Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,756 G→A 100% intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7560GA99.2% 385.9 / ‑4.7 118intergenic (+77/‑14)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (56/61);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 119 total aligned reads displayed.

AAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810659‑2810852
                                                                                                 |                                                                                                
aaaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAg                                                                                                <  2:2183751/100‑1 (MQ=255)
 aaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAgg                                                                                               <  2:2097386/100‑1 (MQ=255)
   gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                              >  1:2758010/1‑99 (MQ=255)
   gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  2:1493541/100‑1 (MQ=255)
   gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                             <  2:1878017/100‑1 (MQ=255)
   gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATAGGCTGAAATTAATAAGGTc                                                                                             >  1:1101561/1‑100 (MQ=255)
    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCa                                                                                            >  2:2724374/1‑100 (MQ=255)
     gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCAt                                                                                           >  2:1443574/1‑100 (MQ=255)
       tttctaTTATATAACCCAATAGAATCATTTTTCTAAAACGATACATTTACGTTATTTGACACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                         <  1:2938956/95‑1 (MQ=255)
       tGGCGATTATATACCCCACAAGAAAAATTTTTCTAAAACAATACATTAACTTTATTTGTAACTGTCATTACTATCTCGTCTGAACTTAATAAGCTc                                                                                             >  2:3035497/1‑96 (MQ=255)
         gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                          >  2:201076/1‑97 (MQ=255)
          cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                      <  2:1535887/100‑1 (MQ=255)
           gATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCaa                                                                                     >  2:2067557/1‑100 (MQ=255)
             ttATATAACCCACAAGAATCATTTTTCTAAAACCATACATGTACTTTATTTGTCACTGTCGTTACTATATCGGCTGTAATTAATAAGGTCATACCCAAAt                                                                                   <  2:1448286/100‑1 (MQ=255)
              tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                  <  1:1318614/100‑1 (MQ=255)
               atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTTTATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                  >  1:750408/1‑99 (MQ=255)
                 ataACCCACAAGAATCTTTTTTCTATAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                               <  1:896054/100‑1 (MQ=255)
                 ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCTTACCCAAATggtt                                                                               <  1:2056193/100‑3 (MQ=255)
                 ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                               >  2:451583/1‑100 (MQ=255)
                 ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                               >  2:2828004/1‑100 (MQ=255)
                  taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATa                                                                              >  1:1231640/1‑100 (MQ=255)
                  taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATa                                                                              <  2:182344/100‑1 (MQ=255)
                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCTTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             <  2:2644638/100‑1 (MQ=255)
                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             <  2:440195/100‑1 (MQ=255)
                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGACTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             >  2:1573319/1‑100 (MQ=255)
                    aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGt                                                                            <  2:856179/100‑1 (MQ=255)
                     cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCCGAAATTAATAAGGTCATACCCAAATGGATAGtt                                                                           <  1:2196513/100‑1 (MQ=255)
                       cacaATAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         <  2:1873174/100‑1 (MQ=255)
                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         >  1:2852290/1‑100 (MQ=255)
                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGtt                                                                       <  1:300734/100‑1 (MQ=255)
                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGtt                                                                       <  1:2976381/100‑1 (MQ=255)
                          aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      <  2:1450844/100‑1 (MQ=255)
                          aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      <  2:1033222/100‑1 (MQ=255)
                            gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                    >  2:383606/1‑100 (MQ=255)
                             aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                   <  2:1688831/100‑1 (MQ=255)
                             aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                   <  1:2251780/100‑1 (MQ=255)
                               tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGGAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGcc                                                                 <  2:1635866/100‑1 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGCAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               >  2:2985912/1‑100 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               <  1:2879163/100‑1 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               >  1:178713/1‑100 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               <  1:1145820/100‑1 (MQ=255)
                                  tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                              <  2:1669396/100‑1 (MQ=255)
                                   ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                             <  1:2982454/100‑1 (MQ=255)
                                    tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAttn                                                            <  2:2481880/100‑2 (MQ=255)
                                     ttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                           <  2:2304023/100‑1 (MQ=255)
                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         >  2:2699951/1‑100 (MQ=255)
                                           aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                     <  2:1795517/100‑1 (MQ=255)
                                            aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                    >  2:1368481/1‑100 (MQ=255)
                                             cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                   <  2:1521781/100‑1 (MQ=255)
                                             cAATACATTTACTTTATTTGTCACTGTCGATACTATATCGGCTGAAATTAATAAGGTCATACCAAAATGGATAATTCGTTTACTCCCATTGAACAAATg                                                    >  2:2730923/1‑99 (MQ=255)
                                              aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                  >  2:2083800/1‑100 (MQ=255)
                                              aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                  <  2:861343/100‑1 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                              >  2:2260010/1‑100 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                              <  1:2262048/100‑1 (MQ=255)
