Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,965,149 A→T 76.3% V161E (GTG→GAG)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,1490AT76.3% 311.1 / 85.2 159V161E (GTG→GAG) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base T (70/49);  total (89/70)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

TGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCA  >  NC_000913/3965051‑3965248
                                                                                                  |                                                                                                   
tGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTaccaccac                                                                                                    >  2:2820999/1‑100 (MQ=255)
 gAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTaccacctcc                                                                                                   <  1:2599735/100‑1 (MQ=255)
 gAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTaacacctcc                                                                                                   >  1:846424/1‑100 (MQ=255)
  aGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCAcctcct                                                                                                  <  1:3476935/100‑1 (MQ=255)
  aGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCAcctcct                                                                                                  >  1:2561281/1‑100 (MQ=255)
   ggCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTg                                                                                                 >  2:2442984/1‑100 (MQ=255)
    gcgcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGa                                                                                                >  1:180974/1‑100 (MQ=255)
     cgcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGaa                                                                                               >  1:428966/1‑100 (MQ=255)
     cgcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                                               <  2:1491523/100‑1 (MQ=255)
      gcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAAt                                                                                              >  2:4149492/1‑100 (MQ=255)
      gcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAAt                                                                                              <  2:1360342/100‑1 (MQ=255)
       cTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATg                                                                                             >  1:1595512/1‑100 (MQ=255)
       cTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATg                                                                                             <  2:4109299/100‑1 (MQ=255)
        ttttttGCCGGGGGCATACGGCGGAACGGCCAGCGGATATCTTTAATACAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATg                                                                                             <  2:323940/96‑1 (MQ=255)
        tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATgg                                                                                            <  1:2878522/100‑1 (MQ=255)
        tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATg                                                                                             >  1:2300684/1‑99 (MQ=255)
        tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTAAAt                                                                                              >  1:371182/1‑98 (MQ=255)
         ggTTTGTAGGCGGCATACGGCGGAACTGCCGTCGGAGGTCATTAACAAATCTTAGCTCGTGCATACAAGCGGGTGCGTCGAGTAACACTCCCTGATTgg                                                                                            >  2:2246653/1‑99 (MQ=255)
             tGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCg                                                                                       >  1:2643423/1‑100 (MQ=255)
              gCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGa                                                                                      <  1:331618/100‑1 (MQ=255)
               cAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAg                                                                                     >  1:2211910/1‑100 (MQ=255)
                 ggtagcATACGGCGGAACAGCCAGTGGATATCTTTAATAAGGCGAAGGTCGTCCATGCGATCGGCTTCCTCCGGTACCACCTCCTCGATGGCACCGAgg                                                                                    <  2:1107313/95‑1 (MQ=255)
                 ggcggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAg                                                                                     >  2:4034400/1‑98 (MQ=255)
                     gcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAt                                                                               >  1:2788852/1‑100 (MQ=255)
                      cATACGGCGGAACAGCCAGCGGATATCTTTAATCAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTGACACCTCCTGAATGGCACCGAGGATAAtg                                                                              >  1:807297/1‑100 (MQ=255)
                      cATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtg                                                                              >  2:1021641/1‑100 (MQ=255)
                      cATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtg                                                                              >  2:1996046/1‑100 (MQ=255)
                      cATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtg                                                                              <  1:1658605/100‑1 (MQ=255)
                      cATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAAtg                                                                              >  1:3670341/1‑100 (MQ=255)
                       aTACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgt                                                                             <  2:3892661/100‑1 (MQ=255)
                       aTACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgt                                                                             <  2:1218106/100‑1 (MQ=255)
                        tACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgtg                                                                            <  2:4054464/100‑1 (MQ=255)
                        tACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgtg                                                                            <  1:552418/100‑1 (MQ=255)
                         aCGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATCCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGATAATGTgg                                                                           >  2:1559210/1‑100 (MQ=255)
                          cggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGt                                                                          >  1:2650651/1‑100 (MQ=255)
                            gcggAACAGCCCGCGGATATCTTTAATAACGCCACGATTGTACATGCGCTCGGCTTCGTCCAGTTCCACCACCTGAATGGCACCGAGGTTAATGTGGTTc                                                                        <  2:3479652/100‑1 (MQ=255)
                             cggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCt                                                                       <  1:2759128/100‑1 (MQ=255)
                                 aCAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCtt                                                                   >  1:1337619/1‑100 (MQ=255)
                                  cagccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTg                                                                  >  1:563613/1‑100 (MQ=255)
                                  cagccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTg                                                                  <  1:2966476/100‑1 (MQ=255)
                                  cagccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTg                                                                  >  2:1421567/1‑100 (MQ=255)
                                   agccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTgg                                                                 <  1:3176289/100‑1 (MQ=255)
                                    gccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGc                                                                >  2:3390757/1‑100 (MQ=255)
                                     ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCg                                                               >  1:1066187/1‑100 (MQ=255)
                                     ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCg                                                               <  1:666999/100‑1 (MQ=255)
                                       agcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTa                                                             <  1:2442999/100‑1 (MQ=255)
                                          ggATATCTTTCATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGTACTGAGGTTAATGTGGTTCTGCTTGGCGTAGTc                                                          <  2:2739896/100‑1 (MQ=255)
                                           gATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCa                                                         >  1:579885/1‑100 (MQ=255)
