Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F142 I0 R1 175 91.2 4812830 94.5% 4548124 99.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,060 C→T 27.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT27.8% 368.0 / 157.3 232intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (91/76);  new base T (23/42);  total (114/118)
Fisher's exact test for biased strand distribution p-value = 1.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01

Only 100 of 233 total aligned reads displayed.

TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295962‑4296159
                                                                                                  |                                                                                                   
  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC                                                                                                  <  1:961900‑M1/100‑1 (MQ=255)
  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC                                                                                                  <  2:1384096‑M1/100‑1 (MQ=255)
   aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCT                                                                                                 >  1:123761‑M1/1‑100 (MQ=255)
    cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC                                                                                                  <  1:1841928‑M1/98‑1 (MQ=255)
     gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGA                                                                                               <  2:2338553‑M1/100‑1 (MQ=255)
      cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT                                                                                              >  1:1006476‑M1/1‑100 (MQ=255)
      cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT                                                                                              <  1:1463345‑M1/100‑1 (MQ=255)
       gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                             >  1:781234/1‑100 (MQ=32)
       gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATCTTGTAGGCCGGATAAGGCGTTTAGGCTGCATCCGACATCAACGCCT                                                                                                 >  2:1149910‑M1/1‑96 (MQ=255)
         cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCACATCCGACATCAACGCCTGATGCg                                                                                           >  1:569105/1‑100 (MQ=17)
           tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                         <  1:1718169/100‑1 (MQ=33)
            aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTGGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                        >  2:1067992/1‑100 (MQ=12)
            aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                        >  2:1363352/1‑100 (MQ=33)
             tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                       <  1:2021789/100‑1 (MQ=18)
              cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                      >  2:1737012/1‑100 (MQ=18)
              cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  1:1799518/1‑100 (MQ=32)
              cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  2:1718520/1‑100 (MQ=32)
               aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACGAATGCGGGATGCGAGGcct                                                                                     >  2:32454/1‑98 (MQ=11)
                  ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCACCGCCTGATGCGACGCTTGc                                                                                   >  1:267484/1‑99 (MQ=255)
                  ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                  <  2:860842/100‑1 (MQ=25)
                  ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                  >  1:396591/1‑100 (MQ=25)
                   cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg                                                                                 >  1:1247246/1‑100 (MQ=38)
                       gCCAGACAGCGCAATAGCCTTATTTAGCGTTATTTTGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                             <  2:712737/100‑1 (MQ=255)
                       gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                             <  2:2114896/100‑1 (MQ=25)
                       gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                             <  1:2164554/100‑1 (MQ=25)
                        ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            <  1:1646174/100‑1 (MQ=25)
                        ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                                                                            >  1:995708/1‑100 (MQ=255)
                          aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                          <  1:2073930‑M2/100‑2 (MQ=255)
                          aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                          <  2:280168‑M2/100‑2 (MQ=255)
                          aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                          >  2:2048022‑M2/1‑99 (MQ=255)
                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTat                                                                         >  1:42224‑M2/1‑98 (MQ=255)
                            aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTatc                                                                        >  2:2007385‑M2/1‑97 (MQ=255)
                             cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatca                                                                       <  1:2210077‑M2/100‑5 (MQ=255)
                             cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTatca                                                                       >  1:1871897‑M2/1‑96 (MQ=255)
                              aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcag                                                                      >  1:657682‑M2/1‑95 (MQ=255)
                               gcgcAATCGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTACGCCTCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTatcag                                                                      >  2:602411‑M2/1‑94 (MQ=255)
                               gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATaagacgcgttaagcgtcgcatcagg                                                                     <  1:1954605‑M1/100‑26 (MQ=255)
                               gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcagg                                                                     >  1:1065335‑M2/1‑94 (MQ=255)
                               gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcagg                                                                     <  2:836909‑M2/100‑7 (MQ=255)
                               gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcagg                                                                     <  1:1353587‑M2/100‑7 (MQ=255)
                               gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCCTatcagg                                                                     <  1:2221401‑M2/100‑7 (MQ=255)
                                cgcAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcaggc                                                                    >  2:33685‑M2/1‑93 (MQ=255)
                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATaagacgcggcaagcgtcgcatcaggc                                                                    <  1:1730445‑M1/100‑27 (MQ=255)
                                 gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcaggc                                                                    <  1:539027‑M2/99‑8 (MQ=255)
                                 gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcaggc                                                                    <  2:1009853‑M2/99‑8 (MQ=255)
                                 gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  1:757971/100‑1 (MQ=25)
                                 gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  2:804945/100‑1 (MQ=38)
                                 gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  2:1686854/100‑1 (MQ=255)
                                  cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTatcagacct                                                                  <  2:657677‑M2/100‑10 (MQ=255)
                                  cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATaagacgcgttaagcgtcgcatcaggcgt                                                                  <  2:1006465‑M1/100‑29 (MQ=255)
                                  cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                  >  2:926593/1‑100 (MQ=17)
                                  cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                  >  1:1823391/1‑100 (MQ=255)
