Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,917 G→A 100% A648V (GCG→GTG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,9170GA99.1% 348.2 / ‑4.8 108A648V (GCG→GTG) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (43/63);  total (45/63)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 108 total aligned reads displayed.

GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTA  >  NC_000913/2593820‑2594013
                                                                                                 |                                                                                                
gACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCAcc                                                                                                >  2:1282833/1‑100 (MQ=255)
    aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGCACAGCACCTgcg                                                                                            <  2:378247/100‑1 (MQ=255)
    aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGAGCGCGCAGACGCTCAGTGAGAACATCATTAAGTGCGAACAGCACCTgcg                                                                                            >  2:783041/1‑100 (MQ=255)
      gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGcgcg                                                                                          >  1:162103/1‑100 (MQ=255)
      gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTAAGTACGAACATAATTAAGTGCGAACAGCACCTGcgcg                                                                                          >  1:412411/1‑100 (MQ=255)
       agaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCgg                                                                                         <  1:2268432/100‑1 (MQ=255)
        gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCgg                                                                                         <  2:2400735/99‑1 (MQ=255)
        gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGc                                                                                        <  1:1842720/100‑1 (MQ=255)
         aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                       >  1:553114/1‑100 (MQ=255)
         aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                       <  2:673775/100‑1 (MQ=255)
          tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCct                                                                                      <  2:2377636/100‑1 (MQ=255)
           aaaaTTGAAGGAGTCCGTGAAATAATTGCCATTACGTTATGCGATAGCGCAGACGCTAAGTGCGAAACTCATTACGTGCGAACAGCACCTGCGCGGCctc                                                                                     >  2:523485/1‑100 (MQ=255)
           aaaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctc                                                                                     <  1:76929/100‑1 (MQ=255)
             aaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATAGCGCAGACGCTCAGTGCGAACATCATTATGTGCGAACAGCACCTGCGCGGCCTCtt                                                                                   >  2:1445630/1‑100 (MQ=255)
                 gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                               >  2:1807385/1‑100 (MQ=255)
                 gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                               >  2:1112339/1‑100 (MQ=255)
                   aGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCt                                                                             <  2:433804/100‑1 (MQ=255)
                   aGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCt                                                                             <  2:1168060/100‑1 (MQ=255)
                   aGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCt                                                                             <  2:557328/100‑1 (MQ=255)
                    ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTg                                                                            <  2:1127391/100‑1 (MQ=255)
                    ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTg                                                                            >  1:1127399/1‑100 (MQ=255)
                     gAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGa                                                                           <  1:1672958/100‑1 (MQ=255)
                     gAGGCAGGGAAATAATTGCCATTGCGTGATGCGATCGCGCAGACGCTCAGTGCGAACATCATTATGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCt                                                                             <  2:1539147/98‑1 (MQ=255)
                       gTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACg                                                                         <  2:558672/100‑1 (MQ=255)
                       gTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACg                                                                         >  2:1716221/1‑100 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGAGGCCTCTTCCCGCTGACGGa                                                                       >  1:2354277/1‑100 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGa                                                                       >  2:1008939/1‑100 (MQ=255)
                          aGTGAAATAATTGCCATTGCGTTATGCGATCGCG‑AGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                     <  2:1821437/100‑1 (MQ=255)
                           gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                     >  2:1836098/1‑100 (MQ=255)
                           gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                     <  1:159434/100‑1 (MQ=255)
                           gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAc                                                                      >  2:551814/1‑99 (MQ=255)
                            tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAc                                                                      >  2:2548827/1‑98 (MQ=255)
                            tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCa                                                                    >  2:2068024/1‑100 (MQ=255)
                            tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCa                                                                    >  1:656109/1‑100 (MQ=255)
                              aaaTAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCact                                                                  <  1:1741572/100‑3 (MQ=255)
                               aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                 <  1:1055970/100‑1 (MQ=255)
                                 taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc                                                               <  1:789276/100‑1 (MQ=255)
                                 taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc                                                               <  1:1496983/100‑1 (MQ=255)
                                  aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCATTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa                                                              <  1:2439064/100‑1 (MQ=255)
                                  aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTATGTGCGAACAGCCCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                 >  2:1224791/1‑97 (MQ=255)
                                       ccATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTg                                                         <  1:1777241/100‑1 (MQ=255)
                                        cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTTACATCTTGc                                                        >  2:724460/1‑100 (MQ=255)
                                        cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc                                                        >  2:625597/1‑100 (MQ=255)
                                          ttGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCgg                                                      <  1:40819/100‑1 (MQ=255)
                                          ttGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCgg                                                      <  1:1482949/100‑1 (MQ=255)
