Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F56 I0 R1	312	170.3	8276235	93.7%	7754832	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,017,118	C→A	52.0%	R353R (CGC→CGA)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,118	0	C	A	52.0%	26.9 / 274.9	172	R353R (CGC→CGA)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base C (41/41); new base A (50/40); total (91/81)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-03

Only 100 of 178 total aligned reads displayed.

CACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGG  >  NC_000913/3017019‑3017215

cACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTcgc                                                                                                   <  1:4008604/100‑1 (MQ=255)

  ccATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCgagc                                                                                                 <  1:2933752/100‑1 (MQ=255)

   cATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGcc                                                                                                >  2:2315674/1‑100 (MQ=255)

      caacaaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCttt                                                                                             >  1:1285665/1‑100 (MQ=255)

       aacaaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTg                                                                                            >  1:3591537/1‑100 (MQ=255)

       aacaaTAAAGGCGCACTGCCTGGTGAAGAGATGTATAAGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCttt                                                                                             >  2:2213259/1‑99 (MQ=255)

         caaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGAc                                                                                          >  2:2278600/1‑100 (MQ=255)

         caaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGAc                                                                                          >  1:3825582/1‑100 (MQ=255)

            tAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGc                                                                                       <  2:498405/100‑1 (MQ=255)

              aaGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCaa                                                                                     >  1:3694595/1‑100 (MQ=255)

               aGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGc                                                                                       <  2:3815893/97‑1 (MQ=255)

               aGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCaaa                                                                                    >  2:1276718/1‑100 (MQ=255)

               aGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCaa                                                                                     >  2:3354073/1‑99 (MQ=255)

                ggCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAAc                                                                                   >  2:1081314/1‑100 (MQ=255)

                ggCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAAc                                                                                   <  2:1152659/100‑1 (MQ=255)

                ggCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAAc                                                                                   <  1:1339136/100‑1 (MQ=255)

                     aCTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGccc                                                                              <  2:3267355/100‑1 (MQ=255)

                      cTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCa                                                                             >  2:1379671/1‑100 (MQ=255)

                       tGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCAt                                                                            >  1:3799064/1‑100 (MQ=255)

                          cTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTc                                                                         <  1:1370425/100‑1 (MQ=255)

                            ggTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCtt                                                                       <  1:3520150/100‑1 (MQ=255)

                              tGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCAGTTCTTAt                                                                     <  1:2160587/100‑1 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCCGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTcc                                                                  >  2:550619/1‑100 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTcc                                                                  <  2:751305/100‑1 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTcc                                                                  <  2:1498534/100‑1 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCACACTGCCCATTTCTTATTcc                                                                  >  2:3214122/1‑100 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTcc                                                                  >  1:2708715/1‑100 (MQ=255)

                                 agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTcc                                                                  <  2:3892026/100‑1 (MQ=255)

                                   agaTGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCATCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCgg                                                                <  2:102391/100‑1 (MQ=255)

                                    gaTGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggt                                                               <  1:3217492/100‑1 (MQ=255)

                                      tGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggtgg                                                             <  1:699528/100‑1 (MQ=255)

                                      tGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggtgg                                                             >  2:81233/1‑100 (MQ=255)

                                      tGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggtgg                                                             >  1:3674071/1‑100 (MQ=255)

                                      tGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggtgg                                                             <  2:2209515/100‑1 (MQ=255)

                                       gTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggtggt                                                            >  2:1969178/1‑100 (MQ=255)

                                        tataTGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGgtggtg                                                           >  2:243051/1‑100 (MQ=255)

                                        tataTGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGgtggtg                                                           >  2:2541283/1‑100 (MQ=255)

                                        tataTGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGgtggtg                                                           >  1:444857/1‑100 (MQ=255)

                                         atatGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGc                                                          >  1:3049731/1‑100 (MQ=255)

                                          tatGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGcc                                                         <  1:2278613/100‑1 (MQ=255)

                                           atGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCa                                                        <  2:2485036/100‑1 (MQ=255)

                                            tGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAg                                                       <  1:4121767/100‑1 (MQ=255)

                                            tGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAg                                                       >  1:1618864/1‑100 (MQ=255)

                                            tGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAg                                                       >  1:330227/1‑100 (MQ=255)

                                             gTCGGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGt                                                      >  2:3705186/1‑100 (MQ=255)

                                             gTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGt                                                      >  1:3633380/1‑100 (MQ=255)

                                              tCAGGCCGTGGGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGCCGGCCAATTGCCCATTTCTTATTCCGGTGGTGCCAGTc                                                     <  2:2716171/100‑1 (MQ=255)

                                               cAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCCGTCa                                                    >  2:291717/1‑100 (MQ=255)

