Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F56 I0 R1	312	170.3	8276235	93.7%	7754832	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,289,526	A→G	27.7%	K94K (AAA→AAG)	nrfB →	nitrite reductase, formate‑dependent, penta‑heme cytochrome c

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,289,526	0	A	G	27.7%	329.8 / 161.5	227	K94K (AAA→AAG)	nrfB	nitrite reductase, formate‑dependent, penta‑heme cytochrome c
Reads supporting (aligned to +/- strand): ref base A (82/82); new base G (32/31); total (114/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 231 total aligned reads displayed.

TGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGG  >  NC_000913/4289427‑4289625

tGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGaaa                                                                                                     >  2:3942396/1‑100 (MQ=255)

   aaaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGAt                                                                                                  >  1:323171/1‑100 (MQ=255)

    aaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtg                                                                                                 <  2:1898282/100‑1 (MQ=255)

    aaaCATGCTTCCGTTATCAACCCGAAGAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGAtg                                                                                                 <  2:2938662/100‑1 (MQ=255)

     aaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGAtgt                                                                                                <  2:1468042/100‑1 (MQ=255)

      aCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGAtgtg                                                                                               >  1:819220/1‑100 (MQ=255)

       cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGa                                                                                              <  2:1428637/100‑1 (MQ=255)

       cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGa                                                                                              >  1:2961285/1‑100 (MQ=255)

       cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGa                                                                                              >  1:1935366/1‑100 (MQ=255)

        aTGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGa                                                                                              <  1:852915/99‑1 (MQ=255)

        aTGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGAt                                                                                             >  1:97213/1‑100 (MQ=255)

         tGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGCGAAAGATGTGATg                                                                                            >  2:174995/1‑100 (MQ=255)

           cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATgcg                                                                                          >  1:1554317/1‑100 (MQ=255)

           cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATgcg                                                                                          >  1:522522/1‑100 (MQ=255)

              ccGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCtt                                                                                       <  2:58921/100‑1 (MQ=255)

               cGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCttt                                                                                      <  2:243784/100‑1 (MQ=255)

                 gcaTCAACCCGAAGCACAAACTGCCGGTCACCTGCAGCAACGGTGGCGGCCAGCCAGCCCCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTaa                                                                                    <  2:3269913/98‑1 (MQ=255)

                  tATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAAc                                                                                   <  2:3137561/100‑1 (MQ=255)

                   aTCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACg                                                                                  >  1:475238/1‑100 (MQ=255)

                     cAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAg                                                                                >  1:2746356/1‑100 (MQ=255)

                      naCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGAGGCGCTTTAACGAg                                                                                >  1:506988/2‑99 (MQ=255)

                      aaCCTGAATAACAAACTGTCGACCACCTGCACCAACTGTCACGACCAGCCAACACCGCAACATCGCGCAGAGGCGAAAGACGTGAGAAGCTTTAACGa                                                                                 >  2:2778785/1‑98 (MQ=255)

                      aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                               <  1:2677013/100‑1 (MQ=255)

                      aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                               >  1:198114/1‑100 (MQ=255)

                      aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGc                                                                               >  2:402061/1‑100 (MQ=255)

                      aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGc                                                                               <  1:106135/100‑1 (MQ=255)

                        cccGAATAACAAAGTGCCGGTCACCTGCACCAACTGTCACGGCGAGGCATCACAGCAACAACGCGAAGGGGTGAAAGAAGTGATGCGCTTGAACGAGCCg                                                                             >  2:3780961/1‑100 (MQ=255)

                        cccGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCg                                                                             >  1:3878269/1‑100 (MQ=255)

                           gAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATg                                                                          <  1:915760/100‑1 (MQ=255)

                           gAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATg                                                                          <  1:3552210/100‑1 (MQ=255)

                           gAATAACAAACTGCCGGTCACCTGCACCAACGGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATg                                                                          <  2:1712440/100‑1 (MQ=255)

                            aaTAACAAACTGCCGTTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGTGTGAAAGATGTGATGCGCTTTAACGAGCCGATg                                                                          <  2:893761/99‑1 (MQ=255)

                              tAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTAc                                                                       >  1:3300254/1‑100 (MQ=255)

                              tAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTAc                                                                       >  2:772694/1‑100 (MQ=255)

                               aaCAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACa                                                                      <  2:964869/100‑1 (MQ=255)

                                aCAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACaa                                                                     >  1:3379287/1‑100 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                    >  1:415458/1‑100 (MQ=255)

                                 cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                    >  1:2269276/1‑100 (MQ=255)

                                  aaaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                   >  1:2142169/1‑100 (MQ=255)

                                  aaaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                   >  2:2506637/1‑100 (MQ=255)

                                  aaaCTGCCGGTCACCTGCACCAACGGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                   <  2:699420/100‑1 (MQ=255)

                                   aaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                   >  1:83556/1‑99 (MQ=255)

                                        ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                             <  2:1042810/100‑1 (MQ=255)

                                        ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                             <  1:2506651/100‑1 (MQ=255)

                                         cGGTCACCTGCACCAACTGTGACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTgg                                                               <  2:2512586/97‑1 (MQ=255)

                                          ggTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                           >  1:65896/1‑100 (MQ=255)

