Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F142 I0 R1 175 91.2 4812830 94.5% 4548124 99.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,993 T→A 67.8% I116N (ATC→AAC)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,9930TA67.8% 109.8 / 68.6 84I116N (ATC→AAC) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (14/13);  new base A (27/29);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTG  >  NC_000913/2352897‑2353091
                                                                                                |                                                                                                  
aCTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                                                                                 <  1:1206555/100‑1 (MQ=255)
   gCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAgcgc                                                                                              <  2:987823/100‑1 (MQ=255)
        gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAg                                                                                         >  1:381702/1‑100 (MQ=255)
        gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAg                                                                                         >  1:1075068/1‑100 (MQ=255)
         aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGc                                                                                        >  1:859217/1‑100 (MQ=255)
           ccaaccatCGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCta                                                                                      <  2:550035/100‑1 (MQ=255)
            caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGc                                                                                        <  1:811444/97‑1 (MQ=255)
            caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtat                                                                                     <  1:830298/100‑1 (MQ=255)
             atccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCt                                                                                       <  1:522898/95‑1 (MQ=255)
             aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCACCGCAGAAGCtata                                                                                    >  2:2304974/1‑100 (MQ=255)
             aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata                                                                                    >  1:1920417/1‑100 (MQ=255)
             aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata                                                                                    <  1:284544/100‑1 (MQ=255)
                caacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAc                                                                                 <  2:795027/100‑1 (MQ=255)
                     gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgca                                                                            <  2:1075058/100‑1 (MQ=255)
                        tGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgcagca                                                                         <  2:1243513/100‑1 (MQ=255)
                          tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAg                                                                       <  1:1481083/100‑1 (MQ=255)
                           ttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgc                                                                      <  1:2193092/100‑1 (MQ=255)
                            tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcg                                                                     <  1:773836/100‑1 (MQ=255)
                            tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcg                                                                     >  1:997037/1‑100 (MQ=255)
                            tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCCCATAAGCTATAGACCCgcagca                                                                         >  2:140060/1‑96 (MQ=255)
                              taaCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTAGTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg                                                                   >  1:1623862/3‑100 (MQ=255)
                                aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAgcgcgc                                                                  >  2:1732454/1‑99 (MQ=255)
                                aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa                                                                 >  2:1733469/1‑100 (MQ=255)
                                 cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt                                                                >  2:1327636/1‑100 (MQ=255)
                                 cccTGCCGGAAGATGACCTCTCCTTCCAGACCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt                                                                >  2:1005921/1‑100 (MQ=255)
                                  ccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt                                                               <  2:633053/100‑1 (MQ=255)
                                       cGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAATAATCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGTGCGCATTATc                                                            >  2:1683977/1‑98 (MQ=255)
                                         gAAGATGACCCCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAATCTATAGACCCGCAGCAAGCGCGCATTATCGAAc                                                        <  2:1571641/100‑1 (MQ=255)
                                          aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAcc                                                       <  2:1174469/100‑1 (MQ=255)
                                          aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAcc                                                       >  2:1560808/1‑100 (MQ=255)
                                          aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAACTATAGAACCGCAGCAAGCGCGCATTATCGaa                                                         >  1:1378434/1‑98 (MQ=255)
                                           aGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCt                                                      <  1:1652202/100‑1 (MQ=255)
                                            gATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTg                                                     >  1:211196/1‑100 (MQ=255)
                                             aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGACCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc                                                    >  2:2254386/1‑100 (MQ=255)
                                             aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc                                                    <  1:1495674/100‑1 (MQ=255)
                                              tGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGcc                                                   <  1:1603981/100‑1 (MQ=255)
                                              tGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGcc                                                   >  1:1124994/1‑100 (MQ=255)
                                                aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt                                                 >  2:1723233/1‑100 (MQ=255)
                                                aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCg                                                  >  1:592149/1‑99 (MQ=255)
                                                aCCTCTCCTTCCAGGCCACTTTTATTAGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt                                                 >  1:1459678/1‑100 (MQ=255)
                                                 ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                <  2:369728/100‑1 (MQ=255)
