Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,289,526 A→G 100% K94K (AAA→AAG)  nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,289,5260AG97.5% 480.9 / ‑1.1 148K94K (AAA→AAG) nrfBnitrite reductase, formate‑dependent, penta‑heme cytochrome c
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (71/72);  total (74/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTC  >  NC_000913/4289429‑4289622
                                                                                                 |                                                                                                
  aaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtg                                                                                              <  2:1327477/100‑1 (MQ=255)
   aaCATGATTCCGTTATCAACCCGAATCACAAAATGCCGATCACCTGAACCAACTTTCTCGTTCAGCGATAACCGAAACAGCGTGAAGGGGTGAAGGAtgt                                                                                             <  2:894888/100‑1 (MQ=255)
     cctGCTTCCGTTAACAACCCGAATAACAAACTGCCGGTCACCGGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGa                                                                                           >  1:2054359/3‑100 (MQ=255)
     cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGa                                                                                           >  2:306795/1‑100 (MQ=255)
     cATGCTTCCGTTATCAACCCGAATAAAAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGAtgt                                                                                             <  2:382177/98‑1 (MQ=255)
       tGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATg                                                                                         >  1:2285641/1‑100 (MQ=255)
       tGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTCCACCAACTTTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTAATg                                                                                         >  1:836383/1‑100 (MQ=255)
        gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgc                                                                                        <  2:1735190/100‑1 (MQ=255)
        gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgc                                                                                        <  2:493899/100‑1 (MQ=255)
         cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATgcg                                                                                       >  1:2302455/1‑100 (MQ=255)
           tctgTTATTAACCTGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCt                                                                                     <  1:523831/97‑1 (MQ=255)
           tCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCt                                                                                     <  1:3013521/100‑1 (MQ=255)
            ccGTTATCAACCCGAATAACAAACTGCCGGTCACCTTCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCtt                                                                                    <  1:363753/100‑1 (MQ=255)
            ccGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGGGAAGGATGTGATgc                                                                                        >  2:2717935/1‑96 (MQ=255)
             cGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGAGAAGGATGTGATGCGCttt                                                                                   >  1:258432/1‑100 (MQ=255)
                tATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGAATGTGATGCGCTTTAAc                                                                                >  1:1467437/1‑100 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCATCTGCAATAACTGTCATGGCCATCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                            <  1:1060011/100‑1 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTTTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                            <  1:284513/100‑1 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGACGGGGTGAAGGATGTGATGCGCTTTagatcgg                                                                            >  2:2411217/1‑94 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                            <  1:422156/100‑1 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                            <  1:2477403/100‑1 (MQ=255)
                    aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGc                                                                            >  1:2505951/1‑100 (MQ=255)
                     aCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGcc                                                                           >  1:488555/1‑100 (MQ=255)
                     aCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGcc                                                                           <  2:373205/100‑1 (MQ=255)
                     aCCCGAATAACAAACTGCCGGTAACCTGCACCAACTGTCACGGGCAGCCATCACCGCAACAACGCGAAGGGGTGAAGGATGTGATGCCATTTAACGAGcc                                                                           >  2:1130631/1‑100 (MQ=255)
                        cGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGCAGGGGTGAAGGCTGTGATGCGCTTTAACGAGCCTAt                                                                        >  2:2186480/1‑100 (MQ=255)
                           aTAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGTAACACCGCGAAGGGGTGAAGGATTTGATGCGCTTTAACGAGCCGATGTa                                                                     <  2:2180803/100‑1 (MQ=255)
                             aaCAAACTGCCGGTCACCTGCACCAACTTTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACa                                                                   <  1:1670514/100‑1 (MQ=255)
                             aaCAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACa                                                                   <  2:258429/100‑1 (MQ=255)
                              aCAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACaa                                                                  >  1:584396/1‑100 (MQ=255)
                              aCAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACaa                                                                  >  1:1540809/1‑100 (MQ=255)
                               cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAAGGAGCCGATGTACAAg                                                                 >  1:3002236/1‑100 (MQ=255)
                               cAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAg                                                                 <  2:79647/100‑1 (MQ=255)
                                aaaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                >  2:636037/1‑100 (MQ=255)
                                   cTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTg                                                             <  1:141758/100‑1 (MQ=255)
                                    tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTgg                                                            <  1:2763450/100‑1 (MQ=255)
                                      ccGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgag                                                          <  2:127468/100‑1 (MQ=255)
                                       cGGTCACCTGCACCAACTGTCACGGCCAGCCATCACTGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGgaga                                                         <  1:27880/100‑1 (MQ=255)
                                         gTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGc                                                       <  2:2583900/100‑1 (MQ=255)
                                         gTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGc                                                       <  2:765449/100‑1 (MQ=255)
                                           cACCTGCACCAACTGTCACGTCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATTCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAg                                                     <  2:2295826/100‑1 (MQ=255)
                                            aCCTGCACCAACTGTCTCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAg                                                     <  1:1467366/99‑1 (MQ=255)
                                            aCCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGa                                                    <  1:1633951/100‑1 (MQ=255)
                                            aCCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGa                                                    <  2:1519845/100‑1 (MQ=255)
                                             ccTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGaa                                                   >  2:1518269/1‑100 (MQ=255)
                                              cTGCACCAACTGTCACGGCCAGCCATCACCGCAACGCCGCGGAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAAc                                                  <  2:1642160/100‑1 (MQ=255)
                                              cTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTAAAGGATGTGATGCGCTTTTACGAGCCGATATAAAAGGTTGGAGAGTAGaa                                                   <  2:1420470/99‑1 (MQ=255)
                                               tGCACCAATTTTCACGGCCAGCCATCTCCTCTACACTGTGAAGGGGTGAAGTATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGa                                                    <  1:1049895/97‑1 (MQ=255)
                                                  aCCAACTGTCACGGCCAGTCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCg                                              <  2:543650/100‑1 (MQ=255)