                                                   aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                             <  1:1612104/100‑1 (MQ=255)
                                                    tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                            <  2:2795569/100‑1 (MQ=255)
                                                    tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                            >  1:1138371/1‑100 (MQ=255)
                                                      tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                          >  2:1554910/1‑100 (MQ=255)
                                                      tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                          >  1:623877/1‑100 (MQ=255)
                                                      tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGATCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                          >  1:373419/1‑100 (MQ=255)
                                                       aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                         <  1:1398791/100‑1 (MQ=255)
                                                       aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAAGCCAATTGAACAAAAGATAAAAt                                             >  1:1494911/1‑96 (MQ=255)
                                                        cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                        >  1:1279509/1‑100 (MQ=255)
                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                       <  2:2980516/100‑1 (MQ=255)
                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                       <  2:2980517/100‑1 (MQ=255)
                                                          ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACACCCATTGAACAAATGCTAAAATTTcg                                         >  1:1152647/1‑97 (MQ=255)
                                                           tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                     <  2:1061875/100‑1 (MQ=255)
                                                           tATTTGTCACTGTCGTAAATAAATCGGCTCAAATTAATAAGGTCAAACAAAAATGAATAGTGAGTTTACGCCCATTGAAAAAATGCTAAAATTTCgcgc                                      >  2:1381837/1‑99 (MQ=255)
                                                            aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAATGTCATACCCAAATGGATAGTTCTTGTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                     <  2:1944012/99‑1 (MQ=255)
                                                               tGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                 >  1:1010440/1‑100 (MQ=255)
                                                               tGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                 >  1:1514284/1‑100 (MQ=255)
                                                                gTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                <  1:1212252/100‑1 (MQ=255)
                                                                gTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                 >  2:2251472/1‑99 (MQ=255)
                                                                 tCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                               <  1:1554928/100‑1 (MQ=255)
                                                                  cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcgagatcggaa                              >  2:1179676/1‑91 (MQ=255)
                                                                    cTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                             >  2:480185/1‑99 (MQ=255)
                                                                     tGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                           <  1:1954381/100‑1 (MQ=255)
                                                                      gTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTAAACAAATACTAAAATTTCGCGCCAGCCGCCACGa                          >  1:1195877/1‑100 (MQ=255)
                                                                        cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCTCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        <  2:1658478/100‑1 (MQ=255)
                                                                          ttACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                      <  1:2870347/100‑1 (MQ=255)
                                                                          ttACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                      <  2:2691087/100‑1 (MQ=255)
                                                                            aCTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                    >  2:1482927/1‑100 (MQ=255)
                                                                              tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                   <  1:1464612/99‑1 (MQ=255)
                                                                              tataTCGGCTGAAATTAATAAGGACATACCCAAATGGATAGTTCGTTTAAGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                  >  2:2752064/1‑100 (MQ=255)
                                                                               atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:841392/100‑1 (MQ=255)
                                                                               atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:68875/100‑1 (MQ=255)
                                                                                tatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                >  1:2327320/1‑100 (MQ=255)
                                                                                tatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                >  1:2427361/1‑100 (MQ=255)
                                                                                 atCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATATCGCGCCAGCCGCCACGAAGATTTTCCtt               >  1:471054/1‑100 (MQ=255)
                                                                                    ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTTGTTTACGCCCATTGAACAAATGCGAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc            <  1:1664537/100‑1 (MQ=255)
                                                                                    ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGCACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt             >  2:1092981/1‑99 (MQ=255)
                                                                                    ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc            <  2:731894/100‑1 (MQ=255)
                                                                                        gAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga        >  1:24419/1‑100 (MQ=255)
                                                                                        gAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga        >  1:1099087/1‑100 (MQ=255)
                                                                                         aaaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag       <  2:1010434/100‑1 (MQ=255)
                                                                                          aaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga      >  1:726008/1‑100 (MQ=255)
                                                                                           aTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt     >  1:2740504/1‑100 (MQ=255)
                                                                                           aTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACACCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt     >  2:1615180/1‑100 (MQ=255)
                                                                                             taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc   <  1:929618/100‑1 (MQ=255)
                                                                                              aaTAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt  >  2:2344701/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810659‑2810852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A