Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I1 R1 174 80.0 3892211 96.7% 3763768 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,756 G→A intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7560GA94.1% 261.7 / 5.2 86intergenic (+77/‑14)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (35/45);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810660‑2810852
                                                                                                |                                                                                                
aaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAg                                                                                                <  1:483211/99‑1 (MQ=255)
 aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                              <  2:1447904/100‑1 (MQ=255)
  gTGATGGCTATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  1:1927127/100‑1 (MQ=255)
  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             >  1:260399/1‑100 (MQ=255)
  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  1:1762541/100‑1 (MQ=255)
  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  2:173482/100‑1 (MQ=255)
  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  1:532322/100‑1 (MQ=255)
    gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCAt                                                                                           >  1:1011970/1‑100 (MQ=255)
     aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                          >  2:1499410/1‑100 (MQ=255)
      tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                         <  1:1751631/100‑1 (MQ=255)
       ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAcc                                                                                        <  2:139761/100‑1 (MQ=255)
       ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAcc                                                                                        >  2:69710/1‑100 (MQ=255)
        gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAccc                                                                                       <  2:1439798/100‑1 (MQ=255)
         cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                      >  1:1035872/1‑100 (MQ=255)
             tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                  <  1:493457/100‑1 (MQ=255)
              atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATgg                                                                                 >  2:1274115/1‑100 (MQ=255)
               tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGa                                                                                <  1:240160/100‑1 (MQ=255)
                ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                               >  1:1484555/1‑100 (MQ=255)
                 taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATa                                                                              >  1:709388/1‑100 (MQ=255)
                  aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             >  2:695089/1‑100 (MQ=255)
                  aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             <  1:1093961/100‑1 (MQ=255)
                   aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGt                                                                            <  1:143709/100‑1 (MQ=255)
                   aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGt                                                                            <  2:1600757/100‑1 (MQ=255)
                     ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGtt                                                                           >  1:1272518/1‑99 (MQ=255)
                      cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         <  2:1444019/100‑1 (MQ=255)
                       acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         >  2:139858/1‑99 (MQ=255)
                        caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGt                                                                        >  2:1926403/1‑99 (MQ=255)
                         aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      <  1:618427/100‑1 (MQ=255)
                          aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTa                                                                     >  1:758750/1‑100 (MQ=255)
                          aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTa                                                                     >  2:7796/1‑100 (MQ=255)
                           gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                    <  2:995832/100‑1 (MQ=255)
                            aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                   >  1:1285625/1‑100 (MQ=255)
                             aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                    >  1:595190/1‑98 (MQ=255)
                                aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               >  2:1087203/1‑100 (MQ=255)
                                   tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                            <  2:1475203/100‑1 (MQ=255)
                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGaan                                                         <  1:1516605/100‑2 (MQ=255)
                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         >  1:114975/1‑100 (MQ=255)
                                       tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        >  2:82471/1‑100 (MQ=255)
                                       tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGCTCATACGCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        >  1:1365860/1‑100 (MQ=255)
                                       tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGATCATACACAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        >  1:1366048/1‑100 (MQ=255)
                                        aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                       >  1:627355/1‑100 (MQ=255)
                                        aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                       >  1:880013/1‑100 (MQ=255)
                                         aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                      <  2:401131/100‑1 (MQ=255)
                                         aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCACATTGAACaaa                                                      >  1:566035/1‑100 (MQ=255)
                                          aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                     <  1:1263780/100‑1 (MQ=255)
                                            cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                   <  1:1556335/100‑1 (MQ=255)
                                                aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATCAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                               <  1:408729/100‑1 (MQ=255)
                                                  aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACATATGCTAAAAt                                             <  1:1368954/100‑1 (MQ=255)
                                                  aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                             <  2:665350/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         <  1:1777110/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         <  1:1198185/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         >  2:1466496/1‑100 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         <  2:1365868/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         <  2:1285632/100‑1 (MQ=255)
                                                       cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                        >  2:1566435/1‑100 (MQ=255)
                                                        tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                       <  2:758751/100‑1 (MQ=255)
                                                         ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                      >  1:745263/1‑100 (MQ=255)
                                                         ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                      <  2:485384/100‑1 (MQ=255)
                                                         ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                      <  1:1659558/100‑1 (MQ=255)
                                                            tttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                   <  1:1473330/100‑1 (MQ=255)
                                                             ttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                  <  2:231598/100‑1 (MQ=255)
                                                             ttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                  <  1:1760703/100‑1 (MQ=255)
                                                              tGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                 >  1:496714/1‑100 (MQ=255)
                                                               gTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                <  1:1550236/100‑1 (MQ=255)
                                                                    tGTCGCTACTATATCGGCTGTACTTAAGCAGGTCATACGCACATGGATAGTTCGCTTACGCCCATTGAACAAATGTTAAAATTTCGCGcc                                     <  1:379568/90‑1 (MQ=255)
                                                                       cGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        >  2:1263875/1‑100 (MQ=255)
                                                                       cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        >  1:430724/1‑100 (MQ=255)
                                                                        gTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                       <  1:1913966/100‑1 (MQ=255)
                                                                         ttACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                      >  2:1080280/1‑100 (MQ=255)
                                                                          tACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                     <  2:579575/100‑1 (MQ=255)
                                                                          tACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                     >  1:1379156/1‑100 (MQ=255)
                                                                            cTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                   >  2:737980/1‑100 (MQ=255)
                                                                             tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                  <  2:1719832/100‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:481216/100‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:1289632/100‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:752837/100‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  1:82471/100‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  1:573723/100‑1 (MQ=255)
                                                                                  cGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAACTTCCGCGCCAGCCGCCACGAAGATTTTCCTTAt             <  1:1281049/100‑1 (MQ=255)
                                                                                   ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTagc            >  1:1183717/1‑98 (MQ=255)
                                                                                     cTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg          >  2:1549531/1‑100 (MQ=255)
                                                                                      tGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg         >  2:1174781/1‑100 (MQ=255)
                                                                                      tGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg         >  2:964725/1‑100 (MQ=255)
                                                                                      tGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg         <  2:496716/100‑1 (MQ=255)
                                                                                        aaaTTAATAATGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGTGCCAGCCGCCACGAAGATTTTCCTTATCAGgag       <  1:1274110/100‑1 (MQ=255)
                                                                                          aataataaGGTCAGACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt     >  2:1319406/3‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc   >  1:114202/1‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc   >  2:784645/1‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATc    >  1:1660867/1‑99 (MQ=255)
                                                                                             aaTAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt  >  1:912351/1‑100 (MQ=255)
                                                                                                |                                                                                                
AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810660‑2810852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A