Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,917 G→A 23.1% A648V (GCG→GTG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,9170GA23.1% 342.5 / 93.8 177A648V (GCG→GTG) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (74/62);  new base A (23/18);  total (97/80)
Fisher's exact test for biased strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 179 total aligned reads displayed.

GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTAAA  >  NC_000913/2593820‑2594015
                                                                                                 |                                                                                                  
gACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGcc                                                                                                  <  2:409809/100‑1 (MQ=255)
gACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGcc                                                                                                  <  1:1283713/100‑1 (MQ=255)
 aCCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCt                                                                                                 <  1:3054830/100‑1 (MQ=255)
  ccATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTg                                                                                                >  1:480927/1‑100 (MQ=255)
   cATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTgc                                                                                               >  1:1108042/1‑100 (MQ=255)
   cATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTgc                                                                                               <  2:3006731/100‑1 (MQ=255)
   cATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGAGTTATGCGATCGCGCAGACGCTCAGTGCGAACAGCATTAAGTGCGAACAGCGCCTgc                                                                                               >  2:1980387/1‑100 (MQ=255)
    aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTgcg                                                                                              <  2:1462133/100‑1 (MQ=255)
    aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgcg                                                                                              <  1:1618028/100‑1 (MQ=255)
      gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGcgcg                                                                                            <  2:1549135/100‑1 (MQ=255)
       agaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCgg                                                                                           <  1:2639202/100‑1 (MQ=255)
        gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGc                                                                                          >  1:4132078/1‑100 (MQ=255)
         aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGcc                                                                                         >  2:3137130/1‑100 (MQ=255)
          tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                         >  2:3547041/1‑99 (MQ=255)
            aaaTTGAAGGAGTCAGTGAAATAATTGCCAGTGGGATATGCGATCGCGCAAACGCTGAGTGCCAACATCAGTAAGTGCGAACAGCGCATGCGCGGCct                                                                                        >  2:2485224/1‑98 (MQ=255)
             aaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCtt                                                                                     >  2:1657900/1‑100 (MQ=255)
              aTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTc                                                                                    <  1:2133395/100‑1 (MQ=255)
                    ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTg                                                                              <  2:1828828/100‑1 (MQ=255)
                    ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTg                                                                              >  1:687895/1‑100 (MQ=255)
                     gAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGa                                                                             <  2:789329/100‑1 (MQ=255)
                      aGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGAc                                                                            <  2:278689/100‑1 (MQ=255)
                      aGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGAc                                                                            <  2:2204572/100‑1 (MQ=255)
                        tCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACgg                                                                          <  2:603663/100‑1 (MQ=255)
                        tCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACgg                                                                          <  2:1861703/100‑1 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                         <  2:2915518/100‑1 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                         >  2:681407/1‑100 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                         >  1:1537388/1‑100 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGa                                                                         >  2:1488848/1‑100 (MQ=255)
                         cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGa                                                                         >  2:3018261/1‑100 (MQ=255)
                           gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                       <  1:607461/100‑1 (MQ=255)
                           gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTAACGGAcc                                                                       >  2:2214545/1‑100 (MQ=255)
                            tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCa                                                                      >  1:37674/1‑100 (MQ=255)
                             gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                     >  2:1396986/1‑100 (MQ=255)
                             gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                     <  1:92716/100‑1 (MQ=255)
                              aaaTAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGt                                                                    >  1:2941988/1‑100 (MQ=255)
                              aaaTAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGt                                                                    >  2:3974623/1‑100 (MQ=255)
                               aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                   >  1:3340877/1‑100 (MQ=255)
                               aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                   >  1:3852927/1‑100 (MQ=255)
                                ataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTaa                                                                  >  2:1736577/1‑100 (MQ=255)
                                 taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTaa                                                                  >  2:3405762/1‑99 (MQ=255)
                                  aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa                                                                <  2:2711612/100‑1 (MQ=255)
                                  aatTGCCATTGCGTTATGCGAACGCGCAGAAGCTCAGAGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGACTCTTCCCGCTGACGGACCAGTAACa                                                                >  2:3190248/1‑100 (MQ=255)
                                        cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc                                                          >  1:1390994/1‑100 (MQ=255)
                                        cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc                                                          <  1:267328/100‑1 (MQ=255)
                                           tGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGc                                                       >  2:3524142/1‑100 (MQ=255)
                                            gCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCGTGCGGcc                                                      <  1:2167743/100‑1 (MQ=255)
                                                tATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaa                                                  >  1:3726957/1‑100 (MQ=255)
                                                 aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                                 >  1:980127/1‑100 (MQ=255)
                                                  tGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                                <  1:4053135/100‑1 (MQ=255)
