Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I1 R1 155 65.2 3164312 95.9% 3034575 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,638,037 C→A A206S (GCG→TCG)  bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,0370CA100.0% 186.9 / NA 56A206S (GCG→TCG) bamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/25);  total (31/25)

AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTC  >  NC_000913/2637941‑2638131
                                                                                                |                                                                                              
aaCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAAcc                                                                                             <  2:15760/100‑1 (MQ=255)
    ggTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCaaaa                                                                                         <  1:1507693/100‑1 (MQ=255)
          cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACGAAAAGCCGt                                                                                    >  1:406072/1‑99 (MQ=255)
              gAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCg                                                                               >  2:84471/1‑100 (MQ=255)
                aaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgc                                                                             <  1:178542/100‑1 (MQ=255)
                  tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcgc                                                                           <  2:184372/100‑1 (MQ=255)
                   aCGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCa                                                                          >  2:292935/1‑100 (MQ=255)
                    cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAg                                                                         >  1:1487702/1‑100 (MQ=255)
                      ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGAc                                                                       <  2:155074/100‑1 (MQ=255)
                           gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                  <  2:897262/100‑1 (MQ=255)
                           gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                  >  2:253174/1‑100 (MQ=255)
                             cAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCAc                                                                >  2:1307899/1‑100 (MQ=255)
                              aaaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACTAAAAGCCGTTGTCGGCGCAGACTCGCCACg                                                               <  2:613045/100‑1 (MQ=255)
                               aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                              <  1:235959/100‑1 (MQ=255)
                                atcatcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                                             <  2:587080/100‑1 (MQ=255)
                                   atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                          <  2:1266042/100‑1 (MQ=255)
                                   atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                          >  1:1422565/1‑100 (MQ=255)
                                     cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                        <  1:1384406/100‑1 (MQ=255)
                                      tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCTCGACAaga                                                         >  2:555509/1‑98 (MQ=255)
                                      tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                       >  2:531102/1‑100 (MQ=255)
                                      tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTAGGCGCAGACTCGCCACGCaa                                                            >  2:110070/1‑95 (MQ=255)
                                       ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCGCGCAAagagag                                                      <  2:714319/100‑1 (MQ=255)
                                       ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                      >  2:1047478/1‑100 (MQ=255)
                                            tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCACCACGCAAAGAGAGCGAAg                                                 <  1:160151/100‑1 (MQ=255)
                                             gTTCCAGCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAgg                                                <  2:820595/100‑1 (MQ=255)
                                               tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTATCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                              <  1:1117889/100‑1 (MQ=255)
                                                caaTCAGCACTGCGCTGACGCGACCATTATCGACCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCat                                             >  2:919251/3‑100 (MQ=255)
                                                 cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                            >  2:27793/1‑100 (MQ=255)
                                                  aTCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCatat                                           >  1:145857/1‑100 (MQ=255)
                                                  aTCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCatat                                           >  1:460362/1‑100 (MQ=255)
                                                    cAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCg                                         >  2:128645/1‑100 (MQ=255)
                                                     aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGa                                        >  1:442959/1‑100 (MQ=255)
                                                      gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                       >  1:162262/1‑100 (MQ=255)
                                                      gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                       <  1:292935/100‑1 (MQ=255)
                                                         cTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                    >  1:1539455/1‑100 (MQ=255)
                                                          tGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTa                                   >  1:264149/1‑100 (MQ=255)
                                                           gcgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTaa                                  >  2:470323/1‑100 (MQ=255)
                                                            cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                 <  1:1238070/100‑1 (MQ=255)
                                                            cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                 <  1:1307905/100‑1 (MQ=255)
                                                                  cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTcc                            <  2:157329/99‑1 (MQ=255)
                                                                  cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                           >  1:996446/1‑100 (MQ=255)
                                                                  cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                           >  1:490825/1‑100 (MQ=255)
                                                                    cgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCCAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAtt                         >  2:4655/1‑100 (MQ=255)
                                                                      aCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                       >  1:271131/1‑100 (MQ=255)
                                                                       ccATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGa                      >  1:1383722/1‑100 (MQ=255)
                                                                        cATTATCGCCCCCCACGACGGACGAACCAAAAGCCGTTGTCGGCGCCGACTCGCCACGCAAAGAGCGCGAAGGCATATCGAGGTGAACTGTCAAttt                        >  2:1290089/1‑97 (MQ=255)
                                                                           tATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgc                  >  2:821645/1‑100 (MQ=255)
                                                                                 cccccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTc            <  1:84471/100‑1 (MQ=255)
                                                                                  ccccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCa           >  1:308322/1‑100 (MQ=255)
                                                                                    ccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  1:519385/100‑1 (MQ=255)
                                                                                    ccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  1:315884/100‑1 (MQ=255)
                                                                                     cACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  2:1138259/99‑1 (MQ=255)
                                                                                     cACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt        >  1:1480901/1‑100 (MQ=255)
                                                                                       cgacgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTc      >  1:669729/1‑100 (MQ=255)
                                                                                         acgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGt    <  2:490824/100‑1 (MQ=255)
                                                                                           ggCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTc  <  2:631173/100‑1 (MQ=255)
                                                                                                |                                                                                              
AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTC  >  NC_000913/2637941‑2638131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A