Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,965,149 A→T 44.8% V161E (GTG→GAG)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,1490AT44.8% 47.1 / 199.2 148V161E (GTG→GAG) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (39/42);  new base T (38/28);  total (78/70)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 160 total aligned reads displayed.

TTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTT  >  NC_000913/3965050‑3965245
                                                                                                   |                                                                                                
ttGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTaccacca                                                                                                  >  1:2220213/1‑100 (MQ=255)
  gAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTaccaccacc                                                                                                <  2:3140439/100‑1 (MQ=255)
    ggCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTg                                                                                              >  2:1719475/1‑100 (MQ=255)
    ggCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTg                                                                                              >  1:2430853/1‑100 (MQ=255)
    ggCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTg                                                                                              >  2:2195057/1‑100 (MQ=255)
      cgcTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCGCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                                            >  1:2941491/1‑100 (MQ=255)
        cTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATg                                                                                          <  2:3883351/100‑1 (MQ=255)
         tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATgg                                                                                         >  1:3758484/1‑100 (MQ=255)
         tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATgg                                                                                         >  2:3984316/1‑100 (MQ=255)
          ggTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCTTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGc                                                                                        >  2:2933730/1‑100 (MQ=255)
            tttGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCAc                                                                                      <  1:1900388/100‑1 (MQ=255)
             ttGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCAcc                                                                                     >  2:3579323/1‑100 (MQ=255)
             ttGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCAcc                                                                                     <  1:1871487/100‑1 (MQ=255)
              tGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCg                                                                                    >  1:384906/1‑100 (MQ=255)
                cAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAg                                                                                  >  2:1098762/1‑100 (MQ=255)
                cAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAg                                                                                  >  1:613257/1‑100 (MQ=255)
                  ggcggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGt                                                                                >  1:2188816/1‑100 (MQ=255)
                  ggcggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGt                                                                                >  1:3807434/1‑100 (MQ=255)
                       cATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtg                                                                           >  2:1989560/1‑100 (MQ=255)
                        aTACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgt                                                                          >  2:2540022/1‑100 (MQ=255)
                        aTACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAAtgt                                                                          >  2:3408409/1‑100 (MQ=255)
                        aTAAGGGGGAACAGGCAGCGCATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGCCCAGTACCACCACCTGAATGGCACCGAGGTTAAtg                                                                           <  1:3560332/99‑1 (MQ=255)
                          aCGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTgg                                                                        >  2:3420106/1‑100 (MQ=255)
                           cggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGt                                                                       >  1:1137064/1‑100 (MQ=255)
                           cggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGt                                                                       >  2:1465782/1‑100 (MQ=255)
                           cggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGt                                                                       <  1:246493/100‑1 (MQ=255)
                           cggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGt                                                                       >  1:1056659/1‑100 (MQ=255)
                            ggcggAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGtt                                                                      >  1:1901781/1‑100 (MQ=255)
                                     gccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGc                                                             <  1:4241983/100‑1 (MQ=255)
                                      ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCg                                                            >  2:1474671/1‑100 (MQ=255)
                                      ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCg                                                            <  2:3735206/100‑1 (MQ=255)
                                       cagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGctt                                                           <  2:2890237/100‑3 (MQ=255)
                                        agcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTa                                                          <  1:28520/100‑1 (MQ=255)
                                          cGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGt                                                        <  1:3603487/100‑1 (MQ=255)
                                          cGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGt                                                        <  2:219152/100‑1 (MQ=255)
                                           ggATATCTTTCATCGAGCCCAGATCCTACGTGCTCTCCGCTTTGTCCGGTTCCTCCTCCTGAATGGCACCGAGGTTAATGTGCTTCTTCTTGGCGTAGTc                                                       <  1:3906661/100‑1 (MQ=255)
                                                tCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTa                                                  <  1:918407/100‑1 (MQ=255)
                                                 cTTTAATAACGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGACTGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTa                                                  >  1:109778/1‑99 (MQ=255)
                                                 cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                 >  2:587612/1‑100 (MQ=255)
                                                 cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                 >  1:2317848/1‑100 (MQ=255)
                                                 cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaa                                                 >  1:1584737/1‑100 (MQ=255)
                                                 cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTCCTGCTTGGTGCAGTCAAt                                                    >  2:1625359/1‑97 (MQ=255)
                                                 cTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAACGTGGTTCTGCTTGGCGTAGTCAATTaa                                                 >  1:3925896/1‑100 (MQ=255)
                                                  tttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaaa                                                >  2:4259811/1‑100 (MQ=255)
                                                  tttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCCCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTaaa                                                >  1:2716693/1‑100 (MQ=255)
                                                  tttAATAAAGCCCAGATCGCACATGCGATCGGCTTCGTCCAGTACCACCAACTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATGaaa                                                <  1:2015256/100‑1 (MQ=255)
                                                   ttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAAc                                               <  1:1706281/100‑1 (MQ=255)
                                                   ttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAAc                                               <  2:3211626/100‑1 (MQ=255)
                                                    taataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACg                                              <  2:2802622/100‑1 (MQ=255)
