Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F56 I0 R1	215	141.2	6785521	95.3%	6466601	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,289,526	A→G	64.4%	K94K (AAA→AAG)	nrfB →	nitrite reductase, formate‑dependent, penta‑heme cytochrome c

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,289,526	0	A	G	64.4%	172.3 / 153.6	166	K94K (AAA→AAG)	nrfB	nitrite reductase, formate‑dependent, penta‑heme cytochrome c
Reads supporting (aligned to +/- strand): ref base A (26/33); new base G (53/53); total (80/86)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 168 total aligned reads displayed.

TGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTG  >  NC_000913/4289427‑4289624

tGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGaaa                                                                                                    >  2:3345639/1‑100 (MQ=255)

   aaaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGGGAAGGAt                                                                                                 >  1:2671983/1‑100 (MQ=255)

    aaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtg                                                                                                <  2:2499234/100‑1 (MQ=255)

    aaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtg                                                                                                >  1:1518815/1‑100 (MQ=255)

     aaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtgt                                                                                               <  1:1567604/100‑1 (MQ=255)

      aCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtgtg                                                                                              <  1:2927624/100‑1 (MQ=255)

       cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGa                                                                                             <  1:418496/100‑1 (MQ=255)

           cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgcg                                                                                         >  2:2611560/1‑100 (MQ=255)

           cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgcg                                                                                         >  2:2531893/1‑100 (MQ=255)

           cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgcg                                                                                         >  2:1200244/1‑100 (MQ=255)

            ttCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgcgc                                                                                        >  2:599891/1‑100 (MQ=255)

               cGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCtt                                                                                      >  1:2017748/1‑99 (MQ=255)

                  tATCAACCCGAATAACAAACTGCCGGTTACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAAc                                                                                  >  2:1750256/1‑100 (MQ=255)

                     cAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAg                                                                               >  1:1822428/1‑100 (MQ=255)

                     cAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAg                                                                               <  1:2269352/100‑1 (MQ=255)

                      aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                              >  1:2193906/1‑100 (MQ=255)

                           gAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATg                                                                         <  1:374593/100‑1 (MQ=255)

                             aTAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTa                                                                       <  1:1200240/100‑1 (MQ=255)

                             aTAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTa                                                                       <  2:510823/100‑1 (MQ=255)

                              tAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTAc                                                                      <  1:3165664/100‑1 (MQ=255)

                              tAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTAc                                                                      >  1:2669328/1‑100 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGCAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                   >  2:730184/1‑100 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                   <  1:1760066/100‑1 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                   >  2:2899243/1‑100 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                   <  1:576501/100‑1 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                   >  1:2454040/1‑100 (MQ=255)

                                   aacggtcgttCAGCTGCACCTACTGGCACGGCCAGCCATCACCGCACCACCGCGAAAGGTTGAAAGATGTAATGCGCGTTAACGAGCCGATGTACAAGGt                                                                 <  1:101770/91‑1 (MQ=255)

                                   aaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                  >  1:1132181/1‑99 (MQ=255)

                                   aaCTGCCGGTCACCTGCACCAACTGTAACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGt                                                                 >  1:984009/1‑100 (MQ=255)

                                      tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTgg                                                              <  1:2798902/100‑1 (MQ=255)

                                       gCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGga                                                             <  2:986410/100‑1 (MQ=255)

                                        ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                            <  2:3171404/100‑1 (MQ=255)

                                        ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                            <  2:2433059/100‑1 (MQ=255)

                                        ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                            >  2:370618/1‑100 (MQ=255)

                                         cGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgaga                                                           <  2:1858663/100‑1 (MQ=255)

                                          gttCACCTGCACCAACTGTCACGGCAAGTCCTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTGAACGAGCCGATGTACAAGGTTGGagag                                                          <  1:2637737/98‑1 (MQ=255)

                                          ggTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCCACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGGACAAGGTTGgag                                                            >  1:1220642/1‑98 (MQ=255)

                                          ggTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                          <  2:1722694/100‑1 (MQ=255)

                                          ggTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                          >  2:2804147/1‑100 (MQ=255)

                                          ggTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                          <  2:2325910/100‑1 (MQ=255)

                                            tCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCa                                                        <  2:1453670/100‑1 (MQ=255)

                                                cTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGGTGGAGAGCAGAAc                                                    <  2:2303619/100‑1 (MQ=255)

                                                 tGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACa                                                   >  1:2330332/1‑100 (MQ=255)

                                                  gCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                  >  1:1654381/1‑100 (MQ=255)

                                                  gCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                  <  1:929061/100‑1 (MQ=255)

                                                  gCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                  <  2:3341164/100‑1 (MQ=255)

                                                   cACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                 >  2:2436348/1‑100 (MQ=255)

                                                   cACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                 >  2:1088415/1‑100 (MQ=255)

                                                   cACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                 >  2:1194273/1‑100 (MQ=255)

                                                      cAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                              <  1:1461016/100‑1 (MQ=255)

