Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,540,239 |
T→A |
L26Q (CTG→CAG) |
feoA → |
ferrous iron transporter, protein A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,540,239 | 0 | T | A | 94.3%
| 174.7
/ 2.3
| 53 | L26Q (CTG→CAG) | feoA | ferrous iron transporter, protein A |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (33/17); total (35/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 |
ATGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCT > NC_000913/3540142‑3540335
|
aTGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGc < 2:1397937/100‑1 (MQ=255)
agaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCttt > 2:820359/1‑100 (MQ=255)
agaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCttt > 2:805615/1‑100 (MQ=255)
gaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTtc > 1:1522606/1‑100 (MQ=255)
gaAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGAGAAATCAGCCCGGCATATCGCCAAAAACAGCTTtc > 1:163413/1‑100 (MQ=255)
aaCAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTTGAAAATCACTGGCTTTTCCCATGAAAACAGCCCGGCATATCGCCAAAAACAGCTTtctc > 1:470647/1‑100 (MQ=255)
aCAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTctct > 2:1461289/1‑100 (MQ=255)
cAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCtt > 1:1472211/1‑100 (MQ=255)
gTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGc > 2:298747/1‑100 (MQ=255)
tAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCa > 1:1208560/1‑100 (MQ=255)
ccTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTAc > 1:65269/1‑100 (MQ=255)
ccTAGGAAATACACTCCAGATACTGCGTGGAAAATCAATGTCTTTTCCCGTGAATTCAGCTCGAAAAAAAAAAAAAAACAGCTTTCTCTTGGCATGTTAc < 2:223348/100‑1 (MQ=255)
tATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCt > 1:411768/1‑100 (MQ=255)
tGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTgg > 1:1037849/1‑100 (MQ=255)
gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGc < 2:1037842/100‑1 (MQ=255)
gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGc > 1:73823/1‑100 (MQ=255)
gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGc < 1:593097/100‑1 (MQ=255)
gCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCAAAAAACAGCTTTCTCTTGGCATGTTACCTGGc < 2:523631/100‑1 (MQ=255)
acTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCtt < 1:1026062/100‑1 (MQ=255)
tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCtttt < 1:361286/100‑1 (MQ=255)
ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa > 2:1250565/1‑100 (MQ=255)
ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa < 1:575038/100‑1 (MQ=255)
ccAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTa > 1:1201010/1‑100 (MQ=255)
gATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAAtg > 2:84730/1‑100 (MQ=255)
tACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAGACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAAtgtg > 2:1006169/1‑100 (MQ=255)
cGTGGAAAATCACTCGCTGTCCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGATATCTCTTGGCATGTTACCTGGCTCCTCTTTTAATctggt < 2:861285/97‑1 (MQ=255)
aaaaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCg < 1:1122743/100‑1 (MQ=255)
aTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTc > 2:1305319/1‑100 (MQ=255)
tCACTTGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTcc < 1:1478905/100‑1 (MQ=255)
aCTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCAc > 2:562396/1‑100 (MQ=255)
ggCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCg > 1:457745/1‑100 (MQ=255)
ccgatctGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAc < 1:267610/94‑1 (MQ=255)
cccGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAccc > 1:1394768/1‑100 (MQ=255)
ccGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAcccc > 1:397616/1‑100 (MQ=255)
cGTGAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCGCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCa > 2:966718/1‑100 (MQ=255)
gAAATCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAcagatcgg > 2:267609/1‑93 (MQ=255)
aaTCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCat < 1:879749/100‑1 (MQ=255)
aTCAGCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCata < 1:1298853/100‑1 (MQ=255)
gCCCGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGa > 1:51783/1‑100 (MQ=255)
cGGCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAc < 1:385722/100‑1 (MQ=255)
gCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAccc > 1:996409/1‑100 (MQ=255)
gCATATCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAccc < 1:558469/100‑1 (MQ=255)
tatCGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCcgtc > 2:779590/1‑100 (MQ=255)
cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg < 1:779594/100‑1 (MQ=255)
cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg > 2:595864/1‑100 (MQ=255)
cGCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtg > 1:423733/1‑100 (MQ=255)
gCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtgt > 2:81187/1‑100 (MQ=255)
gCCAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCgtgt > 1:634473/1‑100 (MQ=255)
ccAAAATCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTGTAATGTGGTGCGCGTCGCTCCACTCGGCGTCCCCATTCATATCGAAACCCGTCGtgtg < 2:98945/100‑1 (MQ=255)
ccAAAAACAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGtgtg > 2:1212383/1‑100 (MQ=255)
aaaaaCTGCTTTCCCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGtgtg > 2:1225166/1‑98 (MQ=255)
aaaaCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGATGCGCGTCGCTCCACTCGGCGACCCCATTCATATCAAAACCCGTCGTGTGa > 2:98766/1‑98 (MQ=255)
aaCAGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCt > 2:1422117/1‑100 (MQ=255)
|
ATGAGAAACAAGTAGGCACCTATGCAATACACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCT > NC_000913/3540142‑3540335
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
