Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F56 I1 R1
|
155 |
65.2 |
3164312 |
95.9% |
3034575 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,443,086 |
T→A |
N338Y (AAC→TAC) |
secY ← |
preprotein translocase membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,443,086 | 0 | T | A | 94.1%
| 221.7
/ 2.5
| 68 | N338Y (AAC→TAC) | secY | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base A (30/34); total (31/37) |
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT > NC_000913/3442990‑3443182
|
ttACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGaa > 1:847126/1‑100 (MQ=255)
aTCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAAc > 2:1491771/1‑100 (MQ=255)
tCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACg < 1:422042/100‑1 (MQ=255)
ataCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtg < 1:733739/100‑1 (MQ=255)
aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTa < 2:1411878/100‑1 (MQ=255)
aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa > 1:506433/1‑100 (MQ=255)
aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa < 1:324680/100‑1 (MQ=255)
aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATACACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa > 2:861573/1‑100 (MQ=255)
cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag > 2:398997/1‑100 (MQ=255)
cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag < 2:610001/100‑1 (MQ=255)
cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag < 2:1367867/100‑1 (MQ=255)
cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag > 2:1503788/1‑100 (MQ=255)
cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag < 2:981475/100‑1 (MQ=255)
gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga < 2:1552817/100‑1 (MQ=255)
gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga < 1:1322003/100‑1 (MQ=255)
ccGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAgaagaa < 2:1366936/100‑1 (MQ=255)
tttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACa < 1:850797/100‑1 (MQ=255)
ttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACag < 2:161450/100‑1 (MQ=255)
gCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaa > 2:416624/1‑100 (MQ=255)
ctctCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaag < 2:625807/100‑1 (MQ=255)
tcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaag > 1:160951/1‑100 (MQ=255)
tcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaag > 1:205333/1‑100 (MQ=255)
cccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaaga > 1:777208/1‑100 (MQ=255)
gACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat < 1:901013/100‑1 (MQ=255)
cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg > 1:1251082/1‑100 (MQ=255)
gAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGc < 1:1270240/100‑1 (MQ=255)
aaTTCCTGGTACAAATGCACCGGATTTCTTCAGGTTATCTGCTTTGTCACGCGGATAGAAAACCAACTCCTTGTAGAAGAAACAGAAGAAGATGATTGc < 2:318823/99‑1 (MQ=255)
aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCa < 1:878073/100‑1 (MQ=255)
aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCa < 1:877969/100‑1 (MQ=255)
aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg < 2:930718/100‑1 (MQ=255)
aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg < 1:192768/100‑1 (MQ=255)
cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc < 1:1413658/100‑1 (MQ=255)
cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc > 2:1453511/1‑100 (MQ=255)
tGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCAGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCa < 1:1503797/100‑1 (MQ=255)
aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa > 2:712456/1‑100 (MQ=255)
tGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAac < 2:526249/100‑1 (MQ=255)
tGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAac < 1:1362837/100‑1 (MQ=255)
cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac < 1:1491780/100‑1 (MQ=255)
cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac < 1:1080233/100‑1 (MQ=255)
cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac < 1:218179/100‑1 (MQ=255)
aCCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca < 2:160951/100‑1 (MQ=255)
ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca > 2:1110033/1‑99 (MQ=255)
ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca > 2:1494461/1‑99 (MQ=255)
ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt > 1:458188/1‑100 (MQ=255)
ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt > 1:380832/1‑100 (MQ=255)
aCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAg > 1:141581/1‑100 (MQ=255)
cttcttCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGc > 1:435588/1‑100 (MQ=255)
tcttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAATACATAAAGCgg < 2:1226569/100‑1 (MQ=255)
tcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGGAGAAGAATCAGGAGAAGGTGATTACAGACGGAAAGAGTAACACATAAAACGGtt > 2:1065984/1‑99 (MQ=255)
cAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc > 2:911375/1‑100 (MQ=255)
cAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATAATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc > 1:1108627/1‑100 (MQ=255)
aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc < 1:1347816/100‑1 (MQ=255)
aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc > 1:753543/1‑100 (MQ=255)
ttATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg < 2:806523/100‑1 (MQ=255)
tATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAgg > 1:1506028/1‑100 (MQ=255)
ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg < 2:1157411/100‑1 (MQ=255)
ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATAGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg > 2:1194320/1‑100 (MQ=255)
tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc > 2:1474426/1‑100 (MQ=255)
tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc > 1:906961/1‑100 (MQ=255)
tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc > 1:152312/1‑100 (MQ=255)
gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa > 2:1533480/1‑100 (MQ=255)
ctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaa > 2:796163/1‑100 (MQ=255)
tttttCAAGCGGGTAAAAAAACAACAGCGTGTAAAAAAAACAGAAAAAGATGATTTCAGACGCATAGATTAACACATAAAGCGGTTTCCCAGGCTTCaaa < 2:998445/98‑1 (MQ=255)
tgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaaa < 2:1479751/100‑1 (MQ=255)
tttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAAt < 2:506432/99‑1 (MQ=255)
cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCGTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAg < 1:727497/100‑1 (MQ=255)
cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg > 1:140049/1‑100 (MQ=255)
ggTTTAAAACCAACGCCGTGTAGAATAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAt < 2:1473008/100‑1 (MQ=255)
|
TTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT > NC_000913/3442990‑3443182
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
