Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
343,467 |
A→C |
H195P (CAC→CCC) |
yahK → |
broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 343,467 | 0 | A | C | 95.5%
| 151.4
/ ‑3.7
| 44 | H195P (CAC→CCC) | yahK | broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (21/21); total (22/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACG > NC_000913/343373‑343560
|
tATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGa > 2:301365/1‑100 (MQ=255)
aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGAt < 2:208638/100‑1 (MQ=255)
cgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGc < 2:808005/100‑1 (MQ=255)
cGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACAtg > 1:553565/1‑100 (MQ=255)
gTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACAtgt > 2:456918/1‑100 (MQ=255)
cACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGCGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGTGGCGCATgtgg < 2:827094/100‑1 (MQ=255)
tGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGtggtgg < 1:118904/100‑1 (MQ=255)
gCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCa < 2:274017/100‑1 (MQ=255)
cAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCAt > 1:1119666/1‑100 (MQ=255)
cAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCAt > 2:137985/1‑100 (MQ=255)
gccgggccggGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTa < 2:339613/100‑1 (MQ=255)
cgggccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTAcc < 1:389606/100‑1 (MQ=255)
aaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACACGTGGTGGCATTTACCACTTCTGAGGCaa > 2:1136115/1‑100 (MQ=255)
aaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCaaa < 2:1539392/100‑1 (MQ=255)
tGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTACGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAAcg < 1:292875/100‑1 (MQ=255)
gCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGa < 1:456920/100‑1 (MQ=255)
gCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGa < 2:590377/100‑1 (MQ=255)
tGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGc > 1:840288/1‑100 (MQ=255)
gTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCgg > 1:1004170/1‑100 (MQ=255)
cGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCa > 1:1371394/1‑100 (MQ=255)
ggCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaa > 1:1490692/1‑100 (MQ=255)
gCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaa > 2:607002/1‑100 (MQ=255)
cATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa > 1:1125152/1‑100 (MQ=255)
cATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa > 2:679033/1‑100 (MQ=255)
tCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGc > 2:365084/1‑100 (MQ=255)
ggTCTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTgg < 2:477262/100‑1 (MQ=255)
cTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAACAGCCCTggggg > 2:713943/1‑100 (MQ=255)
cTGGGACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTggggg > 1:494812/1‑100 (MQ=255)
gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:454227/100‑1 (MQ=255)
gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:454215/100‑1 (MQ=255)
gggACATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGcc < 1:254849/100‑1 (MQ=255)
cATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg < 2:847385/100‑1 (MQ=255)
cATATGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg < 2:893714/100‑1 (MQ=255)
atGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAg < 1:607005/100‑1 (MQ=255)
atGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAg < 1:1339752/100‑1 (MQ=255)
tGGGGATTAAGCTGGCTCACGGGGTGGGGGGACATGTGGTGGCGTTTACAACTTCTGAGGCAAAACACGAAGCGGGAAAAGGCCGGGGGGCCGAtcgag > 2:151235/1‑95 (MQ=255)
tGGGGATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgt > 2:479746/1‑100 (MQ=255)
ggATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgt > 1:1274127/1‑100 (MQ=255)
gATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgtt < 2:1180096/100‑1 (MQ=255)
gATTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttgt < 2:1175213/99‑1 (MQ=255)
aTTAAGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTa > 1:1320032/1‑100 (MQ=255)
aGCTGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTc > 1:420690/1‑100 (MQ=255)
cTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCAc < 2:175104/100‑1 (MQ=255)
tGGCCCCCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACg > 2:329593/1‑100 (MQ=255)
|
TATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACG > NC_000913/343373‑343560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
