Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,782,076 T→C 52.0% V217V (GTA→GTG)  ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,0760TC52.0% 19.7 / 216.7 169V217V (GTA→GTG) ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (37/43);  new base C (35/52);  total (72/97)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

Only 100 of 173 total aligned reads displayed.

GTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACT  >  NC_000913/2781978‑2782174
                                                                                                  |                                                                                                  
gTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCAtata                                                                                                   <  1:3606182/100‑1 (MQ=255)
  gCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACAct                                                                                                 <  1:2432711/100‑1 (MQ=255)
    caccaGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATActct                                                                                               >  1:3152025/1‑100 (MQ=255)
     accaGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCtt                                                                                              >  1:3250120/1‑100 (MQ=255)
      ccaGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCttt                                                                                             <  1:2539309/100‑1 (MQ=255)
      ccaGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCttt                                                                                             >  1:1045942/1‑100 (MQ=255)
      ccaGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCttt                                                                                             >  2:920971/1‑100 (MQ=255)
       caGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTg                                                                                            <  1:1790184/100‑1 (MQ=255)
        aGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGc                                                                                           <  1:3479058/100‑1 (MQ=255)
        aGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGc                                                                                           <  2:3750486/100‑1 (MQ=255)
          aaTAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTc                                                                                         <  1:1262177/100‑1 (MQ=255)
            atcttatGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCAc                                                                                       <  2:2539237/96‑1 (MQ=255)
             aGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCAcc                                                                                      <  1:1362702/100‑1 (MQ=255)
                taggCGTATTTTCTGCCGTTGATTTACTATTCAGCGACTGAGTTCCGCCTTCGATCGTCGTGGCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGc                                                                                    <  2:3313187/96‑1 (MQ=255)
                   gCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCAtt                                                                                >  2:848913/1‑100 (MQ=255)
                    cGTATTTTTTGCCGTTGACTTACTATTCAGAGTCTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCAttn                                                                               <  2:3948056/100‑2 (MQ=255)
                    cGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCAtt                                                                                >  2:3349020/1‑99 (MQ=255)
                     gTATTTTTTTTTGTTGTCTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCAtttt                                                                              >  2:1652372/1‑100 (MQ=255)
                      tATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCAttttt                                                                             <  2:1243005/100‑1 (MQ=255)
                        ttttttGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTaa                                                                           <  1:421918/100‑1 (MQ=255)
                         tttttGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAAt                                                                          <  1:213678/100‑1 (MQ=255)
                           tttGCCGTTGACTTACTATTCAGAGACTGATTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATg                                                                         <  2:1764093/99‑1 (MQ=255)
                            ttGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgt                                                                       <  1:2523161/100‑1 (MQ=255)
                             tGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACCGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgtt                                                                      >  2:3830837/1‑100 (MQ=255)
                               ccGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgttgt                                                                    >  1:1633251/1‑100 (MQ=255)
                                 gTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGgttgttg                                                                  <  1:1493305/100‑1 (MQ=255)
                                  ttGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGgttgttgt                                                                 <  1:2604082/100‑1 (MQ=255)
                                    gACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCTCGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGttt                                                               <  2:205891/100‑1 (MQ=255)
                                    gACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGttt                                                               >  1:3195480/1‑100 (MQ=255)
                                     aCTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTc                                                              <  2:4072569/100‑1 (MQ=255)
                                     aCTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTc                                                              <  1:2200518/100‑1 (MQ=255)
                                      cTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCt                                                             >  1:2529919/1‑100 (MQ=255)
                                      cTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCt                                                             >  1:2757070/1‑100 (MQ=255)
                                       ttACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTa                                                            <  1:3221763/100‑1 (MQ=255)
                                        tACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTAc                                                           >  1:1235480/1‑100 (MQ=255)
                                        tACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTAc                                                           <  1:3901637/100‑1 (MQ=255)
                                          cTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACtg                                                         <  2:2847440/100‑1 (MQ=255)
                                          cTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACt                                                          <  2:2981758/99‑1 (MQ=255)
                                           tATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACtgt                                                        <  1:691578/100‑1 (MQ=255)
                                            aTTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACtgtg                                                       >  1:767428/1‑100 (MQ=255)
                                             ttCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGc                                                      <  1:4017865/100‑1 (MQ=255)
                                             ttCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGc                                                      <  1:3044873/100‑1 (MQ=255)
                                               cAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctc                                                    >  1:932790/1‑100 (MQ=255)
                                               cAGAGACTGAGTTCCGCCTTCAATCGTCGTGTACAGCGCTCGTCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctc                                                    <  2:2904105/100‑1 (MQ=255)
                                                  agaCTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGt                                                 >  1:831324/1‑100 (MQ=255)
                                                    cttccgatctgCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTc                                               <  1:3459753/90‑1 (MQ=255)
                                                    aCTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTc                                               <  1:2185387/100‑1 (MQ=255)
                                                    aCTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTc                                               >  2:1504990/1‑100 (MQ=255)
                                                     cTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCa                                              <  1:2320782/100‑1 (MQ=255)
