                       >  2:2419868/1‑100 (MQ=255)
                aTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTc                                                                                     >  2:3118195/1‑100 (MQ=255)
                   gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCg                                                                                  <  2:2958587/100‑1 (MQ=255)
                   gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                                  >  1:2324495/1‑100 (MQ=255)
                   gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                                  >  2:2755472/1‑100 (MQ=255)
                   gAAGGAGCCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAAACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                                  >  1:2552336/1‑100 (MQ=255)
                    aaGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGc                                                                                 >  2:2335353/1‑100 (MQ=255)
                     aGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCt                                                                                <  2:1274680/100‑1 (MQ=255)
                      ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTg                                                                               >  1:2204401/1‑100 (MQ=255)
                       gAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGa                                                                              >  2:670968/1‑100 (MQ=255)
                        aGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGAc                                                                             <  2:962002/100‑1 (MQ=255)
                         gTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACg                                                                            <  2:1504389/100‑1 (MQ=255)
                           cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACgg                                                                           >  1:2862079/1‑99 (MQ=255)
                           cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                          <  1:1022924/100‑1 (MQ=255)
                           cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                          >  1:1311328/1‑100 (MQ=255)
                           cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGa                                                                          >  1:626239/1‑100 (MQ=255)
                             gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                        >  2:2949042/1‑100 (MQ=255)
                              tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCa                                                                       >  1:511077/1‑100 (MQ=255)
                               gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGCAc                                                                         >  1:1150092/1‑97 (MQ=255)
                               gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                      >  1:2480150/1‑100 (MQ=255)
                               gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                      >  1:2863212/1‑100 (MQ=255)
                                aaaTAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGt                                                                     >  1:2922374/1‑100 (MQ=255)
                                   taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc                                                                  <  2:191548/100‑1 (MQ=255)
                                   taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc                                                                  <  1:1722105/100‑1 (MQ=255)
                                      ttGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATc                                                               <  2:2260633/100‑1 (MQ=255)
                                       tGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCt                                                              <  2:195993/100‑1 (MQ=255)
                                        gCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCtt                                                             >  1:1679665/1‑100 (MQ=255)
                                              gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                       <  1:3118168/100‑1 (MQ=255)
                                              gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                       >  1:1841279/1‑100 (MQ=255)
                                               cGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCt                                                      <  1:596334/100‑1 (MQ=255)
                                               cGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCt                                                      <  2:2447950/100‑1 (MQ=255)
                                               cGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCt                                                      <  2:511079/100‑1 (MQ=255)
                                                gTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTg                                                     <  2:2891397/100‑1 (MQ=255)
                                                 ttATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGa                                                    >  2:662729/1‑100 (MQ=255)
                                                   aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                                  <  1:2552970/100‑1 (MQ=255)
                                                   aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                                  >  1:430692/1‑100 (MQ=255)
                                                      cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                               >  1:1057636/1‑100 (MQ=255)
                                                      cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                               >  2:3385242/1‑100 (MQ=255)
                                                      cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                               >  1:3348790/1‑100 (MQ=255)
                                                       gATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGttt                                              >  2:2334305/1‑100 (MQ=255)
                                                        aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAACGtt                                               >  1:1232823/1‑98 (MQ=255)
                                                          cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg                                           >  1:3091868/1‑100 (MQ=255)
                                                          cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg                                           <  2:1617499/100‑1 (MQ=255)
                                                          cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCGTCTTCACGCTGACGGACCAGTAACATCTTGCGGCGTGAAAGTTTAAg                                           <  1:3105844/100‑1 (MQ=255)
                                                           gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgt                                          >  1:794647/1‑100 (MQ=255)
                                                              cAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGtgtg                                       <  1:1112491/100‑1 (MQ=255)
                                                              cAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGtgtg                                       <  2:2641753/100‑1 (MQ=255)
                                                               aGACGCGTAGTGCGCACACCATTAAGTGCGAACAGCACCTGCGCTGCCTCTTCCCGCTGACGGAGCAATATCATCTTGCGGCCTGAAAGTTTACGtgtg                                       <  1:2103049/99‑1 (MQ=255)
