Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,955 G→A 62.1% R120H (CGC→CAC)  tas → putative NADP(H)‑dependent aldo‑keto reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,9550GA62.1% 130.5 / 189.8 177R120H (CGC→CAC) tasputative NADP(H)‑dependent aldo‑keto reductase
Reads supporting (aligned to +/- strand):  ref base G (29/38);  new base A (46/64);  total (75/102)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 179 total aligned reads displayed.

GTGAGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCC  >  NC_000913/2971858‑2972054
                                                                                                 |                                                                                                   
gTGAGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGcc                                                                                                   >  1:3474370/1‑100 (MQ=255)
 tGAGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCt                                                                                                  <  2:278690/100‑1 (MQ=255)
   aGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTAc                                                                                                <  1:4126418/100‑1 (MQ=255)
   aGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTAc                                                                                                <  1:572550/100‑1 (MQ=255)
    gCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACa                                                                                               >  1:2472094/1‑100 (MQ=255)
     cGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAg                                                                                              <  2:2194101/100‑1 (MQ=255)
     cGGACCGTCCTGCAATACTGAGACGTGTATCCGCCCGGATCAGGCGCTGGATCTTAAGAATCTCCGCGAAGCGCTGCATGTCAGCCTCAAGCACCTACAg                                                                                              <  2:2839878/100‑1 (MQ=255)
       gACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGAc                                                                                            <  1:2970042/100‑1 (MQ=255)
        aCCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACt                                                                                           <  1:3667889/100‑1 (MQ=255)
        aCCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACt                                                                                           <  1:1935266/100‑1 (MQ=255)
         ccGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTg                                                                                          <  1:2066648/100‑1 (MQ=255)
         ccGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTg                                                                                          <  1:4012675/100‑1 (MQ=255)
         ccGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTg                                                                                          >  1:4073532/1‑100 (MQ=255)
           gTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGAt                                                                                        >  1:3390966/1‑100 (MQ=255)
            tCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGAtt                                                                                       <  1:2350250/100‑1 (MQ=255)
            tCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGAtt                                                                                       <  1:3832542/100‑1 (MQ=255)
             cgcgCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTa                                                                                      <  2:4073505/100‑1 (MQ=255)
              gcgcAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTAc                                                                                     >  1:282675/1‑100 (MQ=255)
               cgcAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTTGATCGGAAGAATATCCGCGCAGCGCTGCATGACAGCCTCCAGCGCCTACAGACTGATTaac                                                                                    <  2:2249459/100‑3 (MQ=255)
               cgcAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTAcc                                                                                    >  1:2119674/1‑100 (MQ=255)
               cgcAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTAcc                                                                                    >  2:691967/1‑100 (MQ=255)
                 cAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTc                                                                                  <  2:79780/100‑1 (MQ=255)
                 cAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTc                                                                                  <  2:261356/100‑1 (MQ=255)
                   ataatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGa                                                                                <  1:3010842/100‑1 (MQ=255)
                   ataatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGa                                                                                <  1:3279858/100‑1 (MQ=255)
                   ataatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGa                                                                                >  2:1963774/1‑100 (MQ=255)
                   ataatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGa                                                                                <  1:2545613/100‑1 (MQ=255)
                    taatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGAt                                                                               >  1:3880648/1‑100 (MQ=255)
                     aatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATc                                                                              <  1:542233/100‑1 (MQ=255)
                     aatGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATc                                                                              <  1:2539535/100‑1 (MQ=255)
                      atGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCt                                                                             <  1:375741/100‑1 (MQ=255)
                      atGACAAGGGCATCCGCCCGGATCAGGCGCGGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCt                                                                             <  1:3536108/100‑1 (MQ=255)
                       tGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCtt                                                                            <  1:2177194/100‑1 (MQ=255)
                       tGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCtt                                                                            >  1:291383/1‑100 (MQ=255)
                        gACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCttt                                                                           >  1:108858/1‑100 (MQ=255)
                         aCAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTa                                                                          <  1:1551311/100‑1 (MQ=255)
                          cAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTAt                                                                         >  1:132743/1‑100 (MQ=255)
                          cAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTAt                                                                         >  1:805713/1‑100 (MQ=255)
                           aaGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATc                                                                        <  2:650209/100‑1 (MQ=255)
                            aGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCa                                                                       <  2:3285144/100‑1 (MQ=255)
                            aGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCa                                                                       >  1:3954239/1‑100 (MQ=255)
                            aGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCa                                                                       <  2:3167423/100‑1 (MQ=255)
                             gggCATCCGCCCGAATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAg                                                                      >  2:3030942/1‑100 (MQ=255)
                              ggCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAgg                                                                     >  2:511197/1‑100 (MQ=255)
                              ggCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAgg                                                                     >  1:2195453/1‑100 (MQ=255)
                               gCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGt                                                                    >  2:1894207/1‑100 (MQ=255)
                                cATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTg                                                                   >  1:3307556/1‑100 (MQ=255)
                                cATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTg                                                                   >  2:557822/1‑100 (MQ=255)
                                cATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTg                                                                   <  1:3055582/100‑1 (MQ=255)