                                                      cAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                              <  1:2831050/100‑1 (MQ=255)

                                                       aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCt                                             <  1:3064239/100‑1 (MQ=255)

                                                       aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCt                                             <  1:2847285/100‑1 (MQ=255)

                                                        aCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGTGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTAAAAGGTTGGAGAGCAGAACAGCGTCTg                                            >  1:3272091/1‑100 (MQ=255)

                                                        aCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTg                                            <  2:3145905/100‑1 (MQ=255)

                                                         cTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGCGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAAAAGCGTCTg                                            >  1:594934/1‑99 (MQ=255)

                                                          tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                          <  2:401402/100‑1 (MQ=255)

                                                          tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                          <  1:978484/100‑1 (MQ=255)

                                                             cACGGCCAGCCATCACCGCAACACCGCGAAGGGGGGAAAGATGTGATGCGCTCTAACGAGCCGATGGACAAGGTTGGAGAGCAGAAACGCGGCTGTATg                                        >  1:1957003/1‑99 (MQ=255)

                                                              aCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTc                                      <  2:1920598/100‑1 (MQ=255)

                                                               cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTcc                                     >  1:2043429/1‑100 (MQ=255)

                                                               cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTcc                                     <  2:1361842/100‑1 (MQ=255)

                                                                 gccagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                   <  2:2459195/100‑1 (MQ=255)

                                                                 gccagccaTCACCGCAACACCACGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAATGATCGAGAGCAGAACAGAGTCTGTa                                          >  1:1050866/1‑93 (MQ=255)

                                                                   cagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTc                                 <  1:2139312/100‑1 (MQ=255)

                                                                   cagccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTc                                 >  2:1721898/1‑100 (MQ=255)

                                                                    agccaTCACCGCAACACCGCGAAGGGGCGAAGGATGTGATGCGCTTTAACGAGCCGTTGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCa                                >  1:1764378/1‑100 (MQ=255)

                                                                     gccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCAt                               >  1:1212234/1‑100 (MQ=255)

                                                                       caTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCt                             <  2:2669350/100‑1 (MQ=255)

                                                                       caTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCt                             >  1:2219709/1‑100 (MQ=255)

                                                                          cACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGAGGTACAAGGTAGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGcc                          >  1:2971389/1‑100 (MQ=255)

                                                                          cACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGcc                          >  1:1583858/1‑100 (MQ=255)

                                                                           aCCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCg                         >  2:2572203/1‑100 (MQ=255)

                                                                           aCCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCAGCTGcc                          <  1:2506160/99‑1 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                        >  1:3059383/1‑100 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                        >  1:319516/1‑100 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTAAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                        >  1:2067762/1‑100 (MQ=255)

                                                                             cGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGa                       <  2:208782/100‑1 (MQ=255)

                                                                             cGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGa                       <  2:49661/100‑1 (MQ=255)

                                                                              gCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGaa                      >  1:2789424/1‑100 (MQ=255)

                                                                               cAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAAc                     <  2:2164628/100‑1 (MQ=255)

                                                                                aaCACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACa                    >  1:3215820/1‑100 (MQ=255)

                                                                                  cacCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGt                  <  2:2743746/100‑1 (MQ=255)

                                                                                    ncgcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg                >  1:2952049/2‑100 (MQ=255)

                                                                                     cgcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGCCTGTATGTCCTGTCATCTGCCGGAACAGTTGc               >  1:1179866/1‑100 (MQ=255)

                                                                                      gcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCa              >  1:1423089/1‑100 (MQ=255)

                                                                                      gcgAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCa              <  2:1596526/100‑1 (MQ=255)

                                                                                      gcgAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCa              >  1:1286884/1‑100 (MQ=255)

                                                                                           ggggTGAAGGATGTAATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCGGAACAGCGTGTGTATGTCCTGTCAACTGCCGGAACAGTTGCAAAAAg         >  1:2287855/1‑100 (MQ=255)

                                                                                           ggggTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAg         <  2:3016420/100‑1 (MQ=255)

                                                                                             ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  2:2192423/1‑100 (MQ=255)

                                                                                             ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  2:1522973/1‑100 (MQ=255)

                                                                                             ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  2:2759987/1‑100 (MQ=255)

                                                                                             ggTGAAAGATGTGATGCGCTTTAACGAGCCTATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       <  1:1066090/100‑1 (MQ=255)

                                                                                             ggTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  2:2566392/1‑100 (MQ=255)

                                                                                             ggTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  1:457064/1‑100 (MQ=255)

                                                                                             ggTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg       >  2:695278/1‑100 (MQ=255)

                                                                                              gTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGt      >  2:856390/1‑100 (MQ=255)

                                                                                                gAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTc    >  1:369071/1‑100 (MQ=255)

                                                                                                  aaGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTg  >  2:2215492/1‑100 (MQ=255)

TGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTG  >  NC_000913/4289427‑4289624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A