Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I1 R1 155 65.2 3164312 95.9% 3034575 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,547,319 C→A R742S (CGT→AGT)  fimD → fimbrial usher outer membrane porin protein; FimCD chaperone‑usher

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,547,3190CA98.4% 210.6 / ‑3.4 62R742S (CGT→AGT) fimDfimbrial usher outer membrane porin protein; FimCD chaperone‑usher
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (24/37);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

TGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATA  >  NC_000913/4547224‑4547412
                                                                                               |                                                                                             
tGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTgg                                                                                           <  1:822601/100‑1 (MQ=255)
 ggggCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGt                                                                                          <  1:1193776/100‑1 (MQ=255)
 ggggCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGt                                                                                          >  1:1314246/1‑100 (MQ=255)
 ggggCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGt                                                                                          <  1:243520/100‑1 (MQ=255)
      aGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGc                                                                                     <  1:298806/100‑1 (MQ=255)
          gTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGtg                                                                                 >  1:1380810/1‑100 (MQ=255)
             aaaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGtgctg                                                                              <  2:1578241/100‑1 (MQ=255)
             aaaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGtgctg                                                                              >  1:967944/1‑100 (MQ=255)
              aaCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGtgctgc                                                                             <  1:547490/100‑1 (MQ=255)
               aCGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGcc                                                                            <  2:1210938/100‑1 (MQ=255)
                cGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCt                                                                           >  1:505232/1‑100 (MQ=255)
                   tACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTAt                                                                        <  1:600215/100‑1 (MQ=255)
                   tACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTAt                                                                        <  1:36233/100‑1 (MQ=255)
                    aCGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATg                                                                       <  1:761957/100‑1 (MQ=255)
                    aCGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATg                                                                       <  1:661086/100‑1 (MQ=255)
                     cGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGc                                                                      >  1:308425/1‑100 (MQ=255)
                     cGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGc                                                                      <  2:657681/100‑1 (MQ=255)
                           tgCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGa                                                                <  2:533126/100‑1 (MQ=255)
                            gCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGaa                                                               >  2:1007606/1‑100 (MQ=255)
                            gCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGaa                                                               >  1:54811/1‑100 (MQ=255)
                             cTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAat                                                              <  2:1302380/100‑1 (MQ=255)
                               tGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAatat                                                            >  1:368864/1‑100 (MQ=255)
                               tGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAatat                                                            >  2:391017/1‑100 (MQ=255)
                                gTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATc                                                           >  2:379861/1‑100 (MQ=255)
                                  tAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCgg                                                         <  2:1563994/100‑1 (MQ=255)
                                  tAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCgg                                                         <  1:944351/100‑1 (MQ=255)
                                  tAAAGCGCCTGGAGCAAAAGATGAAAAAGTCGAAACCCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCgg                                                         <  2:981798/100‑1 (MQ=255)
                                  gaaaGCTCCTGGCGCAAAAGTTGGAAAGTGCTAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCgg                                                         <  1:1081964/99‑1 (MQ=255)
                                   aaaGCGCCTGGCGCAAAAGATGTAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACCGAATATCggg                                                        >  2:608476/1‑100 (MQ=255)
                                   aaaGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCggg                                                        >  1:128562/1‑100 (MQ=255)
                                    aaGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAatattg                                                          >  2:21690/1‑95 (MQ=255)
                                     aGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGaa                                                      <  1:528833/100‑1 (MQ=255)
                                          cTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATag                                                 >  1:1003307/1‑100 (MQ=255)
                                           tGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAAt                                                   <  2:1482632/97‑1 (MQ=255)
                                            ggCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATagag                                               <  2:1003301/100‑1 (MQ=255)
                                            ggCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATagag                                               >  1:596903/1‑100 (MQ=255)
                                            ggCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATagag                                               >  2:773602/1‑100 (MQ=255)
                                            ggCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATagag                                               <  2:1360814/100‑1 (MQ=255)
                                             gcgcAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGt                                              <  1:1552650/100‑1 (MQ=255)
                                              cgcAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTg                                             >  1:1228709/1‑100 (MQ=255)
                                               gcAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTgg                                            >  1:1149061/1‑100 (MQ=255)
                                                 aaaaGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGgcg                                          >  1:1481096/1‑100 (MQ=255)
                                                  aaaGATGCAAAAGTCGAAAACCAGACGTGGGTGCGTACCGACTGGAGTGGTTATGCCGTGATGCCTTATGCCACTGAATATTGGGAAAATAGTGTGgtgc                                         >  2:1158927/1‑100 (MQ=255)
                                                   aaGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCt                                        <  1:1367468/100‑1 (MQ=255)
                                                     gATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCAACTGAATATCGGGAAAATAGAGTGGCGCTgg                                      <  2:1051725/100‑1 (MQ=255)
                                                        gCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATa                                   <  2:383667/100‑1 (MQ=255)
                                                         cAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATAc                                  >  1:75802/1‑100 (MQ=255)
                                                          aaaaGTCGAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATAcc                                 <  1:601504/100‑1 (MQ=255)
                                                           aaaGTCGAAAACCAGACGGAGGTGCGTACCGACTGGAGTGGTTATGCCGCGAAGCCTTATGCAAATGACTATCGGGAAAATAGAGTGACGCTGGATACCa                                <  2:707311/100‑1 (MQ=255)
                                                                ggAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATa                             >  2:1314720/2‑98 (MQ=255)
                                                                 gAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATAccc                          >  1:1105121/1‑100 (MQ=255)
                                                                 gAAAACCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATAccc                          >  2:1226853/1‑100 (MQ=255)
                                                                   aaaCCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTg                        >  2:443541/1‑100 (MQ=255)
                                                                    aaCCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTgg                       <  1:379861/100‑1 (MQ=255)
                                                                     aCCAGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGc                      <  2:709871/100‑1 (MQ=255)
                                                                        aGACGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGa                   <  1:1093549/100‑1 (MQ=255)
                                                                          aCGGGGGTGCGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATa                 <  2:1002332/100‑1 (MQ=255)
                                                                                   cGTACCGACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGAtt        <  1:1576546/100‑1 (MQ=255)
                                                                                      aCCGACTGGAGTGGTTCTGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAg     <  2:927362/100‑1 (MQ=255)
                                                                                         gACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATa  <  1:391017/100‑1 (MQ=255)
                                                                                         gACTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATa  <  2:94867/100‑1 (MQ=255)
                                                                                          aCTGGAGTGGTTATGCCGTGCTGCCTTATGCCACTGATTATCGGGACAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGAttcaaat   <  2:450540/98‑6 (MQ=255)
                                                                                               |                                                                                             
TGGGGCAGCCGTTAAACGATACGGTGGTGCTTGTTAAAGCGCCTGGCGCAAAAGATGCAAAAGTCGAAAACCAGACGGGGGTGCGTACCGACTGGCGTGGTTATGCCGTGCTGCCTTATGCCACTGAATATCGGGAAAATAGAGTGGCGCTGGATACCAATACCCTGGCTGATAACGTCGATTTAGATA  >  NC_000913/4547224‑4547412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A