                                               cAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCa                                                    <  1:3091857/100‑1 (MQ=255)

                                                  gCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCt                                                 <  1:1143676/100‑1 (MQ=255)

                                                     gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc                                              <  1:1918621/100‑1 (MQ=255)

                                                     gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc                                              <  1:2010452/100‑1 (MQ=255)

                                                     gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc                                              <  1:324978/100‑1 (MQ=255)

                                                     gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc                                              <  1:3989854/100‑1 (MQ=255)

                                                     gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc                                              <  2:4078014/100‑1 (MQ=255)

                                                       gcgcTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTa                                            >  2:3217020/1‑100 (MQ=255)

                                                       gcgcTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTa                                            >  1:2498645/1‑100 (MQ=255)

                                                         gcTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATc                                          >  1:3495108/1‑100 (MQ=255)

                                                          cTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATcc                                         <  2:856656/100‑1 (MQ=255)

                                                           tGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcg                                        >  2:771793/1‑100 (MQ=255)

                                                           tGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcg                                        <  2:2656867/100‑1 (MQ=255)

                                                            gTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgc                                       >  1:1206243/1‑100 (MQ=255)

                                                            gTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgc                                       <  1:1180610/100‑1 (MQ=255)

                                                            gTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCCCTCTAGCCTTTGACGGCAAACTGACCATTGCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgc                                       <  2:2254593/100‑1 (MQ=255)

                                                             ttCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgcg                                      <  1:1104281/100‑1 (MQ=255)

                                                              tCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTACTCCGGCGGTGCCAGTCCGCTGCCTATcc                                         >  2:3138077/1‑96 (MQ=255)

                                                              tCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGa                                     >  1:1510389/1‑100 (MQ=255)

                                                              tCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGa                                     >  1:835602/1‑100 (MQ=255)

                                                                ccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGata                                   >  1:1103422/1‑100 (MQ=255)

                                                                ccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGata                                   >  1:2525501/1‑100 (MQ=255)

                                                                ccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGat                                    >  1:3960829/1‑99 (MQ=255)

                                                                ccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGata                                   >  1:121754/1‑100 (MQ=255)

                                                                ccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGata                                   >  2:2796625/1‑100 (MQ=255)

                                                                  gCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAtt                                 >  1:4105265/1‑100 (MQ=255)

                                                                  gCTCTCCATCAATATTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAtt                                 >  2:2559638/1‑100 (MQ=255)

                                                                     ctcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAttttt                              <  1:1880155/100‑1 (MQ=255)

                                                                     ctcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAttttt                              >  2:3024923/1‑100 (MQ=255)

                                                                      tcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTg                             <  1:243052/100‑1 (MQ=255)

                                                                      tcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTg                             <  2:616813/100‑1 (MQ=255)

                                                                      tcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTg                             >  1:541958/1‑100 (MQ=255)

                                                                       ccATCAATGTTGCAGCAGTTCTCTCGCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAttttt                              <  1:3873011/98‑1 (MQ=255)

                                                                        cATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt                           <  1:3697768/100‑1 (MQ=255)

                                                                        cATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt                           >  1:3443156/1‑100 (MQ=255)

                                                                        cATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGCTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt                           >  2:4107838/1‑100 (MQ=255)

                                                                        cATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt                           <  1:1495988/100‑1 (MQ=255)

                                                                         aTCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATa                          >  2:1850501/1‑100 (MQ=255)

                                                                          tCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATAc                         <  2:3620726/100‑1 (MQ=255)

                                                                            aaTGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAg                       >  2:1814811/1‑100 (MQ=255)

                                                                             atcttGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAgg                      <  2:2509741/97‑1 (MQ=255)

                                                                                   cagcagTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCg                >  2:976988/1‑100 (MQ=255)

                                                                                   cagcagTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCg                >  1:2900756/1‑100 (MQ=255)

                                                                                      cagTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCCGCTGACTATCCGCGCTATTTTTGATACAGGTATTCGccc             >  2:1771141/1‑100 (MQ=255)

                                                                                          nctctctcGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTAtt         >  2:2057614/2‑100 (MQ=255)

                                                                                           ctctctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTa        >  2:2815346/1‑100 (MQ=255)

                                                                                            tctctcGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTAc       <  2:1305140/100‑1 (MQ=255)

                                                                                               ctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTa     >  2:4068175/1‑99 (MQ=255)

                                                                                               ctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTAt    >  1:1695183/1‑100 (MQ=255)

                                                                                               ctcGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTAt    >  2:1864799/1‑100 (MQ=255)

                                                                                                 cgcgCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATgg  <  1:3066519/100‑1 (MQ=255)

                                                                                                 cgcgCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATgg  >  2:2566163/1‑100 (MQ=255)

CACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGG  >  NC_000913/3017019‑3017215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A