Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F56 I0 R1	215	141.2	6785521	95.3%	6466601	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,560,045	C→A	22.8%	W212C (TGG→TGT)	yjiH ←	nucleoside recognition pore and gate family putative inner membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,045	0	C	A	22.8%	339.4 / 97.5	162	W212C (TGG→TGT)	yjiH	nucleoside recognition pore and gate family putative inner membrane transporter
Reads supporting (aligned to +/- strand): ref base C (64/61); new base A (15/22); total (79/83)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 168 total aligned reads displayed.

GGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCG  >  NC_000913/4559946‑4560144

ggCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTTGAAGTTAAGc                                                                                                     <  1:2385789/100‑1 (MQ=255)

 gcgcgcgcCATCGATAAACATATCCATGACGTTTTTGCTGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGcc                                                                                                    <  1:1627310/100‑1 (MQ=255)

 gcgcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTCCGAAGTTAAGcc                                                                                                    <  2:2234881/100‑1 (MQ=255)

    cgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGa                                                                                                 <  2:108705/100‑1 (MQ=255)

      cgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGAcg                                                                                               >  2:2065021/1‑100 (MQ=255)

        ccATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGAcgcg                                                                                             >  1:2767422/1‑100 (MQ=255)

         cATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACgcgc                                                                                            >  1:311130/1‑100 (MQ=255)

           tCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCaa                                                                                          <  1:3081417/100‑1 (MQ=255)

               tAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATg                                                                                      <  1:746472/100‑1 (MQ=255)

                aaaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGt                                                                                     <  1:164557/100‑1 (MQ=255)

                 aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGtt                                                                                    >  2:293525/1‑100 (MQ=255)

                 aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGtt                                                                                    <  1:110710/100‑1 (MQ=255)

                 aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGtt                                                                                    >  1:2004480/1‑100 (MQ=255)

                  aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                                                                                   <  1:1851656/100‑1 (MQ=255)

                  aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                                                                                   <  2:1607345/100‑1 (MQ=255)

                  aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                                                                                   <  1:1327844/100‑1 (MQ=255)

                  aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                                                                                   <  2:2853450/100‑1 (MQ=255)

                    atatCCATGACGTTTTTGCGGACCTGAGTTGTCATGCGTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGc                                                                                 <  1:599385/100‑1 (MQ=255)

                      atCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTc                                                                               <  2:813545/100‑1 (MQ=255)

                       tCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTcc                                                                              >  2:676515/1‑100 (MQ=255)

                       tCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTcc                                                                              <  1:730754/100‑1 (MQ=255)

                        ccATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCg                                                                             >  2:752706/1‑100 (MQ=255)

                          aTGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGAc                                                                           <  2:2861612/100‑1 (MQ=255)

                              cGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAAt                                                                       <  1:1061727/100‑1 (MQ=255)

                               gTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAAtt                                                                      >  1:406286/1‑100 (MQ=255)

                                  tttGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTaa                                                                   <  1:1052901/100‑1 (MQ=255)

                                   ttGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTaaa                                                                  >  2:1050704/1‑100 (MQ=255)

                                   ttGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTaaa                                                                  >  1:3127135/1‑100 (MQ=255)

                                   ttGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGAGACGTTCTTCGAAGTTAAGCAAGACGCGCAAAATGTTGGCTCCGACAAATTCaaa                                                                  >  1:2968003/1‑100 (MQ=255)

                                    tGCGGACCTGATTTGTCATGGTTGCGCGCCTTGCGTTGGTTTGCGACGTTTTTCGAAGTTATGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAac                                                                 <  2:1974215/100‑1 (MQ=255)

                                    tGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAac                                                                 <  2:752575/100‑1 (MQ=255)

                                    tGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAac                                                                 <  1:3164504/100‑1 (MQ=255)

                                    tGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAac                                                                 <  1:2639801/100‑1 (MQ=255)

                                     gCGGACCTGAGTTGTCATGCTTGTGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAACATGTTGGCTCCGACAAATTTAAaca                                                                >  1:232995/1‑100 (MQ=255)

                                      cGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAacac                                                               >  2:1680831/1‑100 (MQ=255)

                                      cGGACCTGAGTTGTCATACTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAacac                                                               >  1:3028237/1‑100 (MQ=255)

                                        gACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCa                                                             >  2:1765775/1‑100 (MQ=255)

                                         aCCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAg                                                            <  2:1315335/100‑1 (MQ=255)

                                         aCCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAg                                                            <  2:2767445/100‑1 (MQ=255)

                                         aCCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAg                                                            <  2:683301/100‑1 (MQ=255)

                                          ccTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAgg                                                           >  1:1139312/1‑100 (MQ=255)

                                           cTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGa                                                          <  2:668516/100‑1 (MQ=255)

                                            tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGAt                                                         >  2:3109524/1‑100 (MQ=255)

                                             gAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATg                                                        <  2:3365198/100‑1 (MQ=255)

                                             gAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATg                                                        >  2:2579613/1‑100 (MQ=255)

                                                ttGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACg                                                     >  1:1567408/1‑100 (MQ=255)

                                                ttGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACg                                                     <  2:71724/100‑1 (MQ=255)

                                                ttGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACg                                                     <  2:2974080/100‑1 (MQ=255)

                                                 tGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACgg                                                    <  1:3072847/100‑1 (MQ=255)

