Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,950,468 |
G→T |
intergenic (+48/‑39) |
ilvL → / → ilvX |
ilvG operon leader peptide/uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,950,468 | 0 | G | T | 95.7%
| 223.4
/ 1.9
| 70 | intergenic (+48/‑39) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (30/37); total (31/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 |
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG > NC_000913/3950371‑3950562
|
tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGATTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑ttt > 2:93111/1‑100 (MQ=255)
gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttt < 2:683786/100‑1 (MQ=255)
ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttttt < 2:906127/100‑1 (MQ=255)
tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑Ttttttt > 1:1606682/1‑100 (MQ=255)
attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttttttt < 1:1426688/100‑1 (MQ=255)
tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 2:1668809/100‑1 (MQ=255)
tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 1:910561/100‑1 (MQ=255)
taccccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 1:675327/97‑1 (MQ=255)
tctccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGa < 1:1539400/97‑1 (MQ=255)
atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg > 2:1244872/1‑99 (MQ=255)
tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACTAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGAc < 2:1273833/100‑1 (MQ=255)
cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGAcc > 2:32055/1‑100 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGaag > 2:1539391/1‑97 (MQ=255)
ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCt > 1:961832/1‑100 (MQ=255)
cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCtt < 2:849471/100‑1 (MQ=255)
aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTa > 2:1857906/1‑100 (MQ=255)
cggTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGAACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTa < 2:616414/97‑1 (MQ=255)
cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaa < 2:961828/100‑1 (MQ=255)
gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaa < 2:1446820/100‑1 (MQ=255)
gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaa < 1:1644489/100‑1 (MQ=255)
tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaaa > 1:1725398/1‑100 (MQ=255)
gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAAc < 2:1240422/100‑1 (MQ=255)
cGGGGCTGCACTTAGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACa < 1:1092079/100‑1 (MQ=255)
ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATaa > 1:1460644/1‑100 (MQ=255)
gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAAc < 1:10890/100‑1 (MQ=255)
tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCg > 2:1135068/1‑100 (MQ=255)
cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgag > 1:1047934/1‑100 (MQ=255)
cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgag > 1:676311/1‑100 (MQ=255)
aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgagg > 1:1416219/1‑100 (MQ=255)
ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgaggag < 1:205289/100‑1 (MQ=255)
tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGc > 2:1801788/1‑100 (MQ=255)
gACGAGTAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg < 1:606183/100‑1 (MQ=255)
gACGAGCAAAGGCTTAGAGATCATGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCTGGGG‑GTCTTTGTTGATGTTAAAAACATTACCTTGGATCAg > 2:1396728/1‑100 (MQ=255)
cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc > 2:593777/1‑100 (MQ=255)
cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc < 2:57106/100‑1 (MQ=255)
ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 2:101521/1‑100 (MQ=255)
ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 1:2005815/1‑100 (MQ=255)
ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 1:271858/1‑100 (MQ=255)
aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt < 2:1989985/100‑1 (MQ=255)
ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGatcggaagag > 2:1487984/1‑91 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 2:1052789/100‑1 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGAACCAAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 2:1416210/100‑1 (MQ=255)
ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 1:1963789/99‑1 (MQ=255)
ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa < 2:1606670/100‑1 (MQ=255)
tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg > 2:1876081/1‑100 (MQ=255)
agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc > 2:649829/1‑100 (MQ=255)
agaTCAAGCCTTACCGCCGCAAGGCCCCCGCGGCGAAAGGTCCGGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc < 1:2040122/99‑1 (MQ=255)
agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca < 2:1725385/99‑1 (MQ=255)
gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca > 1:1417844/1‑100 (MQ=255)
gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAAAACAGAACAAGACAATGAATAACAGca > 1:1109326/1‑98 (MQ=255)
aaGCCTTAACGAACTAAGGCCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt < 1:972969/100‑1 (MQ=255)
ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACCATGAAGAACAGCCCAAAATTCt > 2:904317/1‑100 (MQ=255)
ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt > 1:1496279/1‑100 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt < 2:80732/100‑1 (MQ=255)
aCGAACTAAGACCCCCGCACCGAGAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 1:32055/100‑1 (MQ=255)
aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 2:1840974/100‑1 (MQ=255)
aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 2:700804/100‑1 (MQ=255)
aCGAACTAAGACCCCCGCACCGAAAGGTCC‑GGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct < 1:63151/100‑1 (MQ=255)
aaCTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa < 1:1801804/100‑1 (MQ=255)
aaCTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa > 1:1659338/1‑100 (MQ=255)
cTAAGCCCCCCGCCCCGAGAGGGCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa < 1:1857923/99‑1 (MQ=255)
cccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg < 2:271857/100‑1 (MQ=255)
ccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg > 2:226050/1‑100 (MQ=255)
ccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg < 2:1659325/100‑1 (MQ=255)
cccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa > 1:892117/1‑100 (MQ=255)
cACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg > 2:1548723/1‑100 (MQ=255)
aCCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg < 2:300871/100‑1 (MQ=255)
ccGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa > 1:473020/1‑100 (MQ=255)
gAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac < 2:1047929/100‑1 (MQ=255)
gAAAGGTCCGGGG‑TTTTTTTTTGACCTAAAAAACATAACCGAGGAGCAGACAATGAAAAAAAAAAAAAAATTCTGTTTCTCAAGATTCAGGACGGGGa < 2:892114/98‑1 (MQ=255)
gTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact < 2:1220514/100‑1 (MQ=255)
ccGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta < 1:314833/99‑1 (MQ=255)
ccGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt > 1:203690/1‑100 (MQ=255)
cGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCATAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATg < 2:1863975/100‑1 (MQ=255)
|
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG > NC_000913/3950371‑3950562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