                                cATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTg                                                                   <  2:150642/100‑1 (MQ=255)
                                cATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTg                                                                   <  1:2312799/100‑1 (MQ=255)
                                        cGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGcc                                                           <  2:3307536/100‑1 (MQ=255)
                                         ggATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCg                                                          >  2:39370/1‑100 (MQ=255)
                                          gATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCAGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGc                                                         <  1:2509095/100‑1 (MQ=255)
                                           aTCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCa                                                        <  2:146194/100‑1 (MQ=255)
                                           aTCAGGCGCTGGATCGGAAGAATACCCGCGAAGCGCAGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGAACTGGCCGc                                                         >  2:2357556/1‑99 (MQ=255)
                                                gcgcTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGcc                                                   >  1:966998/1‑100 (MQ=255)
                                                gcgcTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGcc                                                   >  2:1438731/1‑100 (MQ=255)
                                                  gcTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCg                                                 <  2:3023316/100‑1 (MQ=255)
                                                    tgcaTCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGAc                                               <  2:1941927/97‑1 (MQ=255)
                                                    tGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGAc                                               >  2:2431745/1‑100 (MQ=255)
                                                     ggATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGAc                                               >  1:482962/1‑99 (MQ=255)
                                                       aTCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCaa                                            >  2:2568154/1‑100 (MQ=255)
                                                        tCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAAc                                           <  1:3427938/100‑1 (MQ=255)
                                                        tCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAAc                                           <  1:584405/100‑1 (MQ=255)
                                                          ggAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACt                                          <  1:602016/99‑1 (MQ=255)
                                                          ggAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTg                                         <  2:1639550/100‑1 (MQ=255)
                                                          ggAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTg                                         >  2:1320730/1‑100 (MQ=255)
                                                          ggAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTg                                         >  2:1186503/1‑100 (MQ=255)
                                                           gaagaaTATCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGc                                        <  1:2675270/100‑1 (MQ=255)
                                                              gaaTATCCGCGAAGCGCTGCATGACAGCCTCAATCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTc                                     <  2:3798756/100‑1 (MQ=255)
                                                              gaaTATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTc                                     <  1:3703212/100‑1 (MQ=255)
                                                               aaTATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCg                                    <  1:2585057/100‑1 (MQ=255)
                                                                atatCCGCGAAGCGCTGCATGACAGCCTCAAGTACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCgg                                   <  1:2985125/100‑1 (MQ=255)
                                                                atatCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCgg                                   <  1:727888/100‑1 (MQ=255)
                                                                  atCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCa                                 <  1:852600/100‑1 (MQ=255)
                                                                  atCCGCGAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCa                                 <  1:653273/100‑1 (MQ=255)
                                                                    ccGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCaaa                               >  2:2660599/1‑100 (MQ=255)
                                                                     cgcgAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAAc                              >  1:1679395/1‑100 (MQ=255)
                                                                     cgcgAAGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAAc                              <  1:3026416/100‑1 (MQ=255)
                                                                       cgAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTc                            <  2:2059258/100‑1 (MQ=255)
                                                                         aaGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCgg                          >  2:2689481/1‑100 (MQ=255)
                                                                          aGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGt                         <  1:2268640/100‑1 (MQ=255)
                                                                          aGCGCTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGt                         <  1:306345/100‑1 (MQ=255)
                                                                          aGCGCTGCATGACAGCCGCAAGCGCCTACAGACTGAGTACCTCGATCTTGATCAGGGGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGt                         <  2:3284138/100‑1 (MQ=255)
                                                                            cgcTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGtt                        >  2:3577108/1‑99 (MQ=255)
                                                                             ggtGCAGGACAGCCTCAAGCACCGCCAGGCTGGATACCTCATTCTTTATCAGGTGCACTGGCCGCAGCGTCCGGCCAACTGCTGCTGCAAACTCGGt                         <  2:2350284/95‑1 (MQ=255)
                                                                             gcTGCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGCCCAACTGCTTCGGCAAACTCGGTtat                      >  2:3332629/1‑100 (MQ=255)
                                                                                gCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGCCCAACTGCTTCGGCAAACTCGGTTATAg                    >  2:1628722/1‑99 (MQ=255)
                                                                                gCATGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAg                    <  2:1281805/99‑1 (MQ=255)
                                                                                  aTGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTg                 <  2:1829718/100‑1 (MQ=255)
                                                                                  aTGACAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTg                 <  2:4137139/100‑1 (MQ=255)
                                                                                     aCAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGAc              >  1:391883/1‑100 (MQ=255)
                                                                                      cAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACg             <  2:1505196/100‑1 (MQ=255)
                                                                                      cAGCCTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACg             >  1:3195816/1‑100 (MQ=255)
                                                                                        gCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGa           >  2:350326/1‑100 (MQ=255)
                                                                                         ccTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACCCGGTTATAGCTGGACgg            >  1:1705270/1‑98 (MQ=255)
                                                                                          cTCAAGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGCtt         >  2:57273/1‑100 (MQ=255)
                                                                                           tCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTc        >  1:2641529/1‑100 (MQ=255)
                                                                                             aaGCACCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTg      <  1:1137198/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GTGAGCGGACCGTCGCGCAATAATGACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCC  >  NC_000913/2971858‑2972054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A