Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236,541 C→T 100% R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5410CT98.9% 286.7 / ‑4.4 88R159* (CGA→TGA) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (55/31);  total (56/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

CACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAG  >  NC_000913/236447‑236637
                                                                                              |                                                                                                
cACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg                                                                                             <  2:1592175/100‑1 (MQ=255)
cACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg                                                                                             >  2:1411810/1‑100 (MQ=255)
 aCCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGc                                                                                            <  1:957191/100‑1 (MQ=255)
 aCCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCACAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg                                                                                             <  2:38130/99‑1 (MQ=255)
   cGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGCACGCTg                                                                                          >  2:878851/1‑100 (MQ=255)
   cGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTg                                                                                          <  1:1542284/100‑1 (MQ=255)
    gATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGc                                                                                         >  2:2407337/1‑100 (MQ=255)
    gATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGc                                                                                         <  1:1766217/100‑1 (MQ=255)
    gATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGc                                                                                         >  1:614005/1‑100 (MQ=255)
        gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg                                                                                     >  2:432290/1‑100 (MQ=255)
          cTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgggg                                                                                   <  1:1529841/100‑1 (MQ=255)
          cTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgggg                                                                                   <  1:2407351/100‑1 (MQ=255)
          cTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGACGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg                                                                                     >  2:1205388/1‑98 (MQ=255)
           ttGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGc                                                                                  >  2:334508/1‑100 (MQ=255)
            tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGc                                                                                  <  2:996597/99‑1 (MQ=255)
            tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa                                                                                 >  2:2425340/1‑100 (MQ=255)
            tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa                                                                                 >  1:463913/1‑100 (MQ=255)
             gCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAt                                                                                >  1:2556918/1‑100 (MQ=255)
              cGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAtt                                                                               <  2:1775138/100‑1 (MQ=255)
                gTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTAc                                                                             >  1:1665312/1‑100 (MQ=255)
                gTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTAc                                                                             >  2:1309871/1‑100 (MQ=255)
                 tGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCTTCGATGCGTTATGTGCTAGCTACGAATTAGATAACAGTAAATGAACGCTGCACGGGGCATTACt                                                                            <  2:1367737/100‑1 (MQ=255)
                  ggCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                           >  2:55962/1‑100 (MQ=255)
                    cGAGGAAAATGTTGCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                         >  1:1742680/1‑100 (MQ=255)
                     gAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGAt                                                                        >  1:611094/1‑100 (MQ=255)
                       ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                      >  1:1349671/1‑100 (MQ=255)
                       ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                      >  1:188797/1‑100 (MQ=255)
                       ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                      >  1:2437772/1‑100 (MQ=255)
                       ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                      >  1:792712/1‑100 (MQ=255)
                       ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                      >  1:392429/1‑100 (MQ=255)
                        gAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGcc                                                                     >  1:358392/1‑100 (MQ=255)
                          aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                   >  2:1071139/1‑100 (MQ=255)
                          aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                   <  1:1335402/100‑1 (MQ=255)
                            aTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGa                                                                 <  2:350417/100‑1 (MQ=255)
                              gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTGGCTAAGAAATACATAACAGTAAATGAAAGCTCCACGGGGCATTACTAGATGCCCAgct                                                                >  2:510110/1‑97 (MQ=255)
                              gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATc                                                               <  1:1991945/100‑1 (MQ=255)
                               tttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATcc                                                              >  1:1950500/1‑100 (MQ=255)
                                ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt                                                             >  2:305834/1‑100 (MQ=255)
                                  cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTAATCGATGCCCAGATCCtt                                                            >  2:2053881/1‑99 (MQ=255)
                                   ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc                                                          <  1:1613659/100‑1 (MQ=255)
                                   ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc                                                          <  2:1380709/100‑1 (MQ=255)
                                         aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                    <  1:1606644/100‑1 (MQ=255)
                                          gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                                   >  1:1659283/1‑100 (MQ=255)
                                          gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                                   >  1:1798148/1‑100 (MQ=255)
                                          gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                                   >  1:2368578/1‑100 (MQ=255)
                                            gcAAGAGTCTCGAGTGGTTATGGGCTCGCTAAGAAATAGATAACAGTAAAGGAACGATGTACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                     <  2:1536517/96‑1 (MQ=255)