                                          ttGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCgg                                                      <  1:683400/100‑1 (MQ=255)
                                            gCGTTATGTGATCTCTCAGACGCTCATTGCGAACACCATTAATTGCGTACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    <  2:2166604/100‑1 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    >  1:748737/1‑100 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    >  1:87670/1‑100 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    <  2:193907/100‑1 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    <  1:1618078/100‑1 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGcc                                                    <  2:1409337/100‑1 (MQ=255)
                                             cGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCt                                                   <  2:2076517/100‑1 (MQ=255)
                                              gTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTg                                                  <  1:1157784/100‑1 (MQ=255)
                                                  tGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                              <  1:1355881/100‑1 (MQ=255)
                                                  tGCGATAGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                              <  2:617597/100‑1 (MQ=255)
                                                    cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                            >  2:1778437/1‑100 (MQ=255)
                                                    cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                            <  2:454166/100‑1 (MQ=255)
                                                      aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa                                          >  1:1002928/1‑100 (MQ=255)
                                                      aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa                                          <  1:2107382/100‑1 (MQ=255)
                                                        cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg                                        <  2:2473184/100‑1 (MQ=255)
                                                        cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg                                        <  1:1162001/100‑1 (MQ=255)
                                                         gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgt                                       >  1:1182379/1‑100 (MQ=255)
                                                          cgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgtg                                      <  1:1778450/100‑1 (MQ=255)
                                                             aGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTgg                                   <  1:1069956/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                  <  2:2457262/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                  <  2:1712823/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                  >  2:488371/1‑100 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                  >  1:2403132/1‑100 (MQ=255)
                                                                cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAGATCTTGCGGCCTGAAAGCTTAAGTGTGGtcc                                <  1:1731395/100‑3 (MQ=255)
                                                                cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc                                >  2:2150320/1‑100 (MQ=255)
                                                                   tCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATa                             <  2:302558/100‑1 (MQ=255)
                                                                   tCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATa                             <  2:1701935/100‑1 (MQ=255)
                                                                      gTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTATCATCGTGCGGCCTGAAAGTTTAAGTGTGGTACATAACt                          >  2:507714/1‑100 (MQ=255)
                                                                       tGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTg                         >  1:854586/1‑100 (MQ=255)
                                                                        gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc                        >  1:1502546/1‑100 (MQ=255)
                                                                        gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc                        >  1:758700/1‑100 (MQ=255)
                                                                         cGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                       <  1:435689/100‑1 (MQ=255)
                                                                         cGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                       <  1:503548/100‑1 (MQ=255)
                                                                          gAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgc                      >  2:333437/1‑100 (MQ=255)
                                                                          gAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgc                      >  2:195824/1‑100 (MQ=255)
                                                                          gAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgc                      <  2:1666351/100‑1 (MQ=255)
                                                                            aCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt                    <  1:2001043/100‑1 (MQ=255)
                                                                            aCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt                    <  1:193924/100‑1 (MQ=255)
                                                                               tcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCa                  >  2:1244098/1‑99 (MQ=255)
                                                                               tcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCa                  <  1:736752/99‑1 (MQ=255)
                                                                                 atTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTg               <  2:162101/100‑1 (MQ=255)
                                                                                  ttAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg              <  2:40284/100‑1 (MQ=255)
                                                                                   tAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGc             >  2:1561936/1‑100 (MQ=255)
                                                                                    aaGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCg            <  1:1618168/100‑1 (MQ=255)
                                                                                     aGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCCTCACTGGCg            >  2:593540/1‑99 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt        <  1:2068038/100‑1 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt        >  1:4794/1‑100 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt        >  1:1818137/1‑100 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt        >  1:1484501/1‑100 (MQ=255)
                                                                                          gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc      <  1:749633/100‑1 (MQ=255)
                                                                                           aaCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCt     <  2:656102/100‑1 (MQ=255)
                                                                                             cAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGt   >  1:1099692/1‑100 (MQ=255)
                                                                                             cAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGt   <  1:950817/100‑1 (MQ=255)
                                                                                              aGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTa  >  1:67383/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTA  >  NC_000913/2593820‑2594013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A