                                                  ctctCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTa                                               <  2:859208/100‑1 (MQ=255)
                                                   tctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTaa                                              >  1:39025/1‑100 (MQ=255)
                                                    ctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTaa                                              >  2:138753/1‑99 (MQ=255)
                                                    ctcCTTCCAGGCCACTTTTATTCGCCCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAc                                             <  2:2250974/100‑1 (MQ=255)
                                                     tcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAcc                                            >  1:1230013/1‑100 (MQ=255)
                                                       cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGCTCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                          >  2:362084/1‑100 (MQ=255)
                                                       cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                          >  1:200162/1‑100 (MQ=255)
                                                        ttCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCgg                                         <  1:964835/100‑1 (MQ=255)
                                                         tCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGc                                        >  1:141737/1‑100 (MQ=255)
                                                          ccAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGc                                        <  1:2145868/99‑1 (MQ=255)
                                                          ccAGGCCACCTTTATTCGCGCCTGCGAAGCAGCAGGGAACACCGCAGAAGCTATACCCCCGCAGCCAGCGCGCATTATCGAACCTGCCGTTCACCCGGCa                                       >  2:2229947/1‑100 (MQ=255)
                                                            aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCAc                                      <  2:1465745/99‑1 (MQ=255)
                                                            aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACt                                     <  2:1949652/100‑1 (MQ=255)
                                                             ggCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTg                                    <  2:1229838/100‑1 (MQ=255)
                                                              gCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGa                                   <  1:1952791/100‑1 (MQ=255)
                                                               ccACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGAt                                  >  2:2229222/1‑100 (MQ=255)
                                                                  cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                               >  2:951761/1‑100 (MQ=255)
                                                                  cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                               >  2:1050893/1‑100 (MQ=255)
                                                                  cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                               <  1:2046336/100‑1 (MQ=255)
                                                                    tttATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgcg                             <  2:1929951/100‑1 (MQ=255)
                                                                       actCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg                          >  1:2227558/3‑100 (MQ=255)
                                                                       aTTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg                          >  1:2299933/1‑100 (MQ=255)
                                                                        ttCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGt                         <  1:2080560/100‑1 (MQ=255)
                                                                        ttCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgc                              >  2:1805889/1‑95 (MQ=255)
                                                                         tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGt                         <  1:1043402/99‑1 (MQ=255)
                                                                         tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg                        <  2:1634280/100‑1 (MQ=255)
                                                                         tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg                        <  1:2199851/100‑1 (MQ=255)
                                                                         tCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg                        <  2:67616/100‑1 (MQ=255)
                                                                          cgcgCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa                       >  2:1953714/1‑100 (MQ=255)
                                                                            cgcCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGaaa                     <  2:1550674/100‑1 (MQ=255)
                                                                                  cGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGTGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCg               >  1:1092738/1‑100 (MQ=255)
                                                                                   gaagaaGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCgg              <  1:1727102/100‑1 (MQ=255)
                                                                                   gaagaaGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCgg              <  2:1459888/100‑1 (MQ=255)
                                                                                    aagaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGa             <  1:1203189/100‑1 (MQ=255)
                                                                                     agaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt            >  1:2381566/1‑100 (MQ=255)
                                                                                     agaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt            <  1:120476/100‑1 (MQ=255)
                                                                                      gaagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg           >  1:1498219/1‑100 (MQ=255)
                                                                                        agCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGc         >  2:164649/1‑100 (MQ=255)
                                                                                          cAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCAc       >  2:1586383/1‑100 (MQ=255)
                                                                                            gggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCg     >  1:291197/1‑100 (MQ=255)
                                                                                             ggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGt    >  2:2350333/1‑100 (MQ=255)
                                                                                              gATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCTAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGtt   <  2:1529319/100‑1 (MQ=255)
                                                                                               aTCAGAGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTg  <  2:492453/100‑1 (MQ=255)
                                                                                                |                                                                                                  
ACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTG  >  NC_000913/2352897‑2353091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A