Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,028 G→A 100% R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,0280GA98.1% 301.6 / ‑3.9 97R15C (CGT→TGT) sdhEflavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (51/43);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGC  >  NC_000913/3040931‑3041124
                                                                                                 |                                                                                                
agcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCTCCCATACCAcagc                                                                                                >  2:1547859/1‑100 (MQ=255)
agcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagc                                                                                                <  1:788442/100‑1 (MQ=255)
 gcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcg                                                                                               <  2:2711534/100‑1 (MQ=255)
    gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCa                                                                                            <  1:1161658/100‑1 (MQ=255)
     aCGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACagcggc                                                                                             <  1:3044949/98‑1 (MQ=255)
      ctaaTAAAGATGCGTTTTTGGTCATGGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg                                                                                              <  2:2411277/94‑1 (MQ=255)
      cGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATg                                                                                          >  1:1215885/1‑100 (MQ=255)
       gAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGc                                                                                         <  1:2426148/100‑1 (MQ=255)
        aaTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGcn                                                                                        <  2:1028300/100‑2 (MQ=255)
         aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc                                                                                       <  1:642204/100‑1 (MQ=255)
         aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTAATCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc                                                                                       <  1:765043/100‑1 (MQ=255)
            aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat                                                                                    <  2:1948243/100‑1 (MQ=255)
              gATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatga                                                                                  >  1:112900/1‑100 (MQ=255)
                 gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaatg                                                                               >  2:2658646/1‑100 (MQ=255)
                   gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCg                                                                             <  2:916611/100‑1 (MQ=255)
                     ttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCggg                                                                           <  2:881932/100‑1 (MQ=255)
                         cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCttt                                                                       >  2:822641/1‑100 (MQ=255)
                          gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttg                                                                      >  2:758322/1‑100 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                    >  1:710757/1‑100 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                    >  1:1892673/1‑100 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                    >  1:664309/1‑100 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                    <  2:2231222/100‑1 (MQ=255)
                             aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGACATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                   <  1:1656923/100‑1 (MQ=255)
                              tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                   <  1:563285/99‑1 (MQ=255)
                              tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTtgttgt                                                                  >  2:548756/1‑100 (MQ=255)
                                ncTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                 >  2:192630/2‑99 (MQ=255)
                                 cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                               <  2:2051794/100‑1 (MQ=255)
                                 cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                               <  2:2051342/100‑1 (MQ=255)
                                 cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                               <  1:41688/100‑1 (MQ=255)
                                   tAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATg                                                             <  1:105664/100‑1 (MQ=255)
                                      gCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTcc                                                          <  2:1987548/100‑1 (MQ=255)
                                       cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                         >  2:434216/1‑100 (MQ=255)
                                         gTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtc                                                       >  2:14541/1‑100 (MQ=255)
                                         gTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtc                                                       >  2:2431643/1‑100 (MQ=255)
                                           cGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtctt                                                     >  2:2354851/1‑100 (MQ=255)
                                            gTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATATCCAtctt                                                     >  1:279224/1‑99 (MQ=255)
                                             tACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttct                                                   >  1:1464381/1‑100 (MQ=255)
                                                 cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                               >  1:2324044/1‑100 (MQ=255)
                                                  aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCTCGCATACCACACCTGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                              >  1:1102190/1‑100 (MQ=255)
                                                  aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                              <  1:2851292/100‑1 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTTGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                            <  1:360911/100‑1 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCTCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                            <  2:2818165/100‑1 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                            <  1:434219/100‑1 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                            >  1:2403206/1‑100 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                            <  1:1158890/100‑1 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTACATCTTCTTCCTGt                                             >  1:170474/1‑99 (MQ=255)
                                                     ttCGAAAAACGGCATGATTTAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTTTTAATGTCCATCTTCTTCCTGtc                                            <  2:2645695/99‑1 (MQ=255)
                                                     ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                           <  2:1623911/100‑1 (MQ=255)
                                                      tCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtctc                                          >  1:913481/1‑100 (MQ=255)
                                                       cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                         <  2:2803974/100‑1 (MQ=255)
                                                       cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCAGACTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                         >  1:2534642/1‑100 (MQ=255)
                                                        gAAAATCGGCATGATTGATATATCTTGTTCGCGCATACCATAGTGGCATGCCCAATGAATGCGGGCTTTCGTGTTAATGTCCATCTTCTTCCTGTCTCAc                                        <  1:2747383/100‑1 (MQ=255)
                                                        gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                        <  2:2403178/100‑1 (MQ=255)