                                                  tGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                                <  1:1154629/100‑1 (MQ=255)
                                                  tGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGAGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                                >  2:2293839/1‑100 (MQ=255)
                                                  tGCGATCGCGCAGACGCTCAGGGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                                <  2:3229837/100‑1 (MQ=255)
                                                   gCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTCACGGACCAGTAACATCTTGCGGCCTGAAAg                                                >  2:1007357/1‑99 (MQ=255)
                                                   gCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGt                                               >  1:1581526/1‑100 (MQ=255)
                                                    cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                              >  1:1006841/1‑100 (MQ=255)
                                                    cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                              <  1:1306446/100‑1 (MQ=255)
                                                    cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                              >  2:636656/1‑100 (MQ=255)
                                                     gATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGttt                                             >  1:3304490/1‑100 (MQ=255)
                                                      aTCGCGCAGACGCTCAGTGCGAACATCATTAATTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAAtt                                              >  2:3942889/1‑98 (MQ=255)
                                                      aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa                                            >  2:413975/1‑100 (MQ=255)
                                                      aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa                                            >  2:414689/1‑100 (MQ=255)
                                                         gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCTGCCTGAAAGTTTAAg                                          <  2:3802757/99‑1 (MQ=255)
                                                         gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgt                                         >  2:2076463/1‑100 (MQ=255)
                                                         gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgt                                         >  1:2993723/1‑100 (MQ=255)
                                                         gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg                                          >  1:3677742/1‑99 (MQ=255)
                                                          cgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgtg                                        <  2:37674/100‑1 (MQ=255)
                                                          cgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgtg                                        <  2:2523481/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTgg                                     <  2:1071853/99‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                    <  2:973775/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                    <  2:440076/100‑1 (MQ=255)
                                                              gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt                                    <  1:3974649/100‑1 (MQ=255)
                                                                cGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTa                                   <  2:3677718/99‑1 (MQ=255)
                                                                cGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc                                  <  1:1527592/100‑1 (MQ=255)
                                                                  cTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACAt                                >  2:3582690/1‑100 (MQ=255)
                                                                       tGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTg                           <  2:2121624/100‑1 (MQ=255)
                                                                        gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc                          >  2:1278538/1‑100 (MQ=255)
                                                                         cGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                         >  2:1653005/1‑100 (MQ=255)
                                                                         cGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                         <  2:4089895/100‑1 (MQ=255)
                                                                          gAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgc                        <  1:1932749/100‑1 (MQ=255)
                                                                             catcatTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATc                     >  1:228304/1‑100 (MQ=255)
                                                                              atcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCa                    >  1:74794/1‑100 (MQ=255)
                                                                                catTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACt                  >  1:1023447/1‑100 (MQ=255)
                                                                                catTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACt                  >  1:2713512/1‑100 (MQ=255)
                                                                                 atTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCAc                   <  2:705634/98‑1 (MQ=255)
                                                                                 atTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTg                 <  1:364209/100‑1 (MQ=255)
                                                                                 atTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTg                 <  1:1056741/100‑1 (MQ=255)
                                                                                  ttAAGTGAGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg                >  1:2174585/1‑100 (MQ=255)
                                                                                   tAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGc               >  2:2750134/1‑100 (MQ=255)
                                                                                    aaGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCg              <  1:3560843/100‑1 (MQ=255)
                                                                                      gTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGCGGTACATAACTGCGCATCACTGGCGtt            >  2:397398/1‑100 (MQ=255)
                                                                                       tGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGttt           >  2:883553/1‑100 (MQ=255)
                                                                                       tGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTGTAAGTGTGGTACATAACTGCGCATCACTGGCGttt           >  2:460082/1‑100 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt          <  1:2431085/100‑1 (MQ=255)
                                                                                        gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt          >  1:3930984/1‑100 (MQ=255)
                                                                                         cGAACAGCGCCTGCGCGGCCTCTTCCCGCCGACGGCCCCGTAACGTCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGttttt         <  2:3264262/100‑1 (MQ=255)
                                                                                          gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc        <  2:3640102/100‑1 (MQ=255)
                                                                                          gAACAACGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAAATCTTGCGGCCCGAAAGTATAAGTGTGGTAAATAACTGCGCATAACTGGCGttttt         >  1:628099/1‑99 (MQ=255)
                                                                                            aCAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg      <  1:2458609/100‑1 (MQ=255)
                                                                                            aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg      <  2:1579681/100‑1 (MQ=255)
                                                                                                cgcCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTaaa  >  2:2146586/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTAAA  >  NC_000913/2593820‑2594015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A