Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,028 G→A 67.4% R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,0280GA67.4% 145.6 / 119.3 135R15C (CGT→TGT) sdhEflavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base G (20/24);  new base A (42/49);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 144 total aligned reads displayed.

CAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTT  >  NC_000913/3040930‑3041127
                                                                                                  |                                                                                                   
cagcagACGAATAAAGATGCGTTTTTTTTTTTTTTTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcg                                                                                                     >  1:688847/1‑99 (MQ=255)
 agcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGCCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagc                                                                                                   <  1:19521/100‑1 (MQ=255)
  gcagACGCATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTAATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCAGACCAcagcg                                                                                                  <  1:2319677/100‑1 (MQ=255)
   cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcggcgg                                                                                                 <  1:1313462/100‑1 (MQ=255)
   cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTACGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg                                                                                                 <  1:878758/100‑1 (MQ=255)
     gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCa                                                                                               <  2:828617/100‑1 (MQ=255)
     gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCa                                                                                               <  1:576615/100‑1 (MQ=255)
     gACGAATAAAGATGCGTTTTTCGTCATCGCTGAAGCTGGCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCa                                                                                               <  1:96936/100‑1 (MQ=255)
      aCGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCAt                                                                                              <  2:1190112/100‑1 (MQ=255)
       cGAATAAAGATGCGTTTTTCGTAATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATg                                                                                             >  1:1716927/1‑100 (MQ=255)
         aaTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGcc                                                                                           >  1:2058947/1‑100 (MQ=255)
          aTAAAGCTGCGTTTTTCGTCATCGCCTAAGCTATCGTACTCATGTTCGCAAAACGGCATGATTGACACCTCGAGTTCGCGCATACAACAGCGGCATGccc                                                                                          <  1:1512379/100‑1 (MQ=255)
             aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaat                                                                                       >  1:664498/1‑100 (MQ=255)
             aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat                                                                                       >  1:1153852/1‑100 (MQ=255)
             aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat                                                                                       <  1:3287930/100‑1 (MQ=255)
             aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat                                                                                       <  2:432714/100‑1 (MQ=255)
              aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatg                                                                                      <  2:171573/100‑1 (MQ=255)
               gATGCGTTTTTCTTTATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                      >  1:1182995/1‑99 (MQ=255)
               gATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                      >  2:2530356/1‑99 (MQ=255)
                  gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaatg                                                                                  >  2:3162883/1‑100 (MQ=255)
                     tttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCgg                                                                               <  1:433076/100‑1 (MQ=255)
                     tttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCgg                                                                               <  1:1738898/100‑1 (MQ=255)
                      ttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCggg                                                                              <  2:70925/100‑1 (MQ=255)
                       tttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGc                                                                             <  1:2545899/100‑1 (MQ=255)
                          cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCttt                                                                          <  2:881291/100‑1 (MQ=255)
                          cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCttt                                                                          <  2:3084968/100‑1 (MQ=255)
                           gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttg                                                                         >  2:1572207/1‑100 (MQ=255)
                           gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttg                                                                         >  2:1497304/1‑100 (MQ=255)
                           gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGCTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttg                                                                         >  1:2385572/1‑100 (MQ=255)
                            tCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgt                                                                        <  2:2745820/100‑1 (MQ=255)
                            tCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgt                                                                        >  2:1529291/1‑100 (MQ=255)
                            tCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgt                                                                        >  1:2190816/1‑100 (MQ=255)
                              aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                      <  2:1022520/100‑1 (MQ=255)
                              aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                      <  2:1444131/100‑1 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                    <  1:2000094/100‑1 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                    <  2:1150368/100‑1 (MQ=255)
                                 gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                   >  1:3308596/1‑100 (MQ=255)
                                 gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                   >  1:1240486/1‑100 (MQ=255)
                                  cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                                  >  2:2135870/1‑100 (MQ=255)
                                       gCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTcc                                                             <  1:18741/100‑1 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                            <  1:2836807/100‑1 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAGTGCGGGCTTTGTTGTTAATGTCCa                                                            >  1:2113583/1‑100 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                            <  1:2461222/100‑1 (MQ=255)
                                          gTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtc                                                          <  2:572764/100‑1 (MQ=255)
                                          gTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtc                                                          <  2:232535/100‑1 (MQ=255)
                                           tCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct                                                         <  2:2140492/100‑1 (MQ=255)
                                             gTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttc                                                       <  1:2832681/100‑1 (MQ=255)