                                                    taataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACg                                              >  2:487465/1‑100 (MQ=255)
                                                     aataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGGTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGc                                             >  2:3927364/1‑100 (MQ=255)
                                                      ataaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcc                                            <  2:742062/100‑1 (MQ=255)
                                                       taaAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGccc                                           <  1:1615363/100‑1 (MQ=255)
                                                        aaaGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGcccc                                          >  2:1874354/1‑100 (MQ=255)
                                                         aaGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCg                                         <  2:2676109/100‑1 (MQ=255)
                                                          aGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgt                                        <  1:1276330/100‑1 (MQ=255)
                                                           gCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtg                                       >  2:762624/1‑100 (MQ=255)
                                                             ccAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggt                                     <  2:1503342/100‑1 (MQ=255)
                                                             ccAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggt                                     >  2:1054535/1‑100 (MQ=255)
                                                             ccAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggt                                     >  1:2894099/1‑100 (MQ=255)
                                                              cAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggtg                                    <  2:2065611/100‑1 (MQ=255)
                                                              cAGATCGTACATGCGATCGGCTTCGGCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggtg                                    <  2:1852872/100‑1 (MQ=255)
                                                                   cGTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAAt                               >  1:1518360/1‑100 (MQ=255)
                                                                    gTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATc                              <  1:3168658/100‑1 (MQ=255)
                                                                    gTACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATc                              >  1:2722327/1‑100 (MQ=255)
                                                                    gTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATc                              >  2:2230165/1‑100 (MQ=255)
                                                                    gTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATc                              <  1:346743/100‑1 (MQ=255)
                                                                     tACATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCa                             <  1:1865787/100‑1 (MQ=255)
                                                                      aCATGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAg                            <  1:827235/100‑1 (MQ=255)
                                                                      aCATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAg                            <  1:549035/100‑1 (MQ=255)
                                                                       cATGCTATCGGCTTTTTCAAATACCATCACATGAATGGCACCGATGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCTCGTGGTGCCAATCAg                            <  1:894217/99‑1 (MQ=255)
                                                                         tGCGATCGGCTTCGTGCAGTACCTCCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCGTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATc                              <  1:3168625/95‑1 (MQ=255)
                                                                         tGCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCGCCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAAGGCCCCGTGGTGCCAATCAg                            >  1:2281540/1‑97 (MQ=255)
                                                                          gCGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATg                        <  1:3544946/100‑1 (MQ=255)
                                                                           cGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                       >  2:2780823/1‑100 (MQ=255)
                                                                           cGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                       >  2:599880/1‑100 (MQ=255)
                                                                           cGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                       >  1:3635162/1‑100 (MQ=255)
                                                                           cGATCGGCTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                       >  2:2412362/1‑100 (MQ=255)
                                                                           cGATCGGCTTCGCCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGt                       <  2:2668642/100‑1 (MQ=255)
                                                                            gATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTc                      <  2:640366/100‑1 (MQ=255)
                                                                             aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCa                     <  2:243531/100‑1 (MQ=255)
                                                                             aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCa                     >  2:3968964/1‑100 (MQ=255)
                                                                                ggCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcg                  >  1:47039/1‑100 (MQ=255)
                                                                                ggCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcg                  <  2:2301370/100‑1 (MQ=255)
                                                                                  cTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgcc                <  2:1754354/100‑1 (MQ=255)
                                                                                  cTTCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgcc                >  2:3576390/1‑100 (MQ=255)
                                                                                  cTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCGAATCAGAATGTCAAagcc                >  1:697289/1‑100 (MQ=255)
                                                                                  cTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgcc                <  2:1115605/100‑1 (MQ=255)
                                                                                    tCGTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgccgc              >  2:1813598/1‑100 (MQ=255)
                                                                                    tCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgccgc              <  1:1501873/100‑1 (MQ=255)
                                                                                    tCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCATAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAAcgccgc              <  2:279976/100‑1 (MQ=255)
                                                                                      gTCCAGTACCACCTCCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCtt            >  2:1663148/1‑100 (MQ=255)
                                                                                       tCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCttt           >  2:3484808/1‑100 (MQ=255)
                                                                                         cAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTcc         <  1:2128469/100‑1 (MQ=255)
                                                                                          aGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCa        <  1:776216/100‑1 (MQ=255)
                                                                                            tACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGc      <  2:851361/100‑1 (MQ=255)
                                                                                             agcacctccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCa     >  2:2894623/3‑100 (MQ=255)
                                                                                             accacctccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGc      >  1:1089264/1‑99 (MQ=255)
                                                                                              ccaccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCAc    >  1:4206510/1‑100 (MQ=255)
                                                                                                accaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACtt  >  2:1971143/1‑100 (MQ=255)
                                                                                                   |                                                                                                
TTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTT  >  NC_000913/3965050‑3965245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A