Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 331,668 A→G 49.4% intergenic (+172/‑703) betT → / → yahA choline transporter of high affinity/c‑di‑GMP‑specific phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913331,6680AG49.4% ‑0.5 / 212.5 152intergenic (+172/‑703)betT/yahAcholine transporter of high affinity/c‑di‑GMP‑specific phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (41/36);  new base G (35/40);  total (76/76)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 152 total aligned reads displayed.

CGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAA  >  NC_000913/331571‑331764
                                                                                                 |                                                                                                
cGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAAt                                                                                                >  2:2246244/1‑100 (MQ=255)
     aaGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTc                                                                                            <  2:3063387/99‑1 (MQ=255)
      aGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCaa                                                                                          >  1:4091213/1‑100 (MQ=255)
       gCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAAt                                                                                         >  1:396113/1‑100 (MQ=255)
       gCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAAt                                                                                         >  1:3075069/1‑100 (MQ=255)
        cccTTAGTATCTATTGATGATTACCAGCCAACAGATAATAAAAAACAAAAGCACTATTGCAGCCCAAAACCTACATATGGGCTGTTGCGACTGTTCACTa                                                                                        >  2:1714387/1‑100 (MQ=255)
        cccTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTAAATTTGGGCTGTTGCGAATGTTCAATa                                                                                        >  2:1156367/1‑100 (MQ=255)
         ccTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATaa                                                                                       >  1:3647211/1‑100 (MQ=255)
         ccTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATaa                                                                                       >  2:3012027/1‑100 (MQ=255)
          cTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAg                                                                                      <  1:2396084/100‑1 (MQ=255)
             aGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGttt                                                                                   <  1:3478922/100‑1 (MQ=255)
             aGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGttt                                                                                   <  1:2855034/100‑1 (MQ=255)
              gTATCTATTGATGATTAGCAGACAACAGATAATAAAAAAGAAAAGGACTATTGCAGCCTAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGttt                                                                                   <  2:3836135/99‑1 (MQ=255)
               tATCTATTGATGATTACCAGACAACAAATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAg                                                                                 <  2:1882620/100‑1 (MQ=255)
                  cTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCt                                                                              >  1:3304333/1‑100 (MQ=255)
                   tagtgatgaTTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTc                                                                               <  2:897178/95‑1 (MQ=255)
                    aTTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTa                                                                            >  1:1441600/1‑100 (MQ=255)
                    aTTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTa                                                                            >  1:394191/1‑100 (MQ=255)
                     ttGATGATTACCAGACAACAGATAATAAAAAAGCAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTTTTGCGAATGTTCAATAAGTTTAGTCTTAt                                                                           >  2:2483438/1‑100 (MQ=255)
                     ttGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTAt                                                                           >  1:144691/1‑100 (MQ=255)
                      tgatgaTTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTAtt                                                                          >  1:3429881/1‑100 (MQ=255)
                      tgatgaTTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGCCTGTTGCGGATGTTCAATAAGTTTAGTCTTAt                                                                           >  2:264358/1‑99 (MQ=255)
                       gatgatTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTAttt                                                                         >  2:3142523/1‑100 (MQ=255)
                        atgatTAACAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTa                                                                        <  2:3087153/100‑1 (MQ=255)
                         tgatTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTaa                                                                       <  1:3349213/100‑1 (MQ=255)
                          gatTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAAt                                                                      >  1:3631448/1‑100 (MQ=255)
                          gatTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAAt                                                                      >  2:1313512/1‑100 (MQ=255)
                           atTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATg                                                                     <  1:2377791/100‑1 (MQ=255)
                            ttACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGt                                                                    >  1:1958359/1‑100 (MQ=255)
                             tACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTa                                                                   <  1:3108947/100‑1 (MQ=255)
                             tACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTa                                                                   <  1:3009030/100‑1 (MQ=255)
                              aCCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTaa                                                                  >  1:128700/1‑100 (MQ=255)
                               ccAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTaaa                                                                 >  2:1728372/1‑100 (MQ=255)
                               ccAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTaca                                                                 >  2:2504719/1‑98 (MQ=255)
                                cAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAat                                                                <  2:629305/100‑1 (MQ=255)
                                cAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGAGGATGTTCAATAAGTCTAGTCTTATTTAATGTAAat                                                                >  2:3475834/1‑100 (MQ=255)
                                 aGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTAAATAAGTTTAGTCTTATTTAATGTAAata                                                               >  1:4026841/1‑100 (MQ=255)
                                 aGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAata                                                               >  1:1116681/1‑100 (MQ=255)
                                    caacaGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATACGTTTAGTCTTATTTAATGTAAATATTg                                                            >  1:1108426/1‑100 (MQ=255)
                                    caacaGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTg                                                            >  2:204174/1‑100 (MQ=255)
                                     aacaTATAATAAAAAAGAAAAGAACTATTGCAGCTCAAAACCTACATTTGGGATGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGc                                                           <  2:235157/100‑1 (MQ=255)
                                     aacaGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGc                                                           <  1:1253828/100‑1 (MQ=255)
                                       caGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTg                                                         >  1:4115359/1‑100 (MQ=255)
                                       caGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTg                                                         <  2:3506561/100‑1 (MQ=255)
                                            aataaAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCAt                                                    <  1:1703464/100‑1 (MQ=255)
                                             ataaAAAAGAAAAGCACTATTGCAGCCCAAAACCTACAATTGGGCTGTTGCGAGTGGTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGAGCAtt                                                   >  2:2547685/1‑100 (MQ=255)
                                             ataaAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCAtt                                                   >  1:3722531/1‑100 (MQ=255)
                                              taaAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCAttt                                                  >  1:1847323/1‑100 (MQ=255)