                                                  aCCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCg                                              >  2:2729730/1‑100 (MQ=255)
                                                   ccAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGt                                             >  1:1866489/1‑100 (MQ=255)
                                                     aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCt                                           >  2:2383426/1‑100 (MQ=255)
                                                      aCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGTGTTAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGtt                                            <  2:372015/98‑2 (MQ=255)
                                                       cTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAAAAGCGTCTGt                                         >  1:958345/1‑100 (MQ=255)
                                                       cTGTCACGGCCAGCCATCACCGCAACACCGAGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGt                                         <  2:605836/100‑1 (MQ=255)
                                                        tGTCCCGGACAACCCTCACCGCAACACCGCAAAGGTGTGAAGGATGTGATGCGCTTTACCGAGCCGATGTACAAGGTTGGCGAGCAGAACTGCGtt                                            <  2:1805998/96‑2 (MQ=255)
                                                        tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                        >  2:2297257/1‑100 (MQ=255)
                                                        tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                        <  2:2141018/100‑1 (MQ=255)
                                                        tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                        <  1:691189/100‑1 (MQ=255)
                                                         gTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                       >  1:263731/1‑100 (MQ=255)
                                                         gTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                       >  1:1047752/1‑100 (MQ=255)
                                                         gTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACAAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                       >  1:866210/1‑100 (MQ=255)
                                                         gTCACGGCCAGCCATCACAGAAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTa                                        <  2:403968/99‑1 (MQ=255)
                                                          tCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATg                                      <  1:2814528/100‑1 (MQ=255)
                                                           cACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGt                                     >  1:987766/1‑100 (MQ=255)
                                                             cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGATCAGAACAGCGTCTGTATGTcc                                   <  1:561855/100‑1 (MQ=255)
                                                             cGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTcc                                   <  2:3028424/100‑1 (MQ=255)
                                                              gtccagccaTCACCGCAACACCGCGAAGGGGTAAAGGATGTGATGCGCTTTAACGAGCCTATGTACAAGGTTGGAGAGTAGAACAGCGTCTGTATGTcc                                   <  2:1207095/97‑1 (MQ=255)
                                                               gccagctaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAAGGTGCCGATGTACAAGGTTGGATAGCAGAACAGCGTCTGTATGTc                                    <  2:2301944/97‑1 (MQ=255)
                                                               gccagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTg                                 >  1:2627340/1‑100 (MQ=255)
                                                                ccagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGt                                >  2:76300/1‑100 (MQ=255)
                                                                 cagccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTc                               >  1:1959975/1‑100 (MQ=255)
                                                                  agccaTCACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCa                              <  2:1632820/100‑1 (MQ=255)
                                                                        cACCGCAACACCGCTAAGGGGTGAAGGATGTGATGCGGTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGcc                        <  1:573224/100‑1 (MQ=255)
                                                                        cACCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGcc                        <  1:636041/100‑1 (MQ=255)
                                                                         aCCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCg                       >  2:1301528/1‑100 (MQ=255)
                                                                         aCCGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTAGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCg                       >  1:942592/1‑100 (MQ=255)
                                                                          ccGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                      >  1:2410016/1‑100 (MQ=255)
                                                                          ccGCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                      <  2:1181796/100‑1 (MQ=255)
                                                                            gCAACACCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGaa                    >  1:1498816/1‑100 (MQ=255)
                                                                            gCAACACCGCGAAGGGGTGAAGGAAGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACATCGTCTGGATATCATGTCATCTGcc                        >  2:1053094/1‑96 (MQ=255)
                                                                               acacCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAg                 >  2:1138036/1‑100 (MQ=255)
                                                                                cacCGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGt                >  1:2259214/1‑100 (MQ=255)
                                                                                 acCGCGAAGGGGTGAAGGATGTGATGTGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                      <  2:1866883/93‑1 (MQ=255)
                                                                                  ccGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg              >  1:483923/1‑100 (MQ=255)
                                                                                  ccGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg              >  2:2083006/1‑100 (MQ=255)
                                                                                  ccGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg              >  1:2275825/1‑100 (MQ=255)
                                                                                  ccGCGAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTg              >  1:2014431/1‑100 (MQ=255)
                                                                                   cgcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGc             <  2:958339/100‑1 (MQ=255)
                                                                                   cgcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGc             <  2:2014408/100‑1 (MQ=255)
                                                                                   cgcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGc             <  1:2368217/100‑1 (MQ=255)
                                                                                    gcgAAGGGGTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCa            <  1:2562835/100‑1 (MQ=255)
                                                                                         ggggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAg       >  2:1965469/1‑100 (MQ=255)
                                                                                           ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg     >  2:1747206/1‑100 (MQ=255)
                                                                                           ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg     >  2:915694/1‑100 (MQ=255)
                                                                                           ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCATGTCATCTGCCGGAACAGTTGCAAAAAGCg     >  1:2684008/1‑100 (MQ=255)
                                                                                           ggTGAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAAAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg     >  1:925860/1‑100 (MQ=255)
                                                                                              gAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTc  >  1:415641/1‑100 (MQ=255)
                                                                                              gAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTc  >  1:1429659/1‑100 (MQ=255)
                                                                                              gAAGGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTc  >  1:339062/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTC  >  NC_000913/4289429‑4289622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A