                                          ggTCACCGGCAGGAACTGTCACGGCGAGCCATCAGCGCCACACCGCGAAGGGGAGCAGGATGGGATGCCCTTCACCGATCCGGTGTagccgg                                                                   >  2:3387142/1‑87 (MQ=255)

                                           gTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGc                                                          <  1:2392471/100‑1 (MQ=255)

                                             cACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAg                                                        <  2:2976034/100‑1 (MQ=255)

                                             cACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAg                                                        <  1:2212441/100‑1 (MQ=255)

                                             cACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAg                                                        <  2:2876233/100‑1 (MQ=255)

                                              aCCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGa                                                       <  1:4106154/100‑1 (MQ=255)

                                               ccTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGCTGTACAAGGTTGGAGAGCAGaa                                                      >  1:3958833/1‑100 (MQ=255)

                                                  gCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                   >  2:3431631/1‑100 (MQ=255)

                                                   cACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                  >  1:3703536/1‑100 (MQ=255)

                                                     ccAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGt                                                >  2:4070334/1‑100 (MQ=255)

                                                      cAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                               <  2:203764/100‑1 (MQ=255)

                                                       aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCt                                              <  1:2643816/100‑1 (MQ=255)

                                                       aaCTATCACGGCCAGCCATCACCGCTACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAAAAGCg                                                 <  2:154956/97‑1 (MQ=255)

                                                         cttccgatctcagccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGt                                            <  2:2269531/90‑1 (MQ=255)

                                                         cTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGt                                            >  2:884991/1‑100 (MQ=255)

                                                         cTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGt                                            <  2:593429/100‑1 (MQ=255)

                                                           gTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                          >  1:1875127/1‑100 (MQ=255)

                                                             cACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAATGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGt                                        >  2:2641491/1‑100 (MQ=255)

                                                              aCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTc                                       <  1:2442414/100‑1 (MQ=255)

                                                              aCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTc                                       <  1:174401/100‑1 (MQ=255)

                                                               cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTcc                                      <  1:3408946/100‑1 (MQ=255)

                                                               cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTcc                                      <  2:3611405/100‑1 (MQ=255)

                                                                 gccagccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                    >  1:888455/1‑100 (MQ=255)

                                                                 gccagccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                    >  2:1953799/1‑100 (MQ=255)

                                                                 gccagccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                    >  1:812800/1‑100 (MQ=255)

                                                                  ccagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                    <  2:3980968/99‑1 (MQ=255)

                                                                    agccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGCCCTGTCa                                 <  1:3117141/100‑1 (MQ=255)

                                                                    agccaTCACCGCAACACCGCGAAGGGGTGAAAGATGGGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                    <  2:1311506/97‑1 (MQ=255)

                                                                      ccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATc                               <  2:217377/100‑1 (MQ=255)

                                                                         tCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGc                            >  1:3659371/1‑100 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCTTCTGTATGTCCTGTCATCTGCCgg                         <  2:1007543/100‑1 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                         <  1:772700/100‑1 (MQ=255)

                                                                            ccGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                         <  1:3488821/100‑1 (MQ=255)

                                                                             cGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGa                        <  2:451537/100‑1 (MQ=255)

                                                                             cGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGa                        <  1:1210579/100‑1 (MQ=255)

                                                                               cAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAAc                      <  2:3878244/100‑1 (MQ=255)

                                                                                aaCACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACa                     >  2:1185152/1‑100 (MQ=255)

                                                                                aaCACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACa                     <  1:1476526/100‑1 (MQ=255)

                                                                                aaCACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACa                     >  2:735152/1‑100 (MQ=255)

                                                                                 acacCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACACGGTTGGAGAGCAGAACCGCGTCTGTATGTCCTGTCATCTGCCGGAACAg                    >  1:3449769/1‑100 (MQ=255)

                                                                                 acacCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAg                    >  1:1616659/1‑100 (MQ=255)

                                                                                  cacCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGt                   >  2:2019475/1‑100 (MQ=255)

                                                                                  cacCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACa                     >  2:2081654/1‑98 (MQ=255)

                                                                                   acCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGtt                  >  1:1264474/1‑100 (MQ=255)

                                                                                    ccGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg                 >  1:1750439/1‑100 (MQ=255)

                                                                                    ccGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTAGAAGGTTGGAGAGGAGAAGAGCGTCTGTATGTCCTGTCATCTGCCGGAAAAGTTg                 >  2:229983/1‑100 (MQ=255)

                                                                                    ccGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg                 <  1:3677220/100‑1 (MQ=255)

                                                                                    ccGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg                 <  1:3825197/100‑1 (MQ=255)

                                                                                      gcgAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCa               <  2:1662754/100‑1 (MQ=255)

                                                                                            gggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGc         <  2:2441370/100‑1 (MQ=255)

                                                                                             ggTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg        >  1:2090513/1‑100 (MQ=255)

                                                                                              gTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGt       >  1:177483/1‑100 (MQ=255)

                                                                                               tGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGtt      >  1:3685835/1‑100 (MQ=255)

                                                                                               tGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGtt      <  1:1088932/100‑1 (MQ=255)

TGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGG  >  NC_000913/4289427‑4289625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A