                                                        gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                        >  2:946152/1‑100 (MQ=255)
                                                        gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTATTCCTGTCTCAc                                        >  1:2802263/1‑100 (MQ=255)
                                                           aaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGACATGCCCAATGAATGCGGGCTTTGTTAGTAATGTCCATCTTCTTCCTGTCTCACGaa                                     >  2:2938122/1‑100 (MQ=255)
                                                             aCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaaa                                   <  2:476284/100‑1 (MQ=255)
                                                              cGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAAt                                  <  2:2266561/100‑1 (MQ=255)
                                                               ggtaTGATTGAAATATCTATTTCGCGCTTATCACTGTTGCATGTCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                 <  1:174457/97‑1 (MQ=255)
                                                               ggCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                 <  1:2259012/100‑1 (MQ=255)
                                                               ggCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                 <  1:1677035/100‑1 (MQ=255)
                                                                gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                <  1:758326/100‑1 (MQ=255)
                                                                 cATGATTGAAATATCGAGTTCGCTCATACCCTAGCGGCATGCCCATTGAATGCGGGCTTTGTTGTTAATGTCTATCTTCTTCCTGTCTCACGAAAAt                                  <  2:52127/97‑1 (MQ=255)
                                                                  aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                              <  1:2838830/100‑1 (MQ=255)
                                                                   tGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGt                             >  1:349679/1‑100 (MQ=255)
                                                                   tGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCATGTCTCACGAAAATCCa                               >  1:2123932/1‑98 (MQ=255)
                                                                     aTTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                           >  2:1550192/1‑100 (MQ=255)
                                                                      ttGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAcc                          >  2:1396078/1‑100 (MQ=255)
                                                                      ttGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAcc                          >  1:2517629/1‑100 (MQ=255)
                                                                      ttGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAcc                          >  2:256211/1‑100 (MQ=255)
                                                                       tGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATATCCCTCTTCTTCCTGTCTCACGAAAATCCAGTAccc                         >  2:1475253/1‑100 (MQ=255)
                                                                        gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCATTGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAACCg                        >  1:999074/1‑100 (MQ=255)
                                                                        gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                        >  1:857257/1‑100 (MQ=255)
                                                                        gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                        >  1:1945481/1‑100 (MQ=255)
                                                                         aaaTATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCgg                       >  2:163088/1‑100 (MQ=255)
                                                                          aaTATCGAGTTCGCGCATACTACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc                      <  1:266991/100‑1 (MQ=255)
                                                                              tCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAtt                  >  1:788726/1‑100 (MQ=255)
                                                                               cGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTg                 <  2:1173533/100‑1 (MQ=255)
                                                                               cGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTg                 >  2:2940831/1‑100 (MQ=255)
                                                                               cGAGTTCGCGCATACCACAACGACATAGCCAATGAACGCAGGCTCAGTTGTTAATGTCCATCTTAGTCCTGTCTCACGAAAATCCAGTAACCGGCTATTg                 >  2:180737/1‑100 (MQ=255)
                                                                                 aGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGt                <  2:594244/99‑1 (MQ=255)
                                                                                 aGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa               <  1:2030902/100‑1 (MQ=255)
                                                                                  gTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTaa              >  2:1635170/1‑100 (MQ=255)
                                                                                  gTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTACTGTATCACGAAAATCCAGTACCCGGCTATTGTaa              >  1:2537277/1‑100 (MQ=255)
                                                                                   ttCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa               <  2:1024135/98‑1 (MQ=255)
                                                                                   ttCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAAc             <  2:1215874/100‑1 (MQ=255)
                                                                                    tCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg            >  2:113310/1‑100 (MQ=255)
                                                                                    tCGCGCATAACACAACGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAATTCCAGTACCCGGCTATTGTAACg            <  2:537492/100‑1 (MQ=255)
                                                                                     cgcgCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgt           >  2:1918027/1‑100 (MQ=255)
                                                                                     cgcgCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgt           >  1:2839577/1‑100 (MQ=255)
                                                                                     cgcgCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTATAAAGAAAATCCAGTACCCGGCTATTGTAACgt           >  1:1149563/1‑100 (MQ=255)
                                                                                       cgcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtgt         >  2:2333240/1‑100 (MQ=255)
                                                                                        gtaTACCACAGCGGCATGCCCACCTAATGCCGGCTTGGTTGATAATGTCCATCTTCTCCCTTTCTCACGAAATTCCAGTACCCGGCTATTGTAACgtt          <  2:269456/96‑2 (MQ=255)
                                                                                        gcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGtt        <  2:529348/100‑1 (MQ=255)
                                                                                         cATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttt       >  1:2552528/1‑100 (MQ=255)
                                                                                             ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg   >  1:2767666/1‑100 (MQ=255)
                                                                                              cacaGCGGCATGCCCAATGAATGCGGTCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGc  <  1:1451448/100‑1 (MQ=255)
                                                                                              cacaGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAACTCCAGTACCCGGCTATTGTAACGTGTTTTTCGc  <  2:1989124/100‑1 (MQ=255)
                                                                                              cacaGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAATACCCGGCTATTGTAACGTGTTTTTCGc  >  1:1380690/1‑100 (MQ=255)
                                                                                               aCGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTCTAACGTGTTTTTCg   >  2:1769482/1‑98 (MQ=255)
                                                                                                 |                                                                                                
AGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGC  >  NC_000913/3040931‑3041124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A