Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,616 C→G 20.5% G145A (GGG→GCG) ‡ yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6160CG20.5% 234.1 / 60.6 107G145A (GGG→GCG) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (46/39);  new base G (10/12);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 111 total aligned reads displayed.

AGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACC  >  NC_000913/2449517‑2449714
                                                                                                    |                                                                                                  
aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGcc                                                                                                    <  1:67606/100‑1 (MQ=255)
    tGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTg                                                                                                >  1:2070919/1‑100 (MQ=255)
      aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGACCCCTGAt                                                                                              >  2:366911/1‑100 (MQ=255)
         aGTTTTTACTTACTGACATAATTCGCATTCCATGAGACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACg                                                                                           <  1:1472487/100‑1 (MQ=255)
         aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACg                                                                                           <  2:1462340/100‑1 (MQ=255)
           ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtc                                                                                          <  1:2198840/100‑4 (MQ=38)
           ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTc                                                                                         <  2:579604/100‑1 (MQ=255)
           ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTc                                                                                         <  1:1614300/100‑1 (MQ=255)
            tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtca                                                                                         <  1:900328/100‑5 (MQ=38)
              tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACg                                                                                      <  1:849901/100‑1 (MQ=255)
                aCTTACTGACATAATTCTCATTCCATGAAACGTCATACGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGcc                                                                                    >  1:270163/1‑100 (MQ=255)
                aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgac                                                                                    <  1:2320901/100‑3 (MQ=37)
                 cTTACTGACATAATTGTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgaca                                                                                   <  1:1582688/100‑4 (MQ=25)
                 cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgaca                                                                                   <  2:381046/100‑4 (MQ=37)
                  ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgacac                                                                                  <  1:1511023/100‑5 (MQ=25)
                  ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgacac                                                                                  <  2:444922/100‑5 (MQ=37)
                  ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgacac                                                                                  <  1:1184045/100‑5 (MQ=25)
                   tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGccccc                                                                                 <  2:2522114/100‑1 (MQ=255)
                   tACTGACAAAATTCTCATTCCATGAAACGTCATAAGGTTGAAAATAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgacac                                                                                  <  2:556537/99‑5 (MQ=21)
                    aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCt                                                                                >  1:2229503/1‑100 (MQ=255)
                     cTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTg                                                                               <  1:935663/100‑1 (MQ=255)
                       gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTCAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                             <  2:2552084/100‑1 (MQ=255)
                        aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATA‑GTCACgacacgatttg                                                                            <  2:1940078/100‑11 (MQ=21)
                         cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                           >  1:2114237/1‑100 (MQ=255)
                         cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACCGACGGACAAATCGTGTCGTGAG‑TATCAGGCCCCTGATAAGTCACGCCCCCTGgag                                                                            >  2:1623240/1‑99 (MQ=255)
                          aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                          >  1:771113/1‑100 (MQ=255)
                          aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                          <  1:988917/100‑1 (MQ=255)
                           tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAgg                                                                         <  1:2338705/100‑1 (MQ=255)
                              ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                      >  1:1132391/1‑100 (MQ=255)
                              ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                      >  1:1165312/1‑100 (MQ=255)
                                ctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                    <  2:1701709/100‑1 (MQ=255)
                                 tcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                   <  1:2310292/100‑1 (MQ=255)
                                   aTTCCATGAAACGTCATAAGGTTGAACAGAAACTTATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 <  2:627969/100‑1 (MQ=255)
                                   aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 >  2:225790/1‑100 (MQ=255)
                                   aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 <  1:895163/100‑1 (MQ=255)
                                   aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                   >  1:640378/1‑98 (MQ=255)
                                    ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                >  1:1314239/1‑100 (MQ=255)
                                    ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATATGGCGCCTGATACGTCACGCCCCCTGAAGAGCCATCaga                                                                  >  2:801806/1‑98 (MQ=255)
                                     tCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGCTACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                >  2:703993/1‑99 (MQ=255)
                                      ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:2250036/1‑100 (MQ=255)
                                      ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:1538678/1‑100 (MQ=255)
                                      ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:13988/1‑100 (MQ=255)
                                       cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                             >  1:1738622/1‑100 (MQ=255)
                                       cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                             >  2:1687271/1‑100 (MQ=255)
                                        aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                            <  1:249851/100‑1 (MQ=255)
                                         tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattc                                                           <  2:610079/100‑1 (MQ=255)
                                           aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                         <  1:1228467/100‑1 (MQ=255)
                                             aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                       <  1:225792/100‑1 (MQ=255)
                                                tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAG‑TATAAGGCCCCTGATCAGTAACGCCACCTGGAGAGGCTTCACAGTCATCGAtaataa                                                    >  2:758236/1‑100 (MQ=255)
                                                  aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                  <  2:446188/100‑1 (MQ=255)
                                                  aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                  <  2:447405/100‑1 (MQ=255)