                                             tatCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCact                                                       >  2:2533224/1‑98 (MQ=255)
                                               tCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTa                                                     <  1:2105336/100‑1 (MQ=255)
                                               tCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTa                                                     >  2:3620146/1‑100 (MQ=255)
                                                cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                    >  1:447288/1‑100 (MQ=255)
                                                cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                    >  2:242112/1‑100 (MQ=255)
                                                cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                    >  2:375085/1‑100 (MQ=255)
                                                cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                    >  1:1528105/1‑100 (MQ=255)
                                                cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                    <  2:1845827/100‑1 (MQ=255)
                                                 tttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTACTGTGGTTCTGCTTGGCGTAGTCAATTaaa                                                   >  2:1789900/1‑100 (MQ=255)
                                                 tttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaaa                                                   >  2:2080876/1‑100 (MQ=255)
                                                  ttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAAc                                                  <  2:2155445/100‑1 (MQ=255)
                                                   taataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACg                                                 >  2:572053/1‑100 (MQ=255)
                                                     ataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGCATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcc                                               >  1:1652013/1‑100 (MQ=255)
                                                     ataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcc                                               >  2:894342/1‑100 (MQ=255)
                                                     ataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcc                                               <  1:2067736/100‑1 (MQ=255)
                                                     ataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcc                                               >  1:3690191/1‑100 (MQ=255)
                                                       aaaGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcccc                                             >  2:3413000/1‑100 (MQ=255)
                                                          gCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtg                                          >  1:895361/1‑100 (MQ=255)
                                                          gCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtg                                          >  1:2192410/1‑100 (MQ=255)
                                                           cccAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtgg                                         >  2:1934853/1‑100 (MQ=255)
                                                               gATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGcc                                     <  2:555283/100‑1 (MQ=255)
                                                               gATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGcc                                     <  1:82856/100‑1 (MQ=255)
                                                                 tagTACATGCGATAGGCTTCGTCCAGTACCAGCTCCTGAATGACACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCaa                                   >  1:2811004/3‑100 (MQ=255)
                                                                 tCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCaa                                   >  1:1699302/1‑100 (MQ=255)
                                                                    tACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCa                                <  1:3410557/100‑1 (MQ=255)
                                                                       aTGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGaa                             <  1:101616/100‑1 (MQ=255)
                                                                        tGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAAt                            >  1:115416/1‑100 (MQ=255)
                                                                         gCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATg                           <  1:3821602/100‑1 (MQ=255)
                                                                          cGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                          <  1:1536059/100‑1 (MQ=255)
                                                                          cGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                          >  2:1652919/1‑100 (MQ=255)
                                                                           gATCGGCTTCGTCCAGTACCACCACGTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTc                         <  1:1231859/100‑1 (MQ=255)
                                                                            aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCa                        >  1:3561559/1‑100 (MQ=255)
                                                                                gCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgc                    >  1:306589/1‑100 (MQ=255)
                                                                                gCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgc                    >  1:1770911/1‑100 (MQ=255)
                                                                                gCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgc                    >  1:1790182/1‑100 (MQ=255)
                                                                                gCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgc                    <  1:3827740/100‑1 (MQ=255)
                                                                                 cTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgcc                   <  1:2397028/100‑1 (MQ=255)
                                                                                   tCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCGGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgccgc                 <  1:1107470/100‑1 (MQ=255)
                                                                                    catCCAGTACCACGTCCTGAATGGCACCGAGGTTAATGTGGTACTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCt                >  1:2821922/3‑100 (MQ=255)
                                                                                     gTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCtt               >  2:3074583/1‑100 (MQ=255)
                                                                                     gTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCtt               <  1:1019548/100‑1 (MQ=255)
                                                                                     gTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAGTTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCtt               >  2:1036703/1‑100 (MQ=255)
                                                                                      tCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCttt              >  1:4020183/1‑100 (MQ=255)
                                                                                        cAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTcc            <  1:2094048/100‑1 (MQ=255)
                                                                                          gTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAg          <  2:3075185/100‑1 (MQ=255)
                                                                                          gTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAg          <  1:272134/100‑1 (MQ=255)
                                                                                          gTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAg          >  2:1352086/1‑100 (MQ=255)
                                                                                           tACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGc         <  2:876306/100‑1 (MQ=255)
                                                                                            accacctccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCa        >  2:3761292/1‑100 (MQ=255)
                                                                                            accaccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCa        >  1:3193965/1‑100 (MQ=255)
                                                                                             ccacctccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCAc       <  1:3003192/100‑1 (MQ=255)
                                                                                             ccaccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAGGCCGCTTTCCAGCAc       >  1:4106054/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCA  >  NC_000913/3965051‑3965248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A