                                                                gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                     <  2:1057639/100‑1 (MQ=255)
                                                                gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                     >  2:1249668/1‑100 (MQ=255)
                                                                gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                     <  1:937930/100‑1 (MQ=255)
                                                                 aCGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAGCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTa                                    <  1:3122423/100‑1 (MQ=255)
                                                                  cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc                                   >  1:1241509/1‑100 (MQ=255)
                                                                   gCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACa                                  <  2:373567/100‑1 (MQ=255)
                                                                    cTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACAt                                 <  1:2209020/100‑1 (MQ=255)
                                                                     tCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATa                                <  1:2835361/100‑1 (MQ=255)
                                                                     tCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATa                                <  2:1989735/100‑1 (MQ=255)
                                                                     tCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGCAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATa                                <  1:1804715/100‑1 (MQ=255)
                                                                        gTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACt                             >  2:1708927/1‑100 (MQ=255)
                                                                        gTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGCCCAGTCACATCTTGCGGCCTGAAAGTTTAAGTGTGGTCCATAACt                             >  1:1132936/1‑100 (MQ=255)
                                                                        gTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATGTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACt                             >  1:713465/1‑100 (MQ=255)
                                                                          gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc                           >  2:2817361/1‑100 (MQ=255)
                                                                          gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCCGAAAGTTTAAGTGTGGTACATAACTgc                           >  2:2047199/1‑100 (MQ=255)
                                                                           cGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                          <  2:1607074/100‑1 (MQ=255)
                                                                           cGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                          <  2:328167/100‑1 (MQ=255)
                                                                            gAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgc                         <  1:2910525/100‑1 (MQ=255)
                                                                             aaCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTATCATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCa                        <  2:1505478/100‑1 (MQ=255)
                                                                             aaCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCa                        <  1:1679569/100‑1 (MQ=255)
                                                                              aCATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt                       <  1:3385213/100‑1 (MQ=255)
                                                                              aCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt                       >  1:1274843/1‑100 (MQ=255)
                                                                                 tcatTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCCCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGGGGTACATAACTGCGCATCAc                    <  1:1165148/100‑1 (MQ=255)
                                                                                 tcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCAc                    >  2:560528/1‑100 (MQ=255)
                                                                                   atTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTg                  <  1:1541433/100‑1 (MQ=255)
                                                                                    ttAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg                 <  2:514511/100‑1 (MQ=255)
                                                                                    ttAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg                 <  1:1641291/100‑1 (MQ=255)
                                                                                       aGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACGTCATGCGGCCGGAAAGGGTAAGTGTGGTACATAGCTGAGCATCACTgg                 >  1:3333571/1‑97 (MQ=255)
                                                                                        gTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtt             >  1:1139638/1‑100 (MQ=255)
                                                                                        gTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtt             >  1:1748711/1‑100 (MQ=255)
                                                                                        gTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtt             >  2:2135811/1‑100 (MQ=255)
                                                                                          gCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt           >  2:248471/1‑100 (MQ=255)
                                                                                           cGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGttttt          >  2:1303804/1‑100 (MQ=255)
                                                                                            gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGCCCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCAGTGGTGTTTTTc         <  1:1781376/100‑1 (MQ=255)
                                                                                             aaCAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCt        >  2:3195121/1‑100 (MQ=255)
                                                                                             aaCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCt        <  1:800711/100‑1 (MQ=255)
                                                                                              aCAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg       <  1:2206437/100‑1 (MQ=255)
                                                                                              aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg       <  2:1663043/100‑1 (MQ=255)
                                                                                              aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg       >  2:92202/1‑100 (MQ=255)
                                                                                                aGCGCCTGCGCGGCCTCATCCCGCTGACGGACCAGTAACAGCGTGGGGTCTGAGAGTTTGAGTGTGGTACATAACTGTGCATCACTGGCGTTTTTCTg       <  1:65492/98‑1 (MQ=255)
                                                                                                   gcCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTAAAc  >  2:59493/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTAAAC  >  NC_000913/2593818‑2594016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A