                                                     cTGAGTTCCGCCTTCAATCGTCGTGTCCAACGCACGGCTCTCATACACTCTTTGCTCAACGCAGTTTGTAGTAGTTGTTGTTTCTACTGTGatct                                                   >  2:3773246/1‑95 (MQ=255)
                                                      tGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCaa                                             <  2:3199769/100‑1 (MQ=255)
                                                      tGACTTCCGCCTTCAATCGGCGTGTCAAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCaa                                             <  1:3934290/100‑1 (MQ=255)
                                                       gAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                            >  1:1119372/1‑100 (MQ=255)
                                                       gAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                            <  2:3090554/100‑1 (MQ=255)
                                                       gAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                            <  1:2116601/100‑1 (MQ=255)
                                                       gAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                            <  1:941539/100‑1 (MQ=255)
                                                       gAGTTCCGCCGTCAATCGTCGTGTCCAGCGCACGGCTCTCATCCACTCTTTGCTCACCGCCATGTTTAATGGTGCTTGTTTATACTGTGCTCTGTTAAAc                                            <  2:2295825/100‑1 (MQ=255)
                                                       gAGTTCCGCATTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                            <  2:1603718/100‑1 (MQ=255)
                                                         gTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACAt                                          >  2:2850/1‑100 (MQ=255)
                                                           tCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATAc                                        >  2:194211/1‑100 (MQ=255)
                                                           tCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATAc                                        <  2:492620/100‑1 (MQ=255)
                                                            ccGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGATCAACATACt                                       >  2:2710947/1‑100 (MQ=255)
                                                            ccGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACt                                       >  2:3910189/1‑100 (MQ=255)
                                                              gCCTTCAATCGTCGTGTCCATCGCACGTCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTGGTTTCTACTGTGCTCTGTTCAACATAc                                        <  2:1109608/97‑1 (MQ=255)
                                                              gCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCagatcggaag                                     >  2:3459731/1‑91 (MQ=255)
                                                               ccTTCAATCGTCGTGTCCAGTGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTc                                    <  1:1691023/100‑1 (MQ=255)
                                                                 ttCAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGa                                  >  1:2343521/1‑100 (MQ=255)
                                                                   cAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacc                                >  2:557224/1‑100 (MQ=255)
                                                                   cAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacc                                <  2:2613758/100‑1 (MQ=255)
                                                                   cAATCGTCGTGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacc                                >  1:206395/1‑100 (MQ=255)
                                                                    aaTCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacca                               >  2:668764/1‑100 (MQ=255)
                                                                    aaTCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacca                               <  2:859698/100‑1 (MQ=255)
                                                                     aTCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacc                                <  2:2564642/98‑1 (MQ=255)
                                                                       cgtcgtGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGAccacca                            >  2:516009/1‑100 (MQ=255)
                                                                       cgtcgtGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGAccacca                            <  1:2374205/100‑1 (MQ=255)
                                                                        gtcgtGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGAccacca                            >  2:852500/1‑99 (MQ=255)
                                                                        gtcgtGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCAt                           >  1:2986014/1‑100 (MQ=255)
                                                                         tcgtGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCAtt                          <  2:2946119/100‑1 (MQ=255)
                                                                         tcgtGTCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCAtt                          <  1:1930981/100‑1 (MQ=255)
                                                                           gtgtCGAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTa                        <  1:48787/100‑1 (MQ=255)
                                                                            tgtCCAGTGCACGGCTCTAATATATTCTTTTCTCACCTTTATTTTTTATGTTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACGACCAtt                          <  2:227487/97‑1 (MQ=255)
                                                                             gtCCAGCGCACGGCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATg                      <  1:1308165/100‑1 (MQ=255)
                                                                               ccAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGt                    >  1:2195765/1‑100 (MQ=255)
                                                                               ccAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGt                    <  1:848919/100‑1 (MQ=255)
                                                                               ccAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGt                    >  1:4121084/1‑100 (MQ=255)
                                                                                 aGCGCACGTCTCTCATAAACTCTTTGCTCACCGCCTTTTTTACTAGTTGTTGGTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTtg                  <  2:1300651/100‑1 (MQ=255)
                                                                                  gcgcACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTtg                  <  2:2741314/99‑1 (MQ=255)
                                                                                    gcACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTgtgt               <  1:1884222/100‑1 (MQ=255)
                                                                                        ggCTCTCATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGt           <  1:2090551/100‑1 (MQ=255)
                                                                                         gCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGtt          >  2:265144/1‑100 (MQ=255)
                                                                                          ctctCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTg         >  1:2411931/1‑100 (MQ=255)
                                                                                            ctcATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGcc       <  2:146079/100‑1 (MQ=255)
                                                                                             tcATATACTCTTTGCTCACCGTCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCa      <  2:1177330/100‑1 (MQ=255)
                                                                                             tcATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCa      <  1:554181/100‑1 (MQ=255)
                                                                                             tcATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCa      >  2:1313160/1‑100 (MQ=255)
                                                                                             tcATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCa      >  1:1753270/1‑100 (MQ=255)
                                                                                              cATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAg     >  2:1519241/1‑100 (MQ=255)
                                                                                              cATACACTCTTTGCTCACCGCCATTTTTTTTTTTTTTTTTTTCTACTGAGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAg     >  2:433085/1‑100 (MQ=255)
                                                                                              cATACACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAg     <  1:2928914/100‑1 (MQ=255)
                                                                                                  taCTCTTTGCTCACCGCCATTTTTAATGGTTGTAGTTTCTACTTAGCTCTGTTCAACATAATTTCGACCAGCATTTATCAGTGTGTTCCCTGCCAGa    >  2:3588453/1‑97 (MQ=255)
                                                                                                  |                                                                                                  
GTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACT  >  NC_000913/2781978‑2782174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A