Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236,541 C→T 100% R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5410CT99.3% 449.6 / ‑4.6 135R159* (CGA→TGA) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (61/72);  total (61/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 142 total aligned reads displayed.

GGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAA  >  NC_000913/236444‑236636
                                                                                                 |                                                                                               
ggTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatga                                                                                               <  2:1033865/100‑1 (MQ=255)
  tCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaacgaac                                                                                             >  2:730472/1‑100 (MQ=255)
   cACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg                                                                                            >  2:3688153/1‑100 (MQ=255)
    aCCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGc                                                                                           >  1:3900720/1‑100 (MQ=255)
    aCCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGc                                                                                           >  1:2393079/1‑100 (MQ=255)
    aCCGAGAGCCTTGCGGTGGCGAGGAAAATGTTTCCTGGTAAGCGCATCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg                                                                                            <  2:1238462/99‑1 (MQ=255)
     ccGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCt                                                                                          >  1:2514148/1‑100 (MQ=255)
     ccGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCt                                                                                          >  2:1482075/1‑100 (MQ=255)
     ccGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGAGTTATGTGCTCGCTAGGAAATAGATAACAGTAAATGAAAGCt                                                                                          >  1:3731715/1‑100 (MQ=255)
      cGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTg                                                                                         <  2:360113/100‑1 (MQ=255)
      cGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTg                                                                                         >  1:2120847/1‑100 (MQ=255)
      cGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTg                                                                                         <  2:143951/100‑1 (MQ=255)
         tAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCAc                                                                                      <  1:1017412/100‑1 (MQ=255)
          aGCCTTGCGGTGGCGAGGAACATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCAc                                                                                      >  2:2556140/1‑99 (MQ=255)
          aGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACg                                                                                     <  1:3218204/100‑1 (MQ=255)
          aGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACg                                                                                     >  1:1245568/1‑100 (MQ=255)
           gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTTCACgg                                                                                    <  2:4054354/100‑1 (MQ=255)
             cTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTTGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgggg                                                                                  <  1:819047/100‑1 (MQ=255)
             cTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgggg                                                                                  >  1:1529444/1‑100 (MQ=255)
               tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa                                                                                <  2:4068515/100‑1 (MQ=255)
               tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa                                                                                >  1:1993651/1‑100 (MQ=255)
                gCGGTGGCGAGGAAAATGTTTCCCGGTAATCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAt                                                                               <  1:1127396/100‑1 (MQ=255)
                gCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAt                                                                               <  2:2997452/100‑1 (MQ=255)
                    tGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACt                                                                           <  1:1296569/100‑1 (MQ=255)
                     ggCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                          >  2:2029993/1‑100 (MQ=255)
                     gccGAGGAAAATGATTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                          >  1:2812259/3‑100 (MQ=255)
                      gCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCg                                                                         >  1:2159167/1‑100 (MQ=255)
                      gCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCg                                                                         <  2:251201/100‑1 (MQ=255)
                      gCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCg                                                                         <  1:4129875/100‑1 (MQ=255)
                       cGAGGAAAATGTTTCCCTGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                        >  2:792737/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACt                                                                           >  2:2000441/1‑97 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                        >  1:1675066/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                        <  1:1509976/100‑1 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                        >  1:498187/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGCGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTTCTCGa                                                                        >  2:2135100/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGAAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                        >  2:1131334/1‑100 (MQ=255)
                         aGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATg                                                                      <  2:3933023/100‑1 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATg                                                                      <  2:681720/99‑1 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                     <  2:881341/100‑1 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                     <  2:1875587/100‑1 (MQ=255)
                           gAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGcc                                                                    >  1:2055354/1‑100 (MQ=255)
                            aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTCTGTGCTCGCTACGAAATCGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGccc                                                                   <  1:1554440/100‑1 (MQ=255)
                             aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                  >  1:741741/1‑100 (MQ=255)
                               aTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGa                                                                <  2:1639577/100‑1 (MQ=255)
                                 gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATc                                                              >  1:3605684/1‑100 (MQ=255)
                                 gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATc                                                              >  2:3748112/1‑100 (MQ=255)
                                   ttaccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt                                                            >  1:352824/4‑100 (MQ=255)
                                    tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt                                                           >  1:1593010/1‑100 (MQ=255)
                                    tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt                                                           >  1:878628/1‑100 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                          <  1:1398117/100‑1 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                          <  1:797587/100‑1 (MQ=255)