                                            gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt                                                  >  2:1916998/1‑99 (MQ=255)
                                             cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTAt                                                >  1:620292/1‑100 (MQ=255)
                                              aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATc                                               >  2:2316122/1‑100 (MQ=255)
                                              aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATc                                               <  2:1426773/100‑1 (MQ=255)
                                              aaCAGCCTAGGTGCGTTATGTGCTCGCTACTAAATAGATACCAGTAGATGAAAGCTGCACGGGGCATTACTCGATGCTCAGATCCTTGCGGAAGTTTATc                                               <  2:1900893/100‑1 (MQ=255)
                                               aCAGCCTCGATGCGTTATGTGCTCGCTACGAAGTAGATAACAGTAAATGAACGCTGCACGGTGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCt                                              >  1:2426694/1‑100 (MQ=255)
                                                  gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc                                           <  2:2332355/100‑1 (MQ=255)
                                                  gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc                                           >  1:1017896/1‑100 (MQ=255)
                                                   ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                          <  1:1037394/100‑1 (MQ=255)
                                                    cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa                                         >  2:2053093/1‑100 (MQ=255)
                                                       gATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGa                                      >  2:1177231/1‑100 (MQ=255)
                                                         tGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc                                    <  2:188795/100‑1 (MQ=255)
                                                         tGCGTCATCTGCTCGCGACGAAATCGAGAAGCGTAAATGAACGCTGCAGTGGGCATTCCTCGATGCCCAGACCCTTGCGAAAGTTTATCTGGCGATGAcc                                    <  2:1421102/100‑1 (MQ=255)
                                                            gTTATGTGCTCGCTACGAAATAGATAACGGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                 >  1:154909/1‑100 (MQ=255)
                                                            gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                 >  1:1286311/1‑100 (MQ=255)
                                                            gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACCGGGAATTACTTGAGGCCCAGATCCTTGCAGAAGTTTATCTGGCGATGACCgg                                  >  2:1796495/1‑99 (MQ=255)
                                                              tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCTGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                               >  1:1802642/1‑100 (MQ=255)
                                                              tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                               <  2:1017888/100‑1 (MQ=255)
                                                              tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                               >  2:605487/1‑100 (MQ=255)
                                                               aTGTGCTCGCTACGACATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt                              >  1:604023/1‑100 (MQ=255)
                                                                tgtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                             >  2:2014105/1‑100 (MQ=255)
                                                                tgtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                             <  2:2302052/100‑1 (MQ=255)
                                                                     tCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg                        <  2:2083609/100‑1 (MQ=255)
                                                                       gCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACATc                      >  2:750352/1‑100 (MQ=255)
                                                                        cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                     >  2:2050564/1‑100 (MQ=255)
                                                                            gAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                 <  1:2010633/100‑1 (MQ=255)
                                                                            gAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                 >  2:254724/1‑100 (MQ=255)
                                                                             aaaTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGc                <  2:1728272/100‑1 (MQ=255)
                                                                               aTAGATAACAGTAAATGAACGCTGCACGGGGCAGTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtt              <  1:1764288/100‑1 (MQ=255)
                                                                                  ggtAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt             <  1:752503/96‑1 (MQ=255)
                                                                                   aTAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc          >  2:498590/1‑100 (MQ=255)
                                                                                      aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt       >  2:2262839/1‑100 (MQ=255)
                                                                                      aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt       >  1:1442226/1‑100 (MQ=255)
                                                                                       cggTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg      <  2:154907/98‑1 (MQ=255)
                                                                                       cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAGGTCGATGGCTTTTGAGATg      >  2:2100139/1‑100 (MQ=255)
                                                                                       cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg      >  2:2327699/1‑100 (MQ=255)
                                                                                       cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg      >  1:2501949/1‑100 (MQ=255)
                                                                                        aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg     <  1:1560769/100‑1 (MQ=255)
                                                                                        aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg     >  2:651313/1‑100 (MQ=255)
                                                                                        aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg     >  1:2282505/1‑100 (MQ=255)
                                                                                         gTAAATGTACGCTGCACGGGGCATTTCTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa    <  2:260004/100‑1 (MQ=255)
                                                                                         gTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa    >  1:1097877/1‑100 (MQ=255)
                                                                                           aaaTGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg  >  2:2238541/1‑100 (MQ=255)
                                                                                           aaaTGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg  >  2:576453/1‑100 (MQ=255)
                                                                                           aaaTGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg  <  1:2219659/100‑1 (MQ=255)
                                                                                              |                                                                                                
CACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAG  >  NC_000913/236447‑236637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A