Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,655 Δ1 bp 54.9% coding (120/633 nt) yqeH → putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,6540G.54.9% 7.4 / inf 153coding (119/633 nt)yqeHputative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (28/41);  new base . (41/43);  total (69/84)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 167 total aligned reads displayed.

ATAAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTCGATTTTTC  >  NC_000913/2987555‑2987753
                                                                                                   |                                                                                                   
ataAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTAtgtg                                                                                                     >  1:583064/1‑100 (MQ=255)
ataAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTAtgtg                                                                                                     >  1:3472927/1‑100 (MQ=255)
 taAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTAtgtgc                                                                                                    >  2:2304013/1‑99 (MQ=255)
 taAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTgg                                                                                                    >  1:3289069/1‑100 (MQ=255)
 taAAAGTCTTACATGCAATAGTTGCTCAATGCAATGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATCGCGCAAACTCGTGTTTTTGTAtgtgc                                                                                                    >  1:737229/1‑99 (MQ=255)
   aaaGTCTTACATGCAATAGTTGCTTCTTGCGTTGTCATATAATGCCAGAAGAGTCACCGCGTTTGGAGTATTGCGCAAACTCGTGTTTGTGTCTGTGGcc                                                                                                  <  2:2167463/100‑1 (MQ=255)
   aaaGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTAtgtgccc                                                                                                  >  1:2630558/1‑97 (MQ=255)
   aaaGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGcc                                                                                                  <  1:2230847/100‑1 (MQ=255)
    aaGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGcccg                                                                                                 <  2:2468331/100‑2 (MQ=255)
     aGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCga                                                                                               <  1:843129/100‑1 (MQ=255)
      gTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCga                                                                                               <  2:2998022/100‑1 (MQ=255)
        cTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCgaag                                                                                             >  1:82556/1‑100 (MQ=255)
        cTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCgaag                                                                                             >  1:1357401/1‑100 (MQ=255)
          tACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCgaagaa                                                                                           >  2:3741662/1‑100 (MQ=255)
           aCATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCTCCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCGCGAAGAAAg                                                                                         <  2:177980/100‑1 (MQ=255)
           aCATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAg                                                                                         <  2:1989846/100‑1 (MQ=255)
            cATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGt                                                                                        <  1:555886/100‑1 (MQ=255)
            cATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGt                                                                                        <  1:3765206/100‑1 (MQ=255)
             aTGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGtt                                                                                       <  2:3631796/100‑1 (MQ=255)
              tgtaaTAGTTGCTCAATGCATTTGCAAATACTGCCATAAGAGTCACCGAGTTTTTAGTATTTCGCAACCTCGTGTTTCTGTATGT‑GCCCGAAGAAAGTTc                                                                                      <  2:3831948/97‑1 (MQ=255)
              tGCAATAGTTGCTCAATGTATTGCCAAATAATGCCAGAAGAGTCATCGCGTTTGCAGTATTGCGCAACCTCGTGTTTTTGTATGTGGCCCGAAGAAAGtt                                                                                       <  2:1998984/100‑1 (MQ=255)
              tGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTc                                                                                      >  1:2329709/1‑100 (MQ=255)
              tGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTc                                                                                      >  1:3598978/1‑100 (MQ=255)
               gCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCa                                                                                     <  2:931566/100‑1 (MQ=255)
                cAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCat                                                                                    >  1:646248/1‑100 (MQ=255)
                    aGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATAtt                                                                                 <  2:913262/100‑1 (MQ=255)
                       tGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTa                                                                              >  2:2094732/1‑100 (MQ=255)
                        gCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAAt                                                                            >  1:2450738/1‑100 (MQ=255)
                         gtCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTGTTGTATGT‑GCCTGAAGAAAGTTCATATTTTAATc                                                                           >  2:606872/2‑100 (MQ=255)
                         cTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAAt                                                                            <  2:1133204/100‑1 (MQ=255)
                          tCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATc                                                                           >  1:2085464/1‑100 (MQ=255)
                          tCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATc                                                                           <  2:82555/100‑1 (MQ=255)