                                   aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                 <  2:377901/100‑1 (MQ=255)
                                    aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATaagacgcgttaagcgtcgcatcaggcgttg                                                                <  2:1648029‑M1/100‑31 (MQ=255)
                                      atcctcgtttAACGTCATATTGTAGGCAGGATAAGGCGTTTACGCCGCATCCGACATTAACGCCTGATGCGACGCTTGACGCGTCTTATCAggtc                                                                   >  2:1992585/8‑93 (MQ=11)
                                       gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                >  2:1945902/1‑97 (MQ=255)
                                        ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                            <  2:2150571/100‑1 (MQ=255)
                                        ccTGATTTAGCGTGATTTTGTAGGTCCGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATaagacgcggcaagcgtcgcatcaggcattgatg                                                             <  2:792826‑M1/99‑34 (MQ=255)
                                          tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATaagacgcgttaagcgtcgcatcaggcgttgatgtc                                                           <  2:2112314‑M1/99‑36 (MQ=255)
                                                 gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa                                                   >  1:2374104/1‑100 (MQ=255)
                                                 gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCa                                                   >  2:1196434/1‑100 (MQ=18)
                                                    tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAggcttgg                                                     >  2:228750/1‑89 (MQ=255)
                                                         ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                           <  1:1279332/100‑1 (MQ=255)
                                                         ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                           <  2:504014/100‑1 (MQ=40)
                                                         ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                           >  2:904824/1‑100 (MQ=40)
                                                            tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                        <  1:716221/100‑1 (MQ=255)
                                                            gatgTCGGATGCGGCATAAACGCCTTATTCTACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGCCCTACGTCAGGCAGTGCACTAGCCTGAtt                                        >  2:1666865/4‑100 (MQ=0)
                                                               gTCGCATAAGGCATCTACACGACATCCGACACCAACGCTACACGAGACGCTTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                     <  2:569102/100‑1 (MQ=2)
                                                                 cGGATAAGGGGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                   <  1:1366676/100‑1 (MQ=255)
                                                                   gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg                                 <  1:1782983/100‑1 (MQ=40)
                                                                     tAAGGCGTTTACGCCGCATCCGATATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                <  2:2237426/99‑1 (MQ=255)
                                                                     tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGAt                               >  1:443369/1‑100 (MQ=255)
                                                                        ggCGTTTACGGCGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                             <  2:1038524/99‑1 (MQ=255)
                                                                        ggCGTTTACGCCGCATCCGTCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATATAGCGTCAtttt                            >  2:1288674/1‑100 (MQ=255)
                                                                         gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                           >  1:1143700/1‑100 (MQ=255)
                                                                          cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                          >  1:620683/1‑100 (MQ=255)
                                                                          cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                          <  1:301507/100‑1 (MQ=40)
                                                                          cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                          <  2:240898/100‑1 (MQ=40)
                                                                           gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                         <  1:1146285/100‑1 (MQ=255)
                                                                           gTTTACGCCGCATAAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                         <  1:2106559/100‑1 (MQ=255)
                                                                            tttACGCCGGACACGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                        <  2:355144/100‑1 (MQ=11)
                                                                            tttACGCCGCATCCGACATCAACGCTTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                        <  1:1611895/100‑1 (MQ=255)
                                                                            tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                         <  1:926604/99‑1 (MQ=255)
                                                                                cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                    >  2:1504261/1‑100 (MQ=40)
                                                                                 gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGCGATTTTGTAGGTCgg                   >  1:981826/1‑100 (MQ=255)
                                                                                 gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg                   >  1:1665608/1‑100 (MQ=38)
                                                                                 gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg                   >  1:1565893/1‑100 (MQ=38)
                                                                                   cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                 >  2:1144134/1‑100 (MQ=38)
                                                                                        ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc            >  2:401288/1‑100 (MQ=38)
                                                                                        ccGACATCAACGCCTGATGCGACGCTTGCCACGCCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATCAg              >  2:1574365/1‑98 (MQ=11)
                                                                                         cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg           <  1:1431458/100‑1 (MQ=38)
                                                                                         cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg           >  1:804954/1‑100 (MQ=38)
                                                                                           aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt         <  1:1399163/100‑1 (MQ=38)
                                                                                           aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt         <  2:1672966/100‑1 (MQ=35)
                                                                                           aCATAAACGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt         <  1:291319/100‑1 (MQ=255)
                                                                                              tCAACGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGTGTGATTTTGTAGGTCGGATAAGGAGtt         >  2:385249/1‑97 (MQ=255)
                                                                                              tCAACGCCTGATGCGACGCTTAACGCGTCTTatcaggcgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggcta      <  2:433969‑M2/100‑70 (MQ=255)
                                                                                                 aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc   <  1:2286413/100‑1 (MQ=31)
                                                                                                 aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc   >  2:1318130/1‑100 (MQ=31)
                                                                                                  cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg  >  1:1697392/1‑100 (MQ=11)
                                                                                                  |                                                                                                   
TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295962‑4296159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A