                                       cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg                                                        >  1:2642229/1‑100 (MQ=255)
                                       cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg                                                        <  1:3593698/100‑1 (MQ=255)
                                        ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCTTTGCgg                                                       <  1:3160229/100‑1 (MQ=255)
                                        ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg                                                       <  1:3351672/100‑1 (MQ=255)
                                        ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg                                                       <  2:1570781/100‑1 (MQ=255)
                                          tAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCCGATCCTTGCGGaa                                                     <  2:2094181/100‑1 (MQ=255)
                                              cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt                                                 <  1:2101286/100‑1 (MQ=255)
                                              cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt                                                 >  2:1497670/1‑100 (MQ=255)
                                               gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa                                                <  1:16380/100‑1 (MQ=255)
                                                   cAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTg                                            <  1:2343178/100‑1 (MQ=255)
                                                   cAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTg                                            <  2:4120044/100‑1 (MQ=255)
                                                    aGCCTCGATGCGTTATGTGCTCGCTACGAACTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATTCCCAGATCCTTGCGGAAGTTTATCTgg                                           >  2:675557/1‑100 (MQ=255)
                                                      ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                         <  2:2169263/100‑1 (MQ=255)
                                                        tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt                                       >  1:98143/1‑100 (MQ=255)
                                                            tGCGTTATTTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTgg                                           <  2:1107822/92‑1 (MQ=255)
                                                            tGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc                                   <  2:1993641/100‑1 (MQ=255)
                                                             gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCg                                  >  2:888862/1‑100 (MQ=255)
                                                              cGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg                                 >  1:2442853/1‑100 (MQ=255)
                                                               gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                <  2:1118641/100‑1 (MQ=255)
                                                                 tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                              <  2:2169538/100‑1 (MQ=255)
                                                                 tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                              <  1:1670548/100‑1 (MQ=255)
                                                                 tATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTAGATGCCCAGATCCTTGCGGGAGTTTATCTGGCGATGACCggtgg                              >  2:2906855/1‑100 (MQ=255)
                                                                  aTGTGCTCGCTACGAAATAGATCACAGTAAAGGAACGGTGCACGGGGGATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt                             <  2:2812243/100‑1 (MQ=255)
                                                                     tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                          <  2:1203197/100‑1 (MQ=255)
                                                                     tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                          >  2:1343954/1‑100 (MQ=255)
                                                                      gCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa                         >  1:584212/1‑100 (MQ=255)
                                                                      gCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa                         >  1:21027/1‑100 (MQ=255)
                                                                       cTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                        <  1:4062742/100‑1 (MQ=255)
                                                                         cGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                      <  1:679403/100‑1 (MQ=255)
                                                                         cGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGAGGCCAAACGt                      >  2:1155925/1‑100 (MQ=255)
                                                                           cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                    >  2:2956649/1‑100 (MQ=255)
                                                                            tACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGa                   <  2:2748202/100‑1 (MQ=255)
                                                                            tACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGa                   <  1:3878764/100‑1 (MQ=255)
                                                                                  aTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAATCGTCGATGGCtt             <  1:2738824/100‑1 (MQ=255)
                                                                                  aTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtt             <  2:349382/100‑1 (MQ=255)
                                                                                   tAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt            <  2:3605657/100‑1 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg          >  2:363580/1‑100 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg          <  2:39042/100‑1 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg          >  2:2103538/1‑100 (MQ=255)
                                                                                      aTAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc         <  1:4124263/100‑1 (MQ=255)
                                                                                         aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt      <  1:2834956/100‑1 (MQ=255)
                                                                                         aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt      >  2:2760039/1‑100 (MQ=255)
                                                                                         aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACATCGATGGCTTTTGCGa       >  2:3951397/1‑99 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg     >  1:1905382/1‑100 (MQ=255)
                                                                                           aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg    <  2:783589/100‑1 (MQ=255)
                                                                                            gTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTGTATCTGTCGATGACCGGCGGTCAAACGTCGATGGCGATTGCGATgg    >  2:503297/1‑99 (MQ=255)
                                                                                            gTAAATCAACGCTGCACGGTGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa   <  2:3330295/100‑1 (MQ=255)
                                                                                             tAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGaa  >  2:2692054/1‑100 (MQ=255)
                                                                                             tAAATGAACGCTGCACGGGGCATTACGCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa   <  2:1818950/99‑1 (MQ=255)
                                                                                                 |                                                                                               
GGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAA  >  NC_000913/236444‑236636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A