                          tCAATGCAGTGCCAAATAATGCCAGAAGAGTCACCGCGCTTGCAGTATTGCGCAAACTTGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCg                                                                          <  2:2251403/100‑1 (MQ=255)
                           cAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCg                                                                          <  1:3122998/100‑1 (MQ=255)
                           cAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCg                                                                          >  1:2984742/1‑100 (MQ=255)
                           cAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTAATGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGACAGTTCATATTTTAATCgt                                                                         >  2:995158/1‑100 (MQ=255)
                             aTGCATTGCTAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑TCTCGAAGAAAGTTCATATTTTAATCgtgg                                                                       >  2:588569/1‑100 (MQ=255)
                             aTGCATTGCCAATTAGTGCCAGGAGAGTCACCGCGTTTTCAGCATTACTCAAACTCGTGTTTTTGTATGT‑ACCCGAGGAAAGTTTATATTTTTATCgtgg                                                                       >  2:1908360/1‑100 (MQ=255)
                             aTGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtgg                                                                       >  2:3467615/1‑100 (MQ=255)
                             aTGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtgg                                                                       <  2:287419/100‑1 (MQ=255)
                             aTGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtgg                                                                       <  1:662361/100‑1 (MQ=255)
                                cATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtggtgt                                                                    <  2:3660562/100‑1 (MQ=255)
                                  ttGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCgtggtgtg                                                                   <  2:2130386/100‑1 (MQ=255)
                                   tGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtggtgtggt                                                                 <  2:1416837/100‑1 (MQ=255)
                                   tGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCgtggtgtggt                                                                 <  2:2095147/100‑1 (MQ=255)
                                    gCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCgtggtgtggt                                                                 >  2:122526/1‑100 (MQ=255)
                                    gCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCgtggtgtggt                                                                 >  1:4111832/1‑100 (MQ=255)
                                    gCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCgtggtgtggt                                                                 >  2:3483419/1‑100 (MQ=255)
                                     ccAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAg                                                               >  2:2607248/1‑100 (MQ=255)
                                      cAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGa                                                              <  1:1308772/100‑1 (MQ=255)
                                       aaaTAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGa                                                              <  1:4084115/100‑1 (MQ=255)
                                        aataatGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAg                                                            <  2:528908/100‑1 (MQ=255)
                                         ataatGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAgg                                                           <  2:1412980/100‑1 (MQ=255)
                                          taatGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAgg                                                           <  1:2843770/100‑1 (MQ=255)
                                          taatGCCAGAAGAGTCACCGCGTTTGAAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCTTGGTGTGGTAGAAGGt                                                          <  2:1651299/100‑1 (MQ=255)
                                            atGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTa                                                         <  1:1184403/100‑1 (MQ=255)
                                             tGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTAt                                                        >  2:4095840/1‑100 (MQ=255)
                                             tGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATc                                                       >  2:725730/1‑100 (MQ=255)
                                             tGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATc                                                       <  1:829134/100‑1 (MQ=255)
                                             tGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATc                                                       <  1:2295043/100‑1 (MQ=255)
                                               ccAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCt                                                      >  2:3597876/1‑100 (MQ=255)
                                               ccAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGG‑CCGAAGGAAGGTCATATTTTAATCGTGGTTTGGTAGAAGGTagc                                                       >  1:2250853/1‑96 (MQ=255)
                                               ccAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCtt                                                     <  1:726680/100‑1 (MQ=255)
                                                cAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTa                                                    <  1:1041110/100‑1 (MQ=255)
                                                 agaagaGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTa                                                    <  1:2004876/100‑1 (MQ=255)
                                                    agagTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACa                                                 >  2:3377937/1‑100 (MQ=255)
                                                      agTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACaaaa                                              >  2:3273526/1‑100 (MQ=255)