                                                  gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGACGTTACGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   >  1:1119108/1‑100 (MQ=255)

                                                  gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   <  2:250809/100‑1 (MQ=255)

                                                  gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   >  2:2755805/1‑100 (MQ=255)

                                                      tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               >  2:2881648/1‑100 (MQ=255)

                                                       gCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAg                                              <  1:3328137/100‑1 (MQ=255)

                                                        cTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAgg                                             >  1:1523192/1‑100 (MQ=255)

                                                         ttGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGt                                            >  2:505528/1‑100 (MQ=255)

                                                          tGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTa                                           <  2:1109281/100‑1 (MQ=255)

                                                           gcgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTAc                                          >  1:2957646/1‑100 (MQ=255)

                                                              cTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAt                                       <  1:2546279/100‑1 (MQ=255)

                                                               tCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAtt                                      <  1:1164888/100‑1 (MQ=255)

                                                                ccTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTa                                     >  2:1870220/1‑100 (MQ=255)

                                                                 cTTGCGTTGGGTTGCGACGTTCTTCGAAGTTACGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTAc                                    >  1:2187721/1‑100 (MQ=255)

                                                                 cTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTAc                                    >  1:1928552/1‑100 (MQ=255)

                                                                   tGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCTGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACgg                                  >  1:799517/1‑100 (MQ=255)

                                                                    gCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGa                                 <  1:2494794/100‑1 (MQ=255)

                                                                        tGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTg                             <  2:325045/100‑1 (MQ=255)

                                                                           gTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACCAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          >  1:1534740/1‑100 (MQ=255)

                                                                           gTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACCCCAGGATGACGGCTAAAGGTACGATTACGGACGTGcc                           <  1:2250015/99‑1 (MQ=255)

                                                                           gTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          <  2:628667/100‑1 (MQ=255)

                                                                           gTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          >  1:636865/1‑100 (MQ=255)

                                                                             tGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAg                        <  2:874534/100‑1 (MQ=255)

                                                                               cGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGaa                      >  2:1075489/1‑100 (MQ=255)

                                                                               cGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGaa                      >  1:1722359/1‑100 (MQ=255)

                                                                               cGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGaa                      <  2:1521972/100‑1 (MQ=255)

                                                                                gACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGAAGGCTAAAGGTACGATTACGGACGTGCCCAGaaa                     <  1:835000/100‑1 (MQ=255)

                                                                                 aCGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAg                    <  2:2670129/100‑1 (MQ=255)

                                                                                 aCGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACGAGGATGACGGCGAAAGGTACGATTACGGACGTGCCCAGAAAg                    <  1:442357/100‑1 (MQ=255)

                                                                                  cGTTCTTCGAAGTTAAGCCAGACGCGCATAATGTTGGCTCCGACAAATTTAAACACCAGGATGAATGGTAAAGGTACGATTACGGACGTGCCCAGAAAg                    <  1:2032166/99‑1 (MQ=255)

                                                                                  cGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAgg                   <  1:1494934/100‑1 (MQ=255)

                                                                                   gTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGc                  <  2:2444927/100‑1 (MQ=255)

                                                                                   gTTCGTCGAAGTTAAGCCAGCCGCGCAAATTGTTGTCCCCGACAAATTTAATGACCAGGAGGACGGCTAAAGGTACGATTACGGACGGGCCCAGACAg                    <  1:980314/98‑1 (MQ=255)

                                                                                    ttcttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCa                 <  2:3028263/100‑1 (MQ=255)

                                                                                    ttcttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCa                 <  1:1793819/100‑1 (MQ=255)

                                                                                     tcttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCaa                <  1:588643/100‑1 (MQ=255)

                                                                                      cttcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCaaa               >  2:2559935/1‑100 (MQ=255)

                                                                                      cttcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCaaa               >  1:2463213/1‑100 (MQ=255)

                                                                                       ttcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAac              <  2:436396/100‑1 (MQ=255)

                                                                                       ttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACCCCAGGATGACGGCTAAAGGTACGATTTCGGACGTGCCCAGAAAGGCAAac              <  1:2586196/100‑1 (MQ=255)

                                                                                           aaGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcg          <  2:1625433/100‑1 (MQ=255)

                                                                                            aGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc         >  2:96400/1‑100 (MQ=255)

                                                                                            aGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTCAAACACCAGGATTACGGCTCAAGTTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc         >  1:664870/1‑100 (MQ=255)

                                                                                             gTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCATAAAGGCAAACACcgcg        >  1:1976728/1‑100 (MQ=255)

                                                                                             gTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgcg        >  2:135767/1‑100 (MQ=255)

                                                                                             gTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgcg        >  1:995454/1‑100 (MQ=255)

                                                                                             gTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgcg        <  2:312425/100‑1 (MQ=255)

                                                                                               tAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGAc      >  2:1364565/1‑100 (MQ=255)

                                                                                               tAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGAc      >  2:2896914/1‑100 (MQ=255)

                                                                                                  gCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGcc   >  2:1214087/1‑100 (MQ=255)

                                                                                                  gCCAGACGCGCAAAATGTTGGCTCCGACAAATATAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGcc   >  1:1324491/1‑100 (MQ=255)

                                                                                                   ccAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAACGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCg  <  2:1281329/100‑1 (MQ=255)

GGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCG  >  NC_000913/4559946‑4560144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A