                                              tACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttct                                                      <  1:96642/100‑1 (MQ=255)
                                               aCTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATcttctt                                                     >  1:356606/1‑100 (MQ=255)
                                                cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                    >  2:662307/1‑100 (MQ=255)
                                                cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                    <  1:3113216/100‑1 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                  >  2:2587078/1‑100 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                  >  1:2621057/1‑100 (MQ=255)
                                                    tGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGt                                                >  1:2636158/1‑100 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCCTCCCACAGCGGCATGCTCAACGAATGAGGGCTTTGTTCATAATGTCCATcttcttattgt                                                >  2:991252/1‑94 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                               <  2:920075/100‑1 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                               >  2:3146439/1‑100 (MQ=255)
                                                     gTTCGAAAAACGGCATGAGTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATACGGGCTTTGTTGTTAATGTCCATTTTCTTCCTGtc                                               >  1:559749/1‑100 (MQ=255)
                                                      ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                              >  2:2419698/1‑100 (MQ=255)
                                                        cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                            >  1:2169127/1‑100 (MQ=255)
                                                         gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                           >  2:3007735/1‑100 (MQ=255)
                                                         gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                           <  1:55527/100‑1 (MQ=255)
                                                          aaaaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACg                                          >  1:892947/1‑100 (MQ=255)
                                                             aaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaa                                       <  1:2808408/100‑1 (MQ=255)
                                                              aCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaaa                                      >  2:1208735/1‑100 (MQ=255)
                                                                 gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                   <  2:1386868/100‑1 (MQ=255)
                                                                  cATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                   <  2:2762033/99‑1 (MQ=255)
                                                                  cATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                  <  1:1572200/100‑1 (MQ=255)
                                                                   aTGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                                 <  2:943501/100‑1 (MQ=255)
                                                                   aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                                 <  2:2746271/100‑1 (MQ=255)
                                                                    tGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                                 >  1:1266348/1‑99 (MQ=255)
                                                                     gATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                               <  2:569001/100‑1 (MQ=255)
                                                                     gATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                               >  2:1479024/1‑100 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                              >  2:3088252/1‑100 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                               >  2:2776981/1‑99 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                              >  1:2949483/1‑100 (MQ=255)
                                                                       ttGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAcc                             >  2:493328/1‑100 (MQ=255)
                                                                         gAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                           >  1:230693/1‑100 (MQ=255)
                                                                         gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGCAAATCCAGTACCCg                           >  1:2757927/1‑100 (MQ=255)
                                                                         gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                               >  1:1213070/1‑96 (MQ=255)
                                                                         gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                           >  2:1789713/1‑100 (MQ=255)
                                                                           aaTATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc                         <  2:1829508/100‑1 (MQ=255)
                                                                            atatCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCt                        >  1:197230/1‑100 (MQ=255)
                                                                              atCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAt                      <  2:1329292/100‑1 (MQ=255)
                                                                                 gAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGt                   >  2:1147885/1‑100 (MQ=255)
                                                                                  aGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa                  >  2:2201450/1‑100 (MQ=255)
                                                                                  aGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa                  <  1:2049044/100‑1 (MQ=255)
                                                                                     tCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg               <  2:2452755/100‑1 (MQ=255)
                                                                                       gcgcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtg             <  2:2385589/100‑1 (MQ=255)
                                                                                        cgcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtgt            >  2:2314363/1‑100 (MQ=255)
                                                                                         gcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGtt           <  1:1011279/100‑1 (MQ=255)
                                                                                          cATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttt          <  1:627711/100‑1 (MQ=255)
                                                                                              ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg      >  1:733575/1‑100 (MQ=255)
                                                                                              ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAGCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg      <  1:3329576/100‑1 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg      <  2:1856859/100‑1 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg      >  2:1759398/1‑100 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg      <  1:3087949/100‑1 (MQ=255)
                                                                                                aCGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGcc    <  1:493326/100‑1 (MQ=255)
                                                                                                 cggcggCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCct   >  2:21738/1‑100 (MQ=255)
                                                                                                  ggcggcATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCctt  <  1:1659008/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTT  >  NC_000913/3040930‑3041127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A