                                                       gTCACCGCGTTTGCAGTATTGCACAAACTCGTGTTTTTGTATGT‑GCACGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACaaaaa                                             <  2:1021035/100‑1 (MQ=255)
                                                         cACCGCGTTTGCAGTATTGCGCAAACTCGTGTTATTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTCGTGTGGTAGAAGGTATCTTAACAAAAAAc                                           >  2:2480423/1‑100 (MQ=255)
                                                          aCCGCGTTTGCAGTACTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAAc                                           <  1:1992273/100‑1 (MQ=255)
                                                           ccGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACca                                         >  2:3577673/1‑100 (MQ=255)
                                                            cgcgTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACcac                                        >  2:4052393/1‑100 (MQ=255)
                                                             gcgTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACcac                                        <  2:2565595/100‑1 (MQ=255)
                                                             gcgTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCTCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATTTTAACAAAAAACtac                                        >  2:2390462/1‑99 (MQ=255)
                                                             gcgTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACcaca                                       <  1:2132982/100‑1 (MQ=255)
                                                               gTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACaa                                      >  1:1799016/1‑100 (MQ=255)
                                                               gTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAAt                                     >  2:1752471/1‑100 (MQ=255)
                                                                  tGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCg                                  >  1:278072/1‑100 (MQ=255)
                                                                  tGCAGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCg                                  >  2:2924937/1‑100 (MQ=255)
                                                                     aGTATTGCGCAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCgaga                               <  1:3245860/100‑1 (MQ=255)
                                                                           gcgcAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAg                          >  2:3952133/1‑100 (MQ=255)
                                                                           gcgcAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGc                         <  2:3158268/100‑1 (MQ=255)
                                                                           gcgcAAACTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGc                         >  1:1899270/1‑100 (MQ=255)
                                                                               aaaCTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGa                      <  2:3582697/100‑1 (MQ=255)
                                                                               aaaCTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGat                     <  1:1021937/100‑1 (MQ=255)
                                                                               aaaCTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGat                     >  1:3593044/1‑100 (MQ=255)
                                                                                 aCTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGata                    <  1:3264292/100‑1 (MQ=255)
                                                                                 aCTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGata                    <  1:3172785/100‑1 (MQ=255)
                                                                                  cTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGatata                  >  2:3154071/1‑100 (MQ=255)
                                                                                  cTCGTGTTTTTGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGatata                  >  2:3813017/1‑100 (MQ=255)
                                                                                   tCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCTGatata                  >  2:228458/1‑100 (MQ=255)
                                                                                    cGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGatat                   <  2:2808558/98‑1 (MQ=255)
                                                                                      tgtTTTTGTATGT‑GCCCGAAGAAAGGTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATAtttt              <  1:3867901/100‑1 (MQ=255)
                                                                                         ttttGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTg            <  1:1925539/100‑1 (MQ=255)
                                                                                          tttGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATAtttt              <  1:3012441/97‑1 (MQ=255)
                                                                                          tttGTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTc          <  2:2076766/100‑1 (MQ=255)
                                                                                          nttGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGataaatatatg            >  2:2605151/2‑94 (MQ=255)
                                                                                             gTATGT‑GCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTCGAt       >  1:1728066/1‑100 (MQ=255)
                                                                                               aTGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTCGAtt      <  1:3097175/100‑1 (MQ=255)
                                                                                                   ggCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTCGATTTTTc  >  2:2139644/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATAAAAGTCTTACATGCAATAGTTGCTCAATGCATTGCCAAATAATGCCAGAAGAGTCACCGCGTTTGCAGTATTGCGCAAACTCGTGTTTTTGTATGTGGCCCGAAGAAAGTTCATATTTTAATCGTGGTGTGGTAGAAGGTATCTTAACAAAAAACCACAATGCGAGATTAAGCGGATATATTTTTGTCGATTTTTC  >  NC_000913/2987555‑2987753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A