                                                      ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                              >  1:208585/1‑100 (MQ=255)
                                                        ttGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                            <  2:2090136/100‑1 (MQ=255)
                                                            aCAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                        >  2:1303574/1‑100 (MQ=255)
                                                               gAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                     >  2:1066272/1‑100 (MQ=255)
                                                               gAAACGGATGGACAAATCGGGTCGTTAC‑TATCAGGCCCCTGATACGGCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                     >  2:2040063/1‑100 (MQ=255)
                                                                    ggATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                <  2:2092835/100‑1 (MQ=255)
                                                                     gATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGt                               <  2:2001881/100‑1 (MQ=255)
                                                                      aTGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                              <  1:580623/100‑1 (MQ=255)
                                                                       tGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTaa                             >  1:2077457/1‑100 (MQ=255)
                                                                        ggACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGACACGCCCCCTGGAGAGGCTCCAGAGTCATTCAAAATAACCAACCATGGACAAGAAAAGTaa                             >  2:1115991/1‑99 (MQ=255)
                                                                         gACAAATCGTGTCGTGAT‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                           >  1:1572623/1‑100 (MQ=255)
                                                                          aCAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                          >  1:982301/1‑100 (MQ=255)
                                                                          aCAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                          <  2:1098496/100‑1 (MQ=255)
                                                                           cAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                         >  2:1898912/1‑100 (MQ=255)
                                                                            aaaTCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGTCCAAGAAAAGTAAg                            >  1:443008/1‑96 (MQ=255)
                                                                             aaTCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                       >  1:2036447/1‑100 (MQ=255)
                                                                             aaTCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                       >  1:2036819/1‑100 (MQ=255)
                                                                               tcgtgtcgtgAC‑TATCAGGCCCCTGATGCGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAATCCAt                     >  2:113805/1‑100 (MQ=255)
                                                                                gggggcgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                    >  2:450929/6‑100 (MQ=25)
                                                                                   gtcgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGACAAGTAAGATTCAATctct                 >  2:2209606/1‑100 (MQ=255)
                                                                                   gtcgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGagccgaatca                  >  2:360058/1‑90 (MQ=255)
                                                                                   gtcgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                 >  1:697953/1‑100 (MQ=255)
                                                                                   ggcgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                 >  1:1403256/3‑100 (MQ=37)
                                                                                    tcgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                <  1:2020752/100‑1 (MQ=255)
                                                                                     cgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:683753/1‑100 (MQ=38)
                                                                                     cgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  2:2202790/1‑100 (MQ=38)
                                                                                     cgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:429311/1‑100 (MQ=38)
                                                                                     cgtgACGTATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:1413950/1‑100 (MQ=38)
                                                                                     cgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  2:867610/1‑100 (MQ=255)
                                                                                     cgtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  2:1090042/1‑100 (MQ=255)
                                                                                      gtgacgtATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              <  2:630509/94‑1 (MQ=38)
                                                                                      gtgacgtATCAGGGGCCTGATA‑GTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  1:156223/7‑100 (MQ=38)
                                                                                      gtgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  2:1235169/1‑100 (MQ=255)
                                                                                       tgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:486285/100‑1 (MQ=255)
                                                                                       tgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:1097809/100‑1 (MQ=255)
                                                                                       tgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:310254/100‑1 (MQ=255)
                                                                                       tgAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  2:464052/1‑99 (MQ=255)
                                                                                        gAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga            >  1:1697477/1‑100 (MQ=255)
                                                                                        gAC‑TATCAGGCCCCTGATACCTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga            <  2:2467262/100‑1 (MQ=255)
                                                                                         aC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag           <  1:606492/100‑1 (MQ=255)
                                                                                         aC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag           <  2:1165304/100‑1 (MQ=255)
                                                                                            tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc         <  2:460642/100‑1 (MQ=255)
                                                                                            tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc         <  1:1434851/100‑1 (MQ=255)
                                                                                              tCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACaa       >  1:1588234/1‑100 (MQ=255)
                                                                                                aGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATa     <  2:443004/100‑1 (MQ=255)
                                                                                                 ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATaa    >  1:438133/1‑100 (MQ=255)
                                                                                                   ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  >  1:293986/1‑100 (MQ=255)
                                                                                                   ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  <  1:1804935/100‑1 (MQ=255)
                                                                                                   ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  <  1:2412908/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
AGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAC‑TATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACC  >  NC_000913/2449517‑2449714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A