Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,728,444 |
C→A |
T33N (ACC→AAC) |
rnt → |
RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,728,444 | 0 | C | A | 97.0%
| 229.6
/ NA
| 66 | T33N (ACC→AAC) | rnt | RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (33/31); total (33/33) |
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGT > NC_000913/1728347‑1728543
|
aTGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTTGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACg < 2:1426367/100‑1 (MQ=255)
aTGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACg < 2:1219930/100‑1 (MQ=255)
aTGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACg < 2:665187/100‑1 (MQ=255)
tGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGa < 2:48854/100‑1 (MQ=255)
tCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATg > 1:634712/1‑100 (MQ=255)
cGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATgcg > 1:564108/1‑100 (MQ=255)
gATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATgcgc < 2:1439956/100‑1 (MQ=255)
tAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCgctg > 1:1089822/1‑100 (MQ=255)
aCGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACTCCAAAAACGATGCgctgct < 2:157265/100‑1 (MQ=255)
cGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCtt > 1:1152505/1‑100 (MQ=255)
cTCAACTTACCGGTCTGTGTGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga < 1:1304726/100‑1 (MQ=255)
cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga < 1:1238826/100‑1 (MQ=255)
cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga > 1:1048197/1‑100 (MQ=255)
cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga > 2:370683/1‑100 (MQ=255)
cAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgaga > 1:1006972/1‑100 (MQ=255)
ggTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGTAACAGCTGGATAAAACAAAAAAAACGATGCGCTGCTTGAGATTGCCGCCa < 2:277999/100‑1 (MQ=255)
ggTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGGGATTGCCGCCa > 2:740693/1‑100 (MQ=255)
ggTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCa > 2:740707/1‑100 (MQ=255)
gtgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCAcc > 1:704882/1‑100 (MQ=255)
gCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCt < 1:1539303/100‑1 (MQ=255)
gCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCt < 1:418488/100‑1 (MQ=255)
cGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTg > 2:495263/1‑100 (MQ=255)
cGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTg > 1:768109/1‑100 (MQ=255)
cGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaaa > 2:979047/1‑100 (MQ=255)
gTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAAt > 1:1465803/1‑100 (MQ=255)
tttCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATgg < 1:1364198/100‑1 (MQ=255)
cGTGGTGTTTATCCTTGGGTAAACGATAGTGAAACATCCGGATTCAACGCCAAAACCGATGCGCTGCTTGAAATTGCCGCCATCACCCTGAAAATGGa < 2:1229237/98‑1 (MQ=255)
gTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTCACGCCGAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATg > 2:307935/1‑99 (MQ=255)
gTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGa > 1:176778/1‑100 (MQ=255)
tGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGaa < 1:20705/100‑1 (MQ=255)
ggTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAAc > 2:818220/1‑100 (MQ=255)
gTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACa < 1:713615/100‑1 (MQ=255)
tttttATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACaa > 1:1177628/1‑100 (MQ=255)
ttttATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAg > 1:212257/1‑100 (MQ=255)
aTCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctg < 2:564105/100‑1 (MQ=255)
cTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggct > 1:538056/1‑100 (MQ=255)
cTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggct > 2:517057/1‑100 (MQ=255)
cTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggct < 1:891228/100‑1 (MQ=255)
tgttgtGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGgctggctg < 2:1558908/100‑1 (MQ=255)
tgttgtGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGgctggctg < 2:708933/100‑1 (MQ=255)
gtgATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGc < 2:1089815/100‑1 (MQ=255)
gtgATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGc > 1:1286561/1‑100 (MQ=255)
gATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg > 2:1136994/1‑100 (MQ=255)
gATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg > 2:136185/1‑100 (MQ=255)
gATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg > 1:489632/1‑100 (MQ=255)
gatcttGTTGAAACAGCCGGATTTAACGCCAAAAACGATTCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg < 1:778116/95‑1 (MQ=255)
gatcttGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg < 1:1363150/95‑1 (MQ=255)
aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGGGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg < 1:740696/100‑1 (MQ=255)
aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg > 1:1073156/1‑100 (MQ=255)
aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg < 1:740710/100‑1 (MQ=255)
tCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGa < 1:1441350/100‑1 (MQ=255)
tGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGagatc > 2:1363141/1‑96 (MQ=255)
tGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGagatc > 2:778112/1‑96 (MQ=255)
tGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcacc > 2:508247/1‑100 (MQ=255)
ttGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac < 1:1026931/100‑1 (MQ=255)
ttGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac > 1:576659/1‑100 (MQ=255)
tGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACcaca < 1:136185/100‑1 (MQ=255)
aaaCAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacatt < 1:1172109/100‑1 (MQ=255)
cAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattaca > 1:850663/1‑100 (MQ=255)
aGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattacat > 2:800313/1‑100 (MQ=255)
cGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTc < 1:228447/100‑1 (MQ=255)
cGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTc < 2:1047382/100‑1 (MQ=255)
ggATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTcc < 2:212256/100‑1 (MQ=255)
ggATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGCATGAACAAGGCTGGCTGATGCCGGACACTACATTACAtttac < 1:1061480/100‑3 (MQ=255)
cGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGaa < 2:478145/100‑1 (MQ=255)
cGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACAATTCCACGTCGaa > 2:578122/1‑100 (MQ=255)
ccGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGt > 2:879175/1‑100 (MQ=255)
|
ATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGT > NC_000913/1728347‑1728543
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