                                              taaAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCAttt                                                  <  2:1068614/100‑1 (MQ=255)
                                              taaAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGACTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCAttt                                                  >  2:2428232/1‑100 (MQ=255)
                                               aaaaaaGAAAAGAACTATTGCAGCCCAAAACCTACATTTAGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTg                                                 <  2:3346268/100‑1 (MQ=255)
                                                aaaaaGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGa                                                >  1:2464205/1‑100 (MQ=255)
                                                 aaaagaaaagAACTATTGCAGCCCAAAACCTTCATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGaa                                               <  1:1775556/100‑1 (MQ=255)
                                                   aagaaaagaaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAAt                                             <  2:1198729/100‑1 (MQ=255)
                                                    agaaaagaaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATg                                            <  1:975126/100‑1 (MQ=255)
                                                    agaaaagaaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATg                                            <  1:2111274/100‑1 (MQ=255)
                                                      aagagaaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAGTGTTCAATAAGTTTAGTCTTAGTTAATGTAAATATTGCTGATCATTTGAAATGAc                                          <  2:1256298/97‑1 (MQ=255)
                                                       aaataaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACg                                         <  2:251059/100‑1 (MQ=255)
                                                          gaaCTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCat                                      <  2:1554454/100‑1 (MQ=255)
                                                                ttGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCa                                <  2:3647187/100‑1 (MQ=255)
                                                                ttGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCa                                <  1:712199/100‑1 (MQ=255)
                                                                 tGCAGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCAt                               >  2:4011547/1‑100 (MQ=255)
                                                                 tGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCAt                               <  1:690519/100‑1 (MQ=255)
                                                                  gCAGCCCATGAGCTACATTTGGTCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGAGCATTTGAAATGACGCATTATTCATg                              <  2:1356483/100‑1 (MQ=255)
                                                                  gCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATg                              >  1:264878/1‑100 (MQ=255)
                                                                  gCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATg                              <  2:2113389/100‑1 (MQ=255)
                                                                   cAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATg                              <  2:899959/99‑1 (MQ=255)
                                                                    aGCCCAAAACCTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATgag                            >  1:1635611/1‑100 (MQ=255)
                                                                     gCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATgaga                           >  1:372010/1‑100 (MQ=255)
                                                                     gCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATgaga                           >  1:3411915/1‑100 (MQ=255)
                                                                       ccAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGaaa                         >  2:1516125/1‑100 (MQ=255)
                                                                        cAAAACCTACATTTGGGCTGGTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAt                        <  1:75062/100‑1 (MQ=255)
                                                                          aaaCCTACATTTTGGCTGTTGCTGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtg                       <  2:1906349/99‑1 (MQ=255)
                                                                           aaCCGACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtgtg                     <  2:2698493/100‑1 (MQ=255)
                                                                             ccTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTa                   >  2:3808318/1‑100 (MQ=255)
                                                                             ccTACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTa                   >  2:2120473/1‑100 (MQ=255)
                                                                             ccTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTa                   <  1:812079/100‑1 (MQ=255)
                                                                             ccTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTa                   >  1:2361301/1‑100 (MQ=255)
                                                                              cTACATTTGGGCTGTTGCGAATGTTCAATAAGGTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtgtg                     <  2:145993/97‑1 (MQ=255)
                                                                               tACATTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATc                 <  2:3575275/100‑1 (MQ=255)
                                                                                aCATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCg                <  1:1849647/100‑1 (MQ=255)
                                                                                  aTTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTa              >  1:1222977/1‑100 (MQ=255)
                                                                                  aTTTGGGCTGTTGCGGATGTTCAATAAGTTTAGTCTTACTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTa              <  2:2244271/100‑1 (MQ=255)
                                                                                   tttGGGCTGTTGCGAATGTTCGATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaa             >  2:396891/1‑100 (MQ=255)
                                                                                   tttGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaa             >  2:876421/1‑100 (MQ=255)
                                                                                     tGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAAt           <  1:3465215/100‑1 (MQ=255)
                                                                                       ggCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCa         <  1:3126364/100‑1 (MQ=255)
                                                                                       ggCTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCa         <  2:431104/100‑1 (MQ=255)
                                                                                         cTGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAAc       <  1:7893/100‑1 (MQ=255)
                                                                                         cTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCaa        >  2:3173179/1‑99 (MQ=255)
                                                                                          tGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACt      <  2:3301967/100‑1 (MQ=255)
                                                                                          tGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACt      <  2:2846851/100‑1 (MQ=255)
                                                                                          tGTTGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACt      <  2:230600/100‑1 (MQ=255)
                                                                                          tGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACt      >  1:1381427/1‑100 (MQ=255)
                                                                                          tGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTAATGAGAAATGTGTATTTTAAATCaaat      >  2:2042940/1‑98 (MQ=255)
                                                                                            ttGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa    >  2:509211/1‑100 (MQ=255)
                                                                                            ttGCGAATAGTCAATAATTGTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa    <  1:1786655/100‑1 (MQ=255)
                                                                                             tGCGGATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGaa   <  1:2702905/100‑1 (MQ=255)
                                                                                             tGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGaa   >  1:975754/1‑100 (MQ=255)
                                                                                             tGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGaa   <  2:810811/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAA  >  NC_000913/331571‑331764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A