Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I1 R1
|
174 |
80.0 |
3892211 |
96.7% |
3763768 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,950,468 |
G→T |
intergenic (+48/‑39) |
ilvL → / → ilvX |
ilvG operon leader peptide/uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,950,468 | 0 | G | T | 92.5%
| 199.4
/ 9.9
| 67 | intergenic (+48/‑39) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (23/39); total (24/43) |
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 |
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA > NC_000913/3950371‑3950564
|
tgATTATGATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGttt > 2:1024781/1‑100 (MQ=255)
ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttt > 2:1298992/1‑100 (MQ=255)
ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttttt > 2:404118/1‑100 (MQ=255)
ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttttt < 1:884376/100‑1 (MQ=255)
atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttttttg < 2:1778198/100‑3 (MQ=255)
cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc < 2:709072/100‑1 (MQ=255)
cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc < 1:1732804/100‑1 (MQ=255)
cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc < 2:1810465/100‑1 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc < 2:1745947/98‑1 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt > 2:1286238/1‑100 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt > 2:1075235/1‑100 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCtt < 1:1691319/100‑1 (MQ=255)
cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCtt < 1:373820/100‑1 (MQ=255)
aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTa > 1:1339593/1‑100 (MQ=255)
aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCGTAACGAACTAAGACCCCCGCGCCGAAAGGTCCGGGGTTTTTTTTTGACCTTa < 1:525313/100‑1 (MQ=255)
ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaa < 2:1028235/100‑1 (MQ=255)
cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaa < 1:1573337/100‑1 (MQ=255)
gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaa > 2:1838861/1‑100 (MQ=255)
tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa < 2:1117427/100‑1 (MQ=255)
tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa < 2:1528496/100‑1 (MQ=255)
gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAAc < 1:449417/100‑1 (MQ=255)
cGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa < 1:234309/98‑1 (MQ=255)
cGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACa < 1:973286/100‑1 (MQ=255)
ggggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaacata < 2:1243733/100‑5 (MQ=255)
ggtGCACTTGGACGAGGAAGGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc < 2:21582/98‑1 (MQ=255)
gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc > 2:1687472/1‑100 (MQ=255)
gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACATTAAAAACATAAc < 1:582489/100‑1 (MQ=255)
cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAcc < 1:1030263/100‑1 (MQ=255)
gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCg > 1:814080/1‑99 (MQ=255)
ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa > 1:1067149/1‑100 (MQ=255)
cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc < 1:784537/100‑1 (MQ=255)
gAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa < 1:842574/100‑1 (MQ=255)
aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa < 2:80720/100‑1 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc > 2:953352/1‑100 (MQ=255)
agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc < 2:432997/100‑1 (MQ=255)
gagaTCAAGCCTTAACGACCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca < 1:1075234/100‑1 (MQ=255)
gagaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca < 1:1286231/100‑1 (MQ=255)
gagaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca > 2:457923/1‑100 (MQ=255)
gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca > 1:1448213/1‑100 (MQ=255)
cAAGCCTTAACGAACTAAGACCCCCGCACCAAAGGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATACCAGCACaaaa > 1:1278156/1‑100 (MQ=255)
aGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc < 2:729028/100‑1 (MQ=255)
gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc > 1:1142509/1‑100 (MQ=255)
ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt > 1:820408/1‑100 (MQ=255)
cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg < 1:193326/100‑1 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt < 1:596364/100‑1 (MQ=255)
aaCGAACTAAGACCCCCGCACCGAAAGGGCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCATACAATGAATAACAGCACAAAATTCTtttt > 1:673528/1‑100 (MQ=255)
aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGCGCAGACAATGAATAACAGCACAAAATTCTGTTtc > 2:1834592/1‑100 (MQ=255)
aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 2:688949/100‑1 (MQ=255)
cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTTTTTTTTAAACAAAACCGAGGAGCAGACAATGAATAACAGCACGAATTTCTGTTtc > 1:98828/1‑99 (MQ=255)
aaCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa < 1:1244831/100‑1 (MQ=255)
aCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa < 2:1567292/100‑1 (MQ=255)
tAAGCCCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa < 2:1339601/100‑1 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTTTTTTTAAAACATAAACGAAGAGCAGACAATGAATAAGAGCACAGAATTCCTTTTCTCaa > 1:813877/1‑98 (MQ=255)
aaGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAt > 1:802712/1‑100 (MQ=255)
aGACCCCCGCACCGAAATGGCCGGGGTTTTTTTTTGACATTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa < 2:961515/95‑1 (MQ=255)
gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTCTCTTAAGATTc < 1:1024780/100‑1 (MQ=255)
gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc < 2:1517181/100‑1 (MQ=255)
aCCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa < 1:1687462/100‑1 (MQ=255)
cccccGCAGCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc < 1:1277695/98‑1 (MQ=255)
cccccGCACCGAAAGGTCC‑GGGGTTGTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg < 1:1149626/99‑1 (MQ=255)
cacGCATCGAAAGGTCCGGGGTTTTTTTTTGACATTAAAAACATAACCGAGGAGCAGACAATGACTAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa < 2:1334853/98‑1 (MQ=255)
ccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa < 2:1383250/99‑1 (MQ=255)
cACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg < 1:1349853/100‑1 (MQ=255)
ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa > 2:763715/1‑100 (MQ=255)
cGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaa > 2:262423/1‑100 (MQ=255)
aGGTCCGGGGTTTTTTTTTTTTTTTTTAAACATAACCGAGGAGCAGACAATGAATTACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaa > 1:591779/1‑100 (MQ=255)
ggTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaac > 2:1618932/1‑100 (MQ=255)
gTCCGGGGTTTTTTTTTTTCTTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaa > 1:1190672/1‑98 (MQ=255)
tCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta < 2:969574/100‑1 (MQ=255)
gggtTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGaa > 2:605411/1‑100 (MQ=255)
|
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA > NC_000913/3950371‑3950564
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
