Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I1 R1
|
174 |
80.0 |
3892211 |
96.7% |
3763768 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,541,962 |
T→G |
I519S (ATC→AGC) |
feoB → |
ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,541,962 | 0 | T | G | 90.7%
| 249.9
/ 14.6
| 75 | I519S (ATC→AGC) | feoB | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (35/33); total (38/37) |
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 |
TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC > NC_000913/3541866‑3542056
|
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcat < 2:1530528/100‑1 (MQ=255)
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcat > 1:1734311/1‑100 (MQ=255)
gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcatcatc > 1:866171/1‑100 (MQ=255)
ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCg < 1:831354/100‑1 (MQ=255)
ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCg < 1:478042/100‑1 (MQ=255)
tGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGt > 2:762670/1‑100 (MQ=255)
gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc < 1:1007274/100‑1 (MQ=255)
gCCGGTCGATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc > 2:1023079/1‑100 (MQ=255)
ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCa < 2:876108/100‑1 (MQ=255)
cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg < 1:1772985/100‑1 (MQ=255)
cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg < 1:1705726/100‑1 (MQ=255)
cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTCCAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg > 1:14282/1‑100 (MQ=255)
tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCAt < 1:636439/100‑1 (MQ=255)
cTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtt > 2:437714/1‑100 (MQ=255)
ntCATGTACCACACGTTAAAAGACTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTCCGTGCTGGTAAAGTGGGCATCATCGTCAGCAtttt > 1:909783/2‑100 (MQ=255)
aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt > 2:524339/1‑100 (MQ=255)
aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt < 2:845818/100‑1 (MQ=255)
aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt > 2:729321/1‑100 (MQ=255)
aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCt > 2:1192601/1‑100 (MQ=255)
tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTg < 1:119972/100‑1 (MQ=255)
tACCAGACGTTAAAGGCATCTTTATCTTGACTTGGCAGCGTCTGAAAGGCTTCGTTCTGTGTGCTGGTAAAGTGAGCATCATCTTCCGCATTTGCCTgn < 1:1159449/99‑2 (MQ=255)
aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgc > 1:267031/1‑100 (MQ=255)
ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcg > 1:12679/1‑100 (MQ=255)
cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcgc > 2:865770/1‑100 (MQ=255)
acacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCt > 1:790226/1‑100 (MQ=255)
acacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCt < 1:1777496/100‑1 (MQ=255)
cacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCtt > 1:1284797/1‑100 (MQ=255)
cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc > 2:243881/1‑100 (MQ=255)
cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:92867/100‑1 (MQ=255)
cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:14247/100‑1 (MQ=255)
cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:1580062/100‑1 (MQ=255)
gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCa < 2:1137915/100‑1 (MQ=255)
gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCa < 1:1811767/100‑1 (MQ=255)
ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCaa > 2:1252735/1‑100 (MQ=255)
ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCaa > 1:1706903/1‑100 (MQ=255)
tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAAc < 2:1619851/100‑1 (MQ=255)
aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg > 1:339608/1‑100 (MQ=255)
aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg < 1:1221274/100‑1 (MQ=255)
aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGc < 1:60446/100‑1 (MQ=255)
gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCtt > 1:1385995/1‑100 (MQ=255)
tGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTtctc < 1:566512/100‑1 (MQ=255)
gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCg > 2:1615492/1‑100 (MQ=255)
aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGc < 1:602453/100‑1 (MQ=255)
ttATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCt > 2:1004088/1‑100 (MQ=255)
aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGa > 2:1048500/1‑100 (MQ=255)
cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg < 1:1458299/100‑1 (MQ=255)
aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCgg < 2:163405/100‑1 (MQ=255)
gACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCggg < 1:864789/100‑1 (MQ=255)
ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaa > 2:1839265/1‑100 (MQ=255)
ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaa > 2:425783/1‑100 (MQ=255)
gCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt < 1:1846701/99‑1 (MQ=255)
cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg > 1:1172729/1‑100 (MQ=255)
cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg < 2:35058/100‑1 (MQ=255)
aGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgt > 2:437386/1‑100 (MQ=255)
gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc < 1:1107654/100‑1 (MQ=255)
cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtcg < 2:866172/100‑1 (MQ=255)
cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtcg < 1:1176217/100‑1 (MQ=255)
gTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGa > 2:1302878/1‑100 (MQ=255)
tCTGAAAGGCTTCGTTCTGCGTGCTCGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt < 2:530400/100‑1 (MQ=255)
cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATa > 2:1801779/1‑100 (MQ=255)
tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa < 1:1252731/100‑1 (MQ=255)
tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa > 1:1447672/1‑100 (MQ=255)
gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAc > 1:1524001/1‑100 (MQ=255)
aGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATc < 2:209751/100‑1 (MQ=255)
tGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgc < 2:1677492/100‑1 (MQ=255)
gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 1:961818/1‑100 (MQ=255)
gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 2:1684386/1‑100 (MQ=255)
gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 1:961811/1‑100 (MQ=255)
tGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcgc > 1:325610/1‑98 (MQ=255)
gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:1801768/100‑1 (MQ=255)
gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:76083/100‑1 (MQ=255)
gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:762669/100‑1 (MQ=255)
ggTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt > 2:1939626/1‑100 (MQ=255)
ggTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt > 2:1939478/1‑100 (MQ=255)
aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:1619003/1‑100 (MQ=255)
aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:507750/1‑100 (MQ=255)
aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg < 2:1447680/100‑1 (MQ=255)
aGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgtc > 2:861127/1‑100 (MQ=255)
|
TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC > NC_000913/